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The map label for this gene is srlE [H]

Identifier: 222524724

GI number: 222524724

Start: 1838428

End: 1838814

Strand: Reverse

Name: srlE [H]

Synonym: Chy400_1451

Alternate gene names: 222524724

Gene position: 1838814-1838428 (Counterclockwise)

Preceding gene: 222524725

Following gene: 222524723

Centisome position: 34.9

GC content: 46.77

Gene sequence:

>387_bases
ATGACTGAGGTGAAAAAGTACCGCAAGGTTCGGATTAAGAAGGGACCGAAGGGTTGGGGGGGGCCTCTCATTATTGAACC
GAAACCGGGAAGGGATCTCATTTATTCAGTAACTGGTGGCGGTATTCATCCTCTGGCTCAACATATCGCTAATCTGACTG
GTGGGCGACCGTTTGATGGGTTTAAATCAAAGGCCGATTTTAGTGAAATTGCGGTTGCTGTGATCGATTGTGGAGGTACT
GCGCGCATTGGCGTCTATCCTATGAAAAAAGTACCGACCGTTGATATCTATCCAACCTCGCCTTCTGGTCCATTGATGCG
CTTTATCACTGAAGAATATTTTGTCTCGGGTGTAAGACCGGAAGATGTGGAATTGATCGATGAGTAA

Upstream 100 bases:

>100_bases
CGCTTGGATCTTCAGCGTTGGTCTTTGATCTTTGAAGGGTATGAAGCAACTTTGTCTCAAGCTTCATACCCGAATCAAAT
CAAGAAAGGAGGATATGTCT

Downstream 100 bases:

>100_bases
TACTAGCGGGCATTCGCATGATCAAATTTTCAGCGACTATTTCTGCAATTGGTCCGCTTGTCGACGAGTTTTGTTCGGCA
GGGATAATGGTTTTCTTCGG

Product: Sorbitol phosphotransferase protein II domain-containing protein

Products: NA

Alternate protein names: EII-Gut; PTS system glucitol/sorbitol-specific EIIB component [H]

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MTEVKKYRKVRIKKGPKGWGGPLIIEPKPGRDLIYSVTGGGIHPLAQHIANLTGGRPFDGFKSKADFSEIAVAVIDCGGT
ARIGVYPMKKVPTVDIYPTSPSGPLMRFITEEYFVSGVRPEDVELIDE

Sequences:

>Translated_128_residues
MTEVKKYRKVRIKKGPKGWGGPLIIEPKPGRDLIYSVTGGGIHPLAQHIANLTGGRPFDGFKSKADFSEIAVAVIDCGGT
ARIGVYPMKKVPTVDIYPTSPSGPLMRFITEEYFVSGVRPEDVELIDE
>Mature_127_residues
TEVKKYRKVRIKKGPKGWGGPLIIEPKPGRDLIYSVTGGGIHPLAQHIANLTGGRPFDGFKSKADFSEIAVAVIDCGGTA
RIGVYPMKKVPTVDIYPTSPSGPLMRFITEEYFVSGVRPEDVELIDE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3732

COG function: function code G; Phosphotransferase system sorbitol-specific component IIBC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI48994905, Length=120, Percent_Identity=44.1666666666667, Blast_Score=110, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011638
- InterPro:   IPR011618
- InterPro:   IPR004702 [H]

Pfam domain/function: PF07663 EIIBC-GUT_C; PF03612 EIIBC-GUT_N [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 13973; Mature: 13842

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS51102 PTS_EIIB_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVKKYRKVRIKKGPKGWGGPLIIEPKPGRDLIYSVTGGGIHPLAQHIANLTGGRPFDG
CCCHHCHHEEEECCCCCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC
FKSKADFSEIAVAVIDCGGTARIGVYPMKKVPTVDIYPTSPSGPLMRFITEEYFVSGVRP
CCCCCCHHHEEEEEEECCCCEEECEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
EDVELIDE
CCCEECCC
>Mature Secondary Structure 
TEVKKYRKVRIKKGPKGWGGPLIIEPKPGRDLIYSVTGGGIHPLAQHIANLTGGRPFDG
CCHHCHHEEEECCCCCCCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCC
FKSKADFSEIAVAVIDCGGTARIGVYPMKKVPTVDIYPTSPSGPLMRFITEEYFVSGVRP
CCCCCCHHHEEEEEEECCCCEEECEEECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCC
EDVELIDE
CCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9572925 [H]