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The map label for this gene is proS

Identifier: 222524704

GI number: 222524704

Start: 1817814

End: 1819256

Strand: Reverse

Name: proS

Synonym: Chy400_1431

Alternate gene names: 222524704

Gene position: 1819256-1817814 (Counterclockwise)

Preceding gene: 222524705

Following gene: 222524703

Centisome position: 34.53

GC content: 55.72

Gene sequence:

>1443_bases
ATGCCAAAAGAGGTTATTACCCAACGGAGTGTTGATTACAACCAGTGGTACCTCGATATTGTGCGGGAAGCTGATCTGGC
CGAGGTTGCCGAAGTTGTCCGTGGTTGTATCGTCGTTAAGGCCCACGGCTGGGCGATCTGGGAATTGATGCAGCGTGCAC
TCGATGATCGGATCAAGGCAACCGGTCATGCCAACGTGCAGTTCCCTCTGCTCATCCCCAAGAGCTTTATTATGAAAGAG
GCCGAGCACGTCGAGGGCTTTGCTCCAGAAGTCGCAGAAGTCACCCGCGCCGGCGGTGAAGAACTGGCCGAGCCATATAT
TATCCGTCCTACCAGCGAGACCATCATCGGTTATTTCTACAGCAAGTGGATTCGCTCGTACCGCGATCTGCCTTTGCTCT
ACAACCAGTGGGCCAACGTCATGCGCTGGGAAATGCGCACTCGTCCTTTCCTGCGCACGGCTGAATTCTGGTGGCAGGAA
GGTCATACTGCGCATGCCACCGAAGCCGAGGCCGAGGAAGAGACCCTGCGCATTCTTCACGACGTTTATGCCGATTTCGT
TGAAAAAGAGATGGCCGTGCCGGTCATTCGCGGGCTTAAGACTGAAAAAGAGAAATTTCCCGGTGCGCTACGTTCATACT
GTATTGAGGCAATGATGCAGGATGGCCGGGCCTTGCAGGCCGGTACGTCGCATAATCTTGGTCAGAATTTTGCCCGCGCC
TTCGATATTACCTTTACCGATCAGAACAACACCATTCAGTACGCCTGGACGACCAGTTGGGGGGTAAGTACCCGTCTGAT
CGGTGCGCTGATTATGACCCATTCGGATGATGAGGGGCTGGTTTTGCCGCCTCGGCTGGCTCCCATTCAGGTGGTGGTAG
TGCCGATTTACAAGAACGACGAAGAGCGCAGCCTGGTGATGGCCGCCGTTGAGCAGATGACCGCAGCCTGGAAGGGTCGC
CTGCGCTTCAAAGTTGATGACCGCGACAATTACAGTCCCGGTTATAAATTCAACGAATGGGAGTTGAAGGGTGTTCCGGT
TCGGATTGAAGTTGGGCCGAAAGATGTGGCAAAAGAGACGGTGGCTCTCGCCCGCCGGGATATTCCCGGTAAAGCCGGCA
AGAGCTTCGTCCCCCAGGCCGGTCTGACCGAGCGGATTGAGGCATTGTTGAACGAGATGCAGACCGCACTCTTCCAGCGT
GCACTTGCTTTCCGCGAAGCGCACACTGCGGATGTCACCAGCTACGACGAATTGAAAGAGCAGATCGAACGAGGCTTTGC
CCGGGCCTACTGGGCCGGTGATACCGCTGATGAAAAGCGCATTCAAGAAGAGACGCGGGCGACAATCCGTTGCATTCCGC
TCGAGCAGCCGGGGTCGGTCGGTCGCTGTGTTTACACCGGTCGCGAAACGGATCGTCAGGTAATCTTTGCCCGTGCCTAT
TGA

Upstream 100 bases:

>100_bases
AAAACCGCTTTGCAATGAGTTTTGCCATATCAGCTATAATAGAGGGAGTATGAATAGAATGTAACGCCTACAGCATATCA
TCATCAGCCCGAGGAACGGC

Downstream 100 bases:

>100_bases
TGCGGTATCTGTTTAGTGTCGGAGAATACCATGAGCGCCATCCTGAGCAGCCTGGCTGCATTCATTCGTGAACGGTTACC
GCAGACAGGTGCGCGTTTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPKEVITQRSVDYNQWYLDIVREADLAEVAEVVRGCIVVKAHGWAIWELMQRALDDRIKATGHANVQFPLLIPKSFIMKE
AEHVEGFAPEVAEVTRAGGEELAEPYIIRPTSETIIGYFYSKWIRSYRDLPLLYNQWANVMRWEMRTRPFLRTAEFWWQE
GHTAHATEAEAEEETLRILHDVYADFVEKEMAVPVIRGLKTEKEKFPGALRSYCIEAMMQDGRALQAGTSHNLGQNFARA
FDITFTDQNNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLVLPPRLAPIQVVVVPIYKNDEERSLVMAAVEQMTAAWKGR
LRFKVDDRDNYSPGYKFNEWELKGVPVRIEVGPKDVAKETVALARRDIPGKAGKSFVPQAGLTERIEALLNEMQTALFQR
ALAFREAHTADVTSYDELKEQIERGFARAYWAGDTADEKRIQEETRATIRCIPLEQPGSVGRCVYTGRETDRQVIFARAY

Sequences:

>Translated_480_residues
MPKEVITQRSVDYNQWYLDIVREADLAEVAEVVRGCIVVKAHGWAIWELMQRALDDRIKATGHANVQFPLLIPKSFIMKE
AEHVEGFAPEVAEVTRAGGEELAEPYIIRPTSETIIGYFYSKWIRSYRDLPLLYNQWANVMRWEMRTRPFLRTAEFWWQE
GHTAHATEAEAEEETLRILHDVYADFVEKEMAVPVIRGLKTEKEKFPGALRSYCIEAMMQDGRALQAGTSHNLGQNFARA
FDITFTDQNNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLVLPPRLAPIQVVVVPIYKNDEERSLVMAAVEQMTAAWKGR
LRFKVDDRDNYSPGYKFNEWELKGVPVRIEVGPKDVAKETVALARRDIPGKAGKSFVPQAGLTERIEALLNEMQTALFQR
ALAFREAHTADVTSYDELKEQIERGFARAYWAGDTADEKRIQEETRATIRCIPLEQPGSVGRCVYTGRETDRQVIFARAY
>Mature_479_residues
PKEVITQRSVDYNQWYLDIVREADLAEVAEVVRGCIVVKAHGWAIWELMQRALDDRIKATGHANVQFPLLIPKSFIMKEA
EHVEGFAPEVAEVTRAGGEELAEPYIIRPTSETIIGYFYSKWIRSYRDLPLLYNQWANVMRWEMRTRPFLRTAEFWWQEG
HTAHATEAEAEEETLRILHDVYADFVEKEMAVPVIRGLKTEKEKFPGALRSYCIEAMMQDGRALQAGTSHNLGQNFARAF
DITFTDQNNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLVLPPRLAPIQVVVVPIYKNDEERSLVMAAVEQMTAAWKGRL
RFKVDDRDNYSPGYKFNEWELKGVPVRIEVGPKDVAKETVALARRDIPGKAGKSFVPQAGLTERIEALLNEMQTALFQRA
LAFREAHTADVTSYDELKEQIERGFARAYWAGDTADEKRIQEETRATIRCIPLEQPGSVGRCVYTGRETDRQVIFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=493, Percent_Identity=39.9594320486815, Blast_Score=360, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71991342, Length=496, Percent_Identity=40.5241935483871, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71991346, Length=359, Percent_Identity=47.075208913649, Blast_Score=342, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6321808, Length=507, Percent_Identity=40.0394477317554, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24649468, Length=492, Percent_Identity=39.0243902439024, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24649466, Length=492, Percent_Identity=39.0243902439024, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_CHLAA (A9W9Q2)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001634927.1
- ProteinModelPortal:   A9W9Q2
- SMR:   A9W9Q2
- GeneID:   5828414
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_1310
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 54886; Mature: 54755

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKEVITQRSVDYNQWYLDIVREADLAEVAEVVRGCIVVKAHGWAIWELMQRALDDRIKA
CCHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHC
TGHANVQFPLLIPKSFIMKEAEHVEGFAPEVAEVTRAGGEELAEPYIIRPTSETIIGYFY
CCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCEEECCCHHHHHHHHH
SKWIRSYRDLPLLYNQWANVMRWEMRTRPFLRTAEFWWQEGHTAHATEAEAEEETLRILH
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVYADFVEKEMAVPVIRGLKTEKEKFPGALRSYCIEAMMQDGRALQAGTSHNLGQNFARA
HHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHE
FDITFTDQNNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLVLPPRLAPIQVVVVPIYKND
EEEEEECCCCEEEEEEECCCCHHHHHHHHHHEEECCCCCEEECCCCCCEEEEEEEEECCC
EERSLVMAAVEQMTAAWKGRLRFKVDDRDNYSPGYKFNEWELKGVPVRIEVGPKDVAKET
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEECCHHHHHHH
VALARRDIPGKAGKSFVPQAGLTERIEALLNEMQTALFQRALAFREAHTADVTSYDELKE
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
QIERGFARAYWAGDTADEKRIQEETRATIRCIPLEQPGSVGRCVYTGRETDRQVIFARAY
HHHHHHHHEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCEEEEECCCCCCEEEEEECC
>Mature Secondary Structure 
PKEVITQRSVDYNQWYLDIVREADLAEVAEVVRGCIVVKAHGWAIWELMQRALDDRIKA
CHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHC
TGHANVQFPLLIPKSFIMKEAEHVEGFAPEVAEVTRAGGEELAEPYIIRPTSETIIGYFY
CCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHCCCEEECCCHHHHHHHHH
SKWIRSYRDLPLLYNQWANVMRWEMRTRPFLRTAEFWWQEGHTAHATEAEAEEETLRILH
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DVYADFVEKEMAVPVIRGLKTEKEKFPGALRSYCIEAMMQDGRALQAGTSHNLGQNFARA
HHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHE
FDITFTDQNNTIQYAWTTSWGVSTRLIGALIMTHSDDEGLVLPPRLAPIQVVVVPIYKND
EEEEEECCCCEEEEEEECCCCHHHHHHHHHHEEECCCCCEEECCCCCCEEEEEEEEECCC
EERSLVMAAVEQMTAAWKGRLRFKVDDRDNYSPGYKFNEWELKGVPVRIEVGPKDVAKET
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEECCHHHHHHH
VALARRDIPGKAGKSFVPQAGLTERIEALLNEMQTALFQRALAFREAHTADVTSYDELKE
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
QIERGFARAYWAGDTADEKRIQEETRATIRCIPLEQPGSVGRCVYTGRETDRQVIFARAY
HHHHHHHHEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCCEEEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA