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The map label for this gene is pcrA [H]

Identifier: 222524699

GI number: 222524699

Start: 1809841

End: 1812081

Strand: Reverse

Name: pcrA [H]

Synonym: Chy400_1426

Alternate gene names: 222524699

Gene position: 1812081-1809841 (Counterclockwise)

Preceding gene: 222524700

Following gene: 222524697

Centisome position: 34.39

GC content: 54.84

Gene sequence:

>2241_bases
ATGACAGGTGATCTTTTAGCTAACCTCAATGAACCCCAGCAACGTGCCGTGACCACCGTTACCGGGCCGGTGCTGGTATT
GGCCGGGCCGGGAAGTGGTAAGACTCGTGTGCTTACCCACCGGATCGCCTATCTGATCGCCGAGACGGGGATTGATCCGC
TTCAGATACTGGCAGTCACCTTCACCAACAAGGCAGCGCGTGAGATTCGCGAACGTCTGAACCTGCTCGTGGGCGAAACC
GTTGCTCATGAGATAACCATCGGTACCTTTCATGCGATTTGTACGCGCTGGCTGCGACGCGATATTGTTCATCTCGGTCG
TGAACGTGACTTCGTGATCTACGATAGCGATGATCAAGAACGCCTGATGAAGCGGGTGTTGCGCGAACTGAATCTCAATG
AAAAGCAGTATCCGCCGCGCGCTATTCTAGGGGTCATTTCACGAGCCAAAAATGAGCTGGTTGATCCAGAGGCATTTGCT
CGCACTGCCCGTAGCTACTTCGACGAGATTGTCGCCCGTTGCTACACACGGTACCAACAGCTCCTGATTGAAAGCAACGC
GCTCGACTTCGATGATCTGCTCGTCGAAACGGTGCGCCTGTTCCAGGAACATCCTGCCATCCTTCATCATTACCATCAGC
GCTACCGGTTCCTGCTCGTCGATGAGTATCAGGATACGAACCGTGCGCAGTATCTGATCGTGCGTGCTCTTGCCGAACGC
GACCGCAATCTGTTTGTGGTCGGGGATGAAGCACAGAGTATCTATGCCTGGCGTGGCGCCGATATTCGTAATATCTTGCA
ATTTGAACACGATTATCCCGATGCCAGGGTGATTTTGCTCGAACAGAATTATCGCAGCACGCAGGCTATTCTCGATGCTG
CGCAGGCGTTGATGCACGCCAGCCCCCAACGCAAATACGCCAAAAACCTCTGGACGGTCAACGGCAAAGGCGAGGCGGTC
ACGCTGTACGAGGCAAGCGATGAAGAGGACGAAGTCCAGTTTGTCGTTGATGAAATTCTGCGCTTAATCGCGGAAGGAAA
GGCTCGTCCCGGCGATTGCGCCATCATGTACCGTACCAATGCGCAGTCCCGTCTGGTTGAAGAGGCTCTCATTCATGCCG
GTGTGCCGTACTACGTGGTTGGTGGGGTGCGTTTCTACGAACGCAAAGAGATCAAAGATGTGCTCGCGTATCTCCGGCTG
ATAGCAAATCCATACGATAGCGTCAGTCTACAACGCATCATCAACTGGCCAGGTCGTGGGATTGGCGAGCGTACCGTTGC
GGAGCTGATCGCCTGGGCCAGGACGTTGGGACTACCACTCTATCAGGCGTTGCGCGAGATTGCCGACAATGATGCGATTT
CCCATCCCTTTGGTGGACGAGCGCGTACTGCACTGACTGAGTTTTATCGCCTGCTGAGCGAGCTGCATCAGTTGCACGGA
ATGATGGCGCTGAGTGATCTGATCGATTATCTGCTCAATCGAATCGACGTGCGCGCAACCCTCTACAGCGAATACGATGG
CGATGAAGCCGATGATCGCTGGCGCAACGTGCAGGAGTTACGAAATGCGGCCTTGAAGTATGTCAACTTGCCGGTTGAGA
CTCAACTTACTGCCTTTCTCGAAGAGATTGCTCTGGTTGCCGATGTTGATGCGATTGATCGCAACAGCGATACGGTCACC
TGCATCACACTCCATCAGGCGAAAGGGCTTGAGTTTCCCTGTGTCTTTCTGCTAGGGATTGAAGAGGGACTGCTGCCGCA
TAGCCGGTCGCTGGATAACCGCGACTCGATTGAAGAAGAGCGCCGTCTCCTTTACGTCGGGATGACCCGTGCCCAACGGC
GTTTATACCTCTGTCATGCGACAAAACGCACCATGTTCGGTCGCGTGAATATCGGTGCCCGCTCTCGGTTTCTCAACGAC
ATTCCACCGACACTCGTCCAGCAGGTGCATCGTCGTGGTTATCGGGCTATTCCCGCTGTAGCTGGGACAACGTGGCAGTT
CCGCACCTCTGGTCAGTCTGAGCAGAACCGACGCACGTCAAGAAACCAGGCTCCGCTTCCCACACCACAAAAAGCCGTTT
TCTTCCCTGGACAGCGCGTGCGACACGCCACCTTCGGCGAAGGAATTGTCGTTGACAGCCGCCTGATCGAAGGTGATGAA
GAGGTGACCGTCCGTTTTGCTGATCGCGAACGCCGACTATTGGCGAGTTTTGCCCGGCTTGAACAGCTTAGGGACGGATA
A

Upstream 100 bases:

>100_bases
GTTGATATCATCATCAAACAACCCACCTGCCAGCAAAGCAGGTGTGCTATACTGTTCGTGCCTGAATTGTCAGAACGTAT
TACCAATTCCCAAATCGATC

Downstream 100 bases:

>100_bases
AGGTCTCAATTGGCCGCTGGCGCACTTCGTTCACCAGAAACTCGCGCAAGGCACGGGCTGCCGGCGAAGCTGTGCGATCA
CTCCGCCAGACCAGGCCGAG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MTGDLLANLNEPQQRAVTTVTGPVLVLAGPGSGKTRVLTHRIAYLIAETGIDPLQILAVTFTNKAAREIRERLNLLVGET
VAHEITIGTFHAICTRWLRRDIVHLGRERDFVIYDSDDQERLMKRVLRELNLNEKQYPPRAILGVISRAKNELVDPEAFA
RTARSYFDEIVARCYTRYQQLLIESNALDFDDLLVETVRLFQEHPAILHHYHQRYRFLLVDEYQDTNRAQYLIVRALAER
DRNLFVVGDEAQSIYAWRGADIRNILQFEHDYPDARVILLEQNYRSTQAILDAAQALMHASPQRKYAKNLWTVNGKGEAV
TLYEASDEEDEVQFVVDEILRLIAEGKARPGDCAIMYRTNAQSRLVEEALIHAGVPYYVVGGVRFYERKEIKDVLAYLRL
IANPYDSVSLQRIINWPGRGIGERTVAELIAWARTLGLPLYQALREIADNDAISHPFGGRARTALTEFYRLLSELHQLHG
MMALSDLIDYLLNRIDVRATLYSEYDGDEADDRWRNVQELRNAALKYVNLPVETQLTAFLEEIALVADVDAIDRNSDTVT
CITLHQAKGLEFPCVFLLGIEEGLLPHSRSLDNRDSIEEERRLLYVGMTRAQRRLYLCHATKRTMFGRVNIGARSRFLND
IPPTLVQQVHRRGYRAIPAVAGTTWQFRTSGQSEQNRRTSRNQAPLPTPQKAVFFPGQRVRHATFGEGIVVDSRLIEGDE
EVTVRFADRERRLLASFARLEQLRDG

Sequences:

>Translated_746_residues
MTGDLLANLNEPQQRAVTTVTGPVLVLAGPGSGKTRVLTHRIAYLIAETGIDPLQILAVTFTNKAAREIRERLNLLVGET
VAHEITIGTFHAICTRWLRRDIVHLGRERDFVIYDSDDQERLMKRVLRELNLNEKQYPPRAILGVISRAKNELVDPEAFA
RTARSYFDEIVARCYTRYQQLLIESNALDFDDLLVETVRLFQEHPAILHHYHQRYRFLLVDEYQDTNRAQYLIVRALAER
DRNLFVVGDEAQSIYAWRGADIRNILQFEHDYPDARVILLEQNYRSTQAILDAAQALMHASPQRKYAKNLWTVNGKGEAV
TLYEASDEEDEVQFVVDEILRLIAEGKARPGDCAIMYRTNAQSRLVEEALIHAGVPYYVVGGVRFYERKEIKDVLAYLRL
IANPYDSVSLQRIINWPGRGIGERTVAELIAWARTLGLPLYQALREIADNDAISHPFGGRARTALTEFYRLLSELHQLHG
MMALSDLIDYLLNRIDVRATLYSEYDGDEADDRWRNVQELRNAALKYVNLPVETQLTAFLEEIALVADVDAIDRNSDTVT
CITLHQAKGLEFPCVFLLGIEEGLLPHSRSLDNRDSIEEERRLLYVGMTRAQRRLYLCHATKRTMFGRVNIGARSRFLND
IPPTLVQQVHRRGYRAIPAVAGTTWQFRTSGQSEQNRRTSRNQAPLPTPQKAVFFPGQRVRHATFGEGIVVDSRLIEGDE
EVTVRFADRERRLLASFARLEQLRDG
>Mature_745_residues
TGDLLANLNEPQQRAVTTVTGPVLVLAGPGSGKTRVLTHRIAYLIAETGIDPLQILAVTFTNKAAREIRERLNLLVGETV
AHEITIGTFHAICTRWLRRDIVHLGRERDFVIYDSDDQERLMKRVLRELNLNEKQYPPRAILGVISRAKNELVDPEAFAR
TARSYFDEIVARCYTRYQQLLIESNALDFDDLLVETVRLFQEHPAILHHYHQRYRFLLVDEYQDTNRAQYLIVRALAERD
RNLFVVGDEAQSIYAWRGADIRNILQFEHDYPDARVILLEQNYRSTQAILDAAQALMHASPQRKYAKNLWTVNGKGEAVT
LYEASDEEDEVQFVVDEILRLIAEGKARPGDCAIMYRTNAQSRLVEEALIHAGVPYYVVGGVRFYERKEIKDVLAYLRLI
ANPYDSVSLQRIINWPGRGIGERTVAELIAWARTLGLPLYQALREIADNDAISHPFGGRARTALTEFYRLLSELHQLHGM
MALSDLIDYLLNRIDVRATLYSEYDGDEADDRWRNVQELRNAALKYVNLPVETQLTAFLEEIALVADVDAIDRNSDTVTC
ITLHQAKGLEFPCVFLLGIEEGLLPHSRSLDNRDSIEEERRLLYVGMTRAQRRLYLCHATKRTMFGRVNIGARSRFLNDI
PPTLVQQVHRRGYRAIPAVAGTTWQFRTSGQSEQNRRTSRNQAPLPTPQKAVFFPGQRVRHATFGEGIVVDSRLIEGDEE
VTVRFADRERRLLASFARLEQLRDG

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=746, Percent_Identity=41.2868632707775, Blast_Score=504, Evalue=1e-143,
Organism=Escherichia coli, GI48994965, Length=643, Percent_Identity=38.8802488335925, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6322369, Length=764, Percent_Identity=30.1047120418848, Blast_Score=231, Evalue=3e-61,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85405; Mature: 85274

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDLLANLNEPQQRAVTTVTGPVLVLAGPGSGKTRVLTHRIAYLIAETGIDPLQILAVT
CCCCHHCCCCCHHHHHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEH
FTNKAAREIRERLNLLVGETVAHEITIGTFHAICTRWLRRDIVHLGRERDFVIYDSDDQE
HCCHHHHHHHHHHHHHHCCHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
RLMKRVLRELNLNEKQYPPRAILGVISRAKNELVDPEAFARTARSYFDEIVARCYTRYQQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLIESNALDFDDLLVETVRLFQEHPAILHHYHQRYRFLLVDEYQDTNRAQYLIVRALAER
HHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHC
DRNLFVVGDEAQSIYAWRGADIRNILQFEHDYPDARVILLEQNYRSTQAILDAAQALMHA
CCCEEEEECCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHC
SPQRKYAKNLWTVNGKGEAVTLYEASDEEDEVQFVVDEILRLIAEGKARPGDCAIMYRTN
CCHHHHHHHCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
AQSRLVEEALIHAGVPYYVVGGVRFYERKEIKDVLAYLRLIANPYDSVSLQRIINWPGRG
CHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
IGERTVAELIAWARTLGLPLYQALREIADNDAISHPFGGRARTALTEFYRLLSELHQLHG
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MMALSDLIDYLLNRIDVRATLYSEYDGDEADDRWRNVQELRNAALKYVNLPVETQLTAFL
HHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EEIALVADVDAIDRNSDTVTCITLHQAKGLEFPCVFLLGIEEGLLPHSRSLDNRDSIEEE
HHHHHHHCCHHCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
RRLLYVGMTRAQRRLYLCHATKRTMFGRVNIGARSRFLNDIPPTLVQQVHRRGYRAIPAV
HHEEEEEHHHHHHEEEEEEHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHCCCEECCCC
AGTTWQFRTSGQSEQNRRTSRNQAPLPTPQKAVFFPGQRVRHATFGEGIVVDSRLIEGDE
CCCCEEEECCCCCHHHHHHCCCCCCCCCCCCEEECCCCHHCCCCCCCCEEEECCEECCCC
EVTVRFADRERRLLASFARLEQLRDG
EEEEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGDLLANLNEPQQRAVTTVTGPVLVLAGPGSGKTRVLTHRIAYLIAETGIDPLQILAVT
CCCHHCCCCCHHHHHHEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHEEEEH
FTNKAAREIRERLNLLVGETVAHEITIGTFHAICTRWLRRDIVHLGRERDFVIYDSDDQE
HCCHHHHHHHHHHHHHHCCHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHH
RLMKRVLRELNLNEKQYPPRAILGVISRAKNELVDPEAFARTARSYFDEIVARCYTRYQQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLIESNALDFDDLLVETVRLFQEHPAILHHYHQRYRFLLVDEYQDTNRAQYLIVRALAER
HHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHC
DRNLFVVGDEAQSIYAWRGADIRNILQFEHDYPDARVILLEQNYRSTQAILDAAQALMHA
CCCEEEEECCCCEEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHC
SPQRKYAKNLWTVNGKGEAVTLYEASDEEDEVQFVVDEILRLIAEGKARPGDCAIMYRTN
CCHHHHHHHCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
AQSRLVEEALIHAGVPYYVVGGVRFYERKEIKDVLAYLRLIANPYDSVSLQRIINWPGRG
CHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCC
IGERTVAELIAWARTLGLPLYQALREIADNDAISHPFGGRARTALTEFYRLLSELHQLHG
CCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
MMALSDLIDYLLNRIDVRATLYSEYDGDEADDRWRNVQELRNAALKYVNLPVETQLTAFL
HHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EEIALVADVDAIDRNSDTVTCITLHQAKGLEFPCVFLLGIEEGLLPHSRSLDNRDSIEEE
HHHHHHHCCHHCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHH
RRLLYVGMTRAQRRLYLCHATKRTMFGRVNIGARSRFLNDIPPTLVQQVHRRGYRAIPAV
HHEEEEEHHHHHHEEEEEEHHHHHHEEEECCCHHHHHHHCCCHHHHHHHHHCCCEECCCC
AGTTWQFRTSGQSEQNRRTSRNQAPLPTPQKAVFFPGQRVRHATFGEGIVVDSRLIEGDE
CCCCEEEECCCCCHHHHHHCCCCCCCCCCCCEEECCCCHHCCCCCCCCEEEECCEECCCC
EVTVRFADRERRLLASFARLEQLRDG
EEEEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]