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The map label for this gene is galE [C]

Identifier: 222524616

GI number: 222524616

Start: 1713526

End: 1714509

Strand: Reverse

Name: galE [C]

Synonym: Chy400_1340

Alternate gene names: 222524616

Gene position: 1714509-1713526 (Counterclockwise)

Preceding gene: 222524617

Following gene: 222524615

Centisome position: 32.54

GC content: 57.72

Gene sequence:

>984_bases
ATGAAGCGGGTGCTTATCAACGGCGCAAAAGGAATGTTAAGTGCGCAGGCCGCCCAGGTGTTGAGCCAGCATCACGATGT
TATCGTCCTGGGACAACAACCACCAACAGCTCCTATCGGCAAAGCCGACTGGCTGGTAGCCCGACTTGATCGTCATCAGT
TGCTTGAGTTATTGCGCCATGAGCAGATAGACACCGTCGTTCATTGCGATCTGTTGGGGTTCGATGAACCGCTGCCTGAC
CACGAAACGACGGTTCAGCACAATGTCATTGGCACGATGGAGTTGCTCGGTGCCTGTCGTGCTGCCGGCATACGCCATGT
TGTGTTACGCAGTCATGGCTGGATCTATGGCGCCAGTCCGTTGAATCCGTTGTTGATCAGCGAAGAGCGTCCGATCAAGA
CGCAGCATAGCCGGGGCCTCCTGCGTACTCTGGGTGAAGTAGAGCAGGTGGTGGCCGATTTTGCTTCCCGCCACCCTCAA
ATCACAGTGACGGTGTTGCGCTATGCACCGCTGCTTGACCAGGATAGCCCGCTGATGCGCTATCTGCGCGCACCAGCCCC
CCAGATGTTGTTCGGCTTCGACCCAATGATCCAGCTACTCCACCTCACCGATGCTGCTCAGGCAGTGTTGGCGGCGGTGC
AGCAACCGATTGGCGGTGCTTTCAATCTCGCTCCAGCGCAATCGATGCCGCTGAGCCAGGTCATTCGCCGGCTGGGACAT
CAGCCGGTACCGGTATTAGGCCCTCTCTTCGACAATCAACCGCCGGCCGGATGGCCCTTCGACGTTGATTTCTTGCGCTA
CCGTTGTACTATCGATCCTGAGCGTGCCTGTCGCTTGCTAGGATGGTCGGCCCACTACGATATGGCAACGGCTCTCGACA
CACTGAAGCCGTTGTCGCCAGAAGAGGAGCGGGCTACGGCTGCCCGCGCCCTCGATGAATTTTTCAATCGCAGGAGAATG
CACGATGAACGACGAGCCGGTTGA

Upstream 100 bases:

>100_bases
GACCTGATGAGATTGCCTGGGTGTTTCGGGCATTGAGCCACGGTGATATGGATCGGGAAGAGCTTGAGCGCTGGTTGCGC
GAAAATGTAGGAATATTGCC

Downstream 100 bases:

>100_bases
TCCACTGACCAGTGATACAGAGGTTCAGCATCCCCGGCGACGCCGGACAAAGGCGGTGACCATTGAGACAACCTCAGAGA
CGCCGACAACCGCCGATCCC

Product: NAD-dependent epimerase/dehydratase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKRVLINGAKGMLSAQAAQVLSQHHDVIVLGQQPPTAPIGKADWLVARLDRHQLLELLRHEQIDTVVHCDLLGFDEPLPD
HETTVQHNVIGTMELLGACRAAGIRHVVLRSHGWIYGASPLNPLLISEERPIKTQHSRGLLRTLGEVEQVVADFASRHPQ
ITVTVLRYAPLLDQDSPLMRYLRAPAPQMLFGFDPMIQLLHLTDAAQAVLAAVQQPIGGAFNLAPAQSMPLSQVIRRLGH
QPVPVLGPLFDNQPPAGWPFDVDFLRYRCTIDPERACRLLGWSAHYDMATALDTLKPLSPEEERATAARALDEFFNRRRM
HDERRAG

Sequences:

>Translated_327_residues
MKRVLINGAKGMLSAQAAQVLSQHHDVIVLGQQPPTAPIGKADWLVARLDRHQLLELLRHEQIDTVVHCDLLGFDEPLPD
HETTVQHNVIGTMELLGACRAAGIRHVVLRSHGWIYGASPLNPLLISEERPIKTQHSRGLLRTLGEVEQVVADFASRHPQ
ITVTVLRYAPLLDQDSPLMRYLRAPAPQMLFGFDPMIQLLHLTDAAQAVLAAVQQPIGGAFNLAPAQSMPLSQVIRRLGH
QPVPVLGPLFDNQPPAGWPFDVDFLRYRCTIDPERACRLLGWSAHYDMATALDTLKPLSPEEERATAARALDEFFNRRRM
HDERRAG
>Mature_327_residues
MKRVLINGAKGMLSAQAAQVLSQHHDVIVLGQQPPTAPIGKADWLVARLDRHQLLELLRHEQIDTVVHCDLLGFDEPLPD
HETTVQHNVIGTMELLGACRAAGIRHVVLRSHGWIYGASPLNPLLISEERPIKTQHSRGLLRTLGEVEQVVADFASRHPQ
ITVTVLRYAPLLDQDSPLMRYLRAPAPQMLFGFDPMIQLLHLTDAAQAVLAAVQQPIGGAFNLAPAQSMPLSQVIRRLGH
QPVPVLGPLFDNQPPAGWPFDVDFLRYRCTIDPERACRLLGWSAHYDMATALDTLKPLSPEEERATAARALDEFFNRRRM
HDERRAG

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786974, Length=180, Percent_Identity=30, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 36461; Mature: 36461

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVLINGAKGMLSAQAAQVLSQHHDVIVLGQQPPTAPIGKADWLVARLDRHQLLELLRH
CCCEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EQIDTVVHCDLLGFDEPLPDHETTVQHNVIGTMELLGACRAAGIRHVVLRSHGWIYGASP
HHHHHHEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCC
LNPLLISEERPIKTQHSRGLLRTLGEVEQVVADFASRHPQITVTVLRYAPLLDQDSPLMR
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCHHHH
YLRAPAPQMLFGFDPMIQLLHLTDAAQAVLAAVQQPIGGAFNLAPAQSMPLSQVIRRLGH
HHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHCC
QPVPVLGPLFDNQPPAGWPFDVDFLRYRCTIDPERACRLLGWSAHYDMATALDTLKPLSP
CCCCCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCC
EEERATAARALDEFFNRRRMHDERRAG
HHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MKRVLINGAKGMLSAQAAQVLSQHHDVIVLGQQPPTAPIGKADWLVARLDRHQLLELLRH
CCCEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EQIDTVVHCDLLGFDEPLPDHETTVQHNVIGTMELLGACRAAGIRHVVLRSHGWIYGASP
HHHHHHEEEHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECCC
LNPLLISEERPIKTQHSRGLLRTLGEVEQVVADFASRHPQITVTVLRYAPLLDQDSPLMR
CCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCHHHH
YLRAPAPQMLFGFDPMIQLLHLTDAAQAVLAAVQQPIGGAFNLAPAQSMPLSQVIRRLGH
HHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHCC
QPVPVLGPLFDNQPPAGWPFDVDFLRYRCTIDPERACRLLGWSAHYDMATALDTLKPLSP
CCCCCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCC
EEERATAARALDEFFNRRRMHDERRAG
HHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]