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The map label for this gene is llaDCHIA [H]

Identifier: 222524537

GI number: 222524537

Start: 1605760

End: 1606638

Strand: Reverse

Name: llaDCHIA [H]

Synonym: Chy400_1261

Alternate gene names: 222524537

Gene position: 1606638-1605760 (Counterclockwise)

Preceding gene: 222524538

Following gene: 222524534

Centisome position: 30.49

GC content: 54.95

Gene sequence:

>879_bases
GTGCCTGCGCGTCCATTCCTCAAGTGGGCTGGTGGGAAAAGTCAGCTCTTGCCAGAGCTTTCGCGGCGCATTCCAGAGCG
TTTTGGCCGCTATCACGAGCCGTTTGTCGGTGGTGGCGCACTCTTCTTTTACCTCTGGAACAATGGTCTGTTGCGACACG
GGGCCATGCTGAGCGACCTCAACGCGGAACTGATCGACTGTTACATTGCTGTTCGTGATGAGGTTGAGGATCTGATTGAA
CTGCTGCATCGTCTGCGCCCGTATGCGACCGACCGTGATTTCTTCTACGATATTCGCAGTTGGGATCGCCAACCAGATTT
TGCTCGTCGCTCACGGGTGGAACGTGCAGCCCGCACCATCTTTCTCAATCGCACCTGCTACAATGGTCTCTATCGCCTGA
ACAATAAGGGGCAGTTCAACGCCCCCTTCGGCTATTACAAGAATCCGCAGATTGTAGACAGCGATAATCTCCGTGAGGTG
AGTCGGGCCTTACGCAATGTTGATCTGCGGGTGGCCGATTTCGCGGCTGTCCTCGATGAGGCTCAGGCGGGTGATTTTGT
CTACTTCGATCCGCCGTATGTGCCGGTAAGCAGTACCGCTTCCTTTACCAGTTACACACGACGCGGCTTCGATGAGGGTG
AACAGCGTCGGCTTGCCCTGGTCTTTCATCAACTGGCAGCGCGGAATTGTTTCGTGATGCTCTCGAATTCATCAACAACC
CTGGCACATCAGTTGTACGCCAACGCCTGCCGGGTCGATGTTGTGCTGGCCAGTCGTAAAATCAATTGCAACGGCTCACG
TCGCGGGCTGGTTGAAGAGCTGATTGCCTGTAGTTTTCCGGCTGGCGTGTTCACTCCACACAAGCTTGCCAGTGATTAG

Upstream 100 bases:

>100_bases
GCACATAGCTCTTCTATGCGATATGTTATAATCCAATTCTCAATAACCGCATATGGGAGTCGAACCTGCCTGGTCTGGCA
GGTTTGGCGAGGAGCCTTGT

Downstream 100 bases:

>100_bases
AATGTGTGCCGGTACACGGCCTGTCAACGATATAGATGCGTCTACTCGCTCCGGCGCCAGTTCCAGGCGATCACGGCCAG
ATAACCTGCGGCACCAATTG

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPARPFLKWAGGKSQLLPELSRRIPERFGRYHEPFVGGGALFFYLWNNGLLRHGAMLSDLNAELIDCYIAVRDEVEDLIE
LLHRLRPYATDRDFFYDIRSWDRQPDFARRSRVERAARTIFLNRTCYNGLYRLNNKGQFNAPFGYYKNPQIVDSDNLREV
SRALRNVDLRVADFAAVLDEAQAGDFVYFDPPYVPVSSTASFTSYTRRGFDEGEQRRLALVFHQLAARNCFVMLSNSSTT
LAHQLYANACRVDVVLASRKINCNGSRRGLVEELIACSFPAGVFTPHKLASD

Sequences:

>Translated_292_residues
MPARPFLKWAGGKSQLLPELSRRIPERFGRYHEPFVGGGALFFYLWNNGLLRHGAMLSDLNAELIDCYIAVRDEVEDLIE
LLHRLRPYATDRDFFYDIRSWDRQPDFARRSRVERAARTIFLNRTCYNGLYRLNNKGQFNAPFGYYKNPQIVDSDNLREV
SRALRNVDLRVADFAAVLDEAQAGDFVYFDPPYVPVSSTASFTSYTRRGFDEGEQRRLALVFHQLAARNCFVMLSNSSTT
LAHQLYANACRVDVVLASRKINCNGSRRGLVEELIACSFPAGVFTPHKLASD
>Mature_291_residues
PARPFLKWAGGKSQLLPELSRRIPERFGRYHEPFVGGGALFFYLWNNGLLRHGAMLSDLNAELIDCYIAVRDEVEDLIEL
LHRLRPYATDRDFFYDIRSWDRQPDFARRSRVERAARTIFLNRTCYNGLYRLNNKGQFNAPFGYYKNPQIVDSDNLREVS
RALRNVDLRVADFAAVLDEAQAGDFVYFDPPYVPVSSTASFTSYTRRGFDEGEQRRLALVFHQLAARNCFVMLSNSSTTL
AHQLYANACRVDVVLASRKINCNGSRRGLVEELIACSFPAGVFTPHKLASD

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=288, Percent_Identity=35.4166666666667, Blast_Score=147, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 33409; Mature: 33278

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPARPFLKWAGGKSQLLPELSRRIPERFGRYHEPFVGGGALFFYLWNNGLLRHGAMLSDL
CCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCHHHHC
NAELIDCYIAVRDEVEDLIELLHRLRPYATDRDFFYDIRSWDRQPDFARRSRVERAARTI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FLNRTCYNGLYRLNNKGQFNAPFGYYKNPQIVDSDNLREVSRALRNVDLRVADFAAVLDE
HCCCHHHHHHEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
AQAGDFVYFDPPYVPVSSTASFTSYTRRGFDEGEQRRLALVFHQLAARNCFVMLSNSSTT
HCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHH
LAHQLYANACRVDVVLASRKINCNGSRRGLVEELIACSFPAGVFTPHKLASD
HHHHHHHHHHHEEEEEECEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHCCC
>Mature Secondary Structure 
PARPFLKWAGGKSQLLPELSRRIPERFGRYHEPFVGGGALFFYLWNNGLLRHGAMLSDL
CCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHCCCHHHHC
NAELIDCYIAVRDEVEDLIELLHRLRPYATDRDFFYDIRSWDRQPDFARRSRVERAARTI
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
FLNRTCYNGLYRLNNKGQFNAPFGYYKNPQIVDSDNLREVSRALRNVDLRVADFAAVLDE
HCCCHHHHHHEEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCHHHHHHHHHHH
AQAGDFVYFDPPYVPVSSTASFTSYTRRGFDEGEQRRLALVFHQLAARNCFVMLSNSSTT
HCCCCEEEECCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHH
LAHQLYANACRVDVVLASRKINCNGSRRGLVEELIACSFPAGVFTPHKLASD
HHHHHHHHHHHEEEEEECEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]