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The map label for this gene is rsbW [H]

Identifier: 222524434

GI number: 222524434

Start: 1468989

End: 1469738

Strand: Reverse

Name: rsbW [H]

Synonym: Chy400_1158

Alternate gene names: 222524434

Gene position: 1469738-1468989 (Counterclockwise)

Preceding gene: 222524435

Following gene: 222524433

Centisome position: 27.89

GC content: 52.0

Gene sequence:

>750_bases
ATGGAGAGTATTACCCTTACTGCTGATCTGGATGCGTTGGCCAAAACCAGTGCATTTATCACCGCCGCAGCCGAGCGTTG
TGGTCTGGATGAACGGGCAACCTGGCAGGTGCAGCTTGCAGTTGATGAAGCTGTAACCAACGTCATCCAGCACGCTTACG
ATCCTGACCAACCCGGTGATTTCACCCTGAGCTGGCAGTGCCATGATCAACGCTTTATCGTGACTGTACGCGATCATGGT
CGTCAGTTTGATCCATCTACTGTGCCGGTACCTGACATCACCTCGCCGTTGGAAGAGCGTCAGGTTGGCGGCCTGGGTAT
CTATCTCATTACGCGCTTAATGGACGAAGTTCATTTTGAATTTTCACCCCAAGGCGGCAATTTACTAACGATGGTCAAGT
ATCTACCATCTGATCAGGCTGAAGAAGCCAATGAAGTGGTCGTTATTCCCATCAACGACCGGATCGATGCGCTGACCGCA
CCCCGACTGAGCAAGACGGTGAACGAACAGATCAGCCAGGGCGCTCGTCAGATTGTGTTGGACTTCAGCAATGTTCCTTT
TCTGTCCAGTAGTGGCTTGCGCGCGTTGCTTCTTATCCGTAAGGAGTTGATGACGCTCGGCGGTGAATTACGGTTGGCCG
CTTTACAACCGCAAGTCCACGAAGTCTTTACGATTACCGGCTTTACCCAGGTTTTCAATATTCATCCCTCAGTCACAGAA
GCCCGCGCAGCATTTTCCCAGAGGCGTTGA

Upstream 100 bases:

>100_bases
TCAGTTCGCTCTTCGAGATCTATGGCGATACCGTCGAAGCAGTTGGATCATTTTGAACCTGCCCTATTGCCAGACGTGTT
CGTCTGGTCGCACAATCGCC

Downstream 100 bases:

>100_bases
GTGTCGCGCGAACAGATGCTGACATGGTATGGGTTGGTGGTTGGCGTGGTGGCGTGGATGTGGCTGTTTACCCTGCCCGC
TACGCCGGTAGCTCCCATAC

Product: anti-sigma-factor antagonist

Products: NA

Alternate protein names: Anti-sigma-B factor; Sigma-B negative effector rsbW [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MESITLTADLDALAKTSAFITAAAERCGLDERATWQVQLAVDEAVTNVIQHAYDPDQPGDFTLSWQCHDQRFIVTVRDHG
RQFDPSTVPVPDITSPLEERQVGGLGIYLITRLMDEVHFEFSPQGGNLLTMVKYLPSDQAEEANEVVVIPINDRIDALTA
PRLSKTVNEQISQGARQIVLDFSNVPFLSSSGLRALLLIRKELMTLGGELRLAALQPQVHEVFTITGFTQVFNIHPSVTE
ARAAFSQRR

Sequences:

>Translated_249_residues
MESITLTADLDALAKTSAFITAAAERCGLDERATWQVQLAVDEAVTNVIQHAYDPDQPGDFTLSWQCHDQRFIVTVRDHG
RQFDPSTVPVPDITSPLEERQVGGLGIYLITRLMDEVHFEFSPQGGNLLTMVKYLPSDQAEEANEVVVIPINDRIDALTA
PRLSKTVNEQISQGARQIVLDFSNVPFLSSSGLRALLLIRKELMTLGGELRLAALQPQVHEVFTITGFTQVFNIHPSVTE
ARAAFSQRR
>Mature_249_residues
MESITLTADLDALAKTSAFITAAAERCGLDERATWQVQLAVDEAVTNVIQHAYDPDQPGDFTLSWQCHDQRFIVTVRDHG
RQFDPSTVPVPDITSPLEERQVGGLGIYLITRLMDEVHFEFSPQGGNLLTMVKYLPSDQAEEANEVVVIPINDRIDALTA
PRLSKTVNEQISQGARQIVLDFSNVPFLSSSGLRALLLIRKELMTLGGELRLAALQPQVHEVFTITGFTQVFNIHPSVTE
ARAAFSQRR

Specific function: Negative regulator of sigma-B activity. Phosphorylates and inactivates its specific antagonist protein, rsbV. Upon phosphorylation of rsbV, rsbW is released and binds to sigma-B, thereby blocking its ability to form an RNA polymerase holoenzyme (E-sigma-B

COG id: COG2172

COG function: function code T; Anti-sigma regulatory factor (Ser/Thr protein kinase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010193
- InterPro:   IPR003594 [H]

Pfam domain/function: PF02518 HATPase_c [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 27613; Mature: 27613

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50801 STAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESITLTADLDALAKTSAFITAAAERCGLDERATWQVQLAVDEAVTNVIQHAYDPDQPGD
CCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
FTLSWQCHDQRFIVTVRDHGRQFDPSTVPVPDITSPLEERQVGGLGIYLITRLMDEVHFE
EEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCEE
FSPQGGNLLTMVKYLPSDQAEEANEVVVIPINDRIDALTAPRLSKTVNEQISQGARQIVL
ECCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHEEEE
DFSNVPFLSSSGLRALLLIRKELMTLGGELRLAALQPQVHEVFTITGFTQVFNIHPSVTE
ECCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHEEEEEECHHEEEECCCCHHH
ARAAFSQRR
HHHHHHCCC
>Mature Secondary Structure
MESITLTADLDALAKTSAFITAAAERCGLDERATWQVQLAVDEAVTNVIQHAYDPDQPGD
CCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCC
FTLSWQCHDQRFIVTVRDHGRQFDPSTVPVPDITSPLEERQVGGLGIYLITRLMDEVHFE
EEEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCEE
FSPQGGNLLTMVKYLPSDQAEEANEVVVIPINDRIDALTAPRLSKTVNEQISQGARQIVL
ECCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHEEEE
DFSNVPFLSSSGLRALLLIRKELMTLGGELRLAALQPQVHEVFTITGFTQVFNIHPSVTE
ECCCCCEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHEEEEEECHHEEEECCCCHHH
ARAAFSQRR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]