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The map label for this gene is clpB2 [H]

Identifier: 222524408

GI number: 222524408

Start: 1431714

End: 1434194

Strand: Direct

Name: clpB2 [H]

Synonym: Chy400_1132

Alternate gene names: 222524408

Gene position: 1431714-1434194 (Clockwise)

Preceding gene: 222524406

Following gene: 222524409

Centisome position: 27.17

GC content: 56.59

Gene sequence:

>2481_bases
ATGAGACTGGAGCGATTTACCGAAAAAGCGCAAAATGCCTTCCATATTGCGCAAGAGATTATGCAGGAGCATCAACATTC
ACAGCTCGATGTTGAGCATCTCTTTCTGGCGATGCTCCGGCAGCGTGATGGTCTGGCAGCTCGTGCGTTGGCGCGGTTGA
ACGTAGACCCCCACGAGGTGGAACGACGTGTCGAACGCGAGCTGGAAAAGCTCCCTAAGATTCTCAGCCCATACGGTATG
GGCAGTCAGGTCTATATTACGCCACGCACGCAGCGATTAGTCAAGCGGGCAGAGGAAGAGGCTAACCGCCTGGGAGACCA
GTATGTTGGCCTTGATCATCTGCTGATCGGACTGGCCAATGAGCGTGATGGCCCATCGGCGCGGATTTTGCAGAGTTATC
AGATTGATCAGGAGCGGATTTATCAGGTGTTGATGGAGATTCGTGGTAGCCAACGCAGCGATGACCCGTCGGCTGAGAGC
CGCTACGAGATTTTGAGCAAGTATAGCGTCGATTTAACCGAGCTGGCCCGTGAAGATAAGCTCGATCCGGTGATTGGCCG
TGAAGCCGAAATTACGCGGGTCATTCGCATCTTGTCGCGACGGACAAAGAATAATCCGGTGCTGGTCGGCGAAACCGGCG
TCGGCAAGACGGCGATTGTCGAGGGATTGGCCCAGCGTATTGCGCGGGGTGATGTACCACCGACCTTACGTGATCGGAAG
CTGCTGGCGCTCGATCTGGCCGGGATGGTTGCCGGTTCTAAGTTCCGTGGTGAGTTTGAAGAGCGCCTCAAAGCGGTCAT
GGACGAGGTGCGCAATGCGAAGGGGCGGATTATTCTCTTTATCGACGAATTGCATACGGTCGTCGGTGCCGGTGCTGCAA
CCGGTAGCATCGACGCCTCGAATATGCTGAAACCGGCCCTCTCGCGTGGTGAGATTCAGGTGATCGGCGCAACCACCATC
GATGAGTACCGCAAGCATGTCGAGAAAGATGCTGCCCTTGAACGGCGCTTCTCACCGGTTTTCGTCGAAGAACCTGACCC
GGAGACGACGGTCGAGATGCTGCGCGGCCTGCGCAAGCGCTACGAGGAGCATCATCAGCTTACTATCAGCGATGAAGCTC
TACGGGCAGCCGTTCAGCTTTCGAGCCGGTATATTAATGATCGCTTCCTGCCTGACAAGGCGATAGATTTGATCGACGAA
GCCAGTGCCAAGTTGCGCATCGATATCTTCTCGATGCCGCCTGAATTGAAGGCTAAAGAGGAAGAATTGACGCGACTTCA
GGCCGAAGAGGAAGAGGCCGGTGCCCGACGTGATTACGAGCGGGCTGCCCTGCTCCGCTCCCGCTACCTTGCCCTCCAGG
CCCAGTTTGAAGCCGAGCGCAATGAGTGGCTGGCACGCAACAACCTCGATGAAGTTGTCGATGATGAAGATGTGGCCCAG
ATCGTCTCAAGCTGGACGGGTGTGCCGGTCAGTCGTATGCTCGAAACCGAACGCGAGAAGCTCATCCATATGGAAGAGCG
ATTGCACGAGCGCGTGATCGGTCAGCACGAGGCGATTGTCGCCCTGAGCGATGCAATTCGCCGTGCCCGTTCTGGTCTGC
GCGATCCACGGCGCCCGATTGGTTCGTTCCTCTTTGTTGGCCCAACCGGTGTTGGCAAAACTGAGCTGGCGCGTGCCCTC
GCCGAGTTTATGTTTGACAGCGAAGAGGCGATGACCCGCGTCGATATGAGTGAGTATCAGGAACGCCACACCGTCAGTCG
CCTGATCGGTGCGCCACCCGGTTACGTTGGCTATGATGAAGGTGGTCAGTTGACCGAGAGCATTCGCCGCCGACCGTACC
AGGTCGTGCTCTTCGATGAGATCGAAAAGGCGCATCGCGATGTGTTCAATGCCCTCTTGCAGGTGCTTGACGATGGACGC
TTGACCGACGGTCAAGGACGCACGGTCGATTTTCGCAATACCATCATCATCATGACCAGCAATGCCGGCACCGAACATCT
GCAAGGCGGCGGGATTGGTTTCAATGTCGGGGGCAAAAACGCCAAAACGATTGATATGCGTGAGGCGCGAAAGAAGGTCG
ATGAAGCGCTGCGGCAGACCTTCCGGCCCGAATTTTTGAACCGGATCGATGAGATCATTCTCTTCCATCCGCTTTCGATG
GACGATCTGACCAGGATCGTCGAACTCCAGATCGCCGATCTGGTGGAACGGCTGCGCGAACAGCAAATTGGTCTCAGCTT
GACCCAGGCTGCCAAGGAATACCTGGTGCAAGAGGGGTACAATCCGGTCTTTGGTGCACGACCGTTGCGCCGCACCATCC
AGCGCCTCCTCGAAACCCCGATCTCGCGGGAGCTGCTGCGCGGTATGTTCCGGGCCGGTGATCAGATAGAAGTAGATGTC
GAGAATGGTCAACTGGTCTTCCATCGCGGTGGCATCTTGACAATGGAAGAACATCGCCCGGCTGAACCTCTTGATGCCTG
A

Upstream 100 bases:

>100_bases
TCTACCCCTGTTTTCGACCACTTCCTGTGCTATACTACCAGAGCAGATCAATGTTGTGATCTGCTAACCCGCCTCCGGCG
GACACAATAGAGGGACTGCG

Downstream 100 bases:

>100_bases
ACTGTAATCTACGACGCCGGGTTTGTCATCTACATTCACCCGGCGTCGTGGTATTATCGTGGATGAAGATCATCAAGTCC
TTTGTTCCAGATCAGGAGTG

Product: ATPase AAA-2 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 826; Mature: 826

Protein sequence:

>826_residues
MRLERFTEKAQNAFHIAQEIMQEHQHSQLDVEHLFLAMLRQRDGLAARALARLNVDPHEVERRVERELEKLPKILSPYGM
GSQVYITPRTQRLVKRAEEEANRLGDQYVGLDHLLIGLANERDGPSARILQSYQIDQERIYQVLMEIRGSQRSDDPSAES
RYEILSKYSVDLTELAREDKLDPVIGREAEITRVIRILSRRTKNNPVLVGETGVGKTAIVEGLAQRIARGDVPPTLRDRK
LLALDLAGMVAGSKFRGEFEERLKAVMDEVRNAKGRIILFIDELHTVVGAGAATGSIDASNMLKPALSRGEIQVIGATTI
DEYRKHVEKDAALERRFSPVFVEEPDPETTVEMLRGLRKRYEEHHQLTISDEALRAAVQLSSRYINDRFLPDKAIDLIDE
ASAKLRIDIFSMPPELKAKEEELTRLQAEEEEAGARRDYERAALLRSRYLALQAQFEAERNEWLARNNLDEVVDDEDVAQ
IVSSWTGVPVSRMLETEREKLIHMEERLHERVIGQHEAIVALSDAIRRARSGLRDPRRPIGSFLFVGPTGVGKTELARAL
AEFMFDSEEAMTRVDMSEYQERHTVSRLIGAPPGYVGYDEGGQLTESIRRRPYQVVLFDEIEKAHRDVFNALLQVLDDGR
LTDGQGRTVDFRNTIIIMTSNAGTEHLQGGGIGFNVGGKNAKTIDMREARKKVDEALRQTFRPEFLNRIDEIILFHPLSM
DDLTRIVELQIADLVERLREQQIGLSLTQAAKEYLVQEGYNPVFGARPLRRTIQRLLETPISRELLRGMFRAGDQIEVDV
ENGQLVFHRGGILTMEEHRPAEPLDA

Sequences:

>Translated_826_residues
MRLERFTEKAQNAFHIAQEIMQEHQHSQLDVEHLFLAMLRQRDGLAARALARLNVDPHEVERRVERELEKLPKILSPYGM
GSQVYITPRTQRLVKRAEEEANRLGDQYVGLDHLLIGLANERDGPSARILQSYQIDQERIYQVLMEIRGSQRSDDPSAES
RYEILSKYSVDLTELAREDKLDPVIGREAEITRVIRILSRRTKNNPVLVGETGVGKTAIVEGLAQRIARGDVPPTLRDRK
LLALDLAGMVAGSKFRGEFEERLKAVMDEVRNAKGRIILFIDELHTVVGAGAATGSIDASNMLKPALSRGEIQVIGATTI
DEYRKHVEKDAALERRFSPVFVEEPDPETTVEMLRGLRKRYEEHHQLTISDEALRAAVQLSSRYINDRFLPDKAIDLIDE
ASAKLRIDIFSMPPELKAKEEELTRLQAEEEEAGARRDYERAALLRSRYLALQAQFEAERNEWLARNNLDEVVDDEDVAQ
IVSSWTGVPVSRMLETEREKLIHMEERLHERVIGQHEAIVALSDAIRRARSGLRDPRRPIGSFLFVGPTGVGKTELARAL
AEFMFDSEEAMTRVDMSEYQERHTVSRLIGAPPGYVGYDEGGQLTESIRRRPYQVVLFDEIEKAHRDVFNALLQVLDDGR
LTDGQGRTVDFRNTIIIMTSNAGTEHLQGGGIGFNVGGKNAKTIDMREARKKVDEALRQTFRPEFLNRIDEIILFHPLSM
DDLTRIVELQIADLVERLREQQIGLSLTQAAKEYLVQEGYNPVFGARPLRRTIQRLLETPISRELLRGMFRAGDQIEVDV
ENGQLVFHRGGILTMEEHRPAEPLDA
>Mature_826_residues
MRLERFTEKAQNAFHIAQEIMQEHQHSQLDVEHLFLAMLRQRDGLAARALARLNVDPHEVERRVERELEKLPKILSPYGM
GSQVYITPRTQRLVKRAEEEANRLGDQYVGLDHLLIGLANERDGPSARILQSYQIDQERIYQVLMEIRGSQRSDDPSAES
RYEILSKYSVDLTELAREDKLDPVIGREAEITRVIRILSRRTKNNPVLVGETGVGKTAIVEGLAQRIARGDVPPTLRDRK
LLALDLAGMVAGSKFRGEFEERLKAVMDEVRNAKGRIILFIDELHTVVGAGAATGSIDASNMLKPALSRGEIQVIGATTI
DEYRKHVEKDAALERRFSPVFVEEPDPETTVEMLRGLRKRYEEHHQLTISDEALRAAVQLSSRYINDRFLPDKAIDLIDE
ASAKLRIDIFSMPPELKAKEEELTRLQAEEEEAGARRDYERAALLRSRYLALQAQFEAERNEWLARNNLDEVVDDEDVAQ
IVSSWTGVPVSRMLETEREKLIHMEERLHERVIGQHEAIVALSDAIRRARSGLRDPRRPIGSFLFVGPTGVGKTELARAL
AEFMFDSEEAMTRVDMSEYQERHTVSRLIGAPPGYVGYDEGGQLTESIRRRPYQVVLFDEIEKAHRDVFNALLQVLDDGR
LTDGQGRTVDFRNTIIIMTSNAGTEHLQGGGIGFNVGGKNAKTIDMREARKKVDEALRQTFRPEFLNRIDEIILFHPLSM
DDLTRIVELQIADLVERLREQQIGLSLTQAAKEYLVQEGYNPVFGARPLRRTIQRLLETPISRELLRGMFRAGDQIEVDV
ENGQLVFHRGGILTMEEHRPAEPLDA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=319, Percent_Identity=36.9905956112853, Blast_Score=212, Evalue=1e-54,
Organism=Escherichia coli, GI1788943, Length=872, Percent_Identity=46.7889908256881, Blast_Score=754, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=813, Percent_Identity=38.6223862238622, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6320464, Length=716, Percent_Identity=47.0670391061453, Blast_Score=626, Evalue=1e-180,
Organism=Saccharomyces cerevisiae, GI6323002, Length=886, Percent_Identity=39.8419864559819, Blast_Score=591, Evalue=1e-169,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 93918; Mature: 93918

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50151 UVR ; PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLERFTEKAQNAFHIAQEIMQEHQHSQLDVEHLFLAMLRQRDGLAARALARLNVDPHEV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
ERRVERELEKLPKILSPYGMGSQVYITPRTQRLVKRAEEEANRLGDQYVGLDHLLIGLAN
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHCC
ERDGPSARILQSYQIDQERIYQVLMEIRGSQRSDDPSAESRYEILSKYSVDLTELAREDK
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCC
LDPVIGREAEITRVIRILSRRTKNNPVLVGETGVGKTAIVEGLAQRIARGDVPPTLRDRK
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCE
LLALDLAGMVAGSKFRGEFEERLKAVMDEVRNAKGRIILFIDELHTVVGAGAATGSIDAS
EHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHH
NMLKPALSRGEIQVIGATTIDEYRKHVEKDAALERRFSPVFVEEPDPETTVEMLRGLRKR
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHH
YEEHHQLTISDEALRAAVQLSSRYINDRFLPDKAIDLIDEASAKLRIDIFSMPPELKAKE
HHHHCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCH
EELTRLQAEEEEAGARRDYERAALLRSRYLALQAQFEAERNEWLARNNLDEVVDDEDVAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
IVSSWTGVPVSRMLETEREKLIHMEERLHERVIGQHEAIVALSDAIRRARSGLRDPRRPI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
GSFLFVGPTGVGKTELARALAEFMFDSEEAMTRVDMSEYQERHTVSRLIGAPPGYVGYDE
CCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GGQLTESIRRRPYQVVLFDEIEKAHRDVFNALLQVLDDGRLTDGQGRTVDFRNTIIIMTS
CCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEC
NAGTEHLQGGGIGFNVGGKNAKTIDMREARKKVDEALRQTFRPEFLNRIDEIILFHPLSM
CCCCCCCCCCCEEECCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
DDLTRIVELQIADLVERLREQQIGLSLTQAAKEYLVQEGYNPVFGARPLRRTIQRLLETP
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
ISRELLRGMFRAGDQIEVDVENGQLVFHRGGILTMEEHRPAEPLDA
HHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MRLERFTEKAQNAFHIAQEIMQEHQHSQLDVEHLFLAMLRQRDGLAARALARLNVDPHEV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHH
ERRVERELEKLPKILSPYGMGSQVYITPRTQRLVKRAEEEANRLGDQYVGLDHLLIGLAN
HHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHCC
ERDGPSARILQSYQIDQERIYQVLMEIRGSQRSDDPSAESRYEILSKYSVDLTELAREDK
CCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCC
LDPVIGREAEITRVIRILSRRTKNNPVLVGETGVGKTAIVEGLAQRIARGDVPPTLRDRK
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCE
LLALDLAGMVAGSKFRGEFEERLKAVMDEVRNAKGRIILFIDELHTVVGAGAATGSIDAS
EHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCCCCCCHH
NMLKPALSRGEIQVIGATTIDEYRKHVEKDAALERRFSPVFVEEPDPETTVEMLRGLRKR
HHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHH
YEEHHQLTISDEALRAAVQLSSRYINDRFLPDKAIDLIDEASAKLRIDIFSMPPELKAKE
HHHHCCEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCH
EELTRLQAEEEEAGARRDYERAALLRSRYLALQAQFEAERNEWLARNNLDEVVDDEDVAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHH
IVSSWTGVPVSRMLETEREKLIHMEERLHERVIGQHEAIVALSDAIRRARSGLRDPRRPI
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHH
GSFLFVGPTGVGKTELARALAEFMFDSEEAMTRVDMSEYQERHTVSRLIGAPPGYVGYDE
CCEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GGQLTESIRRRPYQVVLFDEIEKAHRDVFNALLQVLDDGRLTDGQGRTVDFRNTIIIMTS
CCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEC
NAGTEHLQGGGIGFNVGGKNAKTIDMREARKKVDEALRQTFRPEFLNRIDEIILFHPLSM
CCCCCCCCCCCEEECCCCCCCCEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCH
DDLTRIVELQIADLVERLREQQIGLSLTQAAKEYLVQEGYNPVFGARPLRRTIQRLLETP
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
ISRELLRGMFRAGDQIEVDVENGQLVFHRGGILTMEEHRPAEPLDA
HHHHHHHHHHCCCCEEEEEECCCEEEEECCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]