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The map label for this gene is cotA [H]

Identifier: 222524347

GI number: 222524347

Start: 1360771

End: 1362411

Strand: Reverse

Name: cotA [H]

Synonym: Chy400_1070

Alternate gene names: 222524347

Gene position: 1362411-1360771 (Counterclockwise)

Preceding gene: 222524348

Following gene: 222524346

Centisome position: 25.86

GC content: 56.98

Gene sequence:

>1641_bases
ATGCTTTCACGACTCACACGGCGGGGTTTGTTGCGCTTGTTTGGCGCAGGCGCTCTCGCCTTTGGCAGTGGCGTTCTGAA
TGGGTGTGCCCTACCGACGCGCTCTTCTTCCATGCCGCAGCCGCAGACCAATCCCAGTCCTGCCTTTACCCCCGATCTCG
ATCTGACCATACGCGCAGTTGCCGCAGAGGTGCCAATCCTGCCGGGAACGCCGACCAGGGTGTGGACATATCAGGCTCAG
GTGAATGCCGGCGATCCGGCAGCAGTACAACCCATTCCCGACAGCTATCTTGGTCCGATTATTCGTGTTCGCACCGGGCA
GAAGGTACGTATCACGTTTGAGAATAAGTTGCCTGATCCCCGGCAGGATTCGATTATTCACTGGCACGGTCTGCATACCC
CACCCGCGATGGATGGGCATCCGCATACAGTGGTACGTCCCGGCGAACGTTATGTCTACGAGTTTACCGTCACCGACCGG
GCCGGTACATACTGGTTTCATCCCCATCCTCACGGCCAGACTGCCCAACAGGTCATTATGGGTCTTGCTGGACTGTTTGT
GGTGAGTGATCCTGCGGAGGATGCTATCGGGTTGCCGGGCGGTGAATATGACCTGCCACTTGTCATTCAAGACCGTACCT
TTGATGCTGACAATCAGTTCGTCTACGACTCAGACCCAATGACCCGCCTGATGGGGTTTCTCGGCGAGCGAATTCTGGTC
AATGGCCGGCCAGACCTGACCATCACCGCCGCGACCCGCGCATATCGCTGGCGCATCCTCAACGGATCGAACAGTCGTAT
CTACAAGCTGGCCTGGGATTCGGGCATGCCACTGACGGTCATTGCCACCGATGGCGGCCTACTTGAACATCCGCTTACCC
GCCCGTTCGTTATGCTGGCGCCGGGTGAGCGGATCGAACTCTGGGTTGATCTCCGCGATATTCCACGCGACACCACCCTC
AAACTGGTAAGTCAGCCGTTCAGTGGCGCAGAGGGCGTTGGCATGATGGGTGAGAGCGGGAATATGGATCACGGGATGGG
TGGTATGCACGGTCAGATGGGAATGATGCGCAGCGGTAATGCCCCGCCCCTTGGTGCACCGCTCGACATTCTCCAGGTGC
GGGTGACACGTCAGGAGCAAGAAACGCTCGTGCTTCCCGCCTCGCTGATACCGATCCAGCGTCATCGGCGGGAAGCAGCT
GTTAATGCGACAACACCACGGCGTATCGCACTCTCGCTGCGTGGTATGGAATGGCAGATCAATGGGAAAACATTCGTGAT
GGACGAAGTGGCGCCGGATGAAATTGTACGGCTGAATACCCTCGAACTCTGGGAGATTGTCAACGAGACGAATCCAGGTG
AAATGATGGACCCCATGGGAATGGCGCATCCCATTCACATTCACGGTGGTCAGTTTCAGATCGTTCAGCGCACGGTGCTT
CCCGAATTACGGGCCGGTTGGGAAGAGGTGAAGGATGGATACGTTGACGAAGGCTGGAAGGATACCGTCCTGGTGATGCC
CGGTGAACGGGTGCAGTTATTGATGGCCTTCCGTGACTATACCGGTATGTATGTCTATCACTGCCATAACCTTGAACACG
AAGATGCCGGCATGATGCGCAATTACCGGATTGAGGCATAA

Upstream 100 bases:

>100_bases
AAGCACCTGGCCGCCCGAAATTCGGGAAGCGCAGGAATCTATCAACCGCCAATCGCCGTAGCTGATTTCTTTCACACCAC
ACTTGCAGAAGGAGAGATCT

Downstream 100 bases:

>100_bases
CGGTCTGACTATATCCTGGCAAACAAAGGATTGGATACATGCAAACATCACCCTCATCACACGACGACCACCAACATGCA
GCAGGACCTGCTCAATTAGC

Product: Bilirubin oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MLSRLTRRGLLRLFGAGALAFGSGVLNGCALPTRSSSMPQPQTNPSPAFTPDLDLTIRAVAAEVPILPGTPTRVWTYQAQ
VNAGDPAAVQPIPDSYLGPIIRVRTGQKVRITFENKLPDPRQDSIIHWHGLHTPPAMDGHPHTVVRPGERYVYEFTVTDR
AGTYWFHPHPHGQTAQQVIMGLAGLFVVSDPAEDAIGLPGGEYDLPLVIQDRTFDADNQFVYDSDPMTRLMGFLGERILV
NGRPDLTITAATRAYRWRILNGSNSRIYKLAWDSGMPLTVIATDGGLLEHPLTRPFVMLAPGERIELWVDLRDIPRDTTL
KLVSQPFSGAEGVGMMGESGNMDHGMGGMHGQMGMMRSGNAPPLGAPLDILQVRVTRQEQETLVLPASLIPIQRHRREAA
VNATTPRRIALSLRGMEWQINGKTFVMDEVAPDEIVRLNTLELWEIVNETNPGEMMDPMGMAHPIHIHGGQFQIVQRTVL
PELRAGWEEVKDGYVDEGWKDTVLVMPGERVQLLMAFRDYTGMYVYHCHNLEHEDAGMMRNYRIEA

Sequences:

>Translated_546_residues
MLSRLTRRGLLRLFGAGALAFGSGVLNGCALPTRSSSMPQPQTNPSPAFTPDLDLTIRAVAAEVPILPGTPTRVWTYQAQ
VNAGDPAAVQPIPDSYLGPIIRVRTGQKVRITFENKLPDPRQDSIIHWHGLHTPPAMDGHPHTVVRPGERYVYEFTVTDR
AGTYWFHPHPHGQTAQQVIMGLAGLFVVSDPAEDAIGLPGGEYDLPLVIQDRTFDADNQFVYDSDPMTRLMGFLGERILV
NGRPDLTITAATRAYRWRILNGSNSRIYKLAWDSGMPLTVIATDGGLLEHPLTRPFVMLAPGERIELWVDLRDIPRDTTL
KLVSQPFSGAEGVGMMGESGNMDHGMGGMHGQMGMMRSGNAPPLGAPLDILQVRVTRQEQETLVLPASLIPIQRHRREAA
VNATTPRRIALSLRGMEWQINGKTFVMDEVAPDEIVRLNTLELWEIVNETNPGEMMDPMGMAHPIHIHGGQFQIVQRTVL
PELRAGWEEVKDGYVDEGWKDTVLVMPGERVQLLMAFRDYTGMYVYHCHNLEHEDAGMMRNYRIEA
>Mature_546_residues
MLSRLTRRGLLRLFGAGALAFGSGVLNGCALPTRSSSMPQPQTNPSPAFTPDLDLTIRAVAAEVPILPGTPTRVWTYQAQ
VNAGDPAAVQPIPDSYLGPIIRVRTGQKVRITFENKLPDPRQDSIIHWHGLHTPPAMDGHPHTVVRPGERYVYEFTVTDR
AGTYWFHPHPHGQTAQQVIMGLAGLFVVSDPAEDAIGLPGGEYDLPLVIQDRTFDADNQFVYDSDPMTRLMGFLGERILV
NGRPDLTITAATRAYRWRILNGSNSRIYKLAWDSGMPLTVIATDGGLLEHPLTRPFVMLAPGERIELWVDLRDIPRDTTL
KLVSQPFSGAEGVGMMGESGNMDHGMGGMHGQMGMMRSGNAPPLGAPLDILQVRVTRQEQETLVLPASLIPIQRHRREAA
VNATTPRRIALSLRGMEWQINGKTFVMDEVAPDEIVRLNTLELWEIVNETNPGEMMDPMGMAHPIHIHGGQFQIVQRTVL
PELRAGWEEVKDGYVDEGWKDTVLVMPGERVQLLMAFRDYTGMYVYHCHNLEHEDAGMMRNYRIEA

Specific function: Involved in brown pigmentation during sporogenesis [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To S.antibioticus phenoxazinone synthase (phsA) [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=236, Percent_Identity=37.7118644067797, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789394, Length=550, Percent_Identity=26.5454545454545, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR008972 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 60716; Mature: 60716

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00080 MULTICOPPER_OXIDASE2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRLTRRGLLRLFGAGALAFGSGVLNGCALPTRSSSMPQPQTNPSPAFTPDLDLTIRAV
CCHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
AAEVPILPGTPTRVWTYQAQVNAGDPAAVQPIPDSYLGPIIRVRTGQKVRITFENKLPDP
EECCCCCCCCCCEEEEEEEEECCCCCCEECCCCCHHHCCEEEEECCCEEEEEECCCCCCC
RQDSIIHWHGLHTPPAMDGHPHTVVRPGERYVYEFTVTDRAGTYWFHPHPHGQTAQQVIM
CCCCEEEEECCCCCCCCCCCCCEEECCCCEEEEEEEEECCCCEEEECCCCCCHHHHHHHH
GLAGLFVVSDPAEDAIGLPGGEYDLPLVIQDRTFDADNQFVYDSDPMTRLMGFLGERILV
HHHCEEEEECCCHHCCCCCCCCCCCEEEEECCCCCCCCCEEECCCHHHHHHHHCCCEEEE
NGRPDLTITAATRAYRWRILNGSNSRIYKLAWDSGMPLTVIATDGGLLEHPLTRPFVMLA
CCCCCEEEEEECEEEEEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEC
PGERIELWVDLRDIPRDTTLKLVSQPFSGAEGVGMMGESGNMDHGMGGMHGQMGMMRSGN
CCCEEEEEEEHHHCCCCHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEECCC
APPLGAPLDILQVRVTRQEQETLVLPASLIPIQRHRREAAVNATTPRRIALSLRGMEWQI
CCCCCCCCEEEEEEEECCCCCEEEECHHHCCHHHHHHHHHCCCCCCCEEEEEEECEEEEE
NGKTFVMDEVAPDEIVRLNTLELWEIVNETNPGEMMDPMGMAHPIHIHGGQFQIVQRTVL
CCEEEEEECCCCCCEEEECHHHHHHHHCCCCCCHHCCCCCCCCEEEEECCEEEEEHHHHH
PELRAGWEEVKDGYVDEGWKDTVLVMPGERVQLLMAFRDYTGMYVYHCHNLEHEDAGMMR
HHHHCCHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCC
NYRIEA
EEEECC
>Mature Secondary Structure
MLSRLTRRGLLRLFGAGALAFGSGVLNGCALPTRSSSMPQPQTNPSPAFTPDLDLTIRAV
CCHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
AAEVPILPGTPTRVWTYQAQVNAGDPAAVQPIPDSYLGPIIRVRTGQKVRITFENKLPDP
EECCCCCCCCCCEEEEEEEEECCCCCCEECCCCCHHHCCEEEEECCCEEEEEECCCCCCC
RQDSIIHWHGLHTPPAMDGHPHTVVRPGERYVYEFTVTDRAGTYWFHPHPHGQTAQQVIM
CCCCEEEEECCCCCCCCCCCCCEEECCCCEEEEEEEEECCCCEEEECCCCCCHHHHHHHH
GLAGLFVVSDPAEDAIGLPGGEYDLPLVIQDRTFDADNQFVYDSDPMTRLMGFLGERILV
HHHCEEEEECCCHHCCCCCCCCCCCEEEEECCCCCCCCCEEECCCHHHHHHHHCCCEEEE
NGRPDLTITAATRAYRWRILNGSNSRIYKLAWDSGMPLTVIATDGGLLEHPLTRPFVMLA
CCCCCEEEEEECEEEEEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEC
PGERIELWVDLRDIPRDTTLKLVSQPFSGAEGVGMMGESGNMDHGMGGMHGQMGMMRSGN
CCCEEEEEEEHHHCCCCHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCEEECCC
APPLGAPLDILQVRVTRQEQETLVLPASLIPIQRHRREAAVNATTPRRIALSLRGMEWQI
CCCCCCCCEEEEEEEECCCCCEEEECHHHCCHHHHHHHHHCCCCCCCEEEEEEECEEEEE
NGKTFVMDEVAPDEIVRLNTLELWEIVNETNPGEMMDPMGMAHPIHIHGGQFQIVQRTVL
CCEEEEEECCCCCCEEEECHHHHHHHHCCCCCCHHCCCCCCCCEEEEECCEEEEEHHHHH
PELRAGWEEVKDGYVDEGWKDTVLVMPGERVQLLMAFRDYTGMYVYHCHNLEHEDAGMMR
HHHHCCHHHHHCCCCCCCCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCC
NYRIEA
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969499; 9455482; 9384377; 2821284; 3135411 [H]