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The map label for this gene is braE [H]

Identifier: 222524294

GI number: 222524294

Start: 1295687

End: 1297036

Strand: Direct

Name: braE [H]

Synonym: Chy400_1017

Alternate gene names: 222524294

Gene position: 1295687-1297036 (Clockwise)

Preceding gene: 222524293

Following gene: 222524295

Centisome position: 24.59

GC content: 55.04

Gene sequence:

>1350_bases
ATGACTACGAGCACTTCGCCGCAGAGCCTTTGGCAGCGCGTAACGACTTCTCTGAGCAGTGGGCCAACCGCATACGCATT
TCTGGTTTTCTACGTGCTCGTCTGCTCCTTCCTGCTCTTGACCAATCCGCGCACCGAATTGAGCACCGACATCGTGTTCG
TGCTCTTTATGTTGTCGATGCTGGTAGCGTATCGCTCGCGGGTTGCCGGTTGGTTCAAGGTGGTACTGGGGGTGTTTGCC
CTTGGTATCGTTTTACCGTACTTCGGTACCCTCAACCCGTTTTACATTGATGTCGCAACTCAGGCTGGTATCTTCACGGC
ACTGGCCCTGGGATTGAACGTGGTCGTCGGCTTTGCCGGCCTCCTTGACCTTGGCTATGTCGCGTTCTTTGCGGTGGGTG
CGTATGCCTGGGCGATCTTTAGCACCCCACAGATCAGCGAGATTAATCCGGCCTGGAGTCCGGTCGATCCGATCATGTTC
TATCCGTTTCTGATTATTGGCCTCATTCTCGGTTTGATCACCGGTGTCTTGCTTGGTTTACCGGTGTTGCGTCTCCGTGG
TGATTATCTGGCTATTGTAACCCTGGGTTTTGGCGAGGTGATTCGAGTTATCGCCAACAACCTGGATCGACCGGTAAATG
TGACCAATGGCTCACAAGGCATTCGCGACATCGAGCGGCCCCCGCTCGTGCTGGTTGATCTGGTGCGAAACCTTGGTGTT
GTTGCCGACGATAATCGCCTGTATCAGCTCTTCTTCTACTACCTGGTTCTGATCATTCTGTTGCTCACCGTGATCGTCGT
AGCTCGCTTGAAGAATTCACGGATTGGTCGGGCCTGGGAAGCGATTCGTGAAGACGAGGTGGCAGCAGTTGCGCAGGGTA
TCCCACGGGTGCGCATGAAACTGCTGGCTTTCGCGATTGGCGCATCATTTGCCGGTGTCATGGGGGTGGTCTTCGCCTCG
AAGCAGCTCTTTATCAATCCACCAACCTTTGACTTGCTGCGCTCCATCAATATCCTGGTGATGGTTATCCTCGGTGGGAT
GGGTTCCATTCCTGGGGCAATGCTCGGTGCAATCATTGTAACGATGCTCGATTTGCAGATATTGCCCCGCACGGCTGCTG
TGATCCGCGAAGCCCAGGCTGCCGGTTTACCCATCCCGCGCCAGTTCGATCCTACTCAGTATCAGCGACTCTTCTTTGGG
CTGTTGCTGGTGATTATGATGATCTTCCGTCCTGAAGGATTGTGGCCTGACGAGCGCCGGCGCGAAGAGCAGCACGAGGT
CGAAGTGACTGAAGAGGAGCTTGAGGCCGACAAGTCGGTGGCACCAACCGGTGCCGATATGGGGAGATAG

Upstream 100 bases:

>100_bases
CCTATGCCGATATTGCCGCGTTTAGTATCCTGATCTTGCTGTTAATTTTCCGGCCTTCCGGTCTGCTCGGTGAGGCGACA
ACGCAGAAGGTGTAAGCAGT

Downstream 100 bases:

>100_bases
CCATGGCGCTTTTAGAGGCTTCTAATATTACAAAAGTCTTCGGTGGTCTGGTGGCGGTTAACGATGTAACCTTCACCGTT
GAACCGGGCAGCATCGTCAG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTTSTSPQSLWQRVTTSLSSGPTAYAFLVFYVLVCSFLLLTNPRTELSTDIVFVLFMLSMLVAYRSRVAGWFKVVLGVFA
LGIVLPYFGTLNPFYIDVATQAGIFTALALGLNVVVGFAGLLDLGYVAFFAVGAYAWAIFSTPQISEINPAWSPVDPIMF
YPFLIIGLILGLITGVLLGLPVLRLRGDYLAIVTLGFGEVIRVIANNLDRPVNVTNGSQGIRDIERPPLVLVDLVRNLGV
VADDNRLYQLFFYYLVLIILLLTVIVVARLKNSRIGRAWEAIREDEVAAVAQGIPRVRMKLLAFAIGASFAGVMGVVFAS
KQLFINPPTFDLLRSINILVMVILGGMGSIPGAMLGAIIVTMLDLQILPRTAAVIREAQAAGLPIPRQFDPTQYQRLFFG
LLLVIMMIFRPEGLWPDERRREEQHEVEVTEEELEADKSVAPTGADMGR

Sequences:

>Translated_449_residues
MTTSTSPQSLWQRVTTSLSSGPTAYAFLVFYVLVCSFLLLTNPRTELSTDIVFVLFMLSMLVAYRSRVAGWFKVVLGVFA
LGIVLPYFGTLNPFYIDVATQAGIFTALALGLNVVVGFAGLLDLGYVAFFAVGAYAWAIFSTPQISEINPAWSPVDPIMF
YPFLIIGLILGLITGVLLGLPVLRLRGDYLAIVTLGFGEVIRVIANNLDRPVNVTNGSQGIRDIERPPLVLVDLVRNLGV
VADDNRLYQLFFYYLVLIILLLTVIVVARLKNSRIGRAWEAIREDEVAAVAQGIPRVRMKLLAFAIGASFAGVMGVVFAS
KQLFINPPTFDLLRSINILVMVILGGMGSIPGAMLGAIIVTMLDLQILPRTAAVIREAQAAGLPIPRQFDPTQYQRLFFG
LLLVIMMIFRPEGLWPDERRREEQHEVEVTEEELEADKSVAPTGADMGR
>Mature_448_residues
TTSTSPQSLWQRVTTSLSSGPTAYAFLVFYVLVCSFLLLTNPRTELSTDIVFVLFMLSMLVAYRSRVAGWFKVVLGVFAL
GIVLPYFGTLNPFYIDVATQAGIFTALALGLNVVVGFAGLLDLGYVAFFAVGAYAWAIFSTPQISEINPAWSPVDPIMFY
PFLIIGLILGLITGVLLGLPVLRLRGDYLAIVTLGFGEVIRVIANNLDRPVNVTNGSQGIRDIERPPLVLVDLVRNLGVV
ADDNRLYQLFFYYLVLIILLLTVIVVARLKNSRIGRAWEAIREDEVAAVAQGIPRVRMKLLAFAIGASFAGVMGVVFASK
QLFINPPTFDLLRSINILVMVILGGMGSIPGAMLGAIIVTMLDLQILPRTAAVIREAQAAGLPIPRQFDPTQYQRLFFGL
LLVIMMIFRPEGLWPDERRREEQHEVEVTEEELEADKSVAPTGADMGR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=349, Percent_Identity=37.8223495702006, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 49348; Mature: 49217

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTSTSPQSLWQRVTTSLSSGPTAYAFLVFYVLVCSFLLLTNPRTELSTDIVFVLFMLSM
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH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HHHHHHHHCCHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]