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The map label for this gene is ysgA [H]

Identifier: 222524019

GI number: 222524019

Start: 919004

End: 919789

Strand: Direct

Name: ysgA [H]

Synonym: Chy400_0734

Alternate gene names: 222524019

Gene position: 919004-919789 (Clockwise)

Preceding gene: 222524018

Following gene: 222524020

Centisome position: 17.44

GC content: 63.49

Gene sequence:

>786_bases
ATGATGATCACCAGCCTCGCCAATCCAACCATCAAAGCCCTGCGTGCGCTCAGGCAACGCAAAGCTCGCGACGAGCAGGG
GCGCTACCTGATTGAAGGGATTCGCCCGGTCGCCGAAGCGATTCAATGCGGTGCACCGCTCGACATGCTGGTCGTTGCCC
CTGAACTGCTCAACAGCAGCTTTGCGCACGAACTGATCGACACCTATGCTGCTGGCGGCGGCAAAATACTGACCGTCAGT
GCAGCAGTCTTCAGCAGCCTGGCCGACAAAGACAACCCGCAGGGCCTGGCAGCCGTCGGGCGCATACACTACACCCCCCT
CCCCAACCTGTCGCCGGCGCCGGTTGGCTGGGTGGTACTGGTTGAAGTAGCCGATCCGGGCAACCTGGGCACAATCTTAC
GCACTGCCGATGGTGCCGGCTTCAGCGGCGTCATCCTGGTCGGCGCAACGACCGACCCCTTCGACCCGGCAGCGGTGCGG
GCCAGCATGGGCGCCCTCTTCAGCCAACAGATTGCACGCACGAGCTGGCCCGATCTGCTGACCTGGGCACGACAGCACCG
TATCGCCCTGGTCGGCAGCTCTGATCGCGGAGCCAGTGACTACCGGGCCACAACCTACCCGCGACCACTGCTCCTGGTGC
TGGGCAGCGAACGGCACGGCCTGACGGACGAACAGCTTGCCGCCTGTGATCTGGTTGTCCGTATTCCAATGCGGGGGCGT
AGCGACTCGCTCAATCTGGCAGTGGCAGCCGGCATCCTGATGTACGAAGCGACCCGCTCACAATAA

Upstream 100 bases:

>100_bases
TGACCCAGGCGCTCGGTGAGGCCCAGCTTGCTGTGCAACGTCAGATCAAAGCCCATTTCGACCCCACCGGTCGGCTGAAT
CCTGGCAAAGTACTCCCGGT

Downstream 100 bases:

>100_bases
TCAGCGGTTGCCCAGCCGACATCGCCCCGGATGGGTGCAGACGGTGTCTTTCCCCACGTGCCTCATATGATGTTGTGATA
GTCGCAATTCACCCGTCTCC

Product: tRNA/rRNA methyltransferase SpoU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MMITSLANPTIKALRALRQRKARDEQGRYLIEGIRPVAEAIQCGAPLDMLVVAPELLNSSFAHELIDTYAAGGGKILTVS
AAVFSSLADKDNPQGLAAVGRIHYTPLPNLSPAPVGWVVLVEVADPGNLGTILRTADGAGFSGVILVGATTDPFDPAAVR
ASMGALFSQQIARTSWPDLLTWARQHRIALVGSSDRGASDYRATTYPRPLLLVLGSERHGLTDEQLAACDLVVRIPMRGR
SDSLNLAVAAGILMYEATRSQ

Sequences:

>Translated_261_residues
MMITSLANPTIKALRALRQRKARDEQGRYLIEGIRPVAEAIQCGAPLDMLVVAPELLNSSFAHELIDTYAAGGGKILTVS
AAVFSSLADKDNPQGLAAVGRIHYTPLPNLSPAPVGWVVLVEVADPGNLGTILRTADGAGFSGVILVGATTDPFDPAAVR
ASMGALFSQQIARTSWPDLLTWARQHRIALVGSSDRGASDYRATTYPRPLLLVLGSERHGLTDEQLAACDLVVRIPMRGR
SDSLNLAVAAGILMYEATRSQ
>Mature_261_residues
MMITSLANPTIKALRALRQRKARDEQGRYLIEGIRPVAEAIQCGAPLDMLVVAPELLNSSFAHELIDTYAAGGGKILTVS
AAVFSSLADKDNPQGLAAVGRIHYTPLPNLSPAPVGWVVLVEVADPGNLGTILRTADGAGFSGVILVGATTDPFDPAAVR
ASMGALFSQQIARTSWPDLLTWARQHRIALVGSSDRGASDYRATTYPRPLLLVLGSERHGLTDEQLAACDLVVRIPMRGR
SDSLNLAVAAGILMYEATRSQ

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=301, Percent_Identity=25.9136212624585, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1790623, Length=145, Percent_Identity=31.7241379310345, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790083, Length=144, Percent_Identity=31.25, Blast_Score=68, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24666840, Length=296, Percent_Identity=25.6756756756757, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27737; Mature: 27737

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMITSLANPTIKALRALRQRKARDEQGRYLIEGIRPVAEAIQCGAPLDMLVVAPELLNSS
CEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEECHHHHHHH
FAHELIDTYAAGGGKILTVSAAVFSSLADKDNPQGLAAVGRIHYTPLPNLSPAPVGWVVL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
VEVADPGNLGTILRTADGAGFSGVILVGATTDPFDPAAVRASMGALFSQQIARTSWPDLL
EEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
TWARQHRIALVGSSDRGASDYRATTYPRPLLLVLGSERHGLTDEQLAACDLVVRIPMRGR
HHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHEEEEEECCCCCC
SDSLNLAVAAGILMYEATRSQ
CCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMITSLANPTIKALRALRQRKARDEQGRYLIEGIRPVAEAIQCGAPLDMLVVAPELLNSS
CEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEECHHHHHHH
FAHELIDTYAAGGGKILTVSAAVFSSLADKDNPQGLAAVGRIHYTPLPNLSPAPVGWVVL
HHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEE
VEVADPGNLGTILRTADGAGFSGVILVGATTDPFDPAAVRASMGALFSQQIARTSWPDLL
EEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
TWARQHRIALVGSSDRGASDYRATTYPRPLLLVLGSERHGLTDEQLAACDLVVRIPMRGR
HHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHEEEEEECCCCCC
SDSLNLAVAAGILMYEATRSQ
CCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]