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The map label for this gene is radA [H]

Identifier: 222523773

GI number: 222523773

Start: 576603

End: 578006

Strand: Direct

Name: radA [H]

Synonym: Chy400_0480

Alternate gene names: 222523773

Gene position: 576603-578006 (Clockwise)

Preceding gene: 222523762

Following gene: 222523774

Centisome position: 10.94

GC content: 57.26

Gene sequence:

>1404_bases
ATGGCGAAAACACGTACTGTCTTCGTTTGTCAACAATGTGGTGCTCAGCAGAGTCGCTGGATGGGGAAGTGTCCTGAATG
CGGCGCGTGGGACAGTCTGGTTGAGCAGATTGTTACGCCTGCCCGTGGCACAGGCGGTCAGCGGGCTGCTACTGTCGGCC
AGAATCAACCGATCCGACTGCGCGATGTTCCCCTGACCGACTTTCAGCGGCTGCCCGTCTACGCCGAGGAGTTTGCCCGT
GTACTCGGTGGTGGTCTGGTACCCGGCTCGGTGGTGTTGATCGGTGGCGACCCTGGAGTAGGAAAATCAACGCTGCTCGG
TCAGGTAGCGGCCCATTTTGCGGCCAGCGTGGGGCCGGCCCTTTACGTGAGTGCCGAAGAGTCGGCGCAACAGATTCGGT
TACGCGCCGAACGATTAGGATTAACCGCCGATGACCTCTTTCTCCTGGCCGAAACATCACTTGATCTGATTATTGAACAT
ATCCGTCATTTACGGCCACGACTGGTGATCGTCGACTCGATCCAGACGGTCTATCTCCCCGAATTAACCAGCGCAGCCGG
TAGTGTCTCGCAGGTACGCGAGGGTGCCCTTCGCCTGATGCAACTGGCGAAAGAGACTGCCATTCCGATTGTGCTGGTTG
GTCACGTAACGAAAGAGGGAGCCATCGCCGGCCCACGTGTCCTCGAACACATTGTTGATGTCGTGCTCTACCTCGAAGGC
GACCGGTTTCACCAGTTTCGCCTGTTACGCGGGGTCAAAAATCGCTTCGGTAGCACGAATGAAGTGGGCGTCTTTGAAAT
GACCGGTGATGGTCTGCGTGAGGTGCGTAATCCGTCGCAGGTCTTTCTCGCCGAACGGAATACGCAAAGCCCTGGTTCGA
CGGTGACCGTGACGCTAGAGGGAACACGTCCGTTACTGGTAGAAGTACAGGCGTTGACCTCCCATACCGGGAATGCCCAA
CCTCGTCGTACCACCAATGGCTTTGATCTCAATCGCCTGCTCATGCTGACAGCCGTGCTCTCCAAACGAGTAGGGCTGCC
GTTATTCAATCAAGACCTTTATGTCAACATTGTCGGTGGTCTGCGTATCGGTGAACCGGCAGCCGATCTGGCAGTTGCAC
TGGCGATTGCGTCCTCATACCGCAACCAGCGGATTGATCCCGATCTGGTCTGCCTTGGTGAAATCGGGTTGTCAGGCGAA
CTGCGTAGTGTCAGTCAGCTTGAGCGTCGCCTGAGCGAAGCAGCAAAGCTGGGATTTCGGCGAGCAATTGTGCCCGCACA
CTCCACGCCTGACCGGGCAGACGGTCTCACCATCACACCGGTACGCTCACTATCCGAAGCGGTCGAACTCCTCACCCGCA
AAGGAATAGCTTCGTCATCCTCGCCAGCCATCGAACAAGGGTGA

Upstream 100 bases:

>100_bases
TTGCTTCAGACTGTTGAGCCTCATCTGACTGTATGATACTTCTCAACAGCAACACCTCGCACCGATTCGTTGCCTGCGTA
TGCGGTGGTATAATGCAGCT

Downstream 100 bases:

>100_bases
GCTATGAGACTAAGTCTCGATCTTATTGTGCGTTTATTCGGTTTTAGTTGGATGGCCTATCTGGGGTTTTGGGTTGGTCA
TTCACTCTCCGGCCCAACAC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAKTRTVFVCQQCGAQQSRWMGKCPECGAWDSLVEQIVTPARGTGGQRAATVGQNQPIRLRDVPLTDFQRLPVYAEEFAR
VLGGGLVPGSVVLIGGDPGVGKSTLLGQVAAHFAASVGPALYVSAEESAQQIRLRAERLGLTADDLFLLAETSLDLIIEH
IRHLRPRLVIVDSIQTVYLPELTSAAGSVSQVREGALRLMQLAKETAIPIVLVGHVTKEGAIAGPRVLEHIVDVVLYLEG
DRFHQFRLLRGVKNRFGSTNEVGVFEMTGDGLREVRNPSQVFLAERNTQSPGSTVTVTLEGTRPLLVEVQALTSHTGNAQ
PRRTTNGFDLNRLLMLTAVLSKRVGLPLFNQDLYVNIVGGLRIGEPAADLAVALAIASSYRNQRIDPDLVCLGEIGLSGE
LRSVSQLERRLSEAAKLGFRRAIVPAHSTPDRADGLTITPVRSLSEAVELLTRKGIASSSSPAIEQG

Sequences:

>Translated_467_residues
MAKTRTVFVCQQCGAQQSRWMGKCPECGAWDSLVEQIVTPARGTGGQRAATVGQNQPIRLRDVPLTDFQRLPVYAEEFAR
VLGGGLVPGSVVLIGGDPGVGKSTLLGQVAAHFAASVGPALYVSAEESAQQIRLRAERLGLTADDLFLLAETSLDLIIEH
IRHLRPRLVIVDSIQTVYLPELTSAAGSVSQVREGALRLMQLAKETAIPIVLVGHVTKEGAIAGPRVLEHIVDVVLYLEG
DRFHQFRLLRGVKNRFGSTNEVGVFEMTGDGLREVRNPSQVFLAERNTQSPGSTVTVTLEGTRPLLVEVQALTSHTGNAQ
PRRTTNGFDLNRLLMLTAVLSKRVGLPLFNQDLYVNIVGGLRIGEPAADLAVALAIASSYRNQRIDPDLVCLGEIGLSGE
LRSVSQLERRLSEAAKLGFRRAIVPAHSTPDRADGLTITPVRSLSEAVELLTRKGIASSSSPAIEQG
>Mature_466_residues
AKTRTVFVCQQCGAQQSRWMGKCPECGAWDSLVEQIVTPARGTGGQRAATVGQNQPIRLRDVPLTDFQRLPVYAEEFARV
LGGGLVPGSVVLIGGDPGVGKSTLLGQVAAHFAASVGPALYVSAEESAQQIRLRAERLGLTADDLFLLAETSLDLIIEHI
RHLRPRLVIVDSIQTVYLPELTSAAGSVSQVREGALRLMQLAKETAIPIVLVGHVTKEGAIAGPRVLEHIVDVVLYLEGD
RFHQFRLLRGVKNRFGSTNEVGVFEMTGDGLREVRNPSQVFLAERNTQSPGSTVTVTLEGTRPLLVEVQALTSHTGNAQP
RRTTNGFDLNRLLMLTAVLSKRVGLPLFNQDLYVNIVGGLRIGEPAADLAVALAIASSYRNQRIDPDLVCLGEIGLSGEL
RSVSQLERRLSEAAKLGFRRAIVPAHSTPDRADGLTITPVRSLSEAVELLTRKGIASSSSPAIEQG

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=47.6086956521739, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 50257; Mature: 50126

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTRTVFVCQQCGAQQSRWMGKCPECGAWDSLVEQIVTPARGTGGQRAATVGQNQPIRL
CCCCEEEEEEHHCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCEEE
RDVPLTDFQRLPVYAEEFARVLGGGLVPGSVVLIGGDPGVGKSTLLGQVAAHFAASVGPA
ECCCCCCHHHCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCE
LYVSAEESAQQIRLRAERLGLTADDLFLLAETSLDLIIEHIRHLRPRLVIVDSIQTVYLP
EEEECHHHHHHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHHHCCCCEEEEECCCEEECC
ELTSAAGSVSQVREGALRLMQLAKETAIPIVLVGHVTKEGAIAGPRVLEHIVDVVLYLEG
CHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
DRFHQFRLLRGVKNRFGSTNEVGVFEMTGDGLREVRNPSQVFLAERNTQSPGSTVTVTLE
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCCCCEEEEECCCCCCCCEEEEEEE
GTRPLLVEVQALTSHTGNAQPRRTTNGFDLNRLLMLTAVLSKRVGLPLFNQDLYVNIVGG
CCCCEEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEECC
LRIGEPAADLAVALAIASSYRNQRIDPDLVCLGEIGLSGELRSVSQLERRLSEAAKLGFR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RAIVPAHSTPDRADGLTITPVRSLSEAVELLTRKGIASSSSPAIEQG
HEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKTRTVFVCQQCGAQQSRWMGKCPECGAWDSLVEQIVTPARGTGGQRAATVGQNQPIRL
CCCEEEEEEHHCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCEEE
RDVPLTDFQRLPVYAEEFARVLGGGLVPGSVVLIGGDPGVGKSTLLGQVAAHFAASVGPA
ECCCCCCHHHCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCE
LYVSAEESAQQIRLRAERLGLTADDLFLLAETSLDLIIEHIRHLRPRLVIVDSIQTVYLP
EEEECHHHHHHHHHHHHHCCCCHHHEEEEEHHHHHHHHHHHHHCCCCEEEEECCCEEECC
ELTSAAGSVSQVREGALRLMQLAKETAIPIVLVGHVTKEGAIAGPRVLEHIVDVVLYLEG
CHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
DRFHQFRLLRGVKNRFGSTNEVGVFEMTGDGLREVRNPSQVFLAERNTQSPGSTVTVTLE
CCHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCCCCEEEEECCCCCCCCEEEEEEE
GTRPLLVEVQALTSHTGNAQPRRTTNGFDLNRLLMLTAVLSKRVGLPLFNQDLYVNIVGG
CCCCEEEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCEEEEEECC
LRIGEPAADLAVALAIASSYRNQRIDPDLVCLGEIGLSGELRSVSQLERRLSEAAKLGFR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHH
RAIVPAHSTPDRADGLTITPVRSLSEAVELLTRKGIASSSSPAIEQG
HEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]