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The map label for this gene is kstD [H]

Identifier: 222523696

GI number: 222523696

Start: 475189

End: 476862

Strand: Reverse

Name: kstD [H]

Synonym: Chy400_0402

Alternate gene names: 222523696

Gene position: 476862-475189 (Counterclockwise)

Preceding gene: 222523697

Following gene: 222523695

Centisome position: 9.05

GC content: 58.0

Gene sequence:

>1674_bases
ATGGATCGATCTGATTACCAGACGGACGTTCTCATTGTCGGGAGCGGAGCCGGTGCGCTGGTGGCAGCACTGGTGGCGAA
GGAGGCCGGACTCGAGACACTGGTGATCGAAAAAACTGATCTGGTAGGTGGTTCAAGCGCTATGTCGGGTGGCGGATTGT
GGATTCCCAACAATCCGGTGTCGCGCGAGGCCGGGGTAGAAGATTCGTTTGAGATGGCGTGGCGTTATTTAGAAGAGGTG
GTACCGGCTGCCGGTCCCGCTGCCTCACCCGATCGGCGGCGAGCTTTTTTAGAGTACGGGCCACAGATGGTTGAGTTTCT
GCGTCGCCTTGGCTTTCGGTGGCATGCCGCCATCGGGTATCCAGATTACTACCCTGAACGTCCTGGTGGCTCGAAACGCG
GGCGCGCCATCGAGGGAAGCATCTTCGACGGCCGTCAATTAGGGCCATGGCTGAAACGGCTCCGTCCTTTCCCGGGTGCG
CCACCAATACCGATACATACAAATGAGGTTGCTTCTTTTTTGGTTTCGTTGCGTACAGTGCGCGGATTCCTCATGGCCAT
GCGTGTCTTTGGGCGGTTCTTTCAACGAGCGCTGGGTAAGGTGCCGATGGGTCTGGGGGCGTCGCTCGTAGGCCAACTGC
TCTGGTTATGTCTGCAACGGAATATCCCGATCTGGCTCGAAAGCTCTCTCGAAGAATTGGTCGTTGATCATGGACGGGTT
GCCGGCGCTGTTGTGCGCCGTGAAGGGCGCACTCTGCGTGTGGCAGCTCGTAGAGGGGTGTTGCTGGCAGCGGGTGGTTT
TGAGCATCACGGCGAGATGCGTGAACGCTATCTTCCCAAGCCCACGAGTACCGACTGGGCTGTAGGTGCATCGGGCAATA
CAGGCGATGCCATTCGTGCCGCCCAGGCTCTCGGTGCGGCGGTAGCCTTAATGGATAAGGCCTGGGGTGGGCCTGTGGTG
ATACTCCCGCAAGGTCAGGTGTCGTTTCTGGTCGGCGAGCGCTCGTTGCCGTTCTCCATCATTGTTGATAGCAAGGGCGA
GCGTTTCATGAACGAGTCGGCTTCATATGTCGATTGCTGGCGTTGGCAATATGAGCGGCACAGGCAGACGCCGGCCATTC
CGGCCTGGCTGATCATGGAAAGCCGTCATCGCCGCTACTATCCGTTTGGGTTATTGCCGCCGGGCATTACGCCGCAATCG
GCCACCAGCTCCCAATTCCTGGTGCGCGCGCAAACATTGCGAGAGCTGGCGATGCGTATTGGTATCGATCCCGATGGGTT
AGAACGCACAGTAGTGCGGTTTAATCGAATGGCACAGCAGGGTAAGGATGAAGATTTCCACCGTGGTGAGAGTGCTTACG
ACCGTTTCTACAGCGATCCGCGCGTAAAGCCCAACCCCAATCTCGGCCCGCTAGAACGTCCACCTTTCTATGCGACGGCT
ATCTATCCCGGCGAACTGGGGACACTGGGTGGTCTGTTGACAGATGAGTGGGGACAGGTGCTGCACGAAAATGGCGAGCC
TATTCCCGGTCTGTACGCCACAGGCAACACCTCGGCCTCAGTGTTCGGAGGCACTTATCCAGGCCCCGGTTCCACGTTAG
GCCCGGCGTGTACCTTTGCCTACATAGCCATGCAGCATATCGTGCAAAATATGCAACTTGTCGAGCGGGTGTAA

Upstream 100 bases:

>100_bases
GACTGGCTCAGCCGACTGAATCCCCGCTGGGCAGCCGAATTGATCTACAGGCAGATGCAACATCTGTTGTCTCGGTAATG
TTCAATACAGGAGAACGCAT

Downstream 100 bases:

>100_bases
TATCACATTGGCGCATCATTACCTATCACCGGATATGCGGTGAACCGTGACTATCCATCTCATCCGCAATGATCCGTAGG
GATCACCTGCATGATAGGGT

Product: 3-oxosteroid 1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MDRSDYQTDVLIVGSGAGALVAALVAKEAGLETLVIEKTDLVGGSSAMSGGGLWIPNNPVSREAGVEDSFEMAWRYLEEV
VPAAGPAASPDRRRAFLEYGPQMVEFLRRLGFRWHAAIGYPDYYPERPGGSKRGRAIEGSIFDGRQLGPWLKRLRPFPGA
PPIPIHTNEVASFLVSLRTVRGFLMAMRVFGRFFQRALGKVPMGLGASLVGQLLWLCLQRNIPIWLESSLEELVVDHGRV
AGAVVRREGRTLRVAARRGVLLAAGGFEHHGEMRERYLPKPTSTDWAVGASGNTGDAIRAAQALGAAVALMDKAWGGPVV
ILPQGQVSFLVGERSLPFSIIVDSKGERFMNESASYVDCWRWQYERHRQTPAIPAWLIMESRHRRYYPFGLLPPGITPQS
ATSSQFLVRAQTLRELAMRIGIDPDGLERTVVRFNRMAQQGKDEDFHRGESAYDRFYSDPRVKPNPNLGPLERPPFYATA
IYPGELGTLGGLLTDEWGQVLHENGEPIPGLYATGNTSASVFGGTYPGPGSTLGPACTFAYIAMQHIVQNMQLVERV

Sequences:

>Translated_557_residues
MDRSDYQTDVLIVGSGAGALVAALVAKEAGLETLVIEKTDLVGGSSAMSGGGLWIPNNPVSREAGVEDSFEMAWRYLEEV
VPAAGPAASPDRRRAFLEYGPQMVEFLRRLGFRWHAAIGYPDYYPERPGGSKRGRAIEGSIFDGRQLGPWLKRLRPFPGA
PPIPIHTNEVASFLVSLRTVRGFLMAMRVFGRFFQRALGKVPMGLGASLVGQLLWLCLQRNIPIWLESSLEELVVDHGRV
AGAVVRREGRTLRVAARRGVLLAAGGFEHHGEMRERYLPKPTSTDWAVGASGNTGDAIRAAQALGAAVALMDKAWGGPVV
ILPQGQVSFLVGERSLPFSIIVDSKGERFMNESASYVDCWRWQYERHRQTPAIPAWLIMESRHRRYYPFGLLPPGITPQS
ATSSQFLVRAQTLRELAMRIGIDPDGLERTVVRFNRMAQQGKDEDFHRGESAYDRFYSDPRVKPNPNLGPLERPPFYATA
IYPGELGTLGGLLTDEWGQVLHENGEPIPGLYATGNTSASVFGGTYPGPGSTLGPACTFAYIAMQHIVQNMQLVERV
>Mature_557_residues
MDRSDYQTDVLIVGSGAGALVAALVAKEAGLETLVIEKTDLVGGSSAMSGGGLWIPNNPVSREAGVEDSFEMAWRYLEEV
VPAAGPAASPDRRRAFLEYGPQMVEFLRRLGFRWHAAIGYPDYYPERPGGSKRGRAIEGSIFDGRQLGPWLKRLRPFPGA
PPIPIHTNEVASFLVSLRTVRGFLMAMRVFGRFFQRALGKVPMGLGASLVGQLLWLCLQRNIPIWLESSLEELVVDHGRV
AGAVVRREGRTLRVAARRGVLLAAGGFEHHGEMRERYLPKPTSTDWAVGASGNTGDAIRAAQALGAAVALMDKAWGGPVV
ILPQGQVSFLVGERSLPFSIIVDSKGERFMNESASYVDCWRWQYERHRQTPAIPAWLIMESRHRRYYPFGLLPPGITPQS
ATSSQFLVRAQTLRELAMRIGIDPDGLERTVVRFNRMAQQGKDEDFHRGESAYDRFYSDPRVKPNPNLGPLERPPFYATA
IYPGELGTLGGLLTDEWGQVLHENGEPIPGLYATGNTSASVFGGTYPGPGSTLGPACTFAYIAMQHIVQNMQLVERV

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 61202; Mature: 61202

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSDYQTDVLIVGSGAGALVAALVAKEAGLETLVIEKTDLVGGSSAMSGGGLWIPNNPV
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEECCCCCC
SREAGVEDSFEMAWRYLEEVVPAAGPAASPDRRRAFLEYGPQMVEFLRRLGFRWHAAIGY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEEECCC
PDYYPERPGGSKRGRAIEGSIFDGRQLGPWLKRLRPFPGAPPIPIHTNEVASFLVSLRTV
CCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
RGFLMAMRVFGRFFQRALGKVPMGLGASLVGQLLWLCLQRNIPIWLESSLEELVVDHGRV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCHH
AGAVVRREGRTLRVAARRGVLLAAGGFEHHGEMRERYLPKPTSTDWAVGASGNTGDAIRA
HHHHHHCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHH
AQALGAAVALMDKAWGGPVVILPQGQVSFLVGERSLPFSIIVDSKGERFMNESASYVDCW
HHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCEEEEECCCCCHHHHCCCCHHHHH
RWQYERHRQTPAIPAWLIMESRHRRYYPFGLLPPGITPQSATSSQFLVRAQTLRELAMRI
HHHHHHHCCCCCCCHHHEECCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GIDPDGLERTVVRFNRMAQQGKDEDFHRGESAYDRFYSDPRVKPNPNLGPLERPPFYATA
CCCCCHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
IYPGELGTLGGLLTDEWGQVLHENGEPIPGLYATGNTSASVFGGTYPGPGSTLGPACTFA
ECCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCCCCCCCCCCCHHHHHH
YIAMQHIVQNMQLVERV
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRSDYQTDVLIVGSGAGALVAALVAKEAGLETLVIEKTDLVGGSSAMSGGGLWIPNNPV
CCCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEECCCCCC
SREAGVEDSFEMAWRYLEEVVPAAGPAASPDRRRAFLEYGPQMVEFLRRLGFRWHAAIGY
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCEEEEECCC
PDYYPERPGGSKRGRAIEGSIFDGRQLGPWLKRLRPFPGAPPIPIHTNEVASFLVSLRTV
CCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
RGFLMAMRVFGRFFQRALGKVPMGLGASLVGQLLWLCLQRNIPIWLESSLEELVVDHGRV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCHH
AGAVVRREGRTLRVAARRGVLLAAGGFEHHGEMRERYLPKPTSTDWAVGASGNTGDAIRA
HHHHHHCCCCEEEEEECCCEEEEECCCHHHHHHHHHCCCCCCCCCEEECCCCCCCHHHHH
AQALGAAVALMDKAWGGPVVILPQGQVSFLVGERSLPFSIIVDSKGERFMNESASYVDCW
HHHHHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCEEEEECCCCCHHHHCCCCHHHHH
RWQYERHRQTPAIPAWLIMESRHRRYYPFGLLPPGITPQSATSSQFLVRAQTLRELAMRI
HHHHHHHCCCCCCCHHHEECCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GIDPDGLERTVVRFNRMAQQGKDEDFHRGESAYDRFYSDPRVKPNPNLGPLERPPFYATA
CCCCCHHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
IYPGELGTLGGLLTDEWGQVLHENGEPIPGLYATGNTSASVFGGTYPGPGSTLGPACTFA
ECCCCHHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCCEECCCCCCCCCCCCCHHHHHH
YIAMQHIVQNMQLVERV
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]