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The map label for this gene is kanE [H]

Identifier: 222523335

GI number: 222523335

Start: 37894

End: 38838

Strand: Reverse

Name: kanE [H]

Synonym: Chy400_0036

Alternate gene names: 222523335

Gene position: 38838-37894 (Counterclockwise)

Preceding gene: 222523336

Following gene: 222523334

Centisome position: 0.74

GC content: 54.71

Gene sequence:

>945_bases
ATGCGAGCAATTTTTTTCAATGGTGACCTGCAATATACCACCGATTATCCTGACCCCGTTCGTCAGCCCGGTGAGGCCTT
AATTCGTCCGCACCTGGTCGGTATCTGCAACACCGATCTGGAGATCACTCGTGGCTACATGAATTTTCGCGGCGTACTTG
GTCATGAGTTTGTTGGTACCGTTGTCGCCAGTGACGATCCATCCTGGCAGGGGAAGCGCGTCGTCGGAGAAATTAATGCG
GCCTGTCGCCGCTGCCCCACCTGTTTGCGCGGCGATGTCAGCCATTGTCCACAACGCACAACCCTTGGTATCTACCGGCG
TGATGGAGCGATGGCCGACCTGTTCACGTTACCCGAGGCCTGCCTGCACGAAGTGCCTGCCTCGGTGAGTGATGAAGCTG
CGGTCTTCACCGAACCGCTGGCAGCCGCGCTCGAAATCGTCGAACAGAGCCATATTCGTCCAACCGAGCGGGTTGCTGTC
GTAGGTGACGGCAAACTCGGTGCTATGATCGTTCAAGTTCTCCGTCTCACCGGTTGTGAACTCACGCTGATCGGTCGGCA
TCCCGAACGGTGGGACGTTTACCGTCAGCAGGGAATAACCTGTGTGCGCAGTACAGACCTTCCCGGCACCTTATTTGATG
TCGTCGTCGATTGTACCGGTAATCCATCTGGCTTGAATATCGCACGACAAATCATTCGTCCACGTGGTCGATTAATCTTG
AAGAGCACCTTTGCTGCCGAGACTCAGCTTAACCTTAGCATGGTGGTAGTTGATGAGATTCAATTGATTGGCTCGCGCTG
TGGGCCATTTGCGCCCGCATTACGCTTACTAGAACGGGGACTGATTGCCACCACTCCCTTGATCAGTGCCCGTTATCCAC
TTGCTGAAGGTTTACAAGCGTTTGCCGCTGCTCCCGGCCAACTTAAGGTTTTGCTCAGTGTCTGA

Upstream 100 bases:

>100_bases
GGCATCGTAGTTAACTCCTCGCCTTGACTTCATAACATAGCGAGTAGCAGCATATAGTATAATAGAGTTCGGTGTCATTA
AGCGTGAAGACAGCATTGTT

Downstream 100 bases:

>100_bases
AGAAGTTCATCTGCCAACAGTTCTTTTCGCCGACGACGACCCCCATATTCGTACCTTGCTCAGTGATACATTGACGCAAG
CCGGTTTCGCTGTACTGGCT

Product: alcohol dehydrogenase GroES domain-containing protein

Products: NA

Alternate protein names: DOIA dehydrogenase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRAIFFNGDLQYTTDYPDPVRQPGEALIRPHLVGICNTDLEITRGYMNFRGVLGHEFVGTVVASDDPSWQGKRVVGEINA
ACRRCPTCLRGDVSHCPQRTTLGIYRRDGAMADLFTLPEACLHEVPASVSDEAAVFTEPLAAALEIVEQSHIRPTERVAV
VGDGKLGAMIVQVLRLTGCELTLIGRHPERWDVYRQQGITCVRSTDLPGTLFDVVVDCTGNPSGLNIARQIIRPRGRLIL
KSTFAAETQLNLSMVVVDEIQLIGSRCGPFAPALRLLERGLIATTPLISARYPLAEGLQAFAAAPGQLKVLLSV

Sequences:

>Translated_314_residues
MRAIFFNGDLQYTTDYPDPVRQPGEALIRPHLVGICNTDLEITRGYMNFRGVLGHEFVGTVVASDDPSWQGKRVVGEINA
ACRRCPTCLRGDVSHCPQRTTLGIYRRDGAMADLFTLPEACLHEVPASVSDEAAVFTEPLAAALEIVEQSHIRPTERVAV
VGDGKLGAMIVQVLRLTGCELTLIGRHPERWDVYRQQGITCVRSTDLPGTLFDVVVDCTGNPSGLNIARQIIRPRGRLIL
KSTFAAETQLNLSMVVVDEIQLIGSRCGPFAPALRLLERGLIATTPLISARYPLAEGLQAFAAAPGQLKVLLSV
>Mature_314_residues
MRAIFFNGDLQYTTDYPDPVRQPGEALIRPHLVGICNTDLEITRGYMNFRGVLGHEFVGTVVASDDPSWQGKRVVGEINA
ACRRCPTCLRGDVSHCPQRTTLGIYRRDGAMADLFTLPEACLHEVPASVSDEAAVFTEPLAAALEIVEQSHIRPTERVAV
VGDGKLGAMIVQVLRLTGCELTLIGRHPERWDVYRQQGITCVRSTDLPGTLFDVVVDCTGNPSGLNIARQIIRPRGRLIL
KSTFAAETQLNLSMVVVDEIQLIGSRCGPFAPALRLLERGLIATTPLISARYPLAEGLQAFAAAPGQLKVLLSV

Specific function: Catalyzes the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(+) or NADP(+), forming 3-amino-2,3-dideoxy-scyllo- inosose (amino-DOI) [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. DOIA dehydrogenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=319, Percent_Identity=25.0783699059561, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI226510992, Length=300, Percent_Identity=27.6666666666667, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788075, Length=336, Percent_Identity=24.702380952381, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787863, Length=295, Percent_Identity=26.7796610169492, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790718, Length=285, Percent_Identity=25.6140350877193, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790045, Length=330, Percent_Identity=25.4545454545455, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788073, Length=309, Percent_Identity=22.6537216828479, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788407, Length=326, Percent_Identity=23.9263803680982, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17562878, Length=311, Percent_Identity=23.4726688102894, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=329, Percent_Identity=22.1884498480243, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=310, Percent_Identity=22.5806451612903, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=358, Percent_Identity=23.7430167597765, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17137530, Length=328, Percent_Identity=23.1707317073171, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: NA

Molecular weight: Translated: 34197; Mature: 34197

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAIFFNGDLQYTTDYPDPVRQPGEALIRPHLVGICNTDLEITRGYMNFRGVLGHEFVGT
CEEEEECCCEEEECCCCCHHCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEE
VVASDDPSWQGKRVVGEINAACRRCPTCLRGDVSHCPQRTTLGIYRRDGAMADLFTLPEA
EEECCCCCCCCCHHHHHHHHHHHHCCHHHCCCHHHCCCCCEEEEEECCCCHHHHHHCHHH
CLHEVPASVSDEAAVFTEPLAAALEIVEQSHIRPTERVAVVGDGKLGAMIVQVLRLTGCE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCE
LTLIGRHPERWDVYRQQGITCVRSTDLPGTLFDVVVDCTGNPSGLNIARQIIRPRGRLIL
EEEECCCCHHHHHHHHHCCEEEECCCCCCCEEHEEEECCCCCCHHHHHHHHHCCCCCEEE
KSTFAAETQLNLSMVVVDEIQLIGSRCGPFAPALRLLERGLIATTPLISARYPLAEGLQA
EEHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHCCCCHHHHHHH
FAAAPGQLKVLLSV
HHCCCCCEEEEEEC
>Mature Secondary Structure
MRAIFFNGDLQYTTDYPDPVRQPGEALIRPHLVGICNTDLEITRGYMNFRGVLGHEFVGT
CEEEEECCCEEEECCCCCHHCCCCHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHEE
VVASDDPSWQGKRVVGEINAACRRCPTCLRGDVSHCPQRTTLGIYRRDGAMADLFTLPEA
EEECCCCCCCCCHHHHHHHHHHHHCCHHHCCCHHHCCCCCEEEEEECCCCHHHHHHCHHH
CLHEVPASVSDEAAVFTEPLAAALEIVEQSHIRPTERVAVVGDGKLGAMIVQVLRLTGCE
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCE
LTLIGRHPERWDVYRQQGITCVRSTDLPGTLFDVVVDCTGNPSGLNIARQIIRPRGRLIL
EEEECCCCHHHHHHHHHCCEEEECCCCCCCEEHEEEECCCCCCHHHHHHHHHCCCCCEEE
KSTFAAETQLNLSMVVVDEIQLIGSRCGPFAPALRLLERGLIATTPLISARYPLAEGLQA
EEHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCHHHCCCCHHHHHHH
FAAAPGQLKVLLSV
HHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA