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The map label for this gene is ptxB [H]

Identifier: 222523322

GI number: 222523322

Start: 23504

End: 24421

Strand: Direct

Name: ptxB [H]

Synonym: Chy400_0023

Alternate gene names: 222523322

Gene position: 23504-24421 (Clockwise)

Preceding gene: 222523321

Following gene: 222523323

Centisome position: 0.45

GC content: 55.99

Gene sequence:

>918_bases
ATGAAATATTATCTGTTCATCATCTTTATCATACTGCTTGGGATCAGTGGTTGCCAGCGCACGACAACCTCTCTCATCCA
GCTTGACGATCTGCAACCACTGCCAATGCCCACGCCGGTAACGTCACCACCCCTGCGGGTATCGGTGGCATCGGTCATTT
CAGCCCAGGGAACGGTGCAGAGCTACCAACCGTTGCTCGATTACCTGAGCGCCCGGCTTGGCCGTGAAGTAACCTTGATT
CAGCGCCGTACCTACACCGAAACGAACGAGTTGATCGGGCGCAATGAAGTCGATGTTGCGTTTGTCTGCACCAGTGCCTA
CATTGATGGACGTGACCGCTACGGGATGAGTCTGCTGGTTGCTCCCCAGGTCAACGGTGAAACAACGTACCATTCGCTCC
TGATTGTACCGACGGATAGCCCGGCCCGTTCTATGGCCGATCTGCGTGGTAAGGTCTTTGCCTTTACTGATCCAACGTCG
TTTAGCGGGCGCGTCTACCCAACGGCCCTGGTGCAAGAGCTGGGTGATAGTCCAGAGAGCTTTTTCCAGGATACGTTCTT
CACCTACAGTCACGACGCGGCAATCGAAGCAGTGGCTAACGGCCTCGCCGATGGTGCCGCCGTTGATAGTCTGGTGTTCC
ACTTTGCCCTATCCCGGCATCCCGAACTACGTGAGCAGGTAAAAGTGATCCACACCTCACCGCCGTTCGGCATTCCACCG
GTTGTGGTTGGACCGGGAGTCCGGCCTCAGTTGCGTGCTGAGCTGCAAAATATTCTGCTGGGCATGGCTGACGATCCTTC
ACCCCTGGCCCAAACTGCCTTGGAGTCGTTAGGCATTGATCGCTTCGTCATGATTGACGATGCAGCCTACGCCAGTGCCC
GGCTACTACGCTCACAAGTAGGACCGGTATCGCCATGA

Upstream 100 bases:

>100_bases
ATGTGCCGAATGTCCACCAACAGTTACGATCTCTTTCTGTATCAGGCAAGAGGCAAATGATATGATCGCTTTGTCACAAA
GAATGGCATAGTGACGAGTT

Downstream 100 bases:

>100_bases
ACGCAGGGTTCTTGCCACATCAGGTTGTACGATGGGCGACAACGCTATGGCAGATTGTCGGCTCGGTAAATATTCGCGCC
AAAATTCTGGGTATTGTACT

Product: phosphonate ABC transporter periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKYYLFIIFIILLGISGCQRTTTSLIQLDDLQPLPMPTPVTSPPLRVSVASVISAQGTVQSYQPLLDYLSARLGREVTLI
QRRTYTETNELIGRNEVDVAFVCTSAYIDGRDRYGMSLLVAPQVNGETTYHSLLIVPTDSPARSMADLRGKVFAFTDPTS
FSGRVYPTALVQELGDSPESFFQDTFFTYSHDAAIEAVANGLADGAAVDSLVFHFALSRHPELREQVKVIHTSPPFGIPP
VVVGPGVRPQLRAELQNILLGMADDPSPLAQTALESLGIDRFVMIDDAAYASARLLRSQVGPVSP

Sequences:

>Translated_305_residues
MKYYLFIIFIILLGISGCQRTTTSLIQLDDLQPLPMPTPVTSPPLRVSVASVISAQGTVQSYQPLLDYLSARLGREVTLI
QRRTYTETNELIGRNEVDVAFVCTSAYIDGRDRYGMSLLVAPQVNGETTYHSLLIVPTDSPARSMADLRGKVFAFTDPTS
FSGRVYPTALVQELGDSPESFFQDTFFTYSHDAAIEAVANGLADGAAVDSLVFHFALSRHPELREQVKVIHTSPPFGIPP
VVVGPGVRPQLRAELQNILLGMADDPSPLAQTALESLGIDRFVMIDDAAYASARLLRSQVGPVSP
>Mature_305_residues
MKYYLFIIFIILLGISGCQRTTTSLIQLDDLQPLPMPTPVTSPPLRVSVASVISAQGTVQSYQPLLDYLSARLGREVTLI
QRRTYTETNELIGRNEVDVAFVCTSAYIDGRDRYGMSLLVAPQVNGETTYHSLLIVPTDSPARSMADLRGKVFAFTDPTS
FSGRVYPTALVQELGDSPESFFQDTFFTYSHDAAIEAVANGLADGAAVDSLVFHFALSRHPELREQVKVIHTSPPFGIPP
VVVGPGVRPQLRAELQNILLGMADDPSPLAQTALESLGIDRFVMIDDAAYASARLLRSQVGPVSP

Specific function: Probably forms part of a binding-protein-dependent phosphite transporter. Required for oxidation of phosphite to phosphate [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015168
- InterPro:   IPR005770 [H]

Pfam domain/function: PF09084 NMT1 [H]

EC number: NA

Molecular weight: Translated: 33231; Mature: 33231

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYLFIIFIILLGISGCQRTTTSLIQLDDLQPLPMPTPVTSPPLRVSVASVISAQGTVQ
CHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
SYQPLLDYLSARLGREVTLIQRRTYTETNELIGRNEVDVAFVCTSAYIDGRDRYGMSLLV
HHHHHHHHHHHHHCCEEEEEEHHCCCHHHHHCCCCCCCEEEEEEEHEECCCCCCCCEEEE
APQVNGETTYHSLLIVPTDSPARSMADLRGKVFAFTDPTSFSGRVYPTALVQELGDSPES
ECCCCCCCEEEEEEEEECCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHH
FFQDTFFTYSHDAAIEAVANGLADGAAVDSLVFHFALSRHPELREQVKVIHTSPPFGIPP
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHEEEECCCCCCCCC
VVVGPGVRPQLRAELQNILLGMADDPSPLAQTALESLGIDRFVMIDDAAYASARLLRSQV
EEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHC
GPVSP
CCCCC
>Mature Secondary Structure
MKYYLFIIFIILLGISGCQRTTTSLIQLDDLQPLPMPTPVTSPPLRVSVASVISAQGTVQ
CHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHH
SYQPLLDYLSARLGREVTLIQRRTYTETNELIGRNEVDVAFVCTSAYIDGRDRYGMSLLV
HHHHHHHHHHHHHCCEEEEEEHHCCCHHHHHCCCCCCCEEEEEEEHEECCCCCCCCEEEE
APQVNGETTYHSLLIVPTDSPARSMADLRGKVFAFTDPTSFSGRVYPTALVQELGDSPES
ECCCCCCCEEEEEEEEECCCCHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHCCCHHH
FFQDTFFTYSHDAAIEAVANGLADGAAVDSLVFHFALSRHPELREQVKVIHTSPPFGIPP
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHEEEECCCCCCCCC
VVVGPGVRPQLRAELQNILLGMADDPSPLAQTALESLGIDRFVMIDDAAYASARLLRSQV
EEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHC
GPVSP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791102 [H]