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The map label for this gene is pbpB [H]

Identifier: 222151345

GI number: 222151345

Start: 1207003

End: 1209339

Strand: Direct

Name: pbpB [H]

Synonym: MCCL_1098

Alternate gene names: 222151345

Gene position: 1207003-1209339 (Clockwise)

Preceding gene: 222151344

Following gene: 222151355

Centisome position: 57.41

GC content: 37.83

Gene sequence:

>2337_bases
ATGGCTAGAAAGAAGAAACGTAATATAAAAAAGTTAATTCTACAAATTACAGGCGGCATATTTCTTTTATTTGCTGCACT
TTTTATTTGTGGAATGCTTCTTTTCCTATATTATGCATACAAAGCACCAAAGTTTAATGAAGCAGCGCTTCGTGATCAGC
TTCCTACAAAAATATACGACAAGGATGATAACTTAGTTACCGTTCTTCAAATGGGACAAAAACGTGAACAGGTCAAGTTT
AAAGACATTCCTGACCAAGTAAAGAATGCCGTACTCGCAACTGAAGACAATCGTTTTTATGAACATGGCGCTATCGATTT
CAAAAGATTATCTGGTGCAATATTTAAAAATGTCTCCGGAGGTTTCGGGTCACAAGGTGCATCTACTTTAACACAGCAAG
TTGTTAAACGTTCATTTCTGACGGACAAGAAATCTATCGAAAGAAAGGCGCAGGAAGCCTATCTTTCCTACAGATTAGAA
CAAGAATACAGCAAAGATGAAATATTTGAAATGTACCTTAATAAAATTTATTATTCTGACGGTATCTATGGTGTGAAAAC
AGCTGCAAAATATTATTTCAATAAAGATTTAAATCAGCTTTCACTCGCAGAATCAGCATATCTTGCCGGTTTACCTCAGA
TTCCTAACACTTATAATATATATGATAATCCAGATGCTGCTGAAAAGCGCAAGGATACTGTTCTATATTTGATGCATCGT
CACGGCAGAATCACGAAGGCTGAAATGGAAGATGCACAAAATACAGACCTTTCACAGACTTTAGTGCAACGTACAGCCGC
ACAAAGACAGAACCTTGAACAAAATGATCCGGACTATGCATCCTATATTAACGTCATAAAACAAGAAATCAAGAAGAATA
AAGAATTTGAAGGTAAAGATTTGCAAGATATATTAACGAGCGGTTTATCCATCTATACTAACATGGATAACAATGTACAA
TTATCACTTCAGAATAACACTGATAACATGAACATCTACAAGAATAAATATCAACAGGCTGCTTCTTCTATCGTTGATAC
GAAAACAGGTGCACTGGTTGCAATTAGTGGTGGAAGAAACTATACAGATGTTGTCGATAGAAATTTAGCTACAGATGTAC
ATCCAGTTGGTTCAACAATTAAACCGTTTTTAAGCTATGGCCCTATAATCGAGAATGAACAATGGTCCACAGGACATAGA
ATTCAGGATGAGGCTGAATATGATATTCACGGCATTAAATTTAAGAACTATGATTCTCAGAATCATGGTAATGTTACAAT
GAGAGATGCAATAAGACGAAGTTTAAATATACCGGCATTAAAGATATTCCAGACAGTACAGTCTGAGTCAGGAAATGACG
CGCCATATAAATTTGCAAAGAACTTAGGATTAAATTATCCATCCAGCGACCTAGGTCCTTCAGACGCATTAGGGGGATCA
TCTTCTGAGTTCACTCCTTTACAGATGGCCAGTGCCTATGCTTCATTCGGTAATGGCGGAACATATAATGAATCTCATGC
GATTCGAAAGATTGTAACACAAGATAATGAAACAATAAAATTTGATTTTACTTCTAGAAAAGCAATGGAAGATTATACAG
CATATATGATTACTGATATGTTAAAAGGGACATTTGAACCATATGGTACTGCTTATGGTAGTCAAATCCCTGGTTTAAAC
CTTGCTGCGAAGACAGGAACAGGAACGTATGGCGATGAAACTTACGAAAAATATAATTTACCTGATAATGCAGCAAAAGA
TGTCTGGATTGTAGGGTATTCACCACAATATACGATGTCAGTGTGGATGGGTTTCACACAAATGGCACAATATGGAGAGA
ATTCTTTTGTAGGCTCTGAAGAACAATCACTTCCAAAAGTACTTTTCAGACAAGTTATGAGTGAAATCAGTCCTATTGAT
GGTCAAGATTTTGAAATGCCTTCCTCGGTGGAAAAATATGGTGGCTTTCTACAGGTTAAAGGACAATCAGATAATGGATA
TACGAGTAACATAAAACCAAATAATACAAATGATGCTGTTCGGGATAATTCTCGGGCAGCTTCTACAAATATTTATCCAG
ATGATACGAACGATAATTCGACAAGTGAACGCAACACAACTGAAGCTCGTACAGTAGAAAGTACGACGACTGAACGTCCA
ACAACTGAACGTGCAACAACTGAACGTCCGACGACTGAACGTCCGACAACTGAACGTGCAACAACTGAACGCCCGACTAC
ACAACCTGCTACGACCGAGCAAGCAACAACTGAGCGGGCAACAACGCAACCTGCTACAACTGAGCGGGCAACACAGCCTG
CTGCAAGTCAGCCATAA

Upstream 100 bases:

>100_bases
CGGTTATCCTGTGGAAAAAAAATATCAGCCTAGAATTGACTATTTAAAAGTTATAGACCACATATATTTTAATGAATAAA
TCGAGAGTGAGGAAATTTTA

Downstream 100 bases:

>100_bases
TTAAATACGACTATATTATATTAAGCCCTTATTTCATCTCAATGCGTTGATTGAATGAAATAAGGGCTTCTTTTTATTTT
TTCTTCTTCAAATCATGGAC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 778; Mature: 777

Protein sequence:

>778_residues
MARKKKRNIKKLILQITGGIFLLFAALFICGMLLFLYYAYKAPKFNEAALRDQLPTKIYDKDDNLVTVLQMGQKREQVKF
KDIPDQVKNAVLATEDNRFYEHGAIDFKRLSGAIFKNVSGGFGSQGASTLTQQVVKRSFLTDKKSIERKAQEAYLSYRLE
QEYSKDEIFEMYLNKIYYSDGIYGVKTAAKYYFNKDLNQLSLAESAYLAGLPQIPNTYNIYDNPDAAEKRKDTVLYLMHR
HGRITKAEMEDAQNTDLSQTLVQRTAAQRQNLEQNDPDYASYINVIKQEIKKNKEFEGKDLQDILTSGLSIYTNMDNNVQ
LSLQNNTDNMNIYKNKYQQAASSIVDTKTGALVAISGGRNYTDVVDRNLATDVHPVGSTIKPFLSYGPIIENEQWSTGHR
IQDEAEYDIHGIKFKNYDSQNHGNVTMRDAIRRSLNIPALKIFQTVQSESGNDAPYKFAKNLGLNYPSSDLGPSDALGGS
SSEFTPLQMASAYASFGNGGTYNESHAIRKIVTQDNETIKFDFTSRKAMEDYTAYMITDMLKGTFEPYGTAYGSQIPGLN
LAAKTGTGTYGDETYEKYNLPDNAAKDVWIVGYSPQYTMSVWMGFTQMAQYGENSFVGSEEQSLPKVLFRQVMSEISPID
GQDFEMPSSVEKYGGFLQVKGQSDNGYTSNIKPNNTNDAVRDNSRAASTNIYPDDTNDNSTSERNTTEARTVESTTTERP
TTERATTERPTTERPTTERATTERPTTQPATTEQATTERATTQPATTERATQPAASQP

Sequences:

>Translated_778_residues
MARKKKRNIKKLILQITGGIFLLFAALFICGMLLFLYYAYKAPKFNEAALRDQLPTKIYDKDDNLVTVLQMGQKREQVKF
KDIPDQVKNAVLATEDNRFYEHGAIDFKRLSGAIFKNVSGGFGSQGASTLTQQVVKRSFLTDKKSIERKAQEAYLSYRLE
QEYSKDEIFEMYLNKIYYSDGIYGVKTAAKYYFNKDLNQLSLAESAYLAGLPQIPNTYNIYDNPDAAEKRKDTVLYLMHR
HGRITKAEMEDAQNTDLSQTLVQRTAAQRQNLEQNDPDYASYINVIKQEIKKNKEFEGKDLQDILTSGLSIYTNMDNNVQ
LSLQNNTDNMNIYKNKYQQAASSIVDTKTGALVAISGGRNYTDVVDRNLATDVHPVGSTIKPFLSYGPIIENEQWSTGHR
IQDEAEYDIHGIKFKNYDSQNHGNVTMRDAIRRSLNIPALKIFQTVQSESGNDAPYKFAKNLGLNYPSSDLGPSDALGGS
SSEFTPLQMASAYASFGNGGTYNESHAIRKIVTQDNETIKFDFTSRKAMEDYTAYMITDMLKGTFEPYGTAYGSQIPGLN
LAAKTGTGTYGDETYEKYNLPDNAAKDVWIVGYSPQYTMSVWMGFTQMAQYGENSFVGSEEQSLPKVLFRQVMSEISPID
GQDFEMPSSVEKYGGFLQVKGQSDNGYTSNIKPNNTNDAVRDNSRAASTNIYPDDTNDNSTSERNTTEARTVESTTTERP
TTERATTERPTTERPTTERATTERPTTQPATTEQATTERATTQPATTERATQPAASQP
>Mature_777_residues
ARKKKRNIKKLILQITGGIFLLFAALFICGMLLFLYYAYKAPKFNEAALRDQLPTKIYDKDDNLVTVLQMGQKREQVKFK
DIPDQVKNAVLATEDNRFYEHGAIDFKRLSGAIFKNVSGGFGSQGASTLTQQVVKRSFLTDKKSIERKAQEAYLSYRLEQ
EYSKDEIFEMYLNKIYYSDGIYGVKTAAKYYFNKDLNQLSLAESAYLAGLPQIPNTYNIYDNPDAAEKRKDTVLYLMHRH
GRITKAEMEDAQNTDLSQTLVQRTAAQRQNLEQNDPDYASYINVIKQEIKKNKEFEGKDLQDILTSGLSIYTNMDNNVQL
SLQNNTDNMNIYKNKYQQAASSIVDTKTGALVAISGGRNYTDVVDRNLATDVHPVGSTIKPFLSYGPIIENEQWSTGHRI
QDEAEYDIHGIKFKNYDSQNHGNVTMRDAIRRSLNIPALKIFQTVQSESGNDAPYKFAKNLGLNYPSSDLGPSDALGGSS
SEFTPLQMASAYASFGNGGTYNESHAIRKIVTQDNETIKFDFTSRKAMEDYTAYMITDMLKGTFEPYGTAYGSQIPGLNL
AAKTGTGTYGDETYEKYNLPDNAAKDVWIVGYSPQYTMSVWMGFTQMAQYGENSFVGSEEQSLPKVLFRQVMSEISPIDG
QDFEMPSSVEKYGGFLQVKGQSDNGYTSNIKPNNTNDAVRDNSRAASTNIYPDDTNDNSTSERNTTEARTVESTTTERPT
TERATTERPTTERPTTERATTERPTTQPATTEQATTERATTQPATTERATQPAASQP

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=567, Percent_Identity=26.1022927689594, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI87082258, Length=382, Percent_Identity=32.1989528795811, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1788867, Length=609, Percent_Identity=24.9589490968801, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789601, Length=153, Percent_Identity=34.640522875817, Blast_Score=93, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 87280; Mature: 87149

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKKRNIKKLILQITGGIFLLFAALFICGMLLFLYYAYKAPKFNEAALRDQLPTKIYD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEC
KDDNLVTVLQMGQKREQVKFKDIPDQVKNAVLATEDNRFYEHGAIDFKRLSGAIFKNVSG
CCCCEEEEHHHCCHHHCCCCCCCCHHHHCCEEECCCCCCHHCCCCHHHHHHHHHHHHCCC
GFGSQGASTLTQQVVKRSFLTDKKSIERKAQEAYLSYRLEQEYSKDEIFEMYLNKIYYSD
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GIYGVKTAAKYYFNKDLNQLSLAESAYLAGLPQIPNTYNIYDNPDAAEKRKDTVLYLMHR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHCCEEEEEHH
HGRITKAEMEDAQNTDLSQTLVQRTAAQRQNLEQNDPDYASYINVIKQEIKKNKEFEGKD
CCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
LQDILTSGLSIYTNMDNNVQLSLQNNTDNMNIYKNKYQQAASSIVDTKTGALVAISGGRN
HHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
YTDVVDRNLATDVHPVGSTIKPFLSYGPIIENEQWSTGHRIQDEAEYDIHGIKFKNYDSQ
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
NHGNVTMRDAIRRSLNIPALKIFQTVQSESGNDAPYKFAKNLGLNYPSSDLGPSDALGGS
CCCCEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
SSEFTPLQMASAYASFGNGGTYNESHAIRKIVTQDNETIKFDFTSRKAMEDYTAYMITDM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
LKGTFEPYGTAYGSQIPGLNLAAKTGTGTYGDETYEKYNLPDNAAKDVWIVGYSPQYTMS
HHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCHHHH
VWMGFTQMAQYGENSFVGSEEQSLPKVLFRQVMSEISPIDGQDFEMPSSVEKYGGFLQVK
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEE
GQSDNGYTSNIKPNNTNDAVRDNSRAASTNIYPDDTNDNSTSERNTTEARTVESTTTERP
ECCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
TTERATTERPTTERPTTERATTERPTTQPATTEQATTERATTQPATTERATQPAASQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
ARKKKRNIKKLILQITGGIFLLFAALFICGMLLFLYYAYKAPKFNEAALRDQLPTKIYD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEC
KDDNLVTVLQMGQKREQVKFKDIPDQVKNAVLATEDNRFYEHGAIDFKRLSGAIFKNVSG
CCCCEEEEHHHCCHHHCCCCCCCCHHHHCCEEECCCCCCHHCCCCHHHHHHHHHHHHCCC
GFGSQGASTLTQQVVKRSFLTDKKSIERKAQEAYLSYRLEQEYSKDEIFEMYLNKIYYSD
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
GIYGVKTAAKYYFNKDLNQLSLAESAYLAGLPQIPNTYNIYDNPDAAEKRKDTVLYLMHR
CCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHCCEEEEEHH
HGRITKAEMEDAQNTDLSQTLVQRTAAQRQNLEQNDPDYASYINVIKQEIKKNKEFEGKD
CCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
LQDILTSGLSIYTNMDNNVQLSLQNNTDNMNIYKNKYQQAASSIVDTKTGALVAISGGRN
HHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
YTDVVDRNLATDVHPVGSTIKPFLSYGPIIENEQWSTGHRIQDEAEYDIHGIKFKNYDSQ
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
NHGNVTMRDAIRRSLNIPALKIFQTVQSESGNDAPYKFAKNLGLNYPSSDLGPSDALGGS
CCCCEEHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCC
SSEFTPLQMASAYASFGNGGTYNESHAIRKIVTQDNETIKFDFTSRKAMEDYTAYMITDM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHH
LKGTFEPYGTAYGSQIPGLNLAAKTGTGTYGDETYEKYNLPDNAAKDVWIVGYSPQYTMS
HHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCEEEEECCCCHHHH
VWMGFTQMAQYGENSFVGSEEQSLPKVLFRQVMSEISPIDGQDFEMPSSVEKYGGFLQVK
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCEEEEE
GQSDNGYTSNIKPNNTNDAVRDNSRAASTNIYPDDTNDNSTSERNTTEARTVESTTTERP
ECCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCC
TTERATTERPTTERPTTERATTERPTTQPATTEQATTERATTQPATTERATQPAASQP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]