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The map label for this gene is opuD [H]

Identifier: 222151254

GI number: 222151254

Start: 1108341

End: 1109891

Strand: Direct

Name: opuD [H]

Synonym: MCCL_1007

Alternate gene names: 222151254

Gene position: 1108341-1109891 (Clockwise)

Preceding gene: 222151252

Following gene: 222151255

Centisome position: 52.72

GC content: 36.62

Gene sequence:

>1551_bases
ATGACATCAGGAAAAAAATTGACACCTGTATTTTTTATTGCGTTATCACTTGTAATCATTACTGTTTTGTTAGGTGTAAT
AAGTCCATCAGGATTTGGTGATGTTACGAGCCAGATTAATGTATTTATTACAAAATATTTCGGTTGGTATTATGTAATGA
TTACATCTGTATTTGTATTTGCCTGCTTGTTTCTAATTCTTAGCCCTATCGGTAAACTAAAATTAGGTAAACCATCAGAT
AAACCAGAATTTAGTACAATATCCTGGTTTGCGATGTTATTTAGCGCAGGTATGGGTATCGGACTCGTATTCTATGGTGC
TGCAGAACCATTAAGTCACTTTATAAAATCACCGACAGCTGATGCTCAGTCAGATGAAGCGATGAAAGATGCATTTCGCT
ATACGTTCTTTCATTGGGGATTTCATGCATGGGCAACATACGGAATAGTCGCTTTAGGTCTTGCATACGCTCAATTCAGG
AAAGGGGAAGCGGGATTATTATCGAAGACATTAAGACCATTGCTCGGCGATAGAGTAGATGGTGTTCTGGGAACAATCAT
TGATGTACTTGCAGTATTTGCAACTGTAGTTGGGGTTGCAGTATCATTAGGAATGGGTGCTCAGCAGATCAATGGTGGTT
TACATTTCTTATTTGATGTACCAAATACAATTAATGTAAAGTTAATAATCATTGTCATTGTTACTATATTATTCTTAATG
AGTGCGTGGAGCGGCTTATCAAAAGGGATACAGATTTTAAGTAATATTAATATAGGTATCGCAACATTATTACTGGTTGC
AGTTCTCTTTTTAGGTCCAACAGTGCTGATTATGAATATGTTTTCAACATCGCTAGGGAATTTATTACAGAACTTCTTGT
CTATGAGTTTTGATGCTGCACCTTTAGATGAGTCGAAGCGTGAGTGGATGAACAGCTGGACAATCTACTACTGGGGTTGG
TGGATGAGCTGGAGTCCATTCGTTGGAATATTCATAGCACGTGTCTCTAAAGGACGCACTATTCGTGAATTTTTAATCGG
TGTACTACTCGTTCCGACAATTATCAGCACACTTTGGTTTAGTGTGTTTGGTGTAAGTGGAATACAGATATTTAAAAAGT
TCCCTGAAATTGGTAAACTTGCTGTTGAAACACAGCTATTCGCAATATTTGAACATATGCCTATGGCTATAGGATTATCA
TTATTGGCATTGCTATTAATTTGTACTTTCTTTATCACTTCAGCAGATTCAGCAACATTTGTACTAGGAATGCAATCTAC
AAATGGGTCACTTGAACCAGCAAACTATATTAAAGTGATATGGGGTGTCGCACAGTCAATTATTGCTGCAGTGCTTTTAT
ATGCAGGTGGACTGAGCGCTTTACAAAGTGCCGCGATTATCGCAGCTTTTCCATTTTCATTTGTACTTATTCTGATGCTG
TTATCTTTATATAAAGATGGTAATGCAGAACGTATGAGAATAGGGCTGACGTTAAAACCTGATAAGAAATTCAGAAAATC
ATATCAAGAAAATCAAAATAACCAAAAATAA

Upstream 100 bases:

>100_bases
GAGAACTAGATTTTGAAAAAACGTTTCTATTTGTGTAATTAATTATTGTTTGTTATACTTAGAACTGTTTTTTAAAATTT
TAATTCGGAAGGAGTATTTT

Downstream 100 bases:

>100_bases
TATATTAAAATACCTGTCGACCACGCAAATCAACTGTGAATTGTCGACAGGTATTTTTTATAAGTAAAACTTATAACTAA
GTATAGCTAATTTGGAATGT

Product: glycine/betaine ABC transporter

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTSGKKLTPVFFIALSLVIITVLLGVISPSGFGDVTSQINVFITKYFGWYYVMITSVFVFACLFLILSPIGKLKLGKPSD
KPEFSTISWFAMLFSAGMGIGLVFYGAAEPLSHFIKSPTADAQSDEAMKDAFRYTFFHWGFHAWATYGIVALGLAYAQFR
KGEAGLLSKTLRPLLGDRVDGVLGTIIDVLAVFATVVGVAVSLGMGAQQINGGLHFLFDVPNTINVKLIIIVIVTILFLM
SAWSGLSKGIQILSNINIGIATLLLVAVLFLGPTVLIMNMFSTSLGNLLQNFLSMSFDAAPLDESKREWMNSWTIYYWGW
WMSWSPFVGIFIARVSKGRTIREFLIGVLLVPTIISTLWFSVFGVSGIQIFKKFPEIGKLAVETQLFAIFEHMPMAIGLS
LLALLLICTFFITSADSATFVLGMQSTNGSLEPANYIKVIWGVAQSIIAAVLLYAGGLSALQSAAIIAAFPFSFVLILML
LSLYKDGNAERMRIGLTLKPDKKFRKSYQENQNNQK

Sequences:

>Translated_516_residues
MTSGKKLTPVFFIALSLVIITVLLGVISPSGFGDVTSQINVFITKYFGWYYVMITSVFVFACLFLILSPIGKLKLGKPSD
KPEFSTISWFAMLFSAGMGIGLVFYGAAEPLSHFIKSPTADAQSDEAMKDAFRYTFFHWGFHAWATYGIVALGLAYAQFR
KGEAGLLSKTLRPLLGDRVDGVLGTIIDVLAVFATVVGVAVSLGMGAQQINGGLHFLFDVPNTINVKLIIIVIVTILFLM
SAWSGLSKGIQILSNINIGIATLLLVAVLFLGPTVLIMNMFSTSLGNLLQNFLSMSFDAAPLDESKREWMNSWTIYYWGW
WMSWSPFVGIFIARVSKGRTIREFLIGVLLVPTIISTLWFSVFGVSGIQIFKKFPEIGKLAVETQLFAIFEHMPMAIGLS
LLALLLICTFFITSADSATFVLGMQSTNGSLEPANYIKVIWGVAQSIIAAVLLYAGGLSALQSAAIIAAFPFSFVLILML
LSLYKDGNAERMRIGLTLKPDKKFRKSYQENQNNQK
>Mature_515_residues
TSGKKLTPVFFIALSLVIITVLLGVISPSGFGDVTSQINVFITKYFGWYYVMITSVFVFACLFLILSPIGKLKLGKPSDK
PEFSTISWFAMLFSAGMGIGLVFYGAAEPLSHFIKSPTADAQSDEAMKDAFRYTFFHWGFHAWATYGIVALGLAYAQFRK
GEAGLLSKTLRPLLGDRVDGVLGTIIDVLAVFATVVGVAVSLGMGAQQINGGLHFLFDVPNTINVKLIIIVIVTILFLMS
AWSGLSKGIQILSNINIGIATLLLVAVLFLGPTVLIMNMFSTSLGNLLQNFLSMSFDAAPLDESKREWMNSWTIYYWGWW
MSWSPFVGIFIARVSKGRTIREFLIGVLLVPTIISTLWFSVFGVSGIQIFKKFPEIGKLAVETQLFAIFEHMPMAIGLSL
LALLLICTFFITSADSATFVLGMQSTNGSLEPANYIKVIWGVAQSIIAAVLLYAGGLSALQSAAIIAAFPFSFVLILMLL
SLYKDGNAERMRIGLTLKPDKKFRKSYQENQNNQK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=459, Percent_Identity=35.7298474945534, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI1786224, Length=492, Percent_Identity=29.8780487804878, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788102, Length=465, Percent_Identity=30.5376344086021, Blast_Score=198, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56608; Mature: 56477

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TSGKKLTPVFFIALSLVIITVLLGVISPSGFGDVTSQINVFITKYFGWYYVMITSVFVF
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]