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The map label for this gene is proS

Identifier: 222151097

GI number: 222151097

Start: 924436

End: 926142

Strand: Direct

Name: proS

Synonym: MCCL_0848

Alternate gene names: 222151097

Gene position: 924436-926142 (Clockwise)

Preceding gene: 222151096

Following gene: 222151098

Centisome position: 43.97

GC content: 40.25

Gene sequence:

>1707_bases
ATGAAACAGAGTAAAATGTTTATTCCAACATTAAGAGAAGTTCCAAGCGATGCAGATAGCAAGAGTCATCAGCTACTGCT
TAAAGCAGGGATGATCAAACAAGTCGCAAGTGGTGTGTATAGTTACTTACCGATTGCTAAACGCGTATTAAATAAAATCG
AGTCAATTGTCCGTGAAGAGATGGAAGCGATTGATGGTGTAGAAATATTAATGCCTGCCTTACAGCCCTCAGAGCTTTGG
AGCGAGTCTGGTCGCTGGCAAAGTTATGGCGCAGAGCTTATGCGTATGACAGACCGTCATGGTCGTGAATTTGCGCTCGG
ACCAACACATGAAGAAATTATTACATCACTAGTGCGTGATGAATTAAAGTCTTATAAGAAGTTACCGGTAACATTATTCC
AGATTCAAAACAAGTTTCGAGATGAGAAGCGTCCTAGATTCGGATTGTTACGTGGCCGTGAATTCATTATGAAGGACGCA
TATAGCTTCCATGCAACAGAAGCGTCACTTGATGAGACATATCAGGATATGTATAATGCATATTCGAAGATTTTCTCGCG
TCTAAACTTACAGTTCCGTCCAGTTATCGCAGATAGTGGTGCAATTGGTGGAAGCCACACGCATGAATTTATGGCGTTAG
CGGAAATTGGTGAAGATACGATCTGTTATACGGATGGCAGTGATTATGCAGCAAATATCGAAAAAGCAGAAGTCGTATAC
CATCCAAATAAGAAACATACAGAAGAGAACGTATTAGAAAAAGTACATACGCCTGGCGTAAAAACAGCGCAGCAGTTAGC
AGATTTCTTGAAGCGTGACTTAGATGAAATTGTGAAATCAATGATTGTTAAAGTCGATGACCAGTTTGTAATGTTCTTAA
TTCGTGGACATCATGAATTAAATGATATTAAGGTAAAATCATTCTTTGGAACAGAGCATGTAGAGATGGCAACAGACGAT
GATATTCGTTCAATCCTAGATGCGAGTCCAGGTTCATTAGGCCCGGTAGGAGTTAATAAGATTGATATCTATGCAGATAA
CAGTGTACAGGATCTCAATAATTTAGCAGTCGGTGCAAATGAAGATGATTATCATTATGTGAATGCGAATGTAGATCGTG
ACTTTAACGTGAAAGCCTATGGTGATTTCCGATTTATTTTAGAAGGTGAACCAGCTGCAGATGGTAGTGGTCCAGTTAAA
TTTGCAGAAGGTATTGAAATCGGACAAGTATTTAAACTCGGAACAAAGTACAGTGAGAGCATGAATGCGACTTTCTTAAA
CGATCAAGGCCGAGCAGAGCCTATGATAATGGGATGTTACGGAATAGGCGTTTCACGTACATTATCAGCAGTAATCGAGC
AGCATAATGATGACAAAGGTATTATATGGCCAACTGCTATTACGCCATATGAAGTGCATATCATCAGTGTGAATCCTAAA
CAGGAAGTCCAAAAAAACCTTGCAGACCAGCTCTATGATACATATCGCACGCAATATGAAGTACTATATGATGACCGAGC
AGAACGTGCGGGTGTGAAATTTAATGATGCAGATTTAATCGGAATACCGGTTCGTGTGGTGGTTGGGAAACAAGCAGCCG
ACGGTATTGTCGAGGTTAAGAACCGTCGTACTGGGGACGCTGTTGAAGTCCATATAGACAACTTACAAGAAACGATTCAA
TCAATTTATGCATCTTTTGAATCATAA

Upstream 100 bases:

>100_bases
GATACTTCTTAAAATAAACAGCACAGTGCTGTTTATTTTTTGTTTGATTAATGAATATTTTATTATATGATATATGTATT
ATAGATTTTAGGAGGCAGCT

Downstream 100 bases:

>100_bases
TTTGATATAATGAATAATAAAAGTAATACGCACCTGCGTGTTACTTTTTCATTTTGAAGGTGGTAAAAAACGTGACAATA
AATAATAACGAAAAATTTAA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MKQSKMFIPTLREVPSDADSKSHQLLLKAGMIKQVASGVYSYLPIAKRVLNKIESIVREEMEAIDGVEILMPALQPSELW
SESGRWQSYGAELMRMTDRHGREFALGPTHEEIITSLVRDELKSYKKLPVTLFQIQNKFRDEKRPRFGLLRGREFIMKDA
YSFHATEASLDETYQDMYNAYSKIFSRLNLQFRPVIADSGAIGGSHTHEFMALAEIGEDTICYTDGSDYAANIEKAEVVY
HPNKKHTEENVLEKVHTPGVKTAQQLADFLKRDLDEIVKSMIVKVDDQFVMFLIRGHHELNDIKVKSFFGTEHVEMATDD
DIRSILDASPGSLGPVGVNKIDIYADNSVQDLNNLAVGANEDDYHYVNANVDRDFNVKAYGDFRFILEGEPAADGSGPVK
FAEGIEIGQVFKLGTKYSESMNATFLNDQGRAEPMIMGCYGIGVSRTLSAVIEQHNDDKGIIWPTAITPYEVHIISVNPK
QEVQKNLADQLYDTYRTQYEVLYDDRAERAGVKFNDADLIGIPVRVVVGKQAADGIVEVKNRRTGDAVEVHIDNLQETIQ
SIYASFES

Sequences:

>Translated_568_residues
MKQSKMFIPTLREVPSDADSKSHQLLLKAGMIKQVASGVYSYLPIAKRVLNKIESIVREEMEAIDGVEILMPALQPSELW
SESGRWQSYGAELMRMTDRHGREFALGPTHEEIITSLVRDELKSYKKLPVTLFQIQNKFRDEKRPRFGLLRGREFIMKDA
YSFHATEASLDETYQDMYNAYSKIFSRLNLQFRPVIADSGAIGGSHTHEFMALAEIGEDTICYTDGSDYAANIEKAEVVY
HPNKKHTEENVLEKVHTPGVKTAQQLADFLKRDLDEIVKSMIVKVDDQFVMFLIRGHHELNDIKVKSFFGTEHVEMATDD
DIRSILDASPGSLGPVGVNKIDIYADNSVQDLNNLAVGANEDDYHYVNANVDRDFNVKAYGDFRFILEGEPAADGSGPVK
FAEGIEIGQVFKLGTKYSESMNATFLNDQGRAEPMIMGCYGIGVSRTLSAVIEQHNDDKGIIWPTAITPYEVHIISVNPK
QEVQKNLADQLYDTYRTQYEVLYDDRAERAGVKFNDADLIGIPVRVVVGKQAADGIVEVKNRRTGDAVEVHIDNLQETIQ
SIYASFES
>Mature_568_residues
MKQSKMFIPTLREVPSDADSKSHQLLLKAGMIKQVASGVYSYLPIAKRVLNKIESIVREEMEAIDGVEILMPALQPSELW
SESGRWQSYGAELMRMTDRHGREFALGPTHEEIITSLVRDELKSYKKLPVTLFQIQNKFRDEKRPRFGLLRGREFIMKDA
YSFHATEASLDETYQDMYNAYSKIFSRLNLQFRPVIADSGAIGGSHTHEFMALAEIGEDTICYTDGSDYAANIEKAEVVY
HPNKKHTEENVLEKVHTPGVKTAQQLADFLKRDLDEIVKSMIVKVDDQFVMFLIRGHHELNDIKVKSFFGTEHVEMATDD
DIRSILDASPGSLGPVGVNKIDIYADNSVQDLNNLAVGANEDDYHYVNANVDRDFNVKAYGDFRFILEGEPAADGSGPVK
FAEGIEIGQVFKLGTKYSESMNATFLNDQGRAEPMIMGCYGIGVSRTLSAVIEQHNDDKGIIWPTAITPYEVHIISVNPK
QEVQKNLADQLYDTYRTQYEVLYDDRAERAGVKFNDADLIGIPVRVVVGKQAADGIVEVKNRRTGDAVEVHIDNLQETIQ
SIYASFES

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=230, Percent_Identity=44.3478260869565, Blast_Score=222, Evalue=7e-58,
Organism=Escherichia coli, GI1786392, Length=556, Percent_Identity=48.5611510791367, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI115532348, Length=213, Percent_Identity=37.0892018779343, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=33.7078651685393, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=201, Percent_Identity=39.3034825870647, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656200, Length=249, Percent_Identity=37.3493975903614, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_MACCJ (B9EBE4)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560251.1
- GeneID:   7389958
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0848
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63916; Mature: 63916

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKMFIPTLREVPSDADSKSHQLLLKAGMIKQVASGVYSYLPIAKRVLNKIESIVREE
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEAIDGVEILMPALQPSELWSESGRWQSYGAELMRMTDRHGREFALGPTHEEIITSLVRD
HHHHCCHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHH
ELKSYKKLPVTLFQIQNKFRDEKRPRFGLLRGREFIMKDAYSFHATEASLDETYQDMYNA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRLNLQFRPVIADSGAIGGSHTHEFMALAEIGEDTICYTDGSDYAANIEKAEVVY
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEE
HPNKKHTEENVLEKVHTPGVKTAQQLADFLKRDLDEIVKSMIVKVDDQFVMFLIRGHHEL
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
NDIKVKSFFGTEHVEMATDDDIRSILDASPGSLGPVGVNKIDIYADNSVQDLNNLAVGAN
CCEEEEEECCCCCEEECCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCC
EDDYHYVNANVDRDFNVKAYGDFRFILEGEPAADGSGPVKFAEGIEIGQVFKLGTKYSES
CCCCEEEECCCCCCCCEEEEECEEEEEECCCCCCCCCCEEHHCCCCHHHHHHHCCHHCCC
MNATFLNDQGRAEPMIMGCYGIGVSRTLSAVIEQHNDDKGIIWPTAITPYEVHIISVNPK
CCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCH
QEVQKNLADQLYDTYRTQYEVLYDDRAERAGVKFNDADLIGIPVRVVVGKQAADGIVEVK
HHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCEECCCCEEECCEEEEECCCHHCCHHHHC
NRRTGDAVEVHIDNLQETIQSIYASFES
CCCCCCEEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQSKMFIPTLREVPSDADSKSHQLLLKAGMIKQVASGVYSYLPIAKRVLNKIESIVREE
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MEAIDGVEILMPALQPSELWSESGRWQSYGAELMRMTDRHGREFALGPTHEEIITSLVRD
HHHHCCHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHH
ELKSYKKLPVTLFQIQNKFRDEKRPRFGLLRGREFIMKDAYSFHATEASLDETYQDMYNA
HHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRLNLQFRPVIADSGAIGGSHTHEFMALAEIGEDTICYTDGSDYAANIEKAEVVY
HHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEE
HPNKKHTEENVLEKVHTPGVKTAQQLADFLKRDLDEIVKSMIVKVDDQFVMFLIRGHHEL
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCC
NDIKVKSFFGTEHVEMATDDDIRSILDASPGSLGPVGVNKIDIYADNSVQDLNNLAVGAN
CCEEEEEECCCCCEEECCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCC
EDDYHYVNANVDRDFNVKAYGDFRFILEGEPAADGSGPVKFAEGIEIGQVFKLGTKYSES
CCCCEEEECCCCCCCCEEEEECEEEEEECCCCCCCCCCEEHHCCCCHHHHHHHCCHHCCC
MNATFLNDQGRAEPMIMGCYGIGVSRTLSAVIEQHNDDKGIIWPTAITPYEVHIISVNPK
CCCEEECCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCH
QEVQKNLADQLYDTYRTQYEVLYDDRAERAGVKFNDADLIGIPVRVVVGKQAADGIVEVK
HHHHHHHHHHHHHHHHHHEEEEECCCHHHCCCEECCCCEEECCEEEEECCCHHCCHHHHC
NRRTGDAVEVHIDNLQETIQSIYASFES
CCCCCCEEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA