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The map label for this gene is trmD

Identifier: 222151072

GI number: 222151072

Start: 896668

End: 897390

Strand: Direct

Name: trmD

Synonym: MCCL_0823

Alternate gene names: 222151072

Gene position: 896668-897390 (Clockwise)

Preceding gene: 222151071

Following gene: 222151073

Centisome position: 42.65

GC content: 38.87

Gene sequence:

>723_bases
ATGAAGATTGATTATTTAACATTATTCCCTGAAATGTTTGACGTACTGAATCATTCTATTATGAAACGTGCACAGGAAAA
AGGAATTGTAGAGCTGAACACAGTGAACTTCAGAGATTATTCAGGTAATAAACATAACCAGGTGGATGATTATCCATATG
GCGGAGGGCAAGGTATGGTTCTAAAGCCAGAACCGATATTCAATGCAATGAATGCCATTGATAAAACGAGCAAGACACGT
GTTATCCTTATGTGTCCGCAAGGAAAACCTTTCACGCAGCAAGTTGCTGAATCGTTAGCACAAGAAGAGCACCTTGTATT
TATTTGTGGTCATTATGAAGGATATGATGAACGCATACGCGAGCATCTTGTAACTGATGAACTCTCTATCGGTGATTATG
TATTAACCGGTGGAGAACTTGCAGCAATTACAATGACAGATGCAATCGTGAGACTGATCCCTGAATCAATAAAAGAAGAA
AGTCATCTGGATGATTCATTCTCAACTGGATTACTTGAATACCCACAATATACGAGACCTGCTGACTATAATGGAATGAA
AGTACCGGATGTACTGCTTAGCGGTAACCATAAGCATATCGAATCATGGCGCCATGAAATGCGCTTGAAGCGTACTTTTG
AACGCAGACCAGATTTATTAGAACACTACCCACTGACTCAGTCAGATAAAGAATATTTAGAACATCTTAAACGTGATAAA
TAG

Upstream 100 bases:

>100_bases
GAGATAAAGAATACTTGATTCCGTATATTGAAGATGTTGTTAAGTCAATCGATATTGATAATAAAAAAATCATCATCGAA
GCGATCGAAGGGTTATTATA

Downstream 100 bases:

>100_bases
ATGTCAATATATATTGCTTTACACAGTGAAATATGATATTATTTTAAAAGTGTGAATCACACATAAATCACTATGATCCG
CTGAGCGAAGACTTAAGAAC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKIDYLTLFPEMFDVLNHSIMKRAQEKGIVELNTVNFRDYSGNKHNQVDDYPYGGGQGMVLKPEPIFNAMNAIDKTSKTR
VILMCPQGKPFTQQVAESLAQEEHLVFICGHYEGYDERIREHLVTDELSIGDYVLTGGELAAITMTDAIVRLIPESIKEE
SHLDDSFSTGLLEYPQYTRPADYNGMKVPDVLLSGNHKHIESWRHEMRLKRTFERRPDLLEHYPLTQSDKEYLEHLKRDK

Sequences:

>Translated_240_residues
MKIDYLTLFPEMFDVLNHSIMKRAQEKGIVELNTVNFRDYSGNKHNQVDDYPYGGGQGMVLKPEPIFNAMNAIDKTSKTR
VILMCPQGKPFTQQVAESLAQEEHLVFICGHYEGYDERIREHLVTDELSIGDYVLTGGELAAITMTDAIVRLIPESIKEE
SHLDDSFSTGLLEYPQYTRPADYNGMKVPDVLLSGNHKHIESWRHEMRLKRTFERRPDLLEHYPLTQSDKEYLEHLKRDK
>Mature_240_residues
MKIDYLTLFPEMFDVLNHSIMKRAQEKGIVELNTVNFRDYSGNKHNQVDDYPYGGGQGMVLKPEPIFNAMNAIDKTSKTR
VILMCPQGKPFTQQVAESLAQEEHLVFICGHYEGYDERIREHLVTDELSIGDYVLTGGELAAITMTDAIVRLIPESIKEE
SHLDDSFSTGLLEYPQYTRPADYNGMKVPDVLLSGNHKHIESWRHEMRLKRTFERRPDLLEHYPLTQSDKEYLEHLKRDK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=42.3236514522822, Blast_Score=197, Evalue=4e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_MACCJ (B9EBB9)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560226.1
- GeneID:   7389510
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0823
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27775; Mature: 27775

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDYLTLFPEMFDVLNHSIMKRAQEKGIVELNTVNFRDYSGNKHNQVDDYPYGGGQGMV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEE
LKPEPIFNAMNAIDKTSKTRVILMCPQGKPFTQQVAESLAQEEHLVFICGHYEGYDERIR
ECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
EHLVTDELSIGDYVLTGGELAAITMTDAIVRLIPESIKEESHLDDSFSTGLLEYPQYTRP
HHHHHCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
ADYNGMKVPDVLLSGNHKHIESWRHEMRLKRTFERRPDLLEHYPLTQSDKEYLEHLKRDK
CCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKIDYLTLFPEMFDVLNHSIMKRAQEKGIVELNTVNFRDYSGNKHNQVDDYPYGGGQGMV
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCEE
LKPEPIFNAMNAIDKTSKTRVILMCPQGKPFTQQVAESLAQEEHLVFICGHYEGYDERIR
ECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
EHLVTDELSIGDYVLTGGELAAITMTDAIVRLIPESIKEESHLDDSFSTGLLEYPQYTRP
HHHHHCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCC
ADYNGMKVPDVLLSGNHKHIESWRHEMRLKRTFERRPDLLEHYPLTQSDKEYLEHLKRDK
CCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA