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The map label for this gene is phoB [H]

Identifier: 222151039

GI number: 222151039

Start: 854605

End: 856002

Strand: Direct

Name: phoB [H]

Synonym: MCCL_0790

Alternate gene names: 222151039

Gene position: 854605-856002 (Clockwise)

Preceding gene: 222151038

Following gene: 222151041

Centisome position: 40.65

GC content: 38.48

Gene sequence:

>1398_bases
ATGACTAGATTTCGTTATATCACTGCTGGTACATTAGCTGTATCTGCAATATTATCTGCAATTGCGCCGACCGCTGTATT
TGCGGGAGGAAAATCTTTGCCACAAAATAATGGTGTAAAATCAGAAATGGTTCAATGTGGTAATAAACAGAAACCAAAAA
ATGTTATCTTTATGATTGGTGACGGAATGGGACCTTCTTATACTTCAGCATATCGATATATGGAAGATAAGGATGAGACG
CATGAATTAGATCCAGTTGCTTTCGACCATTATTTATTAGGTGCACAACGCACAATTGCTGATGATGATCATGAAAACAT
CACTGATTCAGCAGCAGCAGCGACAGCGATGAGCGCAGGAACTAAAACTTATAATAATGCAATCGCTGTTGATAAACAGA
AGAAAGAAGTAAAGACTGTGCTTGAGCAGGCGAAGAAAGTAGGTAAAGCTACGGGTCTGGTTGCGACAAGTGAGATTACC
CATGCAACCCCTGCCGCATTTGGGGCACATGACATTTCTAGAAAGAATATGCCGCAAATTGCAGATGATTACTATGATGA
AATGATAAATGGAGAGCACAAGGTAGATATAATGCTTGGTGGTGGTAAAGAATACTTTGAACGTAAGGATAGAAATTTAG
TCAAAGCATTCCAAAAAGATGGCTATCACTATGTAACAGATAAAAAATCATTGGTAAAAGCAACACAATCAAATAACCAA
CAAATACTAGGACTGTTCTCACAAGGTGGGATGCCGAAGATGATTGATCGTGATGAAAATATGCCATCACTTAAGGATAT
GACAGAAACGGCATTGACTAAGTTAAAGAGTGATAAAGATGGATTCTTCCTGATGATTGAAGGTTCTCAGATTGACTGGG
CAGGACATGCAAATGATATTACTGGTGCGATGAGTGAAATGAGGGACTTTGATCTAGCTTTCCAGTCAGCAATTAACTTT
GCAAAGAAAGACTGTAACACACTAGTTATCGCAACTGCCGATCACTCGACAGGCGGGATGTCTATGGGACGAGGCGGAGA
ATACAACTTCCTGGTTGATCCTATCAAACAGATGAAGCATACACCTGAATATTTGAGAGCAGAGTTACTGAAAAAAGATG
CAAATATCGACAAAATAATTACAGAAAATATTGGATTTGCATTAGAAGCTACTGAAATTAAAGGTATTAAAGAAGCTATT
GCTAAAAGAGATGCTGGAAAGATTGAAGATGCAATTGAAGCGACAGTTAACAAACGTAGCTACACAGGATGGACAACAGA
TGGACATACAGGAGAAGATGTAAACGTCTATGGATATGGTCCAGGAGCACAAGCTTGGAGAGGTAATATGGATAATACAG
ATAACGCAAAACGAATTTTTGAATATCTTAAGAAATAA

Upstream 100 bases:

>100_bases
ATCCTTAGTGAATTATTTACAAAGGTATTATATTCTCTTAATAATTCATTGTTAACCTAAACAAGTAATAAAAATTTCTT
TTACACTTGGAGGAATATTA

Downstream 100 bases:

>100_bases
AAAAATGAATGTGCCGAACGGCACATTCATTTTTTTAAACCTTTAGATTAAGCTGTAACCCATTGAGATGGACCAGCTGT
ATTGTATAACTTAACAGCTG

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase III; APase III [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTRFRYITAGTLAVSAILSAIAPTAVFAGGKSLPQNNGVKSEMVQCGNKQKPKNVIFMIGDGMGPSYTSAYRYMEDKDET
HELDPVAFDHYLLGAQRTIADDDHENITDSAAAATAMSAGTKTYNNAIAVDKQKKEVKTVLEQAKKVGKATGLVATSEIT
HATPAAFGAHDISRKNMPQIADDYYDEMINGEHKVDIMLGGGKEYFERKDRNLVKAFQKDGYHYVTDKKSLVKATQSNNQ
QILGLFSQGGMPKMIDRDENMPSLKDMTETALTKLKSDKDGFFLMIEGSQIDWAGHANDITGAMSEMRDFDLAFQSAINF
AKKDCNTLVIATADHSTGGMSMGRGGEYNFLVDPIKQMKHTPEYLRAELLKKDANIDKIITENIGFALEATEIKGIKEAI
AKRDAGKIEDAIEATVNKRSYTGWTTDGHTGEDVNVYGYGPGAQAWRGNMDNTDNAKRIFEYLKK

Sequences:

>Translated_465_residues
MTRFRYITAGTLAVSAILSAIAPTAVFAGGKSLPQNNGVKSEMVQCGNKQKPKNVIFMIGDGMGPSYTSAYRYMEDKDET
HELDPVAFDHYLLGAQRTIADDDHENITDSAAAATAMSAGTKTYNNAIAVDKQKKEVKTVLEQAKKVGKATGLVATSEIT
HATPAAFGAHDISRKNMPQIADDYYDEMINGEHKVDIMLGGGKEYFERKDRNLVKAFQKDGYHYVTDKKSLVKATQSNNQ
QILGLFSQGGMPKMIDRDENMPSLKDMTETALTKLKSDKDGFFLMIEGSQIDWAGHANDITGAMSEMRDFDLAFQSAINF
AKKDCNTLVIATADHSTGGMSMGRGGEYNFLVDPIKQMKHTPEYLRAELLKKDANIDKIITENIGFALEATEIKGIKEAI
AKRDAGKIEDAIEATVNKRSYTGWTTDGHTGEDVNVYGYGPGAQAWRGNMDNTDNAKRIFEYLKK
>Mature_464_residues
TRFRYITAGTLAVSAILSAIAPTAVFAGGKSLPQNNGVKSEMVQCGNKQKPKNVIFMIGDGMGPSYTSAYRYMEDKDETH
ELDPVAFDHYLLGAQRTIADDDHENITDSAAAATAMSAGTKTYNNAIAVDKQKKEVKTVLEQAKKVGKATGLVATSEITH
ATPAAFGAHDISRKNMPQIADDYYDEMINGEHKVDIMLGGGKEYFERKDRNLVKAFQKDGYHYVTDKKSLVKATQSNNQQ
ILGLFSQGGMPKMIDRDENMPSLKDMTETALTKLKSDKDGFFLMIEGSQIDWAGHANDITGAMSEMRDFDLAFQSAINFA
KKDCNTLVIATADHSTGGMSMGRGGEYNFLVDPIKQMKHTPEYLRAELLKKDANIDKIITENIGFALEATEIKGIKEAIA
KRDAGKIEDAIEATVNKRSYTGWTTDGHTGEDVNVYGYGPGAQAWRGNMDNTDNAKRIFEYLKK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=451, Percent_Identity=28.6031042128603, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI157266296, Length=451, Percent_Identity=28.6031042128603, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI157266292, Length=445, Percent_Identity=27.8651685393258, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI116734717, Length=341, Percent_Identity=28.4457478005865, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI294660770, Length=332, Percent_Identity=27.710843373494, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI294660772, Length=275, Percent_Identity=28.3636363636364, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI48994877, Length=338, Percent_Identity=37.5739644970414, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320689, Length=476, Percent_Identity=33.8235294117647, Blast_Score=216, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24651554, Length=475, Percent_Identity=29.8947368421053, Blast_Score=142, Evalue=3e-34,
Organism=Drosophila melanogaster, GI18859923, Length=423, Percent_Identity=27.8959810874704, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355981, Length=338, Percent_Identity=29.585798816568, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21355149, Length=465, Percent_Identity=27.3118279569892, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24651556, Length=443, Percent_Identity=29.3453724604966, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21355151, Length=340, Percent_Identity=30, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24585213, Length=344, Percent_Identity=29.0697674418605, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24657842, Length=454, Percent_Identity=27.0925110132159, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24657835, Length=458, Percent_Identity=27.292576419214, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI85725200, Length=337, Percent_Identity=28.486646884273, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI85815833, Length=337, Percent_Identity=28.486646884273, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921912, Length=337, Percent_Identity=28.486646884273, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24657827, Length=461, Percent_Identity=27.3318872017354, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI21358067, Length=462, Percent_Identity=25.974025974026, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51134; Mature: 51003

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFRYITAGTLAVSAILSAIAPTAVFAGGKSLPQNNGVKSEMVQCGNKQKPKNVIFMIG
CCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEE
DGMGPSYTSAYRYMEDKDETHELDPVAFDHYLLGAQRTIADDDHENITDSAAAATAMSAG
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHCC
TKTYNNAIAVDKQKKEVKTVLEQAKKVGKATGLVATSEITHATPAAFGAHDISRKNMPQI
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHCCHHCCCCCCCHH
ADDYYDEMINGEHKVDIMLGGGKEYFERKDRNLVKAFQKDGYHYVTDKKSLVKATQSNNQ
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCC
QILGLFSQGGMPKMIDRDENMPSLKDMTETALTKLKSDKDGFFLMIEGSQIDWAGHANDI
EEEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHH
TGAMSEMRDFDLAFQSAINFAKKDCNTLVIATADHSTGGMSMGRGGEYNFLVDPIKQMKH
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
TPEYLRAELLKKDANIDKIITENIGFALEATEIKGIKEAIAKRDAGKIEDAIEATVNKRS
CHHHHHHHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
YTGWTTDGHTGEDVNVYGYGPGAQAWRGNMDNTDNAKRIFEYLKK
CCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHC
>Mature Secondary Structure 
TRFRYITAGTLAVSAILSAIAPTAVFAGGKSLPQNNGVKSEMVQCGNKQKPKNVIFMIG
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEE
DGMGPSYTSAYRYMEDKDETHELDPVAFDHYLLGAQRTIADDDHENITDSAAAATAMSAG
CCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHCC
TKTYNNAIAVDKQKKEVKTVLEQAKKVGKATGLVATSEITHATPAAFGAHDISRKNMPQI
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCHHCCHHCCCCCCCHH
ADDYYDEMINGEHKVDIMLGGGKEYFERKDRNLVKAFQKDGYHYVTDKKSLVKATQSNNQ
HHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCC
QILGLFSQGGMPKMIDRDENMPSLKDMTETALTKLKSDKDGFFLMIEGSQIDWAGHANDI
EEEEEECCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCHH
TGAMSEMRDFDLAFQSAINFAKKDCNTLVIATADHSTGGMSMGRGGEYNFLVDPIKQMKH
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
TPEYLRAELLKKDANIDKIITENIGFALEATEIKGIKEAIAKRDAGKIEDAIEATVNKRS
CHHHHHHHHHHHCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
YTGWTTDGHTGEDVNVYGYGPGAQAWRGNMDNTDNAKRIFEYLKK
CCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1898729; 9202461; 9384377; 2113910; 2105301 [H]