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The map label for this gene is mraW

Identifier: 222151001

GI number: 222151001

Start: 809423

End: 810355

Strand: Direct

Name: mraW

Synonym: MCCL_0751

Alternate gene names: 222151001

Gene position: 809423-810355 (Clockwise)

Preceding gene: 222151000

Following gene: 222151002

Centisome position: 38.5

GC content: 39.66

Gene sequence:

>933_bases
ATGTTCCATCATGTAACAGTATTATTAGAAGAAACGGTCGATCAGCTGAATATTGATCCAAATGGGATATACGTCGACTG
TACGTTAGGTGGAGCAGGACACAGTCTATATCTTGTGAAGCAGTTGCAGGAAGGGCACCTCATATCGATTGATCAGGATC
AGACTGCGATTGATAACGCGCACATCATACTAAAAGATTATCTGGATAAGGTAACGTTTGTTAAGGATAATTTTCGTAAT
CTTTCTGATATCCTTGCACATCTTGATATTCAGAAGGTTGACGGGATTTTATATGATCTAGGAGTATCAAGTCCACAGCT
GGATGTAGGAGAACGTGGTTTCAGCTATCATCAAGAAGCTAAACTTGATATGCGCATGGACCAGACACAGACACTATCAG
CTTATGAAGTGGTCAATACATGGCCATACGAAAAGCTTGTTTCTATATTTTTCCGTTATGGTGAAGAGAAATTTTCTAAA
CAAATTGCAAGAAAGATTGAAGCTGAACGTGAGTTAAAGCCAATCGAAACAACGACTGAACTAGTTGAAATTATTAAAGC
AGCAATTCCGGCACCCGCAAGAAGAACAGGAGGACATCCCGCTAAGCGTGTCTTCCAGGCGATAAGAATTGCTGTCAATG
ATGAATTAGGTGCCTTTGAATCATCGATAGAGCAAGCAATTGATAGTGTTAAGCCGGGTGGTAGAATTTCAGTGATAACA
TTCCATTCTTTAGAAGACCGTTTATGTAAACAGGTCTTTCAGGAATACAGTAAAGGACCAGATATCCCTCGTGGATTACC
GGTCGTTCCCCCTGAATACCAGCCGAAACTAAAGAAAATCACTAGAAAACCAATTACATCCTCAAATGAAGAGCTCGAAC
ATAATAATAGAGCGAGAAGTGCAAAGCTTAGAGTCGTAGAAATTTTAAAATAA

Upstream 100 bases:

>100_bases
TTGGGATCGCAAGTTATGGTCTGATTTCTACGAAGAGACAGAAGAAGAATTTGAAACAATTGCAGAAGAATTAATTGATT
TTGATTTTTAGGAGGATTCT

Downstream 100 bases:

>100_bases
AGAGGTGACAACATGGCAGTAGAGTATATCAATCCGAACTATTACAATCAGGTCAATGAACAAGTTAAAGTTAAATCTGT
TCCAAAAACACATGTTGTTT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MFHHVTVLLEETVDQLNIDPNGIYVDCTLGGAGHSLYLVKQLQEGHLISIDQDQTAIDNAHIILKDYLDKVTFVKDNFRN
LSDILAHLDIQKVDGILYDLGVSSPQLDVGERGFSYHQEAKLDMRMDQTQTLSAYEVVNTWPYEKLVSIFFRYGEEKFSK
QIARKIEAERELKPIETTTELVEIIKAAIPAPARRTGGHPAKRVFQAIRIAVNDELGAFESSIEQAIDSVKPGGRISVIT
FHSLEDRLCKQVFQEYSKGPDIPRGLPVVPPEYQPKLKKITRKPITSSNEELEHNNRARSAKLRVVEILK

Sequences:

>Translated_310_residues
MFHHVTVLLEETVDQLNIDPNGIYVDCTLGGAGHSLYLVKQLQEGHLISIDQDQTAIDNAHIILKDYLDKVTFVKDNFRN
LSDILAHLDIQKVDGILYDLGVSSPQLDVGERGFSYHQEAKLDMRMDQTQTLSAYEVVNTWPYEKLVSIFFRYGEEKFSK
QIARKIEAERELKPIETTTELVEIIKAAIPAPARRTGGHPAKRVFQAIRIAVNDELGAFESSIEQAIDSVKPGGRISVIT
FHSLEDRLCKQVFQEYSKGPDIPRGLPVVPPEYQPKLKKITRKPITSSNEELEHNNRARSAKLRVVEILK
>Mature_310_residues
MFHHVTVLLEETVDQLNIDPNGIYVDCTLGGAGHSLYLVKQLQEGHLISIDQDQTAIDNAHIILKDYLDKVTFVKDNFRN
LSDILAHLDIQKVDGILYDLGVSSPQLDVGERGFSYHQEAKLDMRMDQTQTLSAYEVVNTWPYEKLVSIFFRYGEEKFSK
QIARKIEAERELKPIETTTELVEIIKAAIPAPARRTGGHPAKRVFQAIRIAVNDELGAFESSIEQAIDSVKPGGRISVIT
FHSLEDRLCKQVFQEYSKGPDIPRGLPVVPPEYQPKLKKITRKPITSSNEELEHNNRARSAKLRVVEILK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=333, Percent_Identity=32.4324324324324, Blast_Score=158, Evalue=5e-39,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=34.7593582887701, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=42.9936305732484, Blast_Score=243, Evalue=2e-65,
Organism=Drosophila melanogaster, GI62472493, Length=334, Percent_Identity=32.3353293413174, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI28571637, Length=334, Percent_Identity=32.3353293413174, Blast_Score=147, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_MACCJ (B9EB47)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560154.1
- ProteinModelPortal:   B9EB47
- GeneID:   7389923
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0751
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35101; Mature: 35101

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: BINDING 51-51 BINDING 78-78 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHHVTVLLEETVDQLNIDPNGIYVDCTLGGAGHSLYLVKQLQEGHLISIDQDQTAIDNA
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCHHHCCH
HIILKDYLDKVTFVKDNFRNLSDILAHLDIQKVDGILYDLGVSSPQLDVGERGFSYHQEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCCCCCCCCCCCCCCCHHHHC
KLDMRMDQTQTLSAYEVVNTWPYEKLVSIFFRYGEEKFSKQIARKIEAERELKPIETTTE
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVEIIKAAIPAPARRTGGHPAKRVFQAIRIAVNDELGAFESSIEQAIDSVKPGGRISVIT
HHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEE
FHSLEDRLCKQVFQEYSKGPDIPRGLPVVPPEYQPKLKKITRKPITSSNEELEHNNRARS
EHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCHHHH
AKLRVVEILK
HHHHHHHHCC
>Mature Secondary Structure
MFHHVTVLLEETVDQLNIDPNGIYVDCTLGGAGHSLYLVKQLQEGHLISIDQDQTAIDNA
CCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECCCCHHHCCH
HIILKDYLDKVTFVKDNFRNLSDILAHLDIQKVDGILYDLGVSSPQLDVGERGFSYHQEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEECCCCCCCCCCCCCCCCHHHHC
KLDMRMDQTQTLSAYEVVNTWPYEKLVSIFFRYGEEKFSKQIARKIEAERELKPIETTTE
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVEIIKAAIPAPARRTGGHPAKRVFQAIRIAVNDELGAFESSIEQAIDSVKPGGRISVIT
HHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEE
FHSLEDRLCKQVFQEYSKGPDIPRGLPVVPPEYQPKLKKITRKPITSSNEELEHNNRARS
EHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCHHHH
AKLRVVEILK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA