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The map label for this gene is icaB [H]

Identifier: 222150931

GI number: 222150931

Start: 734024

End: 734836

Strand: Direct

Name: icaB [H]

Synonym: MCCL_0681

Alternate gene names: 222150931

Gene position: 734024-734836 (Clockwise)

Preceding gene: 222150930

Following gene: 222150932

Centisome position: 34.91

GC content: 46.62

Gene sequence:

>813_bases
ATGAGATGGATTAGTCTGTTGTTAATTGCTCTGCTATTGCTGAATGGACAGCCGGTAGAGGCAAAGAGCAGTGATGTGTC
TAAGCAGTGTCTGGCGCTCAACTATCATCGACTGATCAAAGATGATCTGTTTACGAATGTCATCAGTCTGCTCACTTCGA
ATAAAGAGCTGAACTTCTATTCTATTAAGGATAGTGAGTTTGAGGCACAGATGGACTGGCTGGTTGCACATGACGCGACT
TTTGTTACAGAAGAGACGTTGATTCAGGACAAGCAGCGCGGTAAGTTTCCGGAGCGCTGTGTATGGGTGAACTTCGACGA
TATGGATCAGTCAGCGTTGAAACTGGCACATCCGATTCTCAAGGAACGCGGCATTCCGGCGACAGGTTTCGTGATTACGG
GTCATGTAGGGGATGACTCGTTCCATAACCTTAAGTTGATCGATCTGAAGGGCCTTAAGGAGATGGAAGCATCCGGCATA
TGGCAGTTCAATTCACATACACATGACCTGCATAACTTGAAGCGTTCGACGTCAGATCTAGTGAAGCACTCTAGCAAGGC
CGGTGCGGATATCGACAGGAGCGTCAAGTATCTCGATCAGCACTTTAACAAGAAGGCCACTTCTCTTGCCTATCCATACG
GCCAGTATGACGATGCAGTCATCGATATTCTGGAGCACTCGGCTATCCGTCATGCCTATGTACTGAAAGAGACGCCGATC
CATGTTGATGATGATAATTACACGCTGCCACGTATCCTGGTGAGCAGAGATTCATTTGAGAAAGTCGTAAAAAACTGGAA
AGGTTTTAATTGA

Upstream 100 bases:

>100_bases
GAGCTGGTGCGTACGATGATAGGTCTTTTAGCGGCGGTTGGTGTCATCACCGTCTATCTGCTAAGTACATTCAGTCTGAC
GAAATGGAGGCAGCGCCGTT

Downstream 100 bases:

>100_bases
TATGAAAAAATATAGACTGGAACTGAACTATGTAAGGGTGATCCTGTGCGTGATGATCGTCGTTACGCACGTCCTGACGG
AGTATACGAACGCCCATAGT

Product: hypothetical protein

Products: NA

Alternate protein names: PNAG N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase; Biofilm polysaccharide intercellular adhesin deacetylase; Biofilm PIA deacetylase; Intercellular adhesion protein B [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRWISLLLIALLLLNGQPVEAKSSDVSKQCLALNYHRLIKDDLFTNVISLLTSNKELNFYSIKDSEFEAQMDWLVAHDAT
FVTEETLIQDKQRGKFPERCVWVNFDDMDQSALKLAHPILKERGIPATGFVITGHVGDDSFHNLKLIDLKGLKEMEASGI
WQFNSHTHDLHNLKRSTSDLVKHSSKAGADIDRSVKYLDQHFNKKATSLAYPYGQYDDAVIDILEHSAIRHAYVLKETPI
HVDDDNYTLPRILVSRDSFEKVVKNWKGFN

Sequences:

>Translated_270_residues
MRWISLLLIALLLLNGQPVEAKSSDVSKQCLALNYHRLIKDDLFTNVISLLTSNKELNFYSIKDSEFEAQMDWLVAHDAT
FVTEETLIQDKQRGKFPERCVWVNFDDMDQSALKLAHPILKERGIPATGFVITGHVGDDSFHNLKLIDLKGLKEMEASGI
WQFNSHTHDLHNLKRSTSDLVKHSSKAGADIDRSVKYLDQHFNKKATSLAYPYGQYDDAVIDILEHSAIRHAYVLKETPI
HVDDDNYTLPRILVSRDSFEKVVKNWKGFN
>Mature_270_residues
MRWISLLLIALLLLNGQPVEAKSSDVSKQCLALNYHRLIKDDLFTNVISLLTSNKELNFYSIKDSEFEAQMDWLVAHDAT
FVTEETLIQDKQRGKFPERCVWVNFDDMDQSALKLAHPILKERGIPATGFVITGHVGDDSFHNLKLIDLKGLKEMEASGI
WQFNSHTHDLHNLKRSTSDLVKHSSKAGADIDRSVKYLDQHFNKKATSLAYPYGQYDDAVIDILEHSAIRHAYVLKETPI
HVDDDNYTLPRILVSRDSFEKVVKNWKGFN

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. In fact, the IcaB deacetylase converts 15 to 20% of the GlcNAc residues of PNAG to glucosamine. N-deacetylation is cr

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Secreted, cell wall. Note=Attached to the cell surface [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1786322, Length=240, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 30896; Mature: 30896

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWISLLLIALLLLNGQPVEAKSSDVSKQCLALNYHRLIKDDLFTNVISLLTSNKELNFY
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
SIKDSEFEAQMDWLVAHDATFVTEETLIQDKQRGKFPERCVWVNFDDMDQSALKLAHPIL
EECCCCHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHH
KERGIPATGFVITGHVGDDSFHNLKLIDLKGLKEMEASGIWQFNSHTHDLHNLKRSTSDL
HHCCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHCCCEEECCCCHHHHHHHHHHHHH
VKHSSKAGADIDRSVKYLDQHFNKKATSLAYPYGQYDDAVIDILEHSAIRHAYVLKETPI
HHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCE
HVDDDNYTLPRILVSRDSFEKVVKNWKGFN
EECCCCCCHHHHEECCHHHHHHHHHCCCCC
>Mature Secondary Structure
MRWISLLLIALLLLNGQPVEAKSSDVSKQCLALNYHRLIKDDLFTNVISLLTSNKELNFY
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
SIKDSEFEAQMDWLVAHDATFVTEETLIQDKQRGKFPERCVWVNFDDMDQSALKLAHPIL
EECCCCHHHHHHHHEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHH
KERGIPATGFVITGHVGDDSFHNLKLIDLKGLKEMEASGIWQFNSHTHDLHNLKRSTSDL
HHCCCCCCCEEEEEECCCCCCCCEEEEECCCCHHHHHCCCEEECCCCHHHHHHHHHHHHH
VKHSSKAGADIDRSVKYLDQHFNKKATSLAYPYGQYDDAVIDILEHSAIRHAYVLKETPI
HHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCE
HVDDDNYTLPRILVSRDSFEKVVKNWKGFN
EECCCCCCHHHHEECCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA