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The map label for this gene is icaA [H]

Identifier: 222150929

GI number: 222150929

Start: 732518

End: 733762

Strand: Direct

Name: icaA [H]

Synonym: MCCL_0679

Alternate gene names: 222150929

Gene position: 732518-733762 (Clockwise)

Preceding gene: 222150928

Following gene: 222150930

Centisome position: 34.84

GC content: 43.86

Gene sequence:

>1245_bases
ATGAAAGGTTTAACCAACTTCTTTGGTGGGTTTCTAATCGTCTATCCTGTACTGATGTCCTTAGTATGGATTATCGGTGC
AGTGCTCTATTTCTTACTTATAGAGCGAAGATTGAAGCAGACGCCACCAGAGAGGGATGAGTATGAAGGGATTTCCTTTC
TTATTCCTTGTTACAACGAGGCGGATACTGTAGAGGAGACGTTGGTCAATGTCTTGAAGCTTTCTTATCCGAAGAAAGAG
ATTATCTTGATTAATGATGGGAGCAGCGATAATACAGCAGAGAAAATTAACGCGCTCAAAGATCATTATGACTTCAGATT
CATCGATTTACAACAGAACAGAGGTAAGGCGAATGCGCTGAATACAGCAGTGAAACATGCACAGTATGATTTCGTCATGG
GTATCGATGCAGATACGATTATTGACGATGCCGCGCCTTACTTCATGATAGACAACTTTAGACATGCGCCAAATCTTGGT
GCTGTGACTGGCAATCCGCGTATCAGAAACAAGTCGTCTATCCTAGGCAAGATCCAGACAGTTGAATATGCAAGTATCAT
CGGAAGTATCAAGCGTGCACAGACGTTGAATGGCTACGTCAATACGATTTCAGGTGTGTTCACGCTGTTCAGAAAATCAG
CCCTTGAAGCGGTAGGGTACTGGGATGATGATATGATCACTGAAGATATTGCAGTGTCCTGGAAATTCCACATGCATCAC
TATGAAATCCGCTATGAACCCCGTGCGTTATGCTGGATGCTCGTTCCTGAGACGTTCACCGGCATATGGAAACAGCGTTT
GAGATGGGCTCAAGGGGGTCATGAGGTTGTCTTGAGAGAGTTTAAATCGATGCTCAAACAACCGAACCTGCCGTTATGGT
TATTATACCTGGAACAGCTTATTTCAATCATCTGGGTCTACTCCGTATTATTCCTGCTGGCTTTTACTATCATCAACATC
GACGTTCTGGATTATTACTTCTACGCCTACAATGTCAATTTGCTGATCCTTGCTTCTATCATTCTGACGACGGTGAACGT
CATTCAGTTTACGGTGTCGCTTGTGATAGATAGCAGATATGAGCGCAAGAACATCGTCTATCTGTTCTTCTTATGTTGGT
ATCCGACCTTCTATTGGTTGATTAATGCGTGTGTTGCAGTGTTTGCCTTTCCTAAAGCGCTGCGCCGTAAGAAAGGAGAG
TTTGCGACATGGTCAAGTCCGGACAGAGGCAACATCCAACGGTAA

Upstream 100 bases:

>100_bases
GGATATTATGGGAAACTATGTTGTGTACTTTTGTTATAGATTTTAATTTAAATCTTATTAAAAAAAGTATGAGGCTATAT
TTATAAAAGGAAGTGAAGGT

Downstream 100 bases:

>100_bases
AGTCTACTTTGAACTTCTTCAATGAGTGTGTGCTCTTTATACTGTCAGGCACGTTATGGTTATATTGTCTGATATCGGTG
TTCATCCTCTTTGCTTTGTT

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKGLTNFFGGFLIVYPVLMSLVWIIGAVLYFLLIERRLKQTPPERDEYEGISFLIPCYNEADTVEETLVNVLKLSYPKKE
IILINDGSSDNTAEKINALKDHYDFRFIDLQQNRGKANALNTAVKHAQYDFVMGIDADTIIDDAAPYFMIDNFRHAPNLG
AVTGNPRIRNKSSILGKIQTVEYASIIGSIKRAQTLNGYVNTISGVFTLFRKSALEAVGYWDDDMITEDIAVSWKFHMHH
YEIRYEPRALCWMLVPETFTGIWKQRLRWAQGGHEVVLREFKSMLKQPNLPLWLLYLEQLISIIWVYSVLFLLAFTIINI
DVLDYYFYAYNVNLLILASIILTTVNVIQFTVSLVIDSRYERKNIVYLFFLCWYPTFYWLINACVAVFAFPKALRRKKGE
FATWSSPDRGNIQR

Sequences:

>Translated_414_residues
MKGLTNFFGGFLIVYPVLMSLVWIIGAVLYFLLIERRLKQTPPERDEYEGISFLIPCYNEADTVEETLVNVLKLSYPKKE
IILINDGSSDNTAEKINALKDHYDFRFIDLQQNRGKANALNTAVKHAQYDFVMGIDADTIIDDAAPYFMIDNFRHAPNLG
AVTGNPRIRNKSSILGKIQTVEYASIIGSIKRAQTLNGYVNTISGVFTLFRKSALEAVGYWDDDMITEDIAVSWKFHMHH
YEIRYEPRALCWMLVPETFTGIWKQRLRWAQGGHEVVLREFKSMLKQPNLPLWLLYLEQLISIIWVYSVLFLLAFTIINI
DVLDYYFYAYNVNLLILASIILTTVNVIQFTVSLVIDSRYERKNIVYLFFLCWYPTFYWLINACVAVFAFPKALRRKKGE
FATWSSPDRGNIQR
>Mature_414_residues
MKGLTNFFGGFLIVYPVLMSLVWIIGAVLYFLLIERRLKQTPPERDEYEGISFLIPCYNEADTVEETLVNVLKLSYPKKE
IILINDGSSDNTAEKINALKDHYDFRFIDLQQNRGKANALNTAVKHAQYDFVMGIDADTIIDDAAPYFMIDNFRHAPNLG
AVTGNPRIRNKSSILGKIQTVEYASIIGSIKRAQTLNGYVNTISGVFTLFRKSALEAVGYWDDDMITEDIAVSWKFHMHH
YEIRYEPRALCWMLVPETFTGIWKQRLRWAQGGHEVVLREFKSMLKQPNLPLWLLYLEQLISIIWVYSVLFLLAFTIINI
DVLDYYFYAYNVNLLILASIILTTVNVIQFTVSLVIDSRYERKNIVYLFFLCWYPTFYWLINACVAVFAFPKALRRKKGE
FATWSSPDRGNIQR

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=405, Percent_Identity=40.4938271604938, Blast_Score=335, Evalue=4e-93,
Organism=Escherichia coli, GI1786560, Length=231, Percent_Identity=29.4372294372294, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 48000; Mature: 48000

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLTNFFGGFLIVYPVLMSLVWIIGAVLYFLLIERRLKQTPPERDEYEGISFLIPCYNE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
ADTVEETLVNVLKLSYPKKEIILINDGSSDNTAEKINALKDHYDFRFIDLQQNRGKANAL
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHH
NTAVKHAQYDFVMGIDADTIIDDAAPYFMIDNFRHAPNLGAVTGNPRIRNKSSILGKIQT
HHHHHHHHCEEEEECCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VEYASIIGSIKRAQTLNGYVNTISGVFTLFRKSALEAVGYWDDDMITEDIAVSWKFHMHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEEEEE
YEIRYEPRALCWMLVPETFTGIWKQRLRWAQGGHEVVLREFKSMLKQPNLPLWLLYLEQL
EEEEECCHHEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
ISIIWVYSVLFLLAFTIINIDVLDYYFYAYNVNLLILASIILTTVNVIQFTVSLVIDSRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERKNIVYLFFLCWYPTFYWLINACVAVFAFPKALRRKKGEFATWSSPDRGNIQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKGLTNFFGGFLIVYPVLMSLVWIIGAVLYFLLIERRLKQTPPERDEYEGISFLIPCYNE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
ADTVEETLVNVLKLSYPKKEIILINDGSSDNTAEKINALKDHYDFRFIDLQQNRGKANAL
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCHHHH
NTAVKHAQYDFVMGIDADTIIDDAAPYFMIDNFRHAPNLGAVTGNPRIRNKSSILGKIQT
HHHHHHHHCEEEEECCHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VEYASIIGSIKRAQTLNGYVNTISGVFTLFRKSALEAVGYWDDDMITEDIAVSWKFHMHH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEEEEE
YEIRYEPRALCWMLVPETFTGIWKQRLRWAQGGHEVVLREFKSMLKQPNLPLWLLYLEQL
EEEEECCHHEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHH
ISIIWVYSVLFLLAFTIINIDVLDYYFYAYNVNLLILASIILTTVNVIQFTVSLVIDSRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERKNIVYLFFLCWYPTFYWLINACVAVFAFPKALRRKKGEFATWSSPDRGNIQR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]