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The map label for this gene is capO [H]

Identifier: 222150908

GI number: 222150908

Start: 708915

End: 710171

Strand: Direct

Name: capO [H]

Synonym: MCCL_0658

Alternate gene names: 222150908

Gene position: 708915-710171 (Clockwise)

Preceding gene: 222150905

Following gene: 222150909

Centisome position: 33.72

GC content: 39.3

Gene sequence:

>1257_bases
TTGAAACTTACAACGATAGGTTTAGGTTACATCGGATTACCGACATCAATTATGTTTGCGAAACATGGGGTTGATGTTGT
TGGGGTTGATATTAAGCAAGAAGCTGTAGATAAATTAAATAACGGGCAGATTCATATAGAAGAACCTGGGTTACAAGAAG
CGTTGGAGGAAGTGCTGGCCTCAGGTAAGTTCAAAGCTTCGACGATACCAGAGCAGGCAGATGCTTATATTATTGCGGTA
CCCACACCAAATAACAATGATGAACATAAATCATGTGATATCTCAATCGTGATGTCTGGTGTTGAAAGTATTATCCCTTT
ACTTAAGAGAGGGGATACAGTAATCGTTGAGTCGACGATTGCACCGCGTACCATGGATGACCATGTGAAGCCATATCTCG
AGTCTAAAGGCTTTATAATTGGTGAAGATATCTATCTTGTCCATTGCCCTGAGCGTGTATTACCGGGCCAGATCATGCAT
GAATTAGTTTATAACAATCGCATTATCGGTGGAATCACACCTGCTTGTGTAGAAGCGGGTAAGCGCGTCTACGGGACTTT
CGTTAAAGGTGAAATGATTGAGACGCAGGCGAAGACTGCTGAGATGTCCAAACTAATGGAGAACACATATCGTGATGTAA
ATATCGCATTAGCGAATGAACTCGCTAAAATCTGCAACGATCTAGAGATTAATGTTAATGATGTGATTGAGATGGCAAAT
AAACATCCTCGAGTTAATTTACATACGCCAGGTCCTGGTGTCGGTGGTCACTGTTTAGCGGTCGATCCATATTTCATTAT
CGCAGAGGCCCATGAGCATGCATTATTGATCAAACTTGCACGTGATATCAATGTGTCTATGCCAGAATATGTTGTACATC
ATACGAAGGATATCTTATCTAGAATCGGCGGCAACAAAGTCACTGTATTTGGTCTGACATATAAAGGTGATGTTGATGAT
ATTCGTGAATCACCTGCATTTGACATTTATGAAATGTTAACCGCATCTCAATATGAAGTTGTCGCATATGATCCTCATGT
GAAGCAGGAGTTTGTTGAAGAAGATATGGGAACAGCGGTTAAAGACAGCGATTTAGTATTGATATTGAGTGATCATTCCG
AATTTAAGCAGCTCACTGATGGTGATTTTGATGGAATGAGAAACAGATATATTCTTGATACTAAGAACGTTGTTAAGACT
AAGGATGAATTTAAAGAAGTTAAATACTATAACTTCGGGAATTTATATAATTTATAA

Upstream 100 bases:

>100_bases
CCTCACTTTTTCATATTTTCTTAAGCTATACTACCCATGAAGTAGATAAAAATTATTACTTAATTAAATATGAGTTTTCT
TGAGAAAAGGATGGATTAAT

Downstream 100 bases:

>100_bases
CTACAACAACAGAATACGTTCGTATTCTGTTTGTTTTTTAATAAGGAGTTTCTATGAAAAGCATATTGCTCATCTCACAA
AATTTCTATCCTGAAATCGG

Product: capsular polysaccharide synthesis enzyme cap5O

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKLTTIGLGYIGLPTSIMFAKHGVDVVGVDIKQEAVDKLNNGQIHIEEPGLQEALEEVLASGKFKASTIPEQADAYIIAV
PTPNNNDEHKSCDISIVMSGVESIIPLLKRGDTVIVESTIAPRTMDDHVKPYLESKGFIIGEDIYLVHCPERVLPGQIMH
ELVYNNRIIGGITPACVEAGKRVYGTFVKGEMIETQAKTAEMSKLMENTYRDVNIALANELAKICNDLEINVNDVIEMAN
KHPRVNLHTPGPGVGGHCLAVDPYFIIAEAHEHALLIKLARDINVSMPEYVVHHTKDILSRIGGNKVTVFGLTYKGDVDD
IRESPAFDIYEMLTASQYEVVAYDPHVKQEFVEEDMGTAVKDSDLVLILSDHSEFKQLTDGDFDGMRNRYILDTKNVVKT
KDEFKEVKYYNFGNLYNL

Sequences:

>Translated_418_residues
MKLTTIGLGYIGLPTSIMFAKHGVDVVGVDIKQEAVDKLNNGQIHIEEPGLQEALEEVLASGKFKASTIPEQADAYIIAV
PTPNNNDEHKSCDISIVMSGVESIIPLLKRGDTVIVESTIAPRTMDDHVKPYLESKGFIIGEDIYLVHCPERVLPGQIMH
ELVYNNRIIGGITPACVEAGKRVYGTFVKGEMIETQAKTAEMSKLMENTYRDVNIALANELAKICNDLEINVNDVIEMAN
KHPRVNLHTPGPGVGGHCLAVDPYFIIAEAHEHALLIKLARDINVSMPEYVVHHTKDILSRIGGNKVTVFGLTYKGDVDD
IRESPAFDIYEMLTASQYEVVAYDPHVKQEFVEEDMGTAVKDSDLVLILSDHSEFKQLTDGDFDGMRNRYILDTKNVVKT
KDEFKEVKYYNFGNLYNL
>Mature_418_residues
MKLTTIGLGYIGLPTSIMFAKHGVDVVGVDIKQEAVDKLNNGQIHIEEPGLQEALEEVLASGKFKASTIPEQADAYIIAV
PTPNNNDEHKSCDISIVMSGVESIIPLLKRGDTVIVESTIAPRTMDDHVKPYLESKGFIIGEDIYLVHCPERVLPGQIMH
ELVYNNRIIGGITPACVEAGKRVYGTFVKGEMIETQAKTAEMSKLMENTYRDVNIALANELAKICNDLEINVNDVIEMAN
KHPRVNLHTPGPGVGGHCLAVDPYFIIAEAHEHALLIKLARDINVSMPEYVVHHTKDILSRIGGNKVTVFGLTYKGDVDD
IRESPAFDIYEMLTASQYEVVAYDPHVKQEFVEEDMGTAVKDSDLVLILSDHSEFKQLTDGDFDGMRNRYILDTKNVVKT
KDEFKEVKYYNFGNLYNL

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=408, Percent_Identity=24.2647058823529, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI48994968, Length=422, Percent_Identity=42.1800947867299, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1788340, Length=404, Percent_Identity=26.2376237623762, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560350, Length=454, Percent_Identity=23.7885462555066, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17136908, Length=447, Percent_Identity=22.8187919463087, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46493; Mature: 46493

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTTIGLGYIGLPTSIMFAKHGVDVVGVDIKQEAVDKLNNGQIHIEEPGLQEALEEVLA
CEEEEEECCEECCCHHHHHHHCCCEEEEECCHHHHHHHCCCCEEEEECCCHHHHHHHHHH
SGKFKASTIPEQADAYIIAVPTPNNNDEHKSCDISIVMSGVESIIPLLKRGDTVIVESTI
CCCEECCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
APRTMDDHVKPYLESKGFIIGEDIYLVHCPERVLPGQIMHELVYNNRIIGGITPACVEAG
CCCCCHHHHHHHHHCCCEEEECCEEEEECCCHHCCHHHHHHHHHCCEEECCCCHHHHHCC
KRVYGTFVKGEMIETQAKTAEMSKLMENTYRDVNIALANELAKICNDLEINVNDVIEMAN
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCHHHHHHHHC
KHPRVNLHTPGPGVGGHCLAVDPYFIIAEAHEHALLIKLARDINVSMPEYVVHHTKDILS
CCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEEEEEEHHHCCCCCHHHHHHHHHHHHH
RIGGNKVTVFGLTYKGDVDDIRESPAFDIYEMLTASQYEVVAYDPHVKQEFVEEDMGTAV
HCCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCC
KDSDLVLILSDHSEFKQLTDGDFDGMRNRYILDTKNVVKTKDEFKEVKYYNFGNLYNL
CCCCEEEEECCCHHHHHHCCCCCCCCCCCEEEECCHHHHCHHHHHHCCEECCCCCCCC
>Mature Secondary Structure
MKLTTIGLGYIGLPTSIMFAKHGVDVVGVDIKQEAVDKLNNGQIHIEEPGLQEALEEVLA
CEEEEEECCEECCCHHHHHHHCCCEEEEECCHHHHHHHCCCCEEEEECCCHHHHHHHHHH
SGKFKASTIPEQADAYIIAVPTPNNNDEHKSCDISIVMSGVESIIPLLKRGDTVIVESTI
CCCEECCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
APRTMDDHVKPYLESKGFIIGEDIYLVHCPERVLPGQIMHELVYNNRIIGGITPACVEAG
CCCCCHHHHHHHHHCCCEEEECCEEEEECCCHHCCHHHHHHHHHCCEEECCCCHHHHHCC
KRVYGTFVKGEMIETQAKTAEMSKLMENTYRDVNIALANELAKICNDLEINVNDVIEMAN
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHCCCCCCHHHHHHHHC
KHPRVNLHTPGPGVGGHCLAVDPYFIIAEAHEHALLIKLARDINVSMPEYVVHHTKDILS
CCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEEEEEEHHHCCCCCHHHHHHHHHHHHH
RIGGNKVTVFGLTYKGDVDDIRESPAFDIYEMLTASQYEVVAYDPHVKQEFVEEDMGTAV
HCCCCEEEEEEEEECCCHHHHHCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCC
KDSDLVLILSDHSEFKQLTDGDFDGMRNRYILDTKNVVKTKDEFKEVKYYNFGNLYNL
CCCCEEEEECCCHHHHHHCCCCCCCCCCCEEEECCHHHHCHHHHHHCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA