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The map label for this gene is ppnK [H]

Identifier: 222150871

GI number: 222150871

Start: 663948

End: 664748

Strand: Direct

Name: ppnK [H]

Synonym: MCCL_0621

Alternate gene names: 222150871

Gene position: 663948-664748 (Clockwise)

Preceding gene: 222150870

Following gene: 222150872

Centisome position: 31.58

GC content: 43.2

Gene sequence:

>801_bases
GTGATGAGATATGCAATATTGACTAAAGGTGACTCTAAAAGTAATGCTTTAAAGCAGAAGATGGTCAGTTATATGAATGA
CTTCAATATGACAGAAGATACAGCGACTCCTGAAATTGTCATCTCTGTTGGGGGAGATGGTACATTACTGCAGGCATTTC
ATACATATAGTCATCGACTGGAAGAAACGAGTTTTGTAGGTATACATACAGGACATCTTGGCTTCTATGCAGACTGGCTC
CCGCATGAAGTGGAAAAACTGATTATTGCAATCAACAATGATTCATTTCAGGTGATTGAATACCCGCTCGTGGAAGTTGT
CGTCAGATACGATGAAGGTAAGGCAAGTCGATATCTCGCGCTGAACGAAGCCACTGTAAAGACGCATAATGGTGCAACGC
TGGTCGCAGATATAAGTTTAAGAGGCGAGCTATTCGAACGCTTCAGAGGAGATGGCTTGTGTATTTCAACACCGAGTGGC
TCTACAGCATATAATAAAGCACTTGGCGGTGCACTGATACATCCTTCATTGGATGCGATTCAAGTGACCGAAATTGCTTC
GATTAACAATCGCGTGTTCCGTACGGTCGGCTCTCCGCTTGTGCTGCCTAAACATCATAATTGTCAGGTGGAACCTGCAA
ATCCAATCACGTTACAGCTTACATTTGATCATATCACTGCGGATCATAAAGGTGTTTCGTCGATACAGTACCGCGTTGCA
GATGAGAAAGTGAGATTTGCACGTTTCAGACCTTTTCCTTTCTGGAAACGTGTGCACGATTCATTTATCGCAAGCGAATG
A

Upstream 100 bases:

>100_bases
CATTTCTGCTGGATGAAGAGATGTCTGAAATTCGTGAAGAAATACAGGAAGCACAAAAATATTATAGCGAGCAACGCAGC
GAAAAACGCTAAGAGGTGAA

Downstream 100 bases:

>100_bases
AAGAGCTTACTTATGCAGTGACAGAACAGATGACAGTTAAGGCATTTCTTCAGAATCATCAATATAGCAGAAAGAGTATA
AGCGCCATTAAACAAAATGG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MMRYAILTKGDSKSNALKQKMVSYMNDFNMTEDTATPEIVISVGGDGTLLQAFHTYSHRLEETSFVGIHTGHLGFYADWL
PHEVEKLIIAINNDSFQVIEYPLVEVVVRYDEGKASRYLALNEATVKTHNGATLVADISLRGELFERFRGDGLCISTPSG
STAYNKALGGALIHPSLDAIQVTEIASINNRVFRTVGSPLVLPKHHNCQVEPANPITLQLTFDHITADHKGVSSIQYRVA
DEKVRFARFRPFPFWKRVHDSFIASE

Sequences:

>Translated_266_residues
MMRYAILTKGDSKSNALKQKMVSYMNDFNMTEDTATPEIVISVGGDGTLLQAFHTYSHRLEETSFVGIHTGHLGFYADWL
PHEVEKLIIAINNDSFQVIEYPLVEVVVRYDEGKASRYLALNEATVKTHNGATLVADISLRGELFERFRGDGLCISTPSG
STAYNKALGGALIHPSLDAIQVTEIASINNRVFRTVGSPLVLPKHHNCQVEPANPITLQLTFDHITADHKGVSSIQYRVA
DEKVRFARFRPFPFWKRVHDSFIASE
>Mature_266_residues
MMRYAILTKGDSKSNALKQKMVSYMNDFNMTEDTATPEIVISVGGDGTLLQAFHTYSHRLEETSFVGIHTGHLGFYADWL
PHEVEKLIIAINNDSFQVIEYPLVEVVVRYDEGKASRYLALNEATVKTHNGATLVADISLRGELFERFRGDGLCISTPSG
STAYNKALGGALIHPSLDAIQVTEIASINNRVFRTVGSPLVLPKHHNCQVEPANPITLQLTFDHITADHKGVSSIQYRVA
DEKVRFARFRPFPFWKRVHDSFIASE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=144, Percent_Identity=31.9444444444444, Blast_Score=68, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320794, Length=214, Percent_Identity=28.0373831775701, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325068, Length=207, Percent_Identity=23.6714975845411, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 29709; Mature: 29709

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRYAILTKGDSKSNALKQKMVSYMNDFNMTEDTATPEIVISVGGDGTLLQAFHTYSHRL
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
EETSFVGIHTGHLGFYADWLPHEVEKLIIAINNDSFQVIEYPLVEVVVRYDEGKASRYLA
CCCEEEEEEECCCCEEECCCHHCCEEEEEEECCCCEEEEEECEEEEEEEECCCCCCEEEE
LNEATVKTHNGATLVADISLRGELFERFRGDGLCISTPSGSTAYNKALGGALIHPSLDAI
EECEEEEECCCEEEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCCCEE
QVTEIASINNRVFRTVGSPLVLPKHHNCQVEPANPITLQLTFDHITADHKGVSSIQYRVA
EEEHHHHHCCHHHHHCCCCEEECCCCCCEECCCCCEEEEEEEEEECCCCCCCCCEEEEEC
DEKVRFARFRPFPFWKRVHDSFIASE
CCCEEEEECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MMRYAILTKGDSKSNALKQKMVSYMNDFNMTEDTATPEIVISVGGDGTLLQAFHTYSHRL
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHH
EETSFVGIHTGHLGFYADWLPHEVEKLIIAINNDSFQVIEYPLVEVVVRYDEGKASRYLA
CCCEEEEEEECCCCEEECCCHHCCEEEEEEECCCCEEEEEECEEEEEEEECCCCCCEEEE
LNEATVKTHNGATLVADISLRGELFERFRGDGLCISTPSGSTAYNKALGGALIHPSLDAI
EECEEEEECCCEEEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCCCEE
QVTEIASINNRVFRTVGSPLVLPKHHNCQVEPANPITLQLTFDHITADHKGVSSIQYRVA
EEEHHHHHCCHHHHHCCCCEEECCCCCCEECCCCCEEEEEEEEEECCCCCCCCCEEEEEC
DEKVRFARFRPFPFWKRVHDSFIASE
CCCEEEEECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA