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The map label for this gene is malS [C]

Identifier: 222150846

GI number: 222150846

Start: 636819

End: 638219

Strand: Direct

Name: malS [C]

Synonym: MCCL_0596

Alternate gene names: 222150846

Gene position: 636819-638219 (Clockwise)

Preceding gene: 222150845

Following gene: 222150849

Centisome position: 30.29

GC content: 37.26

Gene sequence:

>1401_bases
ATGAAAAGAATTTCAATCATATTGATGATATTACTGCTATCATTCAGTGCGCTACCTGCACAAGCAGCCAGTCAGGACGA
AATGCGTATCTATAGCATTCTTACTGATCGATTTATGAATGGTGATGAGAATAATAATAAAGATATTCATAATGACAAAG
ACAATAATCTGCCTTACGGTGGGGACTTTAAAGGCATCATCAATAAAGTAGACTATATTAAGAAGATGGGCTTTAATGCA
ATTCATGTCTCACCGGTGTTTGACCATGAGAAGAATGATTATCTAGGGTATAAGATTAATAATTATAATCAGATTTCTAA
AACATTCGGTGGAGAGAAGGACTTTAAACAGCTCGTATATCTTGCACATAAAAATAATATGAAAGTCATCGTCGATATGC
CTGTTGTTGCAACGGATGATTTCACAGCAACAGAAGACACTAAGATGAACGCAATAGAGAAGTCGTATTATAAAGATACA
CCGATGATTGATCTAACGAATGAAGCAAATATTAAGCGCTATAAGCAGCTCATGACAGAATTCGTAAACAAAACGAAAGT
CGACGGCTTATCGATGTATGTACTTCAGGATAATGTCGATCTTTCAAAAGTATTTCCTGAGAATATAACAAAGATTGCTA
TTACCAATAGCGATGTTAAAGTAACCGGCTATGATTACATTCAGACTGGGAAGACATCAGAGCAAATTGCACAAGCATTC
AAGAATGTTGATGAGGAAATCCCAGAGCAGTACAATAAGAAGGAGCTGCTTTCAGCTGATAACTTCTTTACCCCACGTTT
CACAAGCTATGCGGTTCATGAAAATATGTTCCCGGGAACACGTATTAAACAGATGATGGGCTATCTGTTTGTACAAAAGC
AACCAGTCAGTATGACTTATGGTACTGAGATTGCGATGAATGGCGAAAAACTTCCTGATACACATCAACTACTCGATTTT
AGAACGGATAAAGAAGTGGTGGAATACTTAGAACAGACGAGCGAAGTTTATCATAAGTACAAAGAAATGTTCGATGGTAC
ATCTAAAGTGATCTATAACGAAAAAGGTGAGCAGCTTATCTATTTCGATACAGACAAGGTAGACTTCATCTATAATATTA
ACGATACAAGCAAGTCTACGAACGTTAAGCTGACGGACAAAGACATACCAGGCGATAAGATGCTCAGCGGATTGCTTATT
GGAGATAGAATCCATGTGAAGGACGGCACATTTAATTTGATAACGAACCGTGAAGAAACGGAGCTTTACGCACTCGTTCC
ACCACGTGGACTGAACTTAGGTTATGTGATCGCATCACTGTTAACAATCGTGCTCTTTACAGTATTTATCATCGGTGCAG
CAAGAAATAGGAAGAAATATGTCAATAATTTAAGAAAATAG

Upstream 100 bases:

>100_bases
GTTTCTCGCCGTCTTTTTTATTTGAATATTTTATGAACTCTTACATAAACGATAAGTAATTTAGTATAATCAATGTAATA
ACTTAAGTGAGGGTATTCAA

Downstream 100 bases:

>100_bases
AAATAACGAAAAGACAATCGGCGTATGCGGATTGTCTTTTTTGTTGGGAGATGGGGTGCATAAACAAAGTGCAGCACCAT
ATAAGATGCCCAAATATTGG

Product: hypothetical protein

Products: NA

Alternate protein names: Beta-amylase; Alpha-amylase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKRISIILMILLLSFSALPAQAASQDEMRIYSILTDRFMNGDENNNKDIHNDKDNNLPYGGDFKGIINKVDYIKKMGFNA
IHVSPVFDHEKNDYLGYKINNYNQISKTFGGEKDFKQLVYLAHKNNMKVIVDMPVVATDDFTATEDTKMNAIEKSYYKDT
PMIDLTNEANIKRYKQLMTEFVNKTKVDGLSMYVLQDNVDLSKVFPENITKIAITNSDVKVTGYDYIQTGKTSEQIAQAF
KNVDEEIPEQYNKKELLSADNFFTPRFTSYAVHENMFPGTRIKQMMGYLFVQKQPVSMTYGTEIAMNGEKLPDTHQLLDF
RTDKEVVEYLEQTSEVYHKYKEMFDGTSKVIYNEKGEQLIYFDTDKVDFIYNINDTSKSTNVKLTDKDIPGDKMLSGLLI
GDRIHVKDGTFNLITNREETELYALVPPRGLNLGYVIASLLTIVLFTVFIIGAARNRKKYVNNLRK

Sequences:

>Translated_466_residues
MKRISIILMILLLSFSALPAQAASQDEMRIYSILTDRFMNGDENNNKDIHNDKDNNLPYGGDFKGIINKVDYIKKMGFNA
IHVSPVFDHEKNDYLGYKINNYNQISKTFGGEKDFKQLVYLAHKNNMKVIVDMPVVATDDFTATEDTKMNAIEKSYYKDT
PMIDLTNEANIKRYKQLMTEFVNKTKVDGLSMYVLQDNVDLSKVFPENITKIAITNSDVKVTGYDYIQTGKTSEQIAQAF
KNVDEEIPEQYNKKELLSADNFFTPRFTSYAVHENMFPGTRIKQMMGYLFVQKQPVSMTYGTEIAMNGEKLPDTHQLLDF
RTDKEVVEYLEQTSEVYHKYKEMFDGTSKVIYNEKGEQLIYFDTDKVDFIYNINDTSKSTNVKLTDKDIPGDKMLSGLLI
GDRIHVKDGTFNLITNREETELYALVPPRGLNLGYVIASLLTIVLFTVFIIGAARNRKKYVNNLRK
>Mature_466_residues
MKRISIILMILLLSFSALPAQAASQDEMRIYSILTDRFMNGDENNNKDIHNDKDNNLPYGGDFKGIINKVDYIKKMGFNA
IHVSPVFDHEKNDYLGYKINNYNQISKTFGGEKDFKQLVYLAHKNNMKVIVDMPVVATDDFTATEDTKMNAIEKSYYKDT
PMIDLTNEANIKRYKQLMTEFVNKTKVDGLSMYVLQDNVDLSKVFPENITKIAITNSDVKVTGYDYIQTGKTSEQIAQAF
KNVDEEIPEQYNKKELLSADNFFTPRFTSYAVHENMFPGTRIKQMMGYLFVQKQPVSMTYGTEIAMNGEKLPDTHQLLDF
RTDKEVVEYLEQTSEVYHKYKEMFDGTSKVIYNEKGEQLIYFDTDKVDFIYNINDTSKSTNVKLTDKDIPGDKMLSGLLI
GDRIHVKDGTFNLITNREETELYALVPPRGLNLGYVIASLLTIVLFTVFIIGAARNRKKYVNNLRK

Specific function: The precursor protein is proteolytically cleaved to produce multiform beta-amylases and a 48 kDa alpha-amylase after secretion [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=121, Percent_Identity=31.404958677686, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322245, Length=113, Percent_Identity=33.6283185840708, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321726, Length=81, Percent_Identity=41.9753086419753, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324416, Length=81, Percent_Identity=41.9753086419753, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=81, Percent_Identity=41.9753086419753, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=81, Percent_Identity=41.9753086419753, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=113, Percent_Identity=34.5132743362832, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319776, Length=113, Percent_Identity=34.5132743362832, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR005085
- InterPro:   IPR008985
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR001554
- InterPro:   IPR018238
- InterPro:   IPR000125
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF03423 CBM_25; PF01373 Glyco_hydro_14 [H]

EC number: =3.2.1.2; =3.2.1.1 [H]

Molecular weight: Translated: 53569; Mature: 53569

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRISIILMILLLSFSALPAQAASQDEMRIYSILTDRFMNGDENNNKDIHNDKDNNLPYG
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GDFKGIINKVDYIKKMGFNAIHVSPVFDHEKNDYLGYKINNYNQISKTFGGEKDFKQLVY
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECEEECCCHHHHHHCCCHHHHHHHHH
LAHKNNMKVIVDMPVVATDDFTATEDTKMNAIEKSYYKDTPMIDLTNEANIKRYKQLMTE
HEECCCCEEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHH
FVNKTKVDGLSMYVLQDNVDLSKVFPENITKIAITNSDVKVTGYDYIQTGKTSEQIAQAF
HHHHCCCCCEEEEEEECCCCHHHHCCCCCEEEEEECCCEEEECCHHEECCCCHHHHHHHH
KNVDEEIPEQYNKKELLSADNFFTPRFTSYAVHENMFPGTRIKQMMGYLFVQKQPVSMTY
HHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHEECCCCEEEE
GTEIAMNGEKLPDTHQLLDFRTDKEVVEYLEQTSEVYHKYKEMFDGTSKVIYNEKGEQLI
CCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEE
YFDTDKVDFIYNINDTSKSTNVKLTDKDIPGDKMLSGLLIGDRIHVKDGTFNLITNREET
EEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHEEECCEEEECCCCEEEEECCCCC
ELYALVPPRGLNLGYVIASLLTIVLFTVFIIGAARNRKKYVNNLRK
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MKRISIILMILLLSFSALPAQAASQDEMRIYSILTDRFMNGDENNNKDIHNDKDNNLPYG
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GDFKGIINKVDYIKKMGFNAIHVSPVFDHEKNDYLGYKINNYNQISKTFGGEKDFKQLVY
CCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECEEECCCHHHHHHCCCHHHHHHHHH
LAHKNNMKVIVDMPVVATDDFTATEDTKMNAIEKSYYKDTPMIDLTNEANIKRYKQLMTE
HEECCCCEEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHH
FVNKTKVDGLSMYVLQDNVDLSKVFPENITKIAITNSDVKVTGYDYIQTGKTSEQIAQAF
HHHHCCCCCEEEEEEECCCCHHHHCCCCCEEEEEECCCEEEECCHHEECCCCHHHHHHHH
KNVDEEIPEQYNKKELLSADNFFTPRFTSYAVHENMFPGTRIKQMMGYLFVQKQPVSMTY
HHHHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHEECCCCEEEE
GTEIAMNGEKLPDTHQLLDFRTDKEVVEYLEQTSEVYHKYKEMFDGTSKVIYNEKGEQLI
CCEEEECCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEE
YFDTDKVDFIYNINDTSKSTNVKLTDKDIPGDKMLSGLLIGDRIHVKDGTFNLITNREET
EEECCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHEEECCEEEECCCCEEEEECCCCC
ELYALVPPRGLNLGYVIASLLTIVLFTVFIIGAARNRKKYVNNLRK
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2435707; 2464578; 2438660; 1827035 [H]