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The map label for this gene is eno

Identifier: 222150775

GI number: 222150775

Start: 558057

End: 559361

Strand: Direct

Name: eno

Synonym: MCCL_0525

Alternate gene names: 222150775

Gene position: 558057-559361 (Clockwise)

Preceding gene: 222150774

Following gene: 222150776

Centisome position: 26.54

GC content: 37.93

Gene sequence:

>1305_bases
ATGCCAATTATTACAGATGTATATGCACGTGAAGTATTAGATTCAAGAGGTAATCCAACAATTGAGGTAGAAGTATTCAC
TGAAAGTGGTGCACTAGGGCGTGCGTTAGTACCTTCAGGTGCTTCAACTGGTGAACATGAAGCAGTTGAATTACGCGATG
GCGATAAAGATCGCTACATGGGTAAAGGTGTTTTAAAAGCTGTAGAAAACGTTAATGAAATTATCGCACCTGAGATTATT
GAAGGTGACTTCTCAGTACTTGATCAAGTATCAATCGATAAAATGATGATCGCTTTAGATGGTACAGATAATAAAGGTAA
ATTAGGTGCCAATGCAATTTTAGGTGTATCAATCGCTGTAGCACGTGCTGCTGCTGACTATTTAGGTGTACCTTTGTACA
AATATCTAGGTGGATTTAACGGTACTGAATTACCAGTTCCGATGATGAACATCGTTAACGGTGGTTCTCACTCTGATGCA
CCAATCGCATTCCAGGAGTTCATGGTATTACCAGTAGGAGCACCAAACTTCAAAGAAGCATTACGCTGGGGCGCTGAAAT
TTTCCACAACTTAGCGAAAATCTTAAAAGGCCGTAATTTATCAACTGCTGTAGGTGATGAAGGTGGATTCGCACCGACAT
TTGAAGGTACTGAAGATGCTGTTGAAACGATTTTAGAAGCGATTAAAGCTGCAGGACTTGAGCCAGGCAAAGATGTATTC
TTAGGATTTGACTGTGCTGCTTCTGAGTTCTTCGAAAATGGTGTGTATGACTACGCTAAATTTGAAGGTGAAAACGGTAA
AAAACGTACTTCTTCTGAACAAGTAGATTATTTAGAAGAATTAGTAGACAAATATCCAATCATCACAATTGAAGATGGTA
TGGATGAAAATGACTGGGAAGGCTGGAAAGCATTAACAGATCGTTTAGGCAATAAGGTACAACTTGTTGGTGATGATTTA
TTCGTTACGAACACTAAGATTTTAGAACGCGGTATTAACGAAGGCGTTGGTAACTCAATCTTAATTAAAGTGAACCAAAT
TGGTACTTTAACTGAAACGTTCGAAGCAATTGAAATGGCACAAAAAGCAGGTTACACTGCAGTTGTTTCACACCGTTCTG
GTGAAACTGAAGATACTACAATTGCGGACATCGCAGTTGCAACAAACGCTGGACAAATTAAAACAGGTTCATTATCACGT
ACTGACCGTATTGCGAAATACAATCAATTATTACGTATTGAAGATGAATTATTTGAAACTGCAAAATTTAAAGGTTTAAA
TGCATTCTACAATTTAAATAAATAA

Upstream 100 bases:

>100_bases
AAAGGTTAATGTTATACAGAAAATGAATTACAAATAATTGATTATTTCAATTAAATCAATAATAATAAGTAAGAACACAA
ATTGAGAGGAGTTTTTATAA

Downstream 100 bases:

>100_bases
TTGCATTTATAAAGGAGAGACGTGACGACGTCTCTTCCTTTTTTTATGTATATATGGTAAAATGTTAAGAGTTATCATAT
AGGAGGACCACTCATGAATA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPIITDVYAREVLDSRGNPTIEVEVFTESGALGRALVPSGASTGEHEAVELRDGDKDRYMGKGVLKAVENVNEIIAPEII
EGDFSVLDQVSIDKMMIALDGTDNKGKLGANAILGVSIAVARAAADYLGVPLYKYLGGFNGTELPVPMMNIVNGGSHSDA
PIAFQEFMVLPVGAPNFKEALRWGAEIFHNLAKILKGRNLSTAVGDEGGFAPTFEGTEDAVETILEAIKAAGLEPGKDVF
LGFDCAASEFFENGVYDYAKFEGENGKKRTSSEQVDYLEELVDKYPIITIEDGMDENDWEGWKALTDRLGNKVQLVGDDL
FVTNTKILERGINEGVGNSILIKVNQIGTLTETFEAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSR
TDRIAKYNQLLRIEDELFETAKFKGLNAFYNLNK

Sequences:

>Translated_434_residues
MPIITDVYAREVLDSRGNPTIEVEVFTESGALGRALVPSGASTGEHEAVELRDGDKDRYMGKGVLKAVENVNEIIAPEII
EGDFSVLDQVSIDKMMIALDGTDNKGKLGANAILGVSIAVARAAADYLGVPLYKYLGGFNGTELPVPMMNIVNGGSHSDA
PIAFQEFMVLPVGAPNFKEALRWGAEIFHNLAKILKGRNLSTAVGDEGGFAPTFEGTEDAVETILEAIKAAGLEPGKDVF
LGFDCAASEFFENGVYDYAKFEGENGKKRTSSEQVDYLEELVDKYPIITIEDGMDENDWEGWKALTDRLGNKVQLVGDDL
FVTNTKILERGINEGVGNSILIKVNQIGTLTETFEAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSR
TDRIAKYNQLLRIEDELFETAKFKGLNAFYNLNK
>Mature_433_residues
PIITDVYAREVLDSRGNPTIEVEVFTESGALGRALVPSGASTGEHEAVELRDGDKDRYMGKGVLKAVENVNEIIAPEIIE
GDFSVLDQVSIDKMMIALDGTDNKGKLGANAILGVSIAVARAAADYLGVPLYKYLGGFNGTELPVPMMNIVNGGSHSDAP
IAFQEFMVLPVGAPNFKEALRWGAEIFHNLAKILKGRNLSTAVGDEGGFAPTFEGTEDAVETILEAIKAAGLEPGKDVFL
GFDCAASEFFENGVYDYAKFEGENGKKRTSSEQVDYLEELVDKYPIITIEDGMDENDWEGWKALTDRLGNKVQLVGDDLF
VTNTKILERGINEGVGNSILIKVNQIGTLTETFEAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRT
DRIAKYNQLLRIEDELFETAKFKGLNAFYNLNK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.508120649652, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=50, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=428, Percent_Identity=50.4672897196262, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=428, Percent_Identity=50.4672897196262, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=44.8598130841121, Blast_Score=352, Evalue=6e-97,
Organism=Homo sapiens, GI169201331, Length=356, Percent_Identity=26.9662921348315, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI169201757, Length=356, Percent_Identity=26.9662921348315, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI239744207, Length=356, Percent_Identity=26.9662921348315, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI310129182, Length=139, Percent_Identity=30.9352517985612, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI310110045, Length=139, Percent_Identity=30.9352517985612, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI310120572, Length=139, Percent_Identity=30.9352517985612, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=59.9067599067599, Blast_Score=500, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=52.5345622119816, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=52.5345622119816, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=142, Percent_Identity=63.3802816901408, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=51.0204081632653, Blast_Score=417, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323985, Length=440, Percent_Identity=49.5454545454545, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=440, Percent_Identity=49.5454545454545, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324969, Length=440, Percent_Identity=49.5454545454545, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321968, Length=440, Percent_Identity=49.5454545454545, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=430, Percent_Identity=49.7674418604651, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=430, Percent_Identity=49.7674418604651, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=430, Percent_Identity=49.7674418604651, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=430, Percent_Identity=49.7674418604651, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=430, Percent_Identity=49.7674418604651, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=430, Percent_Identity=49.7674418604651, Blast_Score=392, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_MACCJ (B9EAH1)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559928.1
- ProteinModelPortal:   B9EAH1
- GeneID:   7389816
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0525
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46999; Mature: 46868

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 207-207 ACT_SITE 343-343 BINDING 157-157 BINDING 166-166 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIITDVYAREVLDSRGNPTIEVEVFTESGALGRALVPSGASTGEHEAVELRDGDKDRYM
CCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVNEIIAPEIIEGDFSVLDQVSIDKMMIALDGTDNKGKLGANAILGVSIAV
HHHHHHHHHHHHHHHCCHHCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHH
ARAAADYLGVPLYKYLGGFNGTELPVPMMNIVNGGSHSDAPIAFQEFMVLPVGAPNFKEA
HHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCEEEEHEEEEECCCCCHHHH
LRWGAEIFHNLAKILKGRNLSTAVGDEGGFAPTFEGTEDAVETILEAIKAAGLEPGKDVF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
LGFDCAASEFFENGVYDYAKFEGENGKKRTSSEQVDYLEELVDKYPIITIEDGMDENDWE
EEECHHHHHHHHCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCH
GWKALTDRLGNKVQLVGDDLFVTNTKILERGINEGVGNSILIKVNQIGTLTETFEAIEMA
HHHHHHHHCCCEEEEEECCEEEECHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHH
QKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFET
HHCCCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AKFKGLNAFYNLNK
HHHCCCHHEEECCC
>Mature Secondary Structure 
PIITDVYAREVLDSRGNPTIEVEVFTESGALGRALVPSGASTGEHEAVELRDGDKDRYM
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVNEIIAPEIIEGDFSVLDQVSIDKMMIALDGTDNKGKLGANAILGVSIAV
HHHHHHHHHHHHHHHCCHHCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHH
ARAAADYLGVPLYKYLGGFNGTELPVPMMNIVNGGSHSDAPIAFQEFMVLPVGAPNFKEA
HHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCEEEEHEEEEECCCCCHHHH
LRWGAEIFHNLAKILKGRNLSTAVGDEGGFAPTFEGTEDAVETILEAIKAAGLEPGKDVF
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEE
LGFDCAASEFFENGVYDYAKFEGENGKKRTSSEQVDYLEELVDKYPIITIEDGMDENDWE
EEECHHHHHHHHCCCCHHHEECCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCH
GWKALTDRLGNKVQLVGDDLFVTNTKILERGINEGVGNSILIKVNQIGTLTETFEAIEMA
HHHHHHHHCCCEEEEEECCEEEECHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHH
QKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFET
HHCCCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AKFKGLNAFYNLNK
HHHCCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA