Click here to switch to the map view.

The map label for this gene is yhcA [H]

Identifier: 222150672

GI number: 222150672

Start: 448458

End: 450236

Strand: Direct

Name: yhcA [H]

Synonym: MCCL_0422

Alternate gene names: 222150672

Gene position: 448458-450236 (Clockwise)

Preceding gene: 222150671

Following gene: 222150673

Centisome position: 21.33

GC content: 39.8

Gene sequence:

>1779_bases
ATGACCTCGATGATTATTTTATACATCTTATTTTCTTTGTTGACATTCTTCGTCGTTAATAGAGTAATGCTTAAAAGGAA
GCGCAAAGCTCAGCATAGCTCTATTCAAAAACAAGATAAACAAGTATATGAGCCAGTGTCTAACACACAAATTGAACCTA
CAACCGCAACGAAAGTAAGTGAACCACAAATACAAGATGAGCAAATGGAACAGGAAATAGATGTCAATCGTCCTGTACAT
GAACAGGTAAAAAAACCTGAAGCGCTTGTAACATTCGGTAAAGGTATCACCTTGACAAAGATTATTATTGCATTGATGGC
AGGGATGTTCGTTGCAATATTAAACCAGACATTAATAAACGTCGCTCTTCCTGTAATGATCAATGATTTCAGTATTTCTA
CTTCAACTGCACAATGGTTAACAACAGGGTTTATGCTTGTGAACGGTATTCTCGTTCCAGTCAGTGCTTATTTGATTCAG
AAATTTACGTATCGTCAGCTCTTTATGTTTGCAATGATTGCATTTACAATAGGCTCAGTTATCTGTGCTATAAGTACGAA
CTTCCCCGTTATGATGACAGGTCGTGTAATACAGGCGGTTGGAGCAGGAATCTTGATGCCGCTTGGAACAAACGTTTTTA
TGACGGTCTTCCCACCTGAGAAACGTGGAGCTGCAATGGGTATGATGGGGATTGCTTTCATTCTTGCACCTGCAATTGGA
CCGACATTAACAGGTTGGGTTATTCAGAATTATCATTGGAATGTGATGTTCTATGGTATGTCTGTTGTAGGAATACTCGC
AATTATTATAGGTTTCTTCTGGTTCAAAATCTATCAACCGATAAGTAATCCTAAGCTTGATGTTCCTGGTGTCATATTTA
GTTCGCTAGGATTTGGTAGTTTGCTTTATGGTTTTTCTGAAGCGGGAAATAAAGGCTGGGATTCGGGCATTGTAATTACA
ACTATGATTATTGGACTCTTATTCGTTGCATTATTTGTTTACAGAGAAATTTCTATGAAAGCTCCGATGATGGATCTACG
TGCTTTAAAGTATACGGGGTTTTCATTTACATTACTGATCAATGTAATCGTAACGATGAGTTTATTCGGAGGTATGTTGC
TTCTGCCTGTATATCTTCAGTCTATTCGAGGTTTTTCTCCTTTAGATTCAGGATTACTACTATTACCTGGCTCGCTGCTC
ATGGGACTCATGGGACCTATATCGGGTCGTCTGCTTGATAAATTTGGAATTAAGCCGATTGCGATATTCGGATTATTGAT
TATGACCTATGCAACATGGGAACTCACAAAGTTGAGCATGGATACTTCTTATTCAACGATATTAGGGATTTATGTACTGC
GTTCATTTGGTATGAGCTTTATTATGATGCCGATTATGACAGCAGGTATGAACGCATTACCACAAAGAATGATTCCGCAT
GGCAACGCCATCAGTAACACGGTACGTCAGCTTGCAGGGTCTATCGGTACTGCTGTACTGGTTACGATTATGACTCAACA
GACCACAGCGCATATGGGGGATATTGCCGATACGATGGATAAGACACATCCAGAAATTGCAGGACAGTTCGCTGAGATTG
GTCGCAGTGTTGGATCTCAAGAAGCGGGTGGTCAACTAGTGATGGGATATCTTCAGAAGTTCGCAACGATCAATGGTATT
AACGATGCCTTCTGGGTAGCGACAGCACTAAGTGCGTTAGCATTTATACTGTCCTTCTTCTTAAAAGGAAAAGAGCATTA
TCGTGCTGAAGAGCTTTAA

Upstream 100 bases:

>100_bases
CGTTCCCGTTAAGATTAAACTTTCAAAAGATAAATCTTTAGACATATTACCAGGAATGAATGTAACTGTACGCATTCATA
AAAACTAAGGAGGTTATCGT

Downstream 100 bases:

>100_bases
TTAAAAATAAACAAGTAAGGCATAATTATTTGTCATACTTGTTTATTTTTATATAATAAAGATTAAGGAGTGAGGCGATA
TGAAGACAAAGTATATTGAT

Product: drug resistance transporter EmrB/QacA subfamily protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MTSMIILYILFSLLTFFVVNRVMLKRKRKAQHSSIQKQDKQVYEPVSNTQIEPTTATKVSEPQIQDEQMEQEIDVNRPVH
EQVKKPEALVTFGKGITLTKIIIALMAGMFVAILNQTLINVALPVMINDFSISTSTAQWLTTGFMLVNGILVPVSAYLIQ
KFTYRQLFMFAMIAFTIGSVICAISTNFPVMMTGRVIQAVGAGILMPLGTNVFMTVFPPEKRGAAMGMMGIAFILAPAIG
PTLTGWVIQNYHWNVMFYGMSVVGILAIIIGFFWFKIYQPISNPKLDVPGVIFSSLGFGSLLYGFSEAGNKGWDSGIVIT
TMIIGLLFVALFVYREISMKAPMMDLRALKYTGFSFTLLINVIVTMSLFGGMLLLPVYLQSIRGFSPLDSGLLLLPGSLL
MGLMGPISGRLLDKFGIKPIAIFGLLIMTYATWELTKLSMDTSYSTILGIYVLRSFGMSFIMMPIMTAGMNALPQRMIPH
GNAISNTVRQLAGSIGTAVLVTIMTQQTTAHMGDIADTMDKTHPEIAGQFAEIGRSVGSQEAGGQLVMGYLQKFATINGI
NDAFWVATALSALAFILSFFLKGKEHYRAEEL

Sequences:

>Translated_592_residues
MTSMIILYILFSLLTFFVVNRVMLKRKRKAQHSSIQKQDKQVYEPVSNTQIEPTTATKVSEPQIQDEQMEQEIDVNRPVH
EQVKKPEALVTFGKGITLTKIIIALMAGMFVAILNQTLINVALPVMINDFSISTSTAQWLTTGFMLVNGILVPVSAYLIQ
KFTYRQLFMFAMIAFTIGSVICAISTNFPVMMTGRVIQAVGAGILMPLGTNVFMTVFPPEKRGAAMGMMGIAFILAPAIG
PTLTGWVIQNYHWNVMFYGMSVVGILAIIIGFFWFKIYQPISNPKLDVPGVIFSSLGFGSLLYGFSEAGNKGWDSGIVIT
TMIIGLLFVALFVYREISMKAPMMDLRALKYTGFSFTLLINVIVTMSLFGGMLLLPVYLQSIRGFSPLDSGLLLLPGSLL
MGLMGPISGRLLDKFGIKPIAIFGLLIMTYATWELTKLSMDTSYSTILGIYVLRSFGMSFIMMPIMTAGMNALPQRMIPH
GNAISNTVRQLAGSIGTAVLVTIMTQQTTAHMGDIADTMDKTHPEIAGQFAEIGRSVGSQEAGGQLVMGYLQKFATINGI
NDAFWVATALSALAFILSFFLKGKEHYRAEEL
>Mature_591_residues
TSMIILYILFSLLTFFVVNRVMLKRKRKAQHSSIQKQDKQVYEPVSNTQIEPTTATKVSEPQIQDEQMEQEIDVNRPVHE
QVKKPEALVTFGKGITLTKIIIALMAGMFVAILNQTLINVALPVMINDFSISTSTAQWLTTGFMLVNGILVPVSAYLIQK
FTYRQLFMFAMIAFTIGSVICAISTNFPVMMTGRVIQAVGAGILMPLGTNVFMTVFPPEKRGAAMGMMGIAFILAPAIGP
TLTGWVIQNYHWNVMFYGMSVVGILAIIIGFFWFKIYQPISNPKLDVPGVIFSSLGFGSLLYGFSEAGNKGWDSGIVITT
MIIGLLFVALFVYREISMKAPMMDLRALKYTGFSFTLLINVIVTMSLFGGMLLLPVYLQSIRGFSPLDSGLLLLPGSLLM
GLMGPISGRLLDKFGIKPIAIFGLLIMTYATWELTKLSMDTSYSTILGIYVLRSFGMSFIMMPIMTAGMNALPQRMIPHG
NAISNTVRQLAGSIGTAVLVTIMTQQTTAHMGDIADTMDKTHPEIAGQFAEIGRSVGSQEAGGQLVMGYLQKFATINGIN
DAFWVATALSALAFILSFFLKGKEHYRAEEL

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=493, Percent_Identity=24.9492900608519, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1788710, Length=436, Percent_Identity=27.7522935779816, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI87081983, Length=409, Percent_Identity=24.2053789731051, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1790195, Length=410, Percent_Identity=23.9024390243902, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1788392, Length=396, Percent_Identity=23.989898989899, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322958, Length=555, Percent_Identity=22.5225225225225, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=249, Percent_Identity=25.3012048192771, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 65017; Mature: 64885

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00014 ER_TARGET ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
6.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSMIILYILFSLLTFFVVNRVMLKRKRKAQHSSIQKQDKQVYEPVSNTQIEPTTATKVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EPQIQDEQMEQEIDVNRPVHEQVKKPEALVTFGKGITLTKIIIALMAGMFVAILNQTLIN
CCCCCHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VALPVMINDFSISTSTAQWLTTGFMLVNGILVPVSAYLIQKFTYRQLFMFAMIAFTIGSV
HHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICAISTNFPVMMTGRVIQAVGAGILMPLGTNVFMTVFPPEKRGAAMGMMGIAFILAPAIG
HHHHCCCCCEEEHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCC
PTLTGWVIQNYHWNVMFYGMSVVGILAIIIGFFWFKIYQPISNPKLDVPGVIFSSLGFGS
CHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHH
LLYGFSEAGNKGWDSGIVITTMIIGLLFVALFVYREISMKAPMMDLRALKYTGFSFTLLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
NVIVTMSLFGGMLLLPVYLQSIRGFSPLDSGLLLLPGSLLMGLMGPISGRLLDKFGIKPI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
AIFGLLIMTYATWELTKLSMDTSYSTILGIYVLRSFGMSFIMMPIMTAGMNALPQRMIPH
HHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
GNAISNTVRQLAGSIGTAVLVTIMTQQTTAHMGDIADTMDKTHPEIAGQFAEIGRSVGSQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
EAGGQLVMGYLQKFATINGINDAFWVATALSALAFILSFFLKGKEHYRAEEL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure 
TSMIILYILFSLLTFFVVNRVMLKRKRKAQHSSIQKQDKQVYEPVSNTQIEPTTATKVS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
EPQIQDEQMEQEIDVNRPVHEQVKKPEALVTFGKGITLTKIIIALMAGMFVAILNQTLIN
CCCCCHHHHHHHHCCCCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
VALPVMINDFSISTSTAQWLTTGFMLVNGILVPVSAYLIQKFTYRQLFMFAMIAFTIGSV
HHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ICAISTNFPVMMTGRVIQAVGAGILMPLGTNVFMTVFPPEKRGAAMGMMGIAFILAPAIG
HHHHCCCCCEEEHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCC
PTLTGWVIQNYHWNVMFYGMSVVGILAIIIGFFWFKIYQPISNPKLDVPGVIFSSLGFGS
CHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHH
LLYGFSEAGNKGWDSGIVITTMIIGLLFVALFVYREISMKAPMMDLRALKYTGFSFTLLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
NVIVTMSLFGGMLLLPVYLQSIRGFSPLDSGLLLLPGSLLMGLMGPISGRLLDKFGIKPI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHH
AIFGLLIMTYATWELTKLSMDTSYSTILGIYVLRSFGMSFIMMPIMTAGMNALPQRMIPH
HHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
GNAISNTVRQLAGSIGTAVLVTIMTQQTTAHMGDIADTMDKTHPEIAGQFAEIGRSVGSQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
EAGGQLVMGYLQKFATINGINDAFWVATALSALAFILSFFLKGKEHYRAEEL
CCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]