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The map label for this gene is adaB [H]

Identifier: 222150643

GI number: 222150643

Start: 422181

End: 422690

Strand: Direct

Name: adaB [H]

Synonym: MCCL_0393

Alternate gene names: 222150643

Gene position: 422181-422690 (Clockwise)

Preceding gene: 222150642

Following gene: 222150644

Centisome position: 20.08

GC content: 38.43

Gene sequence:

>510_bases
TTGAAAATTTATATAAGTTGTTTTCTCGTGCAGGATATCGAATTTATTATAGCTTCTGATGAATGTGGTGTCATATATTT
AGCGAATGAACATACACATGAATATGTCAACCGCCATATTGAACGTTTCTTCAGTCAAGCGACAAAAGTTACTGGCTATG
AAGCTGACCAGTACAATACGCATTATATTGAAGAGCTGAATCTTTATTTTGCAGGCAAGAAGCAGCAGTTAGAGTGGCCG
CTTAATCTTGCAGGGACAGACTTTCAAAAGTCTGTATGGCAGACGCTGCTTACGATTCCATATGGTGAAACACGATCGTA
TAGCGATATTGCATTGCAATCAGGTAATGTGAAAGCTGTCCGTGCAGTAGGAGGTGCAATTGGTGCAAATCCCGTGATGA
TTGTTGTGCCTTGTCATCGTGTTATCGGCAAGGATGGAACATTGACAGGATTTAGTGGTGGTTTAGATTTGAAGGATAAA
TTATTGAATATAGAGAAAATACAATTATAA

Upstream 100 bases:

>100_bases
TATCACCAGTATCTGATATTTATTTTGAAGATCTGATCCCCCATATAGATATGGCGCGAAAACCTTATGCAATAGATGCA
CAACTGGAGGGATGAATGAT

Downstream 100 bases:

>100_bases
AAATCAAAGGAGTAAGCTAATGACAATACCAGCATGTCCCAAATGTAATTCAGAATATACTTATGAAGATGGTCTAAACT
ATGTATGTCCAATGTGTGCT

Product: hypothetical protein

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MKIYISCFLVQDIEFIIASDECGVIYLANEHTHEYVNRHIERFFSQATKVTGYEADQYNTHYIEELNLYFAGKKQQLEWP
LNLAGTDFQKSVWQTLLTIPYGETRSYSDIALQSGNVKAVRAVGGAIGANPVMIVVPCHRVIGKDGTLTGFSGGLDLKDK
LLNIEKIQL

Sequences:

>Translated_169_residues
MKIYISCFLVQDIEFIIASDECGVIYLANEHTHEYVNRHIERFFSQATKVTGYEADQYNTHYIEELNLYFAGKKQQLEWP
LNLAGTDFQKSVWQTLLTIPYGETRSYSDIALQSGNVKAVRAVGGAIGANPVMIVVPCHRVIGKDGTLTGFSGGLDLKDK
LLNIEKIQL
>Mature_169_residues
MKIYISCFLVQDIEFIIASDECGVIYLANEHTHEYVNRHIERFFSQATKVTGYEADQYNTHYIEELNLYFAGKKQQLEWP
LNLAGTDFQKSVWQTLLTIPYGETRSYSDIALQSGNVKAVRAVGGAIGANPVMIVVPCHRVIGKDGTLTGFSGGLDLKDK
LLNIEKIQL

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=111, Percent_Identity=40.5405405405405, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=49.5145631067961, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788542, Length=111, Percent_Identity=44.1441441441441, Blast_Score=98, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=84, Percent_Identity=48.8095238095238, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=84, Percent_Identity=48.8095238095238, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=46.4285714285714, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18991; Mature: 18991

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYISCFLVQDIEFIIASDECGVIYLANEHTHEYVNRHIERFFSQATKVTGYEADQYNT
CEEEEEEEEEECEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HYIEELNLYFAGKKQQLEWPLNLAGTDFQKSVWQTLLTIPYGETRSYSDIALQSGNVKAV
EEEEEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEE
RAVGGAIGANPVMIVVPCHRVIGKDGTLTGFSGGLDLKDKLLNIEKIQL
EEECCCCCCCCEEEEEECHHHHCCCCCEECCCCCCCHHHHHCCEEEECC
>Mature Secondary Structure
MKIYISCFLVQDIEFIIASDECGVIYLANEHTHEYVNRHIERFFSQATKVTGYEADQYNT
CEEEEEEEEEECEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HYIEELNLYFAGKKQQLEWPLNLAGTDFQKSVWQTLLTIPYGETRSYSDIALQSGNVKAV
EEEEEEEEEEECCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEE
RAVGGAIGANPVMIVVPCHRVIGKDGTLTGFSGGLDLKDKLLNIEKIQL
EEECCCCCCCCEEEEEECHHHHCCCCCEECCCCCCCHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]