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The map label for this gene is ppsA [H]

Identifier: 222150536

GI number: 222150536

Start: 291133

End: 293706

Strand: Direct

Name: ppsA [H]

Synonym: MCCL_0286

Alternate gene names: 222150536

Gene position: 291133-293706 (Clockwise)

Preceding gene: 222150533

Following gene: 222150537

Centisome position: 13.85

GC content: 39.16

Gene sequence:

>2574_bases
ATGAAAGACTTATTAGGCGGTAAGGGTGCGAATCTTTCGGAGATGATGCGTCTCGGGTTGCCTGTACCTAATGGTTTCAC
GATTACGACTGAAGCTTGTATTGAATACCTAGCACAAGGTGAGCAGTTAACAGACGGTGTATTAAACGAATTACAGACAT
ATATTGAAGGTTTAGAAGATAAGACAGGTAAGGCTTTCTCTTCTAAAGAAAATTTATTACTTGTATCTGTACGTAGTGGT
GCGAAGATTTCGATGCCAGGAATGATGGATACAATTTTAAATTTAGGGTTAAACGACGAAAACGTTGAAAGTTTAGCACA
AGTGACGAACGATGCACGCTTTGCGTATGATTGCTACCGTCGTTTGCTACAGATGTTCGGAAGTGTTGTATACAATATCC
CAATGGAGGCCTTTGAAGGTTTCTTTGAATCATTCAAAAAAGAAAAAGGCCATCTGATTGATGCAGATATTCCTGCTGAA
GATTTAAAGGTGATTGTCGAGAAATATAAAGAAATTTACGTTGAAGAAGCATATAAGCCTTTCCCGCAAGATCCATTTAA
ACAGCTGACAGAAGCGATTGAAGCGGTATTCAAGTCATGGAATAATGATCGTGCGCGTATTTATCGTGACTTAAATGAAA
TTCCTCATGATATCGGTACAGCAGTGAACGTTCAGGAAATGGTCTTCGGAAACAGTGGAGACCGTTCAGGGACAGGTGTA
GCCTTTACACGCAATCCAGCGACTGGAGAACATAAATTATATGGAGAATATTTACTTAATGCACAAGGGGAAGATGTTGT
AGCAGGTATCCGTACTCCAAAAGAGATTGAAACATTAAACGAGCAGATGCCACAAGTGTATCAAGCATTCGTTGATGTCA
CGAAAAAATTAGAAGCGCATTATAAAGATATGCAGGATATTGAGTTCACCATTGAAAACGAAAAATTATTTATTCTACAG
ACGCGTAACGGTAAACGTACTGCCAAAGCTGCTATCAATATTGCTGTAGATATGGTACATGAAGGTGTAATTTCTAAAGA
AGACGCCGTTGCAATGGTAGATGTAAAGTCAATAGACCAGTTGTTACATCCTACCTTTAAGGAAGATGCATTAGAATCTG
CTGAATTAGTATCAGACAAAGGTTTACCAGCTTCTCCAGGTGCTGCAACAGGCATTATTGTCTTCTCGGCATTAGATGCT
AAATCAAAAGCTGAAGCGGGTGAAAAAGTTATTTTAGTTCGTCCAGAAACTTCTCCAGAGGATATTGAAGGGATGATTGC
AGCTGAAGCGATCGTAACGACACATGGGGGTATGACAAGTCACGCTGCTGTAGTTGCCCGTGGTATGGGCAAATGCTGTG
TAACTGGATGTTCAGATCTTGAAATTAACACGAAAGAAAAATATGTAAAATATGCCGGTAAAACGCTATCTGAAGGTGAT
GTTATCTCAGTTAACGGTTCGACGGGTGCTGTATACATCGGTGCAATTGAAACGACGAAAGCTGAAGCAAGTGATGCCTT
TGAAGAATTTATGGGCTGGGCCGAAGCAATTGCACGTTTATCTGTACGTATGAACGCTGAAACTGAAGGTGATATTACAG
CGGGTTATTCATTTAATGCACAAGGTATCGGTTTAGTACGTACTGAGCATATGTTCTTTGGTGAAGAGCGTTTAATCGAA
ATGAGACGTTTCATCTTAGCATCTACATACGAAGAGCGTGTAGAAGCTTTAAATAAAATTAAAGTGTATCAAACAGAAGA
TTTCGAAGGTATCTTTAATTTATCCGGGGACCGTCCATCAATCGTCCGTCTATTGGATCCACCATTGCATGAGTTCTTAC
CAAAAAGTGGGGAGGAAGTTAAAGCAGTTGCAGACCAGTTGGGTGTAACAGCAGACTTCTTAGAAAAACGAATTACTGAA
TTACATGAATTTAACCCGATGCTTGGTCACCGAGGTTGTCGATTAGCAATTACTTACCCTGAGTTATACGAAATGCAGAC
TGAAGCAATTATTACAGCTGTGCTTAATCTAAAACAAAGAGGAATTGAAACGAAACCAGAAATTATGATTCCATTAGTGT
CAACATCACAAGAATTCATTATCTTAAAAGAGATTGTTAAGAAAAAGGCTCAGGAAATCATGGATGCAAAAGGTGTACAA
GTTGACTATCTGATTGGTACAATGATTGAAACACCACGTGCATGCTTAGTAGCAGGCGAACTTGCTGAGCACTCAGAATT
CTTTAGTTTTGGTACAAATGATTTAGCACAATTAACATATGGTTTCTCTCGTGATGATGCAGGTAAATTTATTAACGAAT
ATATTAACCGTGAAATTCTTAGCGTCGATCCTTTCCAAACATTAGATGTAGAAGGTATGGGACAATTAATCAGGATTGCA
GTTGAAAGTGCGAAATCAGCAAATCCATCGATTAAAATCGGGGTTTGTGGTGAGTTAGGTGGAGATCCCAAATCAATCCA
TTTCTTTAATGGCTTAGATATTGATTATGTATCATGTTCACCATTCCGTGTACCAGGTGCAATTCTTGCAACAGCTCAAA
GTCAGGTGGAATAA

Upstream 100 bases:

>100_bases
ATTAGTATGACCTATTAAAACAAATGGTATAACACATTAAAAATAAGAGGTGAATGAAGTGAGTAAAAAAATTTATGCTT
TTGAAGAAGGTAAACAGAAT

Downstream 100 bases:

>100_bases
TATCGGGAGGCAGTACATTTGAGTAAGTATGAAGAAGATGATCTATTAATTTATATTGTTTCTGATTCTATCGGTGAAAC
AGCGGATAGAATGCTCCATG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 857; Mature: 857

Protein sequence:

>857_residues
MKDLLGGKGANLSEMMRLGLPVPNGFTITTEACIEYLAQGEQLTDGVLNELQTYIEGLEDKTGKAFSSKENLLLVSVRSG
AKISMPGMMDTILNLGLNDENVESLAQVTNDARFAYDCYRRLLQMFGSVVYNIPMEAFEGFFESFKKEKGHLIDADIPAE
DLKVIVEKYKEIYVEEAYKPFPQDPFKQLTEAIEAVFKSWNNDRARIYRDLNEIPHDIGTAVNVQEMVFGNSGDRSGTGV
AFTRNPATGEHKLYGEYLLNAQGEDVVAGIRTPKEIETLNEQMPQVYQAFVDVTKKLEAHYKDMQDIEFTIENEKLFILQ
TRNGKRTAKAAINIAVDMVHEGVISKEDAVAMVDVKSIDQLLHPTFKEDALESAELVSDKGLPASPGAATGIIVFSALDA
KSKAEAGEKVILVRPETSPEDIEGMIAAEAIVTTHGGMTSHAAVVARGMGKCCVTGCSDLEINTKEKYVKYAGKTLSEGD
VISVNGSTGAVYIGAIETTKAEASDAFEEFMGWAEAIARLSVRMNAETEGDITAGYSFNAQGIGLVRTEHMFFGEERLIE
MRRFILASTYEERVEALNKIKVYQTEDFEGIFNLSGDRPSIVRLLDPPLHEFLPKSGEEVKAVADQLGVTADFLEKRITE
LHEFNPMLGHRGCRLAITYPELYEMQTEAIITAVLNLKQRGIETKPEIMIPLVSTSQEFIILKEIVKKKAQEIMDAKGVQ
VDYLIGTMIETPRACLVAGELAEHSEFFSFGTNDLAQLTYGFSRDDAGKFINEYINREILSVDPFQTLDVEGMGQLIRIA
VESAKSANPSIKIGVCGELGGDPKSIHFFNGLDIDYVSCSPFRVPGAILATAQSQVE

Sequences:

>Translated_857_residues
MKDLLGGKGANLSEMMRLGLPVPNGFTITTEACIEYLAQGEQLTDGVLNELQTYIEGLEDKTGKAFSSKENLLLVSVRSG
AKISMPGMMDTILNLGLNDENVESLAQVTNDARFAYDCYRRLLQMFGSVVYNIPMEAFEGFFESFKKEKGHLIDADIPAE
DLKVIVEKYKEIYVEEAYKPFPQDPFKQLTEAIEAVFKSWNNDRARIYRDLNEIPHDIGTAVNVQEMVFGNSGDRSGTGV
AFTRNPATGEHKLYGEYLLNAQGEDVVAGIRTPKEIETLNEQMPQVYQAFVDVTKKLEAHYKDMQDIEFTIENEKLFILQ
TRNGKRTAKAAINIAVDMVHEGVISKEDAVAMVDVKSIDQLLHPTFKEDALESAELVSDKGLPASPGAATGIIVFSALDA
KSKAEAGEKVILVRPETSPEDIEGMIAAEAIVTTHGGMTSHAAVVARGMGKCCVTGCSDLEINTKEKYVKYAGKTLSEGD
VISVNGSTGAVYIGAIETTKAEASDAFEEFMGWAEAIARLSVRMNAETEGDITAGYSFNAQGIGLVRTEHMFFGEERLIE
MRRFILASTYEERVEALNKIKVYQTEDFEGIFNLSGDRPSIVRLLDPPLHEFLPKSGEEVKAVADQLGVTADFLEKRITE
LHEFNPMLGHRGCRLAITYPELYEMQTEAIITAVLNLKQRGIETKPEIMIPLVSTSQEFIILKEIVKKKAQEIMDAKGVQ
VDYLIGTMIETPRACLVAGELAEHSEFFSFGTNDLAQLTYGFSRDDAGKFINEYINREILSVDPFQTLDVEGMGQLIRIA
VESAKSANPSIKIGVCGELGGDPKSIHFFNGLDIDYVSCSPFRVPGAILATAQSQVE
>Mature_857_residues
MKDLLGGKGANLSEMMRLGLPVPNGFTITTEACIEYLAQGEQLTDGVLNELQTYIEGLEDKTGKAFSSKENLLLVSVRSG
AKISMPGMMDTILNLGLNDENVESLAQVTNDARFAYDCYRRLLQMFGSVVYNIPMEAFEGFFESFKKEKGHLIDADIPAE
DLKVIVEKYKEIYVEEAYKPFPQDPFKQLTEAIEAVFKSWNNDRARIYRDLNEIPHDIGTAVNVQEMVFGNSGDRSGTGV
AFTRNPATGEHKLYGEYLLNAQGEDVVAGIRTPKEIETLNEQMPQVYQAFVDVTKKLEAHYKDMQDIEFTIENEKLFILQ
TRNGKRTAKAAINIAVDMVHEGVISKEDAVAMVDVKSIDQLLHPTFKEDALESAELVSDKGLPASPGAATGIIVFSALDA
KSKAEAGEKVILVRPETSPEDIEGMIAAEAIVTTHGGMTSHAAVVARGMGKCCVTGCSDLEINTKEKYVKYAGKTLSEGD
VISVNGSTGAVYIGAIETTKAEASDAFEEFMGWAEAIARLSVRMNAETEGDITAGYSFNAQGIGLVRTEHMFFGEERLIE
MRRFILASTYEERVEALNKIKVYQTEDFEGIFNLSGDRPSIVRLLDPPLHEFLPKSGEEVKAVADQLGVTADFLEKRITE
LHEFNPMLGHRGCRLAITYPELYEMQTEAIITAVLNLKQRGIETKPEIMIPLVSTSQEFIILKEIVKKKAQEIMDAKGVQ
VDYLIGTMIETPRACLVAGELAEHSEFFSFGTNDLAQLTYGFSRDDAGKFINEYINREILSVDPFQTLDVEGMGQLIRIA
VESAKSANPSIKIGVCGELGGDPKSIHFFNGLDIDYVSCSPFRVPGAILATAQSQVE

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=902, Percent_Identity=23.3924611973392, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1788756, Length=454, Percent_Identity=23.568281938326, Blast_Score=97, Evalue=6e-21,
Organism=Escherichia coli, GI1789193, Length=506, Percent_Identity=22.3320158102767, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI48994992, Length=442, Percent_Identity=24.4343891402715, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1788726, Length=602, Percent_Identity=21.2624584717608, Blast_Score=84, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 94446; Mature: 94446

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLLGGKGANLSEMMRLGLPVPNGFTITTEACIEYLAQGEQLTDGVLNELQTYIEGLED
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
KTGKAFSSKENLLLVSVRSGAKISMPGMMDTILNLGLNDENVESLAQVTNDARFAYDCYR
CCCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHH
RLLQMFGSVVYNIPMEAFEGFFESFKKEKGHLIDADIPAEDLKVIVEKYKEIYVEEAYKP
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
FPQDPFKQLTEAIEAVFKSWNNDRARIYRDLNEIPHDIGTAVNVQEMVFGNSGDRSGTGV
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCE
AFTRNPATGEHKLYGEYLLNAQGEDVVAGIRTPKEIETLNEQMPQVYQAFVDVTKKLEAH
EEECCCCCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKDMQDIEFTIENEKLFILQTRNGKRTAKAAINIAVDMVHEGVISKEDAVAMVDVKSIDQ
HCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHH
LLHPTFKEDALESAELVSDKGLPASPGAATGIIVFSALDAKSKAEAGEKVILVRPETSPE
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEECCCCCHH
DIEGMIAAEAIVTTHGGMTSHAAVVARGMGKCCVTGCSDLEINTKEKYVKYAGKTLSEGD
HHHHHHHHHHHHEECCCCHHHHHHHHHCCCHHHHCCCCCCEECHHHHHHHHHCCCCCCCC
VISVNGSTGAVYIGAIETTKAEASDAFEEFMGWAEAIARLSVRMNAETEGDITAGYSFNA
EEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEECEECCC
QGIGLVRTEHMFFGEERLIEMRRFILASTYEERVEALNKIKVYQTEDFEGIFNLSGDRPS
CCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECCCCCCC
IVRLLDPPLHEFLPKSGEEVKAVADQLGVTADFLEKRITELHEFNPMLGHRGCRLAITYP
CHHHCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECH
ELYEMQTEAIITAVLNLKQRGIETKPEIMIPLVSTSQEFIILKEIVKKKAQEIMDAKGVQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCE
VDYLIGTMIETPRACLVAGELAEHSEFFSFGTNDLAQLTYGFSRDDAGKFINEYINREIL
EEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCEE
SVDPFQTLDVEGMGQLIRIAVESAKSANPSIKIGVCGELGGDPKSIHFFNGLDIDYVSCS
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCEEEECCC
PFRVPGAILATAQSQVE
CCCCCCHHHHCCHHHCC
>Mature Secondary Structure
MKDLLGGKGANLSEMMRLGLPVPNGFTITTEACIEYLAQGEQLTDGVLNELQTYIEGLED
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCC
KTGKAFSSKENLLLVSVRSGAKISMPGMMDTILNLGLNDENVESLAQVTNDARFAYDCYR
CCCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHH
RLLQMFGSVVYNIPMEAFEGFFESFKKEKGHLIDADIPAEDLKVIVEKYKEIYVEEAYKP
HHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCC
FPQDPFKQLTEAIEAVFKSWNNDRARIYRDLNEIPHDIGTAVNVQEMVFGNSGDRSGTGV
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCE
AFTRNPATGEHKLYGEYLLNAQGEDVVAGIRTPKEIETLNEQMPQVYQAFVDVTKKLEAH
EEECCCCCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YKDMQDIEFTIENEKLFILQTRNGKRTAKAAINIAVDMVHEGVISKEDAVAMVDVKSIDQ
HCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHH
LLHPTFKEDALESAELVSDKGLPASPGAATGIIVFSALDAKSKAEAGEKVILVRPETSPE
HHCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCHHHHCCCCEEEEECCCCCHH
DIEGMIAAEAIVTTHGGMTSHAAVVARGMGKCCVTGCSDLEINTKEKYVKYAGKTLSEGD
HHHHHHHHHHHHEECCCCHHHHHHHHHCCCHHHHCCCCCCEECHHHHHHHHHCCCCCCCC
VISVNGSTGAVYIGAIETTKAEASDAFEEFMGWAEAIARLSVRMNAETEGDITAGYSFNA
EEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEECEECCC
QGIGLVRTEHMFFGEERLIEMRRFILASTYEERVEALNKIKVYQTEDFEGIFNLSGDRPS
CCCEEEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEECCCCCCC
IVRLLDPPLHEFLPKSGEEVKAVADQLGVTADFLEKRITELHEFNPMLGHRGCRLAITYP
CHHHCCCCHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECH
ELYEMQTEAIITAVLNLKQRGIETKPEIMIPLVSTSQEFIILKEIVKKKAQEIMDAKGVQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCE
VDYLIGTMIETPRACLVAGELAEHSEFFSFGTNDLAQLTYGFSRDDAGKFINEYINREIL
EEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCEE
SVDPFQTLDVEGMGQLIRIAVESAKSANPSIKIGVCGELGGDPKSIHFFNGLDIDYVSCS
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEECCCCEEEECCC
PFRVPGAILATAQSQVE
CCCCCCHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]