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The map label for this gene is rpoA

Identifier: 222150471

GI number: 222150471

Start: 216196

End: 217140

Strand: Direct

Name: rpoA

Synonym: MCCL_0221

Alternate gene names: 222150471

Gene position: 216196-217140 (Clockwise)

Preceding gene: 222150470

Following gene: 222150472

Centisome position: 10.28

GC content: 33.97

Gene sequence:

>945_bases
ATGATTGAAATCGAAAAGCCTAGAATTGAGACAGTAGAAATTAGTGAAGATTCTAAATTCGGTAAATTCGTAGTTGAACC
GTTAGAACGTGGTTATGGTACAACATTAGGAAACTCCTTACGTCGTATCTTATTATCTTCATTACCAGGTGCGGCTGTTA
AGAACATTGAAATCGAAGGCGTATTACATGAATTTTCAGCAATTGAAAACGTTGTTGAAGATGTTACATCAATTATCATG
AACATAAAGAAGCTAGCGCTTAAAATCTATTCTGATGAAGATAAAACGTTAGAAATCGATGTTAAAGATGAAGGTGTCGT
TACTGCTAAAGATATCATGCACGACAGCGATGTTGAGATTTTAAATCCAGATTTAAAAATTGCAACAGTATCTAAAGGTG
GACACTTAAAAATGCGTTTAATTGCTAATGCAGGGCGCGGTTATACTTTAGCTGAAGATAATAATACAAGTGATTTACCA
ATTGGGGTTATTCCAGTTGATTCAATCTACACACCAGTTGAGCGTGTAAACTATCAAGTTGAGAATACACGTGTAGGTCA
AAGTACGAACTTTGACAAATTAACTTTAGATGTATGGACTGACGGATCAATAACACCACAAGAAGCAATTTCTTTAGGTG
CTAAGATTATGACAGAACATTTAAATATCTTCGTTGATTTAACTGATGAAGCACAAAATGCTGAAATCATGATTGAAAAA
GAAGAAGATCACAAAGAAAAAGTTCTAGAGATGTCTATAGAAGAACTTGATTTATCAGTTCGTTCTTATAACTGTCTGAA
ACGTGCAGGTATTAATTCTGTTCAGGAATTAGCTGACAAATCAGAAGCTGATATGATGAAAGTTCGTAACTTAGGACGCA
AGTCTTTAGAAGAAGTTAAGTTTAAATTAGAAGACTTAGGTCTTGGATTAAGAAAAGAAGACTGA

Upstream 100 bases:

>100_bases
CGAAACGTCGTCGCGTATAATTTTGAATTAGTATCAAAGACACTTATAATAGTATATAGGACTAAAAAAATTATTCGACG
TTGTTGAAGGAGGATATATA

Downstream 100 bases:

>100_bases
TAAAGGAGGTTACGATTCATGGGTTACAGAAAATTAGGACGTACGTCTGATCAACGTAAAGCGATGTTACGCGACTTAGC
AACTTCTTTAATCGTTAACG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIEIEKPRIETVEISEDSKFGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVKNIEIEGVLHEFSAIENVVEDVTSIIM
NIKKLALKIYSDEDKTLEIDVKDEGVVTAKDIMHDSDVEILNPDLKIATVSKGGHLKMRLIANAGRGYTLAEDNNTSDLP
IGVIPVDSIYTPVERVNYQVENTRVGQSTNFDKLTLDVWTDGSITPQEAISLGAKIMTEHLNIFVDLTDEAQNAEIMIEK
EEDHKEKVLEMSIEELDLSVRSYNCLKRAGINSVQELADKSEADMMKVRNLGRKSLEEVKFKLEDLGLGLRKED

Sequences:

>Translated_314_residues
MIEIEKPRIETVEISEDSKFGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVKNIEIEGVLHEFSAIENVVEDVTSIIM
NIKKLALKIYSDEDKTLEIDVKDEGVVTAKDIMHDSDVEILNPDLKIATVSKGGHLKMRLIANAGRGYTLAEDNNTSDLP
IGVIPVDSIYTPVERVNYQVENTRVGQSTNFDKLTLDVWTDGSITPQEAISLGAKIMTEHLNIFVDLTDEAQNAEIMIEK
EEDHKEKVLEMSIEELDLSVRSYNCLKRAGINSVQELADKSEADMMKVRNLGRKSLEEVKFKLEDLGLGLRKED
>Mature_314_residues
MIEIEKPRIETVEISEDSKFGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVKNIEIEGVLHEFSAIENVVEDVTSIIM
NIKKLALKIYSDEDKTLEIDVKDEGVVTAKDIMHDSDVEILNPDLKIATVSKGGHLKMRLIANAGRGYTLAEDNNTSDLP
IGVIPVDSIYTPVERVNYQVENTRVGQSTNFDKLTLDVWTDGSITPQEAISLGAKIMTEHLNIFVDLTDEAQNAEIMIEK
EEDHKEKVLEMSIEELDLSVRSYNCLKRAGINSVQELADKSEADMMKVRNLGRKSLEEVKFKLEDLGLGLRKED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=318, Percent_Identity=42.7672955974843, Blast_Score=239, Evalue=1e-64,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_MACCJ (B9E9L7)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559624.1
- GeneID:   7389558
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0221
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 35060; Mature: 35060

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIEKPRIETVEISEDSKFGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVKNIEIEG
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHCCEEHHH
VLHEFSAIENVVEDVTSIIMNIKKLALKIYSDEDKTLEIDVKDEGVVTAKDIMHDSDVEI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCEEEHHHHCCCCCCEE
LNPDLKIATVSKGGHLKMRLIANAGRGYTLAEDNNTSDLPIGVIPVDSIYTPVERVNYQV
ECCCEEEEEECCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEEECCHHHCCHHHCCEEE
ENTRVGQSTNFDKLTLDVWTDGSITPQEAISLGAKIMTEHLNIFVDLTDEAQNAEIMIEK
ECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEEEEC
EEDHKEKVLEMSIEELDLSVRSYNCLKRAGINSVQELADKSEADMMKVRNLGRKSLEEVK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
FKLEDLGLGLRKED
HHHHHCCCCCCCCC
>Mature Secondary Structure
MIEIEKPRIETVEISEDSKFGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVKNIEIEG
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHCCEEHHH
VLHEFSAIENVVEDVTSIIMNIKKLALKIYSDEDKTLEIDVKDEGVVTAKDIMHDSDVEI
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCEEEHHHHCCCCCCEE
LNPDLKIATVSKGGHLKMRLIANAGRGYTLAEDNNTSDLPIGVIPVDSIYTPVERVNYQV
ECCCEEEEEECCCCCEEEEEEECCCCCEEEECCCCCCCCEEEEEECCHHHCCHHHCCEEE
ENTRVGQSTNFDKLTLDVWTDGSITPQEAISLGAKIMTEHLNIFVDLTDEAQNAEIMIEK
ECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCEEEEEEEECCCCCCCEEEEEC
EEDHKEKVLEMSIEELDLSVRSYNCLKRAGINSVQELADKSEADMMKVRNLGRKSLEEVK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
FKLEDLGLGLRKED
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA