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The map label for this gene is mmsB [H]

Identifier: 222150428

GI number: 222150428

Start: 183817

End: 184674

Strand: Direct

Name: mmsB [H]

Synonym: MCCL_0178

Alternate gene names: 222150428

Gene position: 183817-184674 (Clockwise)

Preceding gene: 222150426

Following gene: 222150429

Centisome position: 8.74

GC content: 37.88

Gene sequence:

>858_bases
ATGAAAATTGGTTTTATTGGAACAGGCGTAATGGGCAAAAATATGGCTGAGCATATAGGTGATAAACTATATATTTATAA
CCGAACGAAAGAAAAAGCAAGGTCACTAATCGAACAAGGTCATATATGGTGCGATACACCTGCTGAAGTTGCAGCACAAT
CCGATATTATTTTTACTATGCTTGGCTATCCAGCTGATGTAGAAACTATCTATCTCGCACCAGATGGATTGATTAATAAT
GGTAAACAAGGTCAAATATTTATTGACTGTACAACGAGTAGTCCTGAACTTGCCCAAAAGATCAATCAAGAAGCAAATAA
GAAAGGAATATATGTGTTAGACGCACCTGTAAGTGGTGGAGATATCGGTGCCAAGAACGGAACTTTGAGTGTAATGGTGG
GTGGAGATGAAGCGATATTTGATAGAGTAAAACCCTTAATCGATAAATTTAGTAGTGCAGTAACATATTTTGGTGATGCA
GGAAGTGGTCAACATACTAAGATGGCGAATCAAATTGCTATCGCTACAAATATGATCGGTATGGCTGAAAGTCTGTACTA
TGCGCATAGAGCAGGATTAGATGTTGAAAAAGTACTAGAAACAATCAGTAAAGGAGCGGCAGGAAGCTGGTCATTATCTC
ACTTAGCTCCTAGAATTTTAAAAGAAGACTACGCGCCTGGATTTTATACGCATCACTTCCTGAAAGATATGAGTATCGCG
CTTGAAGAAACAAAGAAGATGGGCATTGAACTTCCGGGTCTCGAATTAAGCTGTAAGTTATATAGTAGCCTTTCCGATGA
ACTAAAAGAAACGACAGGTACACATGTGATATATCAATATTATACACAAGCAAGATAA

Upstream 100 bases:

>100_bases
AAAAACATAATTACTTTTTGCATTCTTTTCACTCCTTTCCCTATAATTGTAATATATAACTGTAAATTATCAATATAATA
TGACAAGAGAGGTTTTCAAA

Downstream 100 bases:

>100_bases
GAATATTGGTAAAATTTAAATAATAAAGCTACAATAAACACATAAAATTCGTATAAATGGAGGAAACCCTTTGATAAAGA
AAATTATTGATTTTTCTTTG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MKIGFIGTGVMGKNMAEHIGDKLYIYNRTKEKARSLIEQGHIWCDTPAEVAAQSDIIFTMLGYPADVETIYLAPDGLINN
GKQGQIFIDCTTSSPELAQKINQEANKKGIYVLDAPVSGGDIGAKNGTLSVMVGGDEAIFDRVKPLIDKFSSAVTYFGDA
GSGQHTKMANQIAIATNMIGMAESLYYAHRAGLDVEKVLETISKGAAGSWSLSHLAPRILKEDYAPGFYTHHFLKDMSIA
LEETKKMGIELPGLELSCKLYSSLSDELKETTGTHVIYQYYTQAR

Sequences:

>Translated_285_residues
MKIGFIGTGVMGKNMAEHIGDKLYIYNRTKEKARSLIEQGHIWCDTPAEVAAQSDIIFTMLGYPADVETIYLAPDGLINN
GKQGQIFIDCTTSSPELAQKINQEANKKGIYVLDAPVSGGDIGAKNGTLSVMVGGDEAIFDRVKPLIDKFSSAVTYFGDA
GSGQHTKMANQIAIATNMIGMAESLYYAHRAGLDVEKVLETISKGAAGSWSLSHLAPRILKEDYAPGFYTHHFLKDMSIA
LEETKKMGIELPGLELSCKLYSSLSDELKETTGTHVIYQYYTQAR
>Mature_285_residues
MKIGFIGTGVMGKNMAEHIGDKLYIYNRTKEKARSLIEQGHIWCDTPAEVAAQSDIIFTMLGYPADVETIYLAPDGLINN
GKQGQIFIDCTTSSPELAQKINQEANKKGIYVLDAPVSGGDIGAKNGTLSVMVGGDEAIFDRVKPLIDKFSSAVTYFGDA
GSGQHTKMANQIAIATNMIGMAESLYYAHRAGLDVEKVLETISKGAAGSWSLSHLAPRILKEDYAPGFYTHHFLKDMSIA
LEETKKMGIELPGLELSCKLYSSLSDELKETTGTHVIYQYYTQAR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=250, Percent_Identity=32.8, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI40556376, Length=243, Percent_Identity=25.9259259259259, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI145693186, Length=255, Percent_Identity=38.4313725490196, Blast_Score=183, Evalue=9e-48,
Organism=Escherichia coli, GI1786719, Length=268, Percent_Identity=36.1940298507463, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790315, Length=265, Percent_Identity=27.1698113207547, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789092, Length=269, Percent_Identity=24.5353159851301, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557316, Length=289, Percent_Identity=27.3356401384083, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=32.0945945945946, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=32.0945945945946, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI28574115, Length=263, Percent_Identity=20.532319391635, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31089; Mature: 31089

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGFIGTGVMGKNMAEHIGDKLYIYNRTKEKARSLIEQGHIWCDTPAEVAAQSDIIFTM
CEEEEEECCCCCCHHHHHCCCEEEEEECCHHHHHHHHHCCCEEECCHHHHHCCCCEEEEE
LGYPADVETIYLAPDGLINNGKQGQIFIDCTTSSPELAQKINQEANKKGIYVLDAPVSGG
ECCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCC
DIGAKNGTLSVMVGGDEAIFDRVKPLIDKFSSAVTYFGDAGSGQHTKMANQIAIATNMIG
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHH
MAESLYYAHRAGLDVEKVLETISKGAAGSWSLSHLAPRILKEDYAPGFYTHHFLKDMSIA
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEETKKMGIELPGLELSCKLYSSLSDELKETTGTHVIYQYYTQAR
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCC
>Mature Secondary Structure
MKIGFIGTGVMGKNMAEHIGDKLYIYNRTKEKARSLIEQGHIWCDTPAEVAAQSDIIFTM
CEEEEEECCCCCCHHHHHCCCEEEEEECCHHHHHHHHHCCCEEECCHHHHHCCCCEEEEE
LGYPADVETIYLAPDGLINNGKQGQIFIDCTTSSPELAQKINQEANKKGIYVLDAPVSGG
ECCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCC
DIGAKNGTLSVMVGGDEAIFDRVKPLIDKFSSAVTYFGDAGSGQHTKMANQIAIATNMIG
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHH
MAESLYYAHRAGLDVEKVLETISKGAAGSWSLSHLAPRILKEDYAPGFYTHHFLKDMSIA
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LEETKKMGIELPGLELSCKLYSSLSDELKETTGTHVIYQYYTQAR
HHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]