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The map label for this gene is narI [H]

Identifier: 222150389

GI number: 222150389

Start: 145798

End: 146472

Strand: Direct

Name: narI [H]

Synonym: MCCL_0139

Alternate gene names: 222150389

Gene position: 145798-146472 (Clockwise)

Preceding gene: 222150388

Following gene: 222150390

Centisome position: 6.94

GC content: 37.33

Gene sequence:

>675_bases
ATGGGTGATCAGTTATTATGGGTAATATTCCCTTATGCGTGTATTGCGATTTTTATTATCGGTCATATTTTTAGATTTAA
ATATGATCAGTTTTCCTGGACGGCAAAGTCAAGTGAATTTGTAGAGAAGAAGTCGTTGATGTGGGGAAGTATTTTATTCC
ACTTAGGTATTATCCCAGTTATATTAGGGCACGTCGTAGGTCTAGGTATTCCGGCCAGCTGGTTACGTGCGATTGGTGTG
AGCGATCATTTATATCACATCGGGGCGGTATATATCGGAAGCATCTTCGGTATCGTGACGCTTATCGGTATGTTGTTACT
GACAGCACGTCGTGTTACAAATGAAAACGTAAGAAAGTTAAGTTCGTTATCAGATATACTTGTTAACTTTCTATTACTAT
TCATCGTATTTATCGGCTGCTATTCAACAATCGTAACGAATGCTACATTACCAGACTTTGATTATCGTAATACAATCTCT
GAATGGTTCCGTGGATTATTAATATTAAGACCTGAAGCAGGATATATGGAAGGTGTTCCATTATCTTTCAAGATTCACGT
AATCGCAGGATTCTTAATTACAGCATTGTGGCCATTTACTCGTCTTGTACATGTATGGAGTGTACCTGTAAACTATGTTG
GACGCAGTCACATTATATATCGTAAACATAAATAA

Upstream 100 bases:

>100_bases
GCATTAGATCACATTAAGAGCCCATATTATCATTTAATCTTAGCGATAAGAGAAACATGTAAAGCATGTTTGATTAAAGA
TGAGAATGAGGTGACGTTAA

Downstream 100 bases:

>100_bases
TAAGTTATAATCAGAAGGCAAAGGAGTGTAAATGATGCATGATTATCAAGCGATGATAGACCAGCTTAGAGAATCACTGA
ATGTAGATTTTATCGGACTC

Product: nitrate reductase gamma chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MGDQLLWVIFPYACIAIFIIGHIFRFKYDQFSWTAKSSEFVEKKSLMWGSILFHLGIIPVILGHVVGLGIPASWLRAIGV
SDHLYHIGAVYIGSIFGIVTLIGMLLLTARRVTNENVRKLSSLSDILVNFLLLFIVFIGCYSTIVTNATLPDFDYRNTIS
EWFRGLLILRPEAGYMEGVPLSFKIHVIAGFLITALWPFTRLVHVWSVPVNYVGRSHIIYRKHK

Sequences:

>Translated_224_residues
MGDQLLWVIFPYACIAIFIIGHIFRFKYDQFSWTAKSSEFVEKKSLMWGSILFHLGIIPVILGHVVGLGIPASWLRAIGV
SDHLYHIGAVYIGSIFGIVTLIGMLLLTARRVTNENVRKLSSLSDILVNFLLLFIVFIGCYSTIVTNATLPDFDYRNTIS
EWFRGLLILRPEAGYMEGVPLSFKIHVIAGFLITALWPFTRLVHVWSVPVNYVGRSHIIYRKHK
>Mature_223_residues
GDQLLWVIFPYACIAIFIIGHIFRFKYDQFSWTAKSSEFVEKKSLMWGSILFHLGIIPVILGHVVGLGIPASWLRAIGVS
DHLYHIGAVYIGSIFGIVTLIGMLLLTARRVTNENVRKLSSLSDILVNFLLLFIVFIGCYSTIVTNATLPDFDYRNTISE
WFRGLLILRPEAGYMEGVPLSFKIHVIAGFLITALWPFTRLVHVWSVPVNYVGRSHIIYRKHK

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Trans

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=217, Percent_Identity=35.9447004608295, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1787480, Length=214, Percent_Identity=32.2429906542056, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25457; Mature: 25325

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDQLLWVIFPYACIAIFIIGHIFRFKYDQFSWTAKSSEFVEKKSLMWGSILFHLGIIPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ILGHVVGLGIPASWLRAIGVSDHLYHIGAVYIGSIFGIVTLIGMLLLTARRVTNENVRKL
HHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSLSDILVNFLLLFIVFIGCYSTIVTNATLPDFDYRNTISEWFRGLLILRPEAGYMEGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCC
LSFKIHVIAGFLITALWPFTRLVHVWSVPVNYVGRSHIIYRKHK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCC
>Mature Secondary Structure 
GDQLLWVIFPYACIAIFIIGHIFRFKYDQFSWTAKSSEFVEKKSLMWGSILFHLGIIPV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ILGHVVGLGIPASWLRAIGVSDHLYHIGAVYIGSIFGIVTLIGMLLLTARRVTNENVRKL
HHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSLSDILVNFLLLFIVFIGCYSTIVTNATLPDFDYRNTISEWFRGLLILRPEAGYMEGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEECCCCCCCCCCC
LSFKIHVIAGFLITALWPFTRLVHVWSVPVNYVGRSHIIYRKHK
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]