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The map label for this gene is narH [H]

Identifier: 222150387

GI number: 222150387

Start: 143684

End: 145234

Strand: Direct

Name: narH [H]

Synonym: MCCL_0137

Alternate gene names: 222150387

Gene position: 143684-145234 (Clockwise)

Preceding gene: 222150386

Following gene: 222150388

Centisome position: 6.83

GC content: 40.81

Gene sequence:

>1551_bases
TTGAAGATTAAAGCACAAGTCGCAATGGTAATGAATTTAGATAAATGTATCGGATGCCATACATGTAGTATTACATGTAA
AAGTACCTGGACGAATCGTCCAGGTGCTGAATACATGTGGTTTAACAACGTAGAGACAAAACCAGGTATCGGCTATCCGA
AGCGCTGGGAAGACCAGGAAGTATATAAAGGTGGCTGGCAGTTAAACAGCAAAGGAAAATTAGAGCTTAAATCAGGTTCT
AAACTTTCTAAAATTGCTTTAGGAAAAATCTTCTACAACCCAGATATGCCTGAAATGAAAGACTATTATGAGCCATGGAC
TTACAACTATGCTAATCTTACGAACGCGAAAGAACAAAAGCATTCACCAGTAGCAACGGCTGAATCATTAGTATCAGGTA
AAAAAATGGACTTACAGTGGGGACCGAACTGGGAAGATGACTTAGCTGGTGCGCACATCACAGGACCAACCGATCCAAAC
ATTCAAAAGATTGAAGAAGAAATTAAGTTCAATTTTGAACAGTCGTTTATGATGTACTTACCACGTCTATGTGAACATTG
TTTAAATCCATCTTGTGTGGCAAGTTGTCCGAGCGGTGCAATGTATAAACGTGATGAAGATGGTATCGTACTTGTAGACC
AAGATGCTTGTCGTGGATGGAGATACTGTATGACAGGTTGCCCTTACAAAAAAGTATACTTCAACTGGAAAACGAATAAA
GCTGAGAAATGTACCTTCTGTTTCCCTCGTGTTGAAGCAGGACTTCCGACAGTATGTTCTGAAACATGTACGGGACGTAT
GCGTTATTTAGGTGTATTACTGTATGATGCTGACCGTGTATTAGAAGCAGCGACTGTAAAGGATCCACAAGATCTATATC
AAGCACAACTCGATTTATTCTTAGATCCTCATGACCCAGAAGTGATTGCTCAAGCAGAGCGTGATGGTATTGCACAAGAC
TGGATTGAAGCAGCACAAAACTCACCAGTATATAAACTTGCAATTGAATATAAACTTGCATTCCCATTACATCCTGAATA
TCGTACATTACCAATGGTGTGGTACTGCCCACCACTTAGCCCGATTATGAACTACTTTGAAGGTAAAGATTCTATCAAGA
ACCCAGATATGATCTTCCCTGCAATTGAAGAGATGCGTCTACCAATTCAATACTTAGCAAACATGTTAACCGCAGGAGAT
GCACAAACTGTAAAAGAAGCATTACAACGTATGGCAATGATGCGTAGTTACATGAGAGCCGTTTCAAGTGGTAAAGACTT
TGACGCGTCGCGTTTAGACCGTGTAGGCATGACTGCACATCAAGTGAAACAGATGTATCGATTACTTGCGATTGCAAAGC
ATGAAGACCGCTTTGTCATCCCGACATCACATAAAGAAGGTTACATGAATACGTATAGCGCTCAAGGTGGCGAGGGTTAT
ACAAGTGATAACTTCGGTGCAGATTGTGATGGTTGTGGACCAGTACCTGCTGGTAAGTCTGGTAAAGAAGTTTATGAAGA
TAACTTCTACGGAGGCATTTGGCGTGATTGA

Upstream 100 bases:

>100_bases
GGTTATGCACAAATTAGTTACGGATTTAACTACTATGGTCCAATTGGTAACCAACGTGATGTTTACGTTGCTGTACGTAA
GATGAAGGAGGTTGATTGGC

Downstream 100 bases:

>100_bases
TTTAAATAAACTTTATGACTATAAACGAGCATTCGGATTCTTTAGCCACCAACTCGTTTATCCAGAAAAATTAAATTTTC
ATCCTAGAGAATTCGACGGG

Product: respiratory nitrate reductase beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKIKAQVAMVMNLDKCIGCHTCSITCKSTWTNRPGAEYMWFNNVETKPGIGYPKRWEDQEVYKGGWQLNSKGKLELKSGS
KLSKIALGKIFYNPDMPEMKDYYEPWTYNYANLTNAKEQKHSPVATAESLVSGKKMDLQWGPNWEDDLAGAHITGPTDPN
IQKIEEEIKFNFEQSFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRVEAGLPTVCSETCTGRMRYLGVLLYDADRVLEAATVKDPQDLYQAQLDLFLDPHDPEVIAQAERDGIAQD
WIEAAQNSPVYKLAIEYKLAFPLHPEYRTLPMVWYCPPLSPIMNYFEGKDSIKNPDMIFPAIEEMRLPIQYLANMLTAGD
AQTVKEALQRMAMMRSYMRAVSSGKDFDASRLDRVGMTAHQVKQMYRLLAIAKHEDRFVIPTSHKEGYMNTYSAQGGEGY
TSDNFGADCDGCGPVPAGKSGKEVYEDNFYGGIWRD

Sequences:

>Translated_516_residues
MKIKAQVAMVMNLDKCIGCHTCSITCKSTWTNRPGAEYMWFNNVETKPGIGYPKRWEDQEVYKGGWQLNSKGKLELKSGS
KLSKIALGKIFYNPDMPEMKDYYEPWTYNYANLTNAKEQKHSPVATAESLVSGKKMDLQWGPNWEDDLAGAHITGPTDPN
IQKIEEEIKFNFEQSFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRVEAGLPTVCSETCTGRMRYLGVLLYDADRVLEAATVKDPQDLYQAQLDLFLDPHDPEVIAQAERDGIAQD
WIEAAQNSPVYKLAIEYKLAFPLHPEYRTLPMVWYCPPLSPIMNYFEGKDSIKNPDMIFPAIEEMRLPIQYLANMLTAGD
AQTVKEALQRMAMMRSYMRAVSSGKDFDASRLDRVGMTAHQVKQMYRLLAIAKHEDRFVIPTSHKEGYMNTYSAQGGEGY
TSDNFGADCDGCGPVPAGKSGKEVYEDNFYGGIWRD
>Mature_516_residues
MKIKAQVAMVMNLDKCIGCHTCSITCKSTWTNRPGAEYMWFNNVETKPGIGYPKRWEDQEVYKGGWQLNSKGKLELKSGS
KLSKIALGKIFYNPDMPEMKDYYEPWTYNYANLTNAKEQKHSPVATAESLVSGKKMDLQWGPNWEDDLAGAHITGPTDPN
IQKIEEEIKFNFEQSFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
AEKCTFCFPRVEAGLPTVCSETCTGRMRYLGVLLYDADRVLEAATVKDPQDLYQAQLDLFLDPHDPEVIAQAERDGIAQD
WIEAAQNSPVYKLAIEYKLAFPLHPEYRTLPMVWYCPPLSPIMNYFEGKDSIKNPDMIFPAIEEMRLPIQYLANMLTAGD
AQTVKEALQRMAMMRSYMRAVSSGKDFDASRLDRVGMTAHQVKQMYRLLAIAKHEDRFVIPTSHKEGYMNTYSAQGGEGY
TSDNFGADCDGCGPVPAGKSGKEVYEDNFYGGIWRD

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=492, Percent_Identity=59.1463414634146, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=493, Percent_Identity=58.6206896551724, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=42.5742574257426, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=42.5742574257426, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=40.6976744186046, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=37.1134020618557, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=36.7816091954023, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=35.632183908046, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58640; Mature: 58640

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
8.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKAQVAMVMNLDKCIGCHTCSITCKSTWTNRPGAEYMWFNNVETKPGIGYPKRWEDQE
CCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCH
VYKGGWQLNSKGKLELKSGSKLSKIALGKIFYNPDMPEMKDYYEPWTYNYANLTNAKEQK
HHCCCEEECCCCCEEECCCCCCHHEEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCHHHH
HSPVATAESLVSGKKMDLQWGPNWEDDLAGAHITGPTDPNIQKIEEEIKFNFEQSFMMYL
CCCHHHHHHHHCCCCEEECCCCCCCHHCCCCEECCCCCCCHHHHHHHHHCCHHHHHHHHH
PRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
HHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHCCHHHHHCCCCCEEEEEEECCCC
AEKCTFCFPRVEAGLPTVCSETCTGRMRYLGVLLYDADRVLEAATVKDPQDLYQAQLDLF
CCEEEEECCHHHCCCCHHHHHHHCCHHHHHEEEEECHHHHHHHHHCCCHHHHHHHHHHEE
LDPHDPEVIAQAERDGIAQDWIEAAQNSPVYKLAIEYKLAFPLHPEYRTLPMVWYCPPLS
ECCCCCHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCHH
PIMNYFEGKDSIKNPDMIFPAIEEMRLPIQYLANMLTAGDAQTVKEALQRMAMMRSYMRA
HHHHHHCCCCCCCCCCEEHHCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VSSGKDFDASRLDRVGMTAHQVKQMYRLLAIAKHEDRFVIPTSHKEGYMNTYSAQGGEGY
HHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC
TSDNFGADCDGCGPVPAGKSGKEVYEDNFYGGIWRD
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIKAQVAMVMNLDKCIGCHTCSITCKSTWTNRPGAEYMWFNNVETKPGIGYPKRWEDQE
CCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCH
VYKGGWQLNSKGKLELKSGSKLSKIALGKIFYNPDMPEMKDYYEPWTYNYANLTNAKEQK
HHCCCEEECCCCCEEECCCCCCHHEEEEEEEECCCCCHHHHHCCCCCCCCCCCCCCHHHH
HSPVATAESLVSGKKMDLQWGPNWEDDLAGAHITGPTDPNIQKIEEEIKFNFEQSFMMYL
CCCHHHHHHHHCCCCEEECCCCCCCHHCCCCEECCCCCCCHHHHHHHHHCCHHHHHHHHH
PRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNK
HHHHHHHCCCHHHHCCCCCCCEEECCCCEEEECCHHHCCHHHHHCCCCCEEEEEEECCCC
AEKCTFCFPRVEAGLPTVCSETCTGRMRYLGVLLYDADRVLEAATVKDPQDLYQAQLDLF
CCEEEEECCHHHCCCCHHHHHHHCCHHHHHEEEEECHHHHHHHHHCCCHHHHHHHHHHEE
LDPHDPEVIAQAERDGIAQDWIEAAQNSPVYKLAIEYKLAFPLHPEYRTLPMVWYCPPLS
ECCCCCHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCHH
PIMNYFEGKDSIKNPDMIFPAIEEMRLPIQYLANMLTAGDAQTVKEALQRMAMMRSYMRA
HHHHHHCCCCCCCCCCEEHHCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VSSGKDFDASRLDRVGMTAHQVKQMYRLLAIAKHEDRFVIPTSHKEGYMNTYSAQGGEGY
HHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCC
TSDNFGADCDGCGPVPAGKSGKEVYEDNFYGGIWRD
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]