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The map label for this gene is femA [H]

Identifier: 222150378

GI number: 222150378

Start: 132267

End: 133505

Strand: Direct

Name: femA [H]

Synonym: MCCL_0128

Alternate gene names: 222150378

Gene position: 132267-133505 (Clockwise)

Preceding gene: 222150377

Following gene: 222150381

Centisome position: 6.29

GC content: 32.61

Gene sequence:

>1239_bases
ATGAAGTTTTGCGAGTTACAACCAGATGAATTTGAATCCTTTGTGAATGAGCATTTCAGTCATTACACACAATCAAGAAT
TCATTACGATTATAAAGTTGAGCATAATGAACCTGTACATATTGTTGGGGTTAAAGAGGGAGATGAAGTACTTGCTGCAT
GTCTATTAACGAGTGCACGCGCTTTCAAGTACTTTAACTATTTCTACTCGCATCGTGGTCCTGTGATGGATTATTCAAAT
AAGACGCTTGTTGATTTATTCTATACAGAGCTAAGAGGATATTTAAAGTCACATAATGGTTTATTTGCATTGATAGATCC
ACATATTATTATGAACTATAGAGATCACGATGGTAATATTACGGAGTCAAATGATGTGGAAGGCTTTATTGAACAGATGG
AAGGTTTAGGGTACCATCACCGTGGATTCCCAGTTGGGTATTCTGATGATTCACAAGCCAGGTTCTTATCAGTACTTAAT
CTTGAAGGTAAATCTGAAGATGAATTATTAAAAGAGATGGAATATAAAACGAGACGTAATATTAAGAAAACCTATGAGAT
GGGTGTTCGTTTAAGAGATTTATCGATTGAAGAAACAGATAAATTCTTTGAATTATTCCAGATGGCTGAAGATAAGCATG
GTTTCTCGTTCAGAGACTTTGATTACTTTGTCAAGATGCAAAAGATATACAAAGATCAATGTAGACTTGTATTAAGTTAT
ATTGATTTAGATGAACATCTTGATATTCTGCGTAAAGATTTAAAACGAAAAGAAGACGAATATAATAAAGAGAAACTTAA
GCTGGATGAAAATCCTGGTTTCAGAAAATTACGTAATAAAGTTGATCAGCTTGCCAAAGCAGTTGATAAAGAGAAGAAGA
AAGTTACAGAAATTGAAGCTTTGAAGCAGACAGACGGCAGAATTCTTCATTTAGCAAGTGCATTGTATATTCATAATGAA
CATGAATTATATTATTTATCAAGTGGTTCTAATCCTAAGTACAATCAATTTATGGGGCCCTATAATATGATGTTTGAAAT
GATTAAGTATGCTAAGAGCCTGAATTTGAAAACTTATAATTTTTATGGTGTAACGGGTGTATTCACTGAAGATGCTGAAG
ATTATGGTGTACTTAAATTTAAGAAAGGCTTCAATGCACATATTGAAGAATATATTGGAGACTTTATTTTACCACTTAGC
CCGTTTTATAAATTATATGAATTGAGACAGAAATTCTAA

Upstream 100 bases:

>100_bases
TAAATAAATGATTCCACAACAGAGTATATTGTATCTCTGTTGTTTTCATGTGGTGAATGTTATATTAATAACAAATAGTG
AAATAGAGAGGGAGTACAAG

Downstream 100 bases:

>100_bases
TTAATAAGCCGATACTTCTTAGAAGTATCGGCTTATTAATCTATTATATTACTCTACGATTTCTAATGCGATTTCCATCA
TATTAGTGAATGATGTTTGA

Product: lysostaphin immunity factor FmhA

Products: NA

Alternate protein names: Factor essential for expression of methicillin resistance A [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKFCELQPDEFESFVNEHFSHYTQSRIHYDYKVEHNEPVHIVGVKEGDEVLAACLLTSARAFKYFNYFYSHRGPVMDYSN
KTLVDLFYTELRGYLKSHNGLFALIDPHIIMNYRDHDGNITESNDVEGFIEQMEGLGYHHRGFPVGYSDDSQARFLSVLN
LEGKSEDELLKEMEYKTRRNIKKTYEMGVRLRDLSIEETDKFFELFQMAEDKHGFSFRDFDYFVKMQKIYKDQCRLVLSY
IDLDEHLDILRKDLKRKEDEYNKEKLKLDENPGFRKLRNKVDQLAKAVDKEKKKVTEIEALKQTDGRILHLASALYIHNE
HELYYLSSGSNPKYNQFMGPYNMMFEMIKYAKSLNLKTYNFYGVTGVFTEDAEDYGVLKFKKGFNAHIEEYIGDFILPLS
PFYKLYELRQKF

Sequences:

>Translated_412_residues
MKFCELQPDEFESFVNEHFSHYTQSRIHYDYKVEHNEPVHIVGVKEGDEVLAACLLTSARAFKYFNYFYSHRGPVMDYSN
KTLVDLFYTELRGYLKSHNGLFALIDPHIIMNYRDHDGNITESNDVEGFIEQMEGLGYHHRGFPVGYSDDSQARFLSVLN
LEGKSEDELLKEMEYKTRRNIKKTYEMGVRLRDLSIEETDKFFELFQMAEDKHGFSFRDFDYFVKMQKIYKDQCRLVLSY
IDLDEHLDILRKDLKRKEDEYNKEKLKLDENPGFRKLRNKVDQLAKAVDKEKKKVTEIEALKQTDGRILHLASALYIHNE
HELYYLSSGSNPKYNQFMGPYNMMFEMIKYAKSLNLKTYNFYGVTGVFTEDAEDYGVLKFKKGFNAHIEEYIGDFILPLS
PFYKLYELRQKF
>Mature_412_residues
MKFCELQPDEFESFVNEHFSHYTQSRIHYDYKVEHNEPVHIVGVKEGDEVLAACLLTSARAFKYFNYFYSHRGPVMDYSN
KTLVDLFYTELRGYLKSHNGLFALIDPHIIMNYRDHDGNITESNDVEGFIEQMEGLGYHHRGFPVGYSDDSQARFLSVLN
LEGKSEDELLKEMEYKTRRNIKKTYEMGVRLRDLSIEETDKFFELFQMAEDKHGFSFRDFDYFVKMQKIYKDQCRLVLSY
IDLDEHLDILRKDLKRKEDEYNKEKLKLDENPGFRKLRNKVDQLAKAVDKEKKKVTEIEALKQTDGRILHLASALYIHNE
HELYYLSSGSNPKYNQFMGPYNMMFEMIKYAKSLNLKTYNFYGVTGVFTEDAEDYGVLKFKKGFNAHIEEYIGDFILPLS
PFYKLYELRQKF

Specific function: Catalyzes the formation of the pentaglycine interpeptide bridge, which is characteristic of the S.aureus peptidoglycan. Adds glycines 2 and 3 of the pentaglycine bridge, using glycyl- tRNA(Gly) as donor. Involved in resistance to methicillin [H]

COG id: COG2348

COG function: function code V; Uncharacterized protein involved in methicillin resistance

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the femABX family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR003447
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02388 FemAB [H]

EC number: NA

Molecular weight: Translated: 48921; Mature: 48921

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFCELQPDEFESFVNEHFSHYTQSRIHYDYKVEHNEPVHIVGVKEGDEVLAACLLTSAR
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHH
AFKYFNYFYSHRGPVMDYSNKTLVDLFYTELRGYLKSHNGLFALIDPHIIMNYRDHDGNI
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCC
TESNDVEGFIEQMEGLGYHHRGFPVGYSDDSQARFLSVLNLEGKSEDELLKEMEYKTRRN
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IKKTYEMGVRLRDLSIEETDKFFELFQMAEDKHGFSFRDFDYFVKMQKIYKDQCRLVLSY
HHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IDLDEHLDILRKDLKRKEDEYNKEKLKLDENPGFRKLRNKVDQLAKAVDKEKKKVTEIEA
HCHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQTDGRILHLASALYIHNEHELYYLSSGSNPKYNQFMGPYNMMFEMIKYAKSLNLKTYN
HHHCCCCEEEEEHHHEEECCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEEE
FYGVTGVFTEDAEDYGVLKFKKGFNAHIEEYIGDFILPLSPFYKLYELRQKF
EEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFCELQPDEFESFVNEHFSHYTQSRIHYDYKVEHNEPVHIVGVKEGDEVLAACLLTSAR
CCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHH
AFKYFNYFYSHRGPVMDYSNKTLVDLFYTELRGYLKSHNGLFALIDPHIIMNYRDHDGNI
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCC
TESNDVEGFIEQMEGLGYHHRGFPVGYSDDSQARFLSVLNLEGKSEDELLKEMEYKTRRN
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
IKKTYEMGVRLRDLSIEETDKFFELFQMAEDKHGFSFRDFDYFVKMQKIYKDQCRLVLSY
HHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IDLDEHLDILRKDLKRKEDEYNKEKLKLDENPGFRKLRNKVDQLAKAVDKEKKKVTEIEA
HCHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKQTDGRILHLASALYIHNEHELYYLSSGSNPKYNQFMGPYNMMFEMIKYAKSLNLKTYN
HHHCCCCEEEEEHHHEEECCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCEEEE
FYGVTGVFTEDAEDYGVLKFKKGFNAHIEEYIGDFILPLSPFYKLYELRQKF
EEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA