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The map label for this gene is brnQ [H]

Identifier: 222150300

GI number: 222150300

Start: 52478

End: 53848

Strand: Direct

Name: brnQ [H]

Synonym: MCCL_0050

Alternate gene names: 222150300

Gene position: 52478-53848 (Clockwise)

Preceding gene: 222150299

Following gene: 222150302

Centisome position: 2.5

GC content: 34.35

Gene sequence:

>1371_bases
ATGATTATTAACGCTAAAACGAAATTAACATTTAAAGAGAATATGTTTATCGGATCAATGCTGTTTGGACTATTCTTCGG
GGCTGGAAATTTAATTTTCCCGATTCATTTCGGTCAAACAGCAGGTAGCAATGTATGGCTTACAAACTTAGGATTTCTCA
TTACTGCGATTGGTTTACCATTCTTAGGTATAATCGCCATTGGTATATCTAAAACAAATGGTGTATTTGATATTTCAAAT
CGAGTCAACCGTTCATATGCATACATATTCACGATTCTTCTATATTTAGTTATTGGTCCATTATTTGCGTTGCCAAGGCT
TGCAACAACGTCGTTTGAAATTGGTTTTTCTTCATACGTGACAGAACAAAATGGAAAATTGTTTTTATTTATTTTTAGTT
TATTATTCTTTTTAGTTGCATGGATGTTCTCACGTAAACCTTCTAAAATATTGGATTATATCGGTAAATTCTTAAATCCT
GTCTTTTTATTTCTTTTAGGGGTTTTAATGTTACTTGCATTTATTAAACCGATGGGAAGCATTGGAAGCGCACCTATACA
GGCCGACTATCAATCCAACGCCGTACTTAAAGGTTTTATCGATGGCTACAACACATTAGACGCGCTCGCTTCACTTGCAT
TTGGTATTATTATTGTAACGACAATTAAGAAATTAGGGATTACAAACCCTGCACATATTGCGAAAGAAACGGTGAAATCT
GGATTTATCAGTATTATTCTTATGGGATTAATCTATACATTACTTGCCCTTATGGGAACGATGAGTTTAGGTCAATTTAA
AGTGAGTGAGAACGGTGGGATTGCATTAGCTCAAATTGCACACTACTATTTAGGTGATTACGGTATTATTCTATTATCTT
TAATCATTATTGTAGCTTGTTTAAAGACAGCAATTGGCTTAATTACAGCATTCTCAGAAACATTTACAGAATTATTCCCG
AAACGTAACTATTTAATGTTTGCAACAGTTGTGAGTATCGTTTCATTCGTCATTGCTAATGTGGGATTAACAAAGATAAT
TACATATTCTATACCAGTATTAATGTTTTTGTATCCATTAGCGATCACGCTTATTTTACTGACATTATTCAGTAAGTATT
TTAATCATTCACGTACAGTTTATCAATTTACAACGTACTTTACGATGATTGCAGCGTTTGTGGATGGTTTAAAAGCAAGT
CCGGAGTTTATTGCGAATACAGCATTCGTGAAAGGGGTCGTGAACTTTGGAGATCACTATTTGCCCCTCTCCTCGATTGG
GATGGGATGGGTATTGCCGGCAGTAATCGGATTTGTGATCGGATATATTATTTATTTAGCAAGAGAGAAGAATACAGTTA
CAGAAGCTTAA

Upstream 100 bases:

>100_bases
AATAAAGTAAATATAATTTTTTAATTAATCAGAAAATTCATATACTCAAACATACTTTAAAGTGATATAATTAAAAACTA
AATTCACAATGAAGGAGTGT

Downstream 100 bases:

>100_bases
TATTTCACGTAAAACACCTTACAGGAAATCTGTAAGGTGTTTTATCGACTTCGCTTTTAAATTATTAATCACCAATAACA
GTAAGTTCAACATCAATAGA

Product: branched-chain amino acid transporter carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MIINAKTKLTFKENMFIGSMLFGLFFGAGNLIFPIHFGQTAGSNVWLTNLGFLITAIGLPFLGIIAIGISKTNGVFDISN
RVNRSYAYIFTILLYLVIGPLFALPRLATTSFEIGFSSYVTEQNGKLFLFIFSLLFFLVAWMFSRKPSKILDYIGKFLNP
VFLFLLGVLMLLAFIKPMGSIGSAPIQADYQSNAVLKGFIDGYNTLDALASLAFGIIIVTTIKKLGITNPAHIAKETVKS
GFISIILMGLIYTLLALMGTMSLGQFKVSENGGIALAQIAHYYLGDYGIILLSLIIIVACLKTAIGLITAFSETFTELFP
KRNYLMFATVVSIVSFVIANVGLTKIITYSIPVLMFLYPLAITLILLTLFSKYFNHSRTVYQFTTYFTMIAAFVDGLKAS
PEFIANTAFVKGVVNFGDHYLPLSSIGMGWVLPAVIGFVIGYIIYLAREKNTVTEA

Sequences:

>Translated_456_residues
MIINAKTKLTFKENMFIGSMLFGLFFGAGNLIFPIHFGQTAGSNVWLTNLGFLITAIGLPFLGIIAIGISKTNGVFDISN
RVNRSYAYIFTILLYLVIGPLFALPRLATTSFEIGFSSYVTEQNGKLFLFIFSLLFFLVAWMFSRKPSKILDYIGKFLNP
VFLFLLGVLMLLAFIKPMGSIGSAPIQADYQSNAVLKGFIDGYNTLDALASLAFGIIIVTTIKKLGITNPAHIAKETVKS
GFISIILMGLIYTLLALMGTMSLGQFKVSENGGIALAQIAHYYLGDYGIILLSLIIIVACLKTAIGLITAFSETFTELFP
KRNYLMFATVVSIVSFVIANVGLTKIITYSIPVLMFLYPLAITLILLTLFSKYFNHSRTVYQFTTYFTMIAAFVDGLKAS
PEFIANTAFVKGVVNFGDHYLPLSSIGMGWVLPAVIGFVIGYIIYLAREKNTVTEA
>Mature_456_residues
MIINAKTKLTFKENMFIGSMLFGLFFGAGNLIFPIHFGQTAGSNVWLTNLGFLITAIGLPFLGIIAIGISKTNGVFDISN
RVNRSYAYIFTILLYLVIGPLFALPRLATTSFEIGFSSYVTEQNGKLFLFIFSLLFFLVAWMFSRKPSKILDYIGKFLNP
VFLFLLGVLMLLAFIKPMGSIGSAPIQADYQSNAVLKGFIDGYNTLDALASLAFGIIIVTTIKKLGITNPAHIAKETVKS
GFISIILMGLIYTLLALMGTMSLGQFKVSENGGIALAQIAHYYLGDYGIILLSLIIIVACLKTAIGLITAFSETFTELFP
KRNYLMFATVVSIVSFVIANVGLTKIITYSIPVLMFLYPLAITLILLTLFSKYFNHSRTVYQFTTYFTMIAAFVDGLKAS
PEFIANTAFVKGVVNFGDHYLPLSSIGMGWVLPAVIGFVIGYIIYLAREKNTVTEA

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=452, Percent_Identity=34.2920353982301, Blast_Score=244, Evalue=6e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 50086; Mature: 50086

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINAKTKLTFKENMFIGSMLFGLFFGAGNLIFPIHFGQTAGSNVWLTNLGFLITAIGLP
CEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHH
FLGIIAIGISKTNGVFDISNRVNRSYAYIFTILLYLVIGPLFALPRLATTSFEIGFSSYV
HHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHH
TEQNGKLFLFIFSLLFFLVAWMFSRKPSKILDYIGKFLNPVFLFLLGVLMLLAFIKPMGS
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IGSAPIQADYQSNAVLKGFIDGYNTLDALASLAFGIIIVTTIKKLGITNPAHIAKETVKS
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GFISIILMGLIYTLLALMGTMSLGQFKVSENGGIALAQIAHYYLGDYGIILLSLIIIVAC
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTAIGLITAFSETFTELFPKRNYLMFATVVSIVSFVIANVGLTKIITYSIPVLMFLYPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AITLILLTLFSKYFNHSRTVYQFTTYFTMIAAFVDGLKASPEFIANTAFVKGVVNFGDHY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LPLSSIGMGWVLPAVIGFVIGYIIYLAREKNTVTEA
CCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIINAKTKLTFKENMFIGSMLFGLFFGAGNLIFPIHFGQTAGSNVWLTNLGFLITAIGLP
CEECCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEHHHHHHHHHHHHHH
FLGIIAIGISKTNGVFDISNRVNRSYAYIFTILLYLVIGPLFALPRLATTSFEIGFSSYV
HHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHH
TEQNGKLFLFIFSLLFFLVAWMFSRKPSKILDYIGKFLNPVFLFLLGVLMLLAFIKPMGS
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IGSAPIQADYQSNAVLKGFIDGYNTLDALASLAFGIIIVTTIKKLGITNPAHIAKETVKS
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
GFISIILMGLIYTLLALMGTMSLGQFKVSENGGIALAQIAHYYLGDYGIILLSLIIIVAC
HHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LKTAIGLITAFSETFTELFPKRNYLMFATVVSIVSFVIANVGLTKIITYSIPVLMFLYPL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AITLILLTLFSKYFNHSRTVYQFTTYFTMIAAFVDGLKASPEFIANTAFVKGVVNFGDHY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LPLSSIGMGWVLPAVIGFVIGYIIYLAREKNTVTEA
CCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8544834 [H]