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The map label for this gene is orfX

Identifier: 222150276

GI number: 222150276

Start: 31891

End: 32370

Strand: Direct

Name: orfX

Synonym: MCCL_0026

Alternate gene names: 222150276

Gene position: 31891-32370 (Clockwise)

Preceding gene: 222150275

Following gene: 222150277

Centisome position: 1.52

GC content: 40.0

Gene sequence:

>480_bases
ATGAAAATAACAATAATAACTGTGGGTAAGTTGAAAGAGAAGTACTGGAAGATGGCTGTAGACGAGTATGTAAAAAGACT
TGGTGCATACTCGAAAATAGAACTCGTTGAGGTAGCTGACGAGAAAGATTCGGATAATATGAGTGAGAAAGATATCGAGA
TTGCGAAGCGGAAAGAAGCGGAACGAATATTGAGTAAGGTGAAAGATGATAGCTTTGTCTACACGTTAGAGATTCTTGGC
AAGCAGCTCGACTCTGTGCAGTTAGCAAAAGAGATGGAGCAAAAGATGAATACCGGTAAAAGTCATCTGACGTTTATTAT
CGGCGGATCAAATGGCCTTCATCAAAATGTTATGGATCGCTCTAACTACGCCCTATCATTTAGCAAGATGACATTTCCGC
ATCAGATGATGAAAGTGATACTGCTGGAGCAGATTTATCGAGGATTTAGGATAATGAAGGGACAGGCCTACCATAAATAA

Upstream 100 bases:

>100_bases
GAATAACCTTTAAAATGTGATTATAATAAGATTGTGAAATGTGAATATGTGATTAACTTGTGGATAAGTAGAATAACTTT
TATAAAAGGAATGAATAAAC

Downstream 100 bases:

>100_bases
AACTAAGTACATTCATTTTGAATGTACTTTAAAAAGTTATTTAAGAATCGCTAACTGCCAAAATTATTTTAAATTAATAA
GACGGAAGAACAACCTATAC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKITIITVGKLKEKYWKMAVDEYVKRLGAYSKIELVEVADEKDSDNMSEKDIEIAKRKEAERILSKVKDDSFVYTLEILG
KQLDSVQLAKEMEQKMNTGKSHLTFIIGGSNGLHQNVMDRSNYALSFSKMTFPHQMMKVILLEQIYRGFRIMKGQAYHK

Sequences:

>Translated_159_residues
MKITIITVGKLKEKYWKMAVDEYVKRLGAYSKIELVEVADEKDSDNMSEKDIEIAKRKEAERILSKVKDDSFVYTLEILG
KQLDSVQLAKEMEQKMNTGKSHLTFIIGGSNGLHQNVMDRSNYALSFSKMTFPHQMMKVILLEQIYRGFRIMKGQAYHK
>Mature_159_residues
MKITIITVGKLKEKYWKMAVDEYVKRLGAYSKIELVEVADEKDSDNMSEKDIEIAKRKEAERILSKVKDDSFVYTLEILG
KQLDSVQLAKEMEQKMNTGKSHLTFIIGGSNGLHQNVMDRSNYALSFSKMTFPHQMMKVILLEQIYRGFRIMKGQAYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=30.188679245283, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_MACCJ (B9E922)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559429.1
- ProteinModelPortal:   B9E922
- GeneID:   7389677
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0026
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18431; Mature: 18431

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITIITVGKLKEKYWKMAVDEYVKRLGAYSKIELVEVADEKDSDNMSEKDIEIAKRKEA
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ERILSKVKDDSFVYTLEILGKQLDSVQLAKEMEQKMNTGKSHLTFIIGGSNGLHQNVMDR
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHC
SNYALSFSKMTFPHQMMKVILLEQIYRGFRIMKGQAYHK
CCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKITIITVGKLKEKYWKMAVDEYVKRLGAYSKIELVEVADEKDSDNMSEKDIEIAKRKEA
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHH
ERILSKVKDDSFVYTLEILGKQLDSVQLAKEMEQKMNTGKSHLTFIIGGSNGLHQNVMDR
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHC
SNYALSFSKMTFPHQMMKVILLEQIYRGFRIMKGQAYHK
CCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA