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The map label for this gene is alsS [H]

Identifier: 222150260

GI number: 222150260

Start: 12692

End: 14359

Strand: Direct

Name: alsS [H]

Synonym: MCCL_0010

Alternate gene names: 222150260

Gene position: 12692-14359 (Clockwise)

Preceding gene: 222150259

Following gene: 222150261

Centisome position: 0.6

GC content: 37.53

Gene sequence:

>1668_bases
ATGAAAAAATCTGGTGGAGATTTATTTGTTGATACTCTAATTAATCATGGAGTAACTCATGTTTTTGGTATTCCAGGAGC
TAAAATTGATAAAGCATTTGATGTACTCGAAGACAGAGGACCGAAAGTTATTGTGACACGCCATGAACAAAACGCGGCTA
TGATTGCACAAGGTATAGGGCGTATTACTGGTAAACCTGGTGTTGTTCTTGTGACAAGCGGGCCAGGAGTAACGAACTTA
ACTACTGGACTGATTACTGCAAATTCAGAAAGTGATCCGATTGTAGCCATTGGTGGTAACGTAAGACGCGAAGATAGTTT
AAAACGTACACATCAATCACTTGATAATGCTGGATTAATGAAGCACGTTACAAAATATTCTGTAGAAGCTCAAGATGCGA
AAAGTATTTCTGAGGCAGTGACGAATGCATTTCGTGAGAGTGTCGAAGGCCGTAAAGGTGCAGCGTTTGTAAGTATTCCT
CAAGATGTTATTTCTGCTGATAATATAGAGGGGGAAGCTATTAAAGTAAAAGAAGCTCCAGTCCATGGACCTGCACCAGT
TCACGAAATTGATGCATTGGTTGAACGTATTAAATCAGCAAAATTGCCTGTACTTCTTTTAGGAATGAGAGCTTCTTCTG
AAGCATGTACAGCATCTATTCGCGAATTACTCTCAAAAACGCCGATGCCAGTTGTAGAAACGTTCCAGGGCTCTGGTATT
CTATCTCGTGAATTAGAACATAATTTCTATGGGAGAATAGGTTTATTCCGTAATCAACCTGGAGATGAATTATTAAAACA
AGCTGACTTAGTCATCACCGTTGGATATGACCCTATTGAATATGATCCATTTATCTGGAATGCAGAAAATGAAAAAACAA
TCATTCACATTGATGAAGTACAAAGTGATATCGATAACAACTATGCACCAGTTACTGAATTGATTGGTAATACATCTTTA
ACAATTCAACGTTTGAATGAAAAACTTCAAAAAGATGATGTCGAATTAGATGACACAACTAAAGAAATATTAGAAAAACT
TCAAAAGTTAATTGAAAAAAAATATGAGCTTCCCGAAAATTATAAAGACAGTGAAAAAGTTAATCCAAGACATATTGTTA
AAGCAGTACGCGATATAATTGATGATGAAACAACTGTTACTGTAGATGTAGGTTCACTCTATATCTATATGGCACGTTAT
TTCAGAAGTTATGCGCCAAGACATCTGCTCTTTTCAAATGGAATGCAGACGTTAGGTGTTGCTCTACCTTGGGCTATTGC
CGCATCTATTGCACGTCCTGGTAAAAAAGTAGTTTCGATGGCGGGAGATGGTGGATTCTTATTCTCAGGACAAGAATTAA
TAACAGCAGTTGAAATGAAAGCCCCGATCGTACAATTAATCTGGAACGATGGCTACTATGATATGGTTAAATTCCAGCAA
GAAGGTAAATATGGCAAAGGTGCAGCCGTTAGATTGCACCCAGTGGATTATGTGAAATATGCTGAATCTATGGGTGCTAA
AGGGTATCGTGTGAACCATGCTAGTGAATTAGAAGAAATTCTAGAAAAAGCAATGCAAGAAGAAGGACCAGTTGTAGTAG
ATATTCCTGTAGATTACAGCAATAACTCTAAATTAATGGCAGAGCTTTTGCCTGATAGCACAAACTAA

Upstream 100 bases:

>100_bases
AAAATATATTTCTCAGTTTAATTATTTGCCATTGAAAATATTTAGTAATATTATAAAAATGTAATAAAAAAGCACAAAAT
TATATTTAGGAGAGATAATG

Downstream 100 bases:

>100_bases
GGAGGAACTTATGAATCATTTATATCAATATTCAACCATGGGTGCGTTAGTTGGTGGTTTGTTCGAAGGCACGTTTAAGA
TGTCAGAAGTACTGAAATCA

Product: acetolactate synthase

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKKSGGDLFVDTLINHGVTHVFGIPGAKIDKAFDVLEDRGPKVIVTRHEQNAAMIAQGIGRITGKPGVVLVTSGPGVTNL
TTGLITANSESDPIVAIGGNVRREDSLKRTHQSLDNAGLMKHVTKYSVEAQDAKSISEAVTNAFRESVEGRKGAAFVSIP
QDVISADNIEGEAIKVKEAPVHGPAPVHEIDALVERIKSAKLPVLLLGMRASSEACTASIRELLSKTPMPVVETFQGSGI
LSRELEHNFYGRIGLFRNQPGDELLKQADLVITVGYDPIEYDPFIWNAENEKTIIHIDEVQSDIDNNYAPVTELIGNTSL
TIQRLNEKLQKDDVELDDTTKEILEKLQKLIEKKYELPENYKDSEKVNPRHIVKAVRDIIDDETTVTVDVGSLYIYMARY
FRSYAPRHLLFSNGMQTLGVALPWAIAASIARPGKKVVSMAGDGGFLFSGQELITAVEMKAPIVQLIWNDGYYDMVKFQQ
EGKYGKGAAVRLHPVDYVKYAESMGAKGYRVNHASELEEILEKAMQEEGPVVVDIPVDYSNNSKLMAELLPDSTN

Sequences:

>Translated_555_residues
MKKSGGDLFVDTLINHGVTHVFGIPGAKIDKAFDVLEDRGPKVIVTRHEQNAAMIAQGIGRITGKPGVVLVTSGPGVTNL
TTGLITANSESDPIVAIGGNVRREDSLKRTHQSLDNAGLMKHVTKYSVEAQDAKSISEAVTNAFRESVEGRKGAAFVSIP
QDVISADNIEGEAIKVKEAPVHGPAPVHEIDALVERIKSAKLPVLLLGMRASSEACTASIRELLSKTPMPVVETFQGSGI
LSRELEHNFYGRIGLFRNQPGDELLKQADLVITVGYDPIEYDPFIWNAENEKTIIHIDEVQSDIDNNYAPVTELIGNTSL
TIQRLNEKLQKDDVELDDTTKEILEKLQKLIEKKYELPENYKDSEKVNPRHIVKAVRDIIDDETTVTVDVGSLYIYMARY
FRSYAPRHLLFSNGMQTLGVALPWAIAASIARPGKKVVSMAGDGGFLFSGQELITAVEMKAPIVQLIWNDGYYDMVKFQQ
EGKYGKGAAVRLHPVDYVKYAESMGAKGYRVNHASELEEILEKAMQEEGPVVVDIPVDYSNNSKLMAELLPDSTN
>Mature_555_residues
MKKSGGDLFVDTLINHGVTHVFGIPGAKIDKAFDVLEDRGPKVIVTRHEQNAAMIAQGIGRITGKPGVVLVTSGPGVTNL
TTGLITANSESDPIVAIGGNVRREDSLKRTHQSLDNAGLMKHVTKYSVEAQDAKSISEAVTNAFRESVEGRKGAAFVSIP
QDVISADNIEGEAIKVKEAPVHGPAPVHEIDALVERIKSAKLPVLLLGMRASSEACTASIRELLSKTPMPVVETFQGSGI
LSRELEHNFYGRIGLFRNQPGDELLKQADLVITVGYDPIEYDPFIWNAENEKTIIHIDEVQSDIDNNYAPVTELIGNTSL
TIQRLNEKLQKDDVELDDTTKEILEKLQKLIEKKYELPENYKDSEKVNPRHIVKAVRDIIDDETTVTVDVGSLYIYMARY
FRSYAPRHLLFSNGMQTLGVALPWAIAASIARPGKKVVSMAGDGGFLFSGQELITAVEMKAPIVQLIWNDGYYDMVKFQQ
EGKYGKGAAVRLHPVDYVKYAESMGAKGYRVNHASELEEILEKAMQEEGPVVVDIPVDYSNNSKLMAELLPDSTN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=573, Percent_Identity=26.8760907504363, Blast_Score=183, Evalue=5e-46,
Organism=Homo sapiens, GI21361361, Length=592, Percent_Identity=25.1689189189189, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI87081685, Length=552, Percent_Identity=28.2608695652174, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=27.1428571428571, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=25.9459459459459, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1787096, Length=554, Percent_Identity=25.2707581227437, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788716, Length=552, Percent_Identity=24.8188405797101, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=565, Percent_Identity=26.0176991150442, Blast_Score=151, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=26.0176991150442, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=581, Percent_Identity=24.2685025817556, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=569, Percent_Identity=27.2407732864675, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=544, Percent_Identity=27.5735294117647, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19922626, Length=567, Percent_Identity=24.5149911816578, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60995; Mature: 60995

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSGGDLFVDTLINHGVTHVFGIPGAKIDKAFDVLEDRGPKVIVTRHEQNAAMIAQGIG
CCCCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RITGKPGVVLVTSGPGVTNLTTGLITANSESDPIVAIGGNVRREDSLKRTHQSLDNAGLM
HCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHH
KHVTKYSVEAQDAKSISEAVTNAFRESVEGRKGAAFVSIPQDVISADNIEGEAIKVKEAP
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCEEEEEECC
VHGPAPVHEIDALVERIKSAKLPVLLLGMRASSEACTASIRELLSKTPMPVVETFQGSGI
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCC
LSRELEHNFYGRIGLFRNQPGDELLKQADLVITVGYDPIEYDPFIWNAENEKTIIHIDEV
CHHHHHCCCCCCEEECCCCCHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCEEEEEHHH
QSDIDNNYAPVTELIGNTSLTIQRLNEKLQKDDVELDDTTKEILEKLQKLIEKKYELPEN
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
YKDSEKVNPRHIVKAVRDIIDDETTVTVDVGSLYIYMARYFRSYAPRHLLFSNGMQTLGV
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHH
ALPWAIAASIARPGKKVVSMAGDGGFLFSGQELITAVEMKAPIVQLIWNDGYYDMVKFQQ
HHHHHHHHHHHCCCHHHEEECCCCCEEECCHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
EGKYGKGAAVRLHPVDYVKYAESMGAKGYRVNHASELEEILEKAMQEEGPVVVDIPVDYS
CCCCCCCCEEEECCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEECCCC
NNSKLMAELLPDSTN
CCCHHHHHHCCCCCC
>Mature Secondary Structure
MKKSGGDLFVDTLINHGVTHVFGIPGAKIDKAFDVLEDRGPKVIVTRHEQNAAMIAQGIG
CCCCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
RITGKPGVVLVTSGPGVTNLTTGLITANSESDPIVAIGGNVRREDSLKRTHQSLDNAGLM
HCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCHHH
KHVTKYSVEAQDAKSISEAVTNAFRESVEGRKGAAFVSIPQDVISADNIEGEAIKVKEAP
HHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCEEEEEECC
VHGPAPVHEIDALVERIKSAKLPVLLLGMRASSEACTASIRELLSKTPMPVVETFQGSGI
CCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCC
LSRELEHNFYGRIGLFRNQPGDELLKQADLVITVGYDPIEYDPFIWNAENEKTIIHIDEV
CHHHHHCCCCCCEEECCCCCHHHHHHHCCEEEEECCCCCCCCCEEECCCCCCEEEEEHHH
QSDIDNNYAPVTELIGNTSLTIQRLNEKLQKDDVELDDTTKEILEKLQKLIEKKYELPEN
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
YKDSEKVNPRHIVKAVRDIIDDETTVTVDVGSLYIYMARYFRSYAPRHLLFSNGMQTLGV
CCCCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHH
ALPWAIAASIARPGKKVVSMAGDGGFLFSGQELITAVEMKAPIVQLIWNDGYYDMVKFQQ
HHHHHHHHHHHCCCHHHEEECCCCCEEECCHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
EGKYGKGAAVRLHPVDYVKYAESMGAKGYRVNHASELEEILEKAMQEEGPVVVDIPVDYS
CCCCCCCCEEEECCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCCCEEEEEECCCC
NNSKLMAELLPDSTN
CCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]