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The map label for this gene is mii [H]

Identifier: 222111272

GI number: 222111272

Start: 2234482

End: 2235684

Strand: Direct

Name: mii [H]

Synonym: Dtpsy_2081

Alternate gene names: 222111272

Gene position: 2234482-2235684 (Clockwise)

Preceding gene: 222111271

Following gene: 222111273

Centisome position: 58.86

GC content: 71.82

Gene sequence:

>1203_bases
ATGGCACCACAGATCAGGATCCCCGCCACCTACCTGCGCGGCGGCACCAGCAAGGGCGTGTTCTTTCGCCTGCAGGATTT
GCCCGCGGCGGCGCAGCAGCCCGGCGCAGCGCGCGACGCGCTGCTACTGCGCGTGATCGGCAGCCCCGACCCCTACGGCA
AACAGATCGACGGCATGGGCGGCGCCACCTCGTCCACGTCCAAGGCGGTGATCGTCTCGCCCTCCACCCGGCCCGGGCAC
GACGTGGACTATCTGTTCGGCCAGGTCGCCATCGACAGCCCCTTCGTGGACTGGAGCGGCAACTGCGGCAACCTGTCGGC
CGCCGTGGGGCCGTTCGCCATCGCCAGCGGCCTGGTCGCGCCCGGGCGCATTCCGCACGACGGCGTGTGCACCGTACGCA
TCTGGCAGGCCAACATCGGCAAGACCATCGTCGCCCACGTACCCGTCGCAGGTGGCCAGGTGCAGGAGACGGGCGACTTT
GCGCTGGACGGCGTGGCCTTCCCCGCCGCCGAGGTGTTGCTCGAATTCCTTGACCCGGCCGATGGCGGTGAAGGAGGCGA
AGGCGGCGGCAGTGCCCTGTTCCCCACCGGCCAGCTGGTCGATACGCTGGACGTGCCAGGCGTGGGCCGCTTGCAGGCCA
CGCTCATCAACGCGGGCATCCCGACCATCTTCCTGAACGCCAGGGACGTCGGCTGCACCGGCACCGAGCTGCAGGACGCG
ATCAATGGCGATGCCGCGGCCCTGGCCCGCTTCGAGGCCATCCGCGCCTGGGGCGCAGTCAAGATGGGCCTGATCCACGA
CGTGGCAGAGGCCGCCACGCGCCAGCACACGCCCAAACTCGCCTTCGTCGCGCCGCCGGCGGACTACGTCGCCTCCAGCG
GCACGGCAGTGCGCGCGGCCGACATCGACCTGCTGGTGCGCGCACTGTCCATGGGCAAGCTGCACCACGCCATGATGGGC
ACGGCGGCCGTGGCCATCGGCGCCGCTGCCGCCGTCCCCGGCACGCTGGTCCACCAGGCGGCCGGCGGGGGCCCGCGCAA
CGCCGTGCCGGGATCGGTGCGGTTCGGCCACCCCTCGGGCACGCTGCGCGTGGGGGCCGAGGCCGCGCTGGTGAACGGCC
AGTGGCGCGTCACCAAGGCCCTCATGAGCCGCAGCGCCCGCACCCTGATGGAAGGCTGGGTGAGAGTGCCGCCAACCCCG
TGA

Upstream 100 bases:

>100_bases
GCGGGGGGCGTGCTGCAGCGCTTCGCCCAGGACTTTCTGGCCCAATCAGCTTCCAGCGCCCGTCTGACAAGCGCAACCAG
CTATTGAAACAAGAGCAAAC

Downstream 100 bases:

>100_bases
GCAACTTCGTCATATCCCCCTTCTCCCACGCTTCGCGCGGCATGGGGGACGCCGCCAGCGCGGCTGGGCGGCGCGGCCGC
CACAGGCCCTCGCGCCGCGG

Product: acnd-accessory protein prpf

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAPQIRIPATYLRGGTSKGVFFRLQDLPAAAQQPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKAVIVSPSTRPGH
DVDYLFGQVAIDSPFVDWSGNCGNLSAAVGPFAIASGLVAPGRIPHDGVCTVRIWQANIGKTIVAHVPVAGGQVQETGDF
ALDGVAFPAAEVLLEFLDPADGGEGGEGGGSALFPTGQLVDTLDVPGVGRLQATLINAGIPTIFLNARDVGCTGTELQDA
INGDAAALARFEAIRAWGAVKMGLIHDVAEAATRQHTPKLAFVAPPADYVASSGTAVRAADIDLLVRALSMGKLHHAMMG
TAAVAIGAAAAVPGTLVHQAAGGGPRNAVPGSVRFGHPSGTLRVGAEAALVNGQWRVTKALMSRSARTLMEGWVRVPPTP

Sequences:

>Translated_400_residues
MAPQIRIPATYLRGGTSKGVFFRLQDLPAAAQQPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKAVIVSPSTRPGH
DVDYLFGQVAIDSPFVDWSGNCGNLSAAVGPFAIASGLVAPGRIPHDGVCTVRIWQANIGKTIVAHVPVAGGQVQETGDF
ALDGVAFPAAEVLLEFLDPADGGEGGEGGGSALFPTGQLVDTLDVPGVGRLQATLINAGIPTIFLNARDVGCTGTELQDA
INGDAAALARFEAIRAWGAVKMGLIHDVAEAATRQHTPKLAFVAPPADYVASSGTAVRAADIDLLVRALSMGKLHHAMMG
TAAVAIGAAAAVPGTLVHQAAGGGPRNAVPGSVRFGHPSGTLRVGAEAALVNGQWRVTKALMSRSARTLMEGWVRVPPTP
>Mature_399_residues
APQIRIPATYLRGGTSKGVFFRLQDLPAAAQQPGAARDALLLRVIGSPDPYGKQIDGMGGATSSTSKAVIVSPSTRPGHD
VDYLFGQVAIDSPFVDWSGNCGNLSAAVGPFAIASGLVAPGRIPHDGVCTVRIWQANIGKTIVAHVPVAGGQVQETGDFA
LDGVAFPAAEVLLEFLDPADGGEGGEGGGSALFPTGQLVDTLDVPGVGRLQATLINAGIPTIFLNARDVGCTGTELQDAI
NGDAAALARFEAIRAWGAVKMGLIHDVAEAATRQHTPKLAFVAPPADYVASSGTAVRAADIDLLVRALSMGKLHHAMMGT
AAVAIGAAAAVPGTLVHQAAGGGPRNAVPGSVRFGHPSGTLRVGAEAALVNGQWRVTKALMSRSARTLMEGWVRVPPTP

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=398, Percent_Identity=35.1758793969849, Blast_Score=169, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 40811; Mature: 40680

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPQIRIPATYLRGGTSKGVFFRLQDLPAAAQQPGAARDALLLRVIGSPDPYGKQIDGMG
CCCCCCCCHHHHCCCCCCCEEEEEECCCCHHCCCCCCCCEEEEEEECCCCCCCCCCCCCC
GATSSTSKAVIVSPSTRPGHDVDYLFGQVAIDSPFVDWSGNCGNLSAAVGPFAIASGLVA
CCCCCCCCEEEECCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PGRIPHDGVCTVRIWQANIGKTIVAHVPVAGGQVQETGDFALDGVAFPAAEVLLEFLDPA
CCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEECCCCEEECCCHHHHHHHHHHHHCCC
DGGEGGEGGGSALFPTGQLVDTLDVPGVGRLQATLINAGIPTIFLNARDVGCTGTELQDA
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHEEEEEECCCCEEEEECCCCCCCCHHHHHH
INGDAAALARFEAIRAWGAVKMGLIHDVAEAATRQHTPKLAFVAPPADYVASSGTAVRAA
HCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEEH
DIDLLVRALSMGKLHHAMMGTAAVAIGAAAAVPGTLVHQAAGGGPRNAVPGSVRFGHPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEECCCCC
TLRVGAEAALVNGQWRVTKALMSRSARTLMEGWVRVPPTP
CEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCEECCCCC
>Mature Secondary Structure 
APQIRIPATYLRGGTSKGVFFRLQDLPAAAQQPGAARDALLLRVIGSPDPYGKQIDGMG
CCCCCCCHHHHCCCCCCCEEEEEECCCCHHCCCCCCCCEEEEEEECCCCCCCCCCCCCC
GATSSTSKAVIVSPSTRPGHDVDYLFGQVAIDSPFVDWSGNCGNLSAAVGPFAIASGLVA
CCCCCCCCEEEECCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PGRIPHDGVCTVRIWQANIGKTIVAHVPVAGGQVQETGDFALDGVAFPAAEVLLEFLDPA
CCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCEECCCCEEECCCHHHHHHHHHHHHCCC
DGGEGGEGGGSALFPTGQLVDTLDVPGVGRLQATLINAGIPTIFLNARDVGCTGTELQDA
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHEEEEEECCCCEEEEECCCCCCCCHHHHHH
INGDAAALARFEAIRAWGAVKMGLIHDVAEAATRQHTPKLAFVAPPADYVASSGTAVRAA
HCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEEH
DIDLLVRALSMGKLHHAMMGTAAVAIGAAAAVPGTLVHQAAGGGPRNAVPGSVRFGHPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEECCCCC
TLRVGAEAALVNGQWRVTKALMSRSARTLMEGWVRVPPTP
CEEECCEEEEECCCHHHHHHHHHHHHHHHHHCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA