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The map label for this gene is phnE [H]

Identifier: 222111206

GI number: 222111206

Start: 2152060

End: 2153631

Strand: Direct

Name: phnE [H]

Synonym: Dtpsy_2014

Alternate gene names: 222111206

Gene position: 2152060-2153631 (Clockwise)

Preceding gene: 222111205

Following gene: 222111207

Centisome position: 56.68

GC content: 73.73

Gene sequence:

>1572_bases
ATGCCGTTGACGCCCCCTGCACCGCACCTGCGCGACCCGGCCGCACGCGGGCGGCTGGGCGGCGCGCTGCTGGCCGTGGT
GCTGCTGTGGCCGCTGTTGCAGGCGTCGGGCTTTGACCTGGCCGCGCTGCTGGCGCCCGAGAACCTGCGCGTGCTGGGCG
GGTTCCTGCGCCAGTTTCTGCCGCCTGAAACGGGGGCCGACTTCCTGGCCCTGCTGGCGCGCGCCACCCTGGAAACGCTG
GCGATTGCCACTGCGGGGCTGGCATTGGCCTTCGTGCTGGCGGTGCCGTTGGCCTACTGGGCCAGCAACGCTGCACGCGA
ACGCCCCCGGCTGAACCCGGCAGCGCGCGGCGTGCTCACGGTGCTGCGCGGCATCCCCGAGCTGGTGTGGGCGCTGGTGT
TCGTGCGCGTGTTCGGCCTGGGGCCGACGGCCGGCGTGCTGGCGCTGGGGCTGGCCTACGGCGGCATGCTGGGCAAGGTG
TATGCCGAGATCCTCGAATCCGTGCCCGCCGCACCCGCCCAGGCGCTGGCGCGCAGTGGGGCGCGCCGGCCCATCGCCCT
GCTCTACGGCCTGCTGCCGCAGGCGGCGCGCGAACTCACCTCCTACACCGTGTACCGCTGGGAATGCGCGGTGCGCGCCT
CGGTGGTGATGGGCTTCGTGGGCGCGGGCGGGCTGGGCCAGCTCATGGACCAGGCCATGAAGATGCTCAACGGCGGCGAG
GCGTGCACCATCCTTGTGACCTTCATGCTGCTGGTGCTGGCGGCCGATGCGCTGTCGGGCGGGCTGCGCCGCGCGCTGGA
TGCGCCCCCCGCGGCACGCGCCGCGCCGCTGGGCTGGCGCAGCGGCGCGTTGACCGCGACGGTGCTGGCCGCCCTGGCCG
CCAGCCTGTGGCTGCTGGACATGGACTTGCCCGCGCTCTTCACCGCCGAGGCGGCGCGCAGCATGGCGGAGTTTGCCGGC
GGCTTCTTCCCGCCCGAGCTGGGCAGCACCTGGCTGCGGCAGGTGGCGCGCGGCGTGTGGGAGACGCTGGCCATCTCCAT
CGTGGGCACGCTGCTGGCCGCCCTGCTGGCGCTGCTGCTGGCCCTGCCGCGCCGGCGCGCGGGCTGGAACCTGCTGCTGA
ACCTACTGCGCTCGGTGCCCGAACTGGTATGGGCCACGCTCACCGCCCTGGCCGTGGGCCTGGGGCCGTTTGCCGGCGCA
CTGGCCCTGGCCCTGCACACCACCGGCGTGCTGGGCCGGCTGTATGCCGAGGCGCTGCAGAACACCCCAGCCACGGCCGC
GCGTGCGCTGCGCCAGGCCGGCAGCGGCGGCGCCATGGCGTTCCTGTACGGCACGCTGCCCGGGGCGGCGCCGCAACTGG
TGGCGTACACGCTGTACCGCTGGGAGATGAACATCCGCATGGCCGCCATCCTCGGCTTCGTGGGCGCGGGCGGCCTGGGG
CAGTTGCTTTACGTGGAGCTGTCGCTGTTCCACCATGCGCAGGCGGGCACGGTGATCCTGGCGATGCTGGTGCTATCGGT
TGCGGTGGACTGGGCCAGCGCGGCGCTGCGCCGCTGGATGGACAGGGCCTAG

Upstream 100 bases:

>100_bases
CGCCCGATCTGCTGCAGGCGCTGTACGCCCCGCCCGGCGACGGCGCGCTCCCGCCGGATGAAACGCCCACGCCGCCCCCG
ACCACCAGCAGCTACGCCTC

Downstream 100 bases:

>100_bases
TACCGCAACCGGGGAGCGGAGGAACAAAAGGCGATCGATGGCTTCGCCCCAAGGGTCAGGCGCGAACCGCAGCAACGACC
GTGGCCATTGCGAGGATTGG

Product: binding-protein-dependent transport systems inner membrane component

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPLTPPAPHLRDPAARGRLGGALLAVVLLWPLLQASGFDLAALLAPENLRVLGGFLRQFLPPETGADFLALLARATLETL
AIATAGLALAFVLAVPLAYWASNAARERPRLNPAARGVLTVLRGIPELVWALVFVRVFGLGPTAGVLALGLAYGGMLGKV
YAEILESVPAAPAQALARSGARRPIALLYGLLPQAARELTSYTVYRWECAVRASVVMGFVGAGGLGQLMDQAMKMLNGGE
ACTILVTFMLLVLAADALSGGLRRALDAPPAARAAPLGWRSGALTATVLAALAASLWLLDMDLPALFTAEAARSMAEFAG
GFFPPELGSTWLRQVARGVWETLAISIVGTLLAALLALLLALPRRRAGWNLLLNLLRSVPELVWATLTALAVGLGPFAGA
LALALHTTGVLGRLYAEALQNTPATAARALRQAGSGGAMAFLYGTLPGAAPQLVAYTLYRWEMNIRMAAILGFVGAGGLG
QLLYVELSLFHHAQAGTVILAMLVLSVAVDWASAALRRWMDRA

Sequences:

>Translated_523_residues
MPLTPPAPHLRDPAARGRLGGALLAVVLLWPLLQASGFDLAALLAPENLRVLGGFLRQFLPPETGADFLALLARATLETL
AIATAGLALAFVLAVPLAYWASNAARERPRLNPAARGVLTVLRGIPELVWALVFVRVFGLGPTAGVLALGLAYGGMLGKV
YAEILESVPAAPAQALARSGARRPIALLYGLLPQAARELTSYTVYRWECAVRASVVMGFVGAGGLGQLMDQAMKMLNGGE
ACTILVTFMLLVLAADALSGGLRRALDAPPAARAAPLGWRSGALTATVLAALAASLWLLDMDLPALFTAEAARSMAEFAG
GFFPPELGSTWLRQVARGVWETLAISIVGTLLAALLALLLALPRRRAGWNLLLNLLRSVPELVWATLTALAVGLGPFAGA
LALALHTTGVLGRLYAEALQNTPATAARALRQAGSGGAMAFLYGTLPGAAPQLVAYTLYRWEMNIRMAAILGFVGAGGLG
QLLYVELSLFHHAQAGTVILAMLVLSVAVDWASAALRRWMDRA
>Mature_522_residues
PLTPPAPHLRDPAARGRLGGALLAVVLLWPLLQASGFDLAALLAPENLRVLGGFLRQFLPPETGADFLALLARATLETLA
IATAGLALAFVLAVPLAYWASNAARERPRLNPAARGVLTVLRGIPELVWALVFVRVFGLGPTAGVLALGLAYGGMLGKVY
AEILESVPAAPAQALARSGARRPIALLYGLLPQAARELTSYTVYRWECAVRASVVMGFVGAGGLGQLMDQAMKMLNGGEA
CTILVTFMLLVLAADALSGGLRRALDAPPAARAAPLGWRSGALTATVLAALAASLWLLDMDLPALFTAEAARSMAEFAGG
FFPPELGSTWLRQVARGVWETLAISIVGTLLAALLALLLALPRRRAGWNLLLNLLRSVPELVWATLTALAVGLGPFAGAL
ALALHTTGVLGRLYAEALQNTPATAARALRQAGSGGAMAFLYGTLPGAAPQLVAYTLYRWEMNIRMAAILGFVGAGGLGQ
LLYVELSLFHHAQAGTVILAMLVLSVAVDWASAALRRWMDRA

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 54771; Mature: 54639

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTPPAPHLRDPAARGRLGGALLAVVLLWPLLQASGFDLAALLAPENLRVLGGFLRQFL
CCCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCHHHHHHHHHHHHC
PPETGADFLALLARATLETLAIATAGLALAFVLAVPLAYWASNAARERPRLNPAARGVLT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLRGIPELVWALVFVRVFGLGPTAGVLALGLAYGGMLGKVYAEILESVPAAPAQALARSG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ARRPIALLYGLLPQAARELTSYTVYRWECAVRASVVMGFVGAGGLGQLMDQAMKMLNGGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
ACTILVTFMLLVLAADALSGGLRRALDAPPAARAAPLGWRSGALTATVLAALAASLWLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MDLPALFTAEAARSMAEFAGGFFPPELGSTWLRQVARGVWETLAISIVGTLLAALLALLL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPRRRAGWNLLLNLLRSVPELVWATLTALAVGLGPFAGALALALHTTGVLGRLYAEALQ
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NTPATAARALRQAGSGGAMAFLYGTLPGAAPQLVAYTLYRWEMNIRMAAILGFVGAGGLG
CCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLLYVELSLFHHAQAGTVILAMLVLSVAVDWASAALRRWMDRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLTPPAPHLRDPAARGRLGGALLAVVLLWPLLQASGFDLAALLAPENLRVLGGFLRQFL
CCCCCCCCCCCCHHHCHHHHHHHHHHHHHHHHHCCCCCEEEHCCCCHHHHHHHHHHHHC
PPETGADFLALLARATLETLAIATAGLALAFVLAVPLAYWASNAARERPRLNPAARGVLT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLRGIPELVWALVFVRVFGLGPTAGVLALGLAYGGMLGKVYAEILESVPAAPAQALARSG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
ARRPIALLYGLLPQAARELTSYTVYRWECAVRASVVMGFVGAGGLGQLMDQAMKMLNGGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
ACTILVTFMLLVLAADALSGGLRRALDAPPAARAAPLGWRSGALTATVLAALAASLWLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
MDLPALFTAEAARSMAEFAGGFFPPELGSTWLRQVARGVWETLAISIVGTLLAALLALLL
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPRRRAGWNLLLNLLRSVPELVWATLTALAVGLGPFAGALALALHTTGVLGRLYAEALQ
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NTPATAARALRQAGSGGAMAFLYGTLPGAAPQLVAYTLYRWEMNIRMAAILGFVGAGGLG
CCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
QLLYVELSLFHHAQAGTVILAMLVLSVAVDWASAALRRWMDRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA