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The map label for this gene is def2 [H]

Identifier: 222110774

GI number: 222110774

Start: 1665506

End: 1666045

Strand: Direct

Name: def2 [H]

Synonym: Dtpsy_1579

Alternate gene names: 222110774

Gene position: 1665506-1666045 (Clockwise)

Preceding gene: 222110771

Following gene: 222110777

Centisome position: 43.87

GC content: 65.93

Gene sequence:

>540_bases
ATGACCATCCGCACCATCCTGAAGATGGGCGACCCGCGACTGTTGCGGGTGGCCCAACCCGTTACCGCATTCGATACGCC
CGCGCTGCACCAACTGGTGAACGACATGCGCGAGACGATGCAGGCCGTGAACGGCGCGGGCCTGGCAGCACCGCAGATCG
GGGAGGATTGGCAGGTGGTGATCTTCGGCTCCGGCGAGCGCAACCCGCGCTATCCCGACCGCCCCATCGTGCCGCCCACG
GTGCTCATCAATCCCGTGATCACGCCGCTGGGCGCCGACGAGGAGGACGACTGGGAGGGCTGCCTGTCCGTGCCGGGCCT
GCGCGGCAAGGTGCCGCGCTTTGCGCGCATCCACTACCGCGGCGTCGATCTGCTGGGCGCGCCCATCGATCGTGTGGCGG
AAGGTTTCCACGCGCGGGTAGTGCAGCACGAGTGCGACCACCTGATCGGCAAGCTCTACCCAATGCGCGTGCGCGATTTC
ACGCAGTTCGGTTACACGGAGGTGTTGTTTCCGGAGATTGGTGCGGTAGAAGACGATTGA

Upstream 100 bases:

>100_bases
CCATTGTGCAGGTGTTGCCGGGCGTTATGCTCCAAGGCAGGGACGCCGCCATGCCTTTCGCCATGCCCTGCGCTTGGCGG
CTGCAGCCCGCAGACCCACC

Downstream 100 bases:

>100_bases
GCGCTCAATCAGGCTGTAGCGCCCTTGTGGCAAGCGCAAGCAGCTATCGAAATCATAGTCATTGATCGGCCAGCGCCTGC
AGTAGCTCGGTCTCGATCTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MTIRTILKMGDPRLLRVAQPVTAFDTPALHQLVNDMRETMQAVNGAGLAAPQIGEDWQVVIFGSGERNPRYPDRPIVPPT
VLINPVITPLGADEEDDWEGCLSVPGLRGKVPRFARIHYRGVDLLGAPIDRVAEGFHARVVQHECDHLIGKLYPMRVRDF
TQFGYTEVLFPEIGAVEDD

Sequences:

>Translated_179_residues
MTIRTILKMGDPRLLRVAQPVTAFDTPALHQLVNDMRETMQAVNGAGLAAPQIGEDWQVVIFGSGERNPRYPDRPIVPPT
VLINPVITPLGADEEDDWEGCLSVPGLRGKVPRFARIHYRGVDLLGAPIDRVAEGFHARVVQHECDHLIGKLYPMRVRDF
TQFGYTEVLFPEIGAVEDD
>Mature_178_residues
TIRTILKMGDPRLLRVAQPVTAFDTPALHQLVNDMRETMQAVNGAGLAAPQIGEDWQVVIFGSGERNPRYPDRPIVPPTV
LINPVITPLGADEEDDWEGCLSVPGLRGKVPRFARIHYRGVDLLGAPIDRVAEGFHARVVQHECDHLIGKLYPMRVRDFT
QFGYTEVLFPEIGAVEDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=33.7142857142857, Blast_Score=73, Evalue=1e-13,
Organism=Escherichia coli, GI1789682, Length=154, Percent_Identity=37.012987012987, Blast_Score=92, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24645728, Length=184, Percent_Identity=30.9782608695652, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19935; Mature: 19804

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRTILKMGDPRLLRVAQPVTAFDTPALHQLVNDMRETMQAVNGAGLAAPQIGEDWQVV
CCHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IFGSGERNPRYPDRPIVPPTVLINPVITPLGADEEDDWEGCLSVPGLRGKVPRFARIHYR
EEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEC
GVDLLGAPIDRVAEGFHARVVQHECDHLIGKLYPMRVRDFTQFGYTEVLFPEIGAVEDD
CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TIRTILKMGDPRLLRVAQPVTAFDTPALHQLVNDMRETMQAVNGAGLAAPQIGEDWQVV
CHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IFGSGERNPRYPDRPIVPPTVLINPVITPLGADEEDDWEGCLSVPGLRGKVPRFARIHYR
EEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEEEEEC
GVDLLGAPIDRVAEGFHARVVQHECDHLIGKLYPMRVRDFTQFGYTEVLFPEIGAVEDD
CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]