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The map label for this gene is rlmE [H]

Identifier: 222110672

GI number: 222110672

Start: 1545244

End: 1545906

Strand: Direct

Name: rlmE [H]

Synonym: Dtpsy_1474

Alternate gene names: 222110672

Gene position: 1545244-1545906 (Clockwise)

Preceding gene: 222110669

Following gene: 222110673

Centisome position: 40.7

GC content: 61.24

Gene sequence:

>663_bases
ATGAAAGCCCAGACCAAAAGCAAGAAGGTGAACAAGGCATGGCTTCACGACCATGTCAACGACACCTACGTCAAGCTCGC
CCAGAAGGAGGGCTACCGCGCACGCGCCGCCTACAAACTCAAGGAGATCGACGAACAGCTCGGGCTGATCAAGCCCGGCC
ATGTGGTGGTGGATCTCGGGTCGTCTCCGGGGGCCTGGAGCCAGTATGTGCGCCGGCGGCTGTCGCCGGACGGGGCTGCA
GTGGGGCAACTCAACGGCGTCATCATCGCGCTGGACATTCTGCCGATGGAACCCATTGAAGGCGTCACCTTTTTACAGGG
GGATTTTCGGGAGGAAGAGGTGCTGGCACGCTTGCAGGAGGCCGTGCAAGGGCGGCCCGTGGATGTGGTGGTGTCGGACA
TGGCTCCCAATCTGTCGGGCGTGGAGTCGGTGGATGCCGTGCGTATCGCGCACCTGATCGAGCTGGCGGTGGACTTTGCC
GTGCACCATCTGAAGCCTGAGGGGGCACTGGTTGTGAAGCTGTTCCACGGCAGCGGTTACTCCCAGCTGGTGCAACTCTT
CAAGGACACCTTTCGCGTGGTCAAGCCGATGAAGCCCAAGGCCTCGCGCGACAAATCATCGGAGACCTTTCTGGTGGGCA
TGGGGCTCAAGCGTCAAGGTTGA

Upstream 100 bases:

>100_bases
GGAGTCAGTTGAATTTGAGGCATACCCGTATTATCTCAACGCTCCGGCAATGGCCGGCATACTGCTGTCCCCCGATCCGG
GGCGGCGCACCGATCTATCC

Downstream 100 bases:

>100_bases
CCGCAGCGCCTTGACCATGGCCTGTGCCGGGGTTAATGGGCGGTGCTGGCGGGTTGAAACGCCTAAAATGAACCCTAATT
GCGTTAGGACGTTTCCATGA

Product: ribosomal RNA methyltransferase rrmj/ftsj

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MKAQTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDEQLGLIKPGHVVVDLGSSPGAWSQYVRRRLSPDGAA
VGQLNGVIIALDILPMEPIEGVTFLQGDFREEEVLARLQEAVQGRPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFA
VHHLKPEGALVVKLFHGSGYSQLVQLFKDTFRVVKPMKPKASRDKSSETFLVGMGLKRQG

Sequences:

>Translated_220_residues
MKAQTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDEQLGLIKPGHVVVDLGSSPGAWSQYVRRRLSPDGAA
VGQLNGVIIALDILPMEPIEGVTFLQGDFREEEVLARLQEAVQGRPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFA
VHHLKPEGALVVKLFHGSGYSQLVQLFKDTFRVVKPMKPKASRDKSSETFLVGMGLKRQG
>Mature_220_residues
MKAQTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDEQLGLIKPGHVVVDLGSSPGAWSQYVRRRLSPDGAA
VGQLNGVIIALDILPMEPIEGVTFLQGDFREEEVLARLQEAVQGRPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFA
VHHLKPEGALVVKLFHGSGYSQLVQLFKDTFRVVKPMKPKASRDKSSETFLVGMGLKRQG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=201, Percent_Identity=35.3233830845771, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI29029591, Length=195, Percent_Identity=35.3846153846154, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI29029589, Length=195, Percent_Identity=35.3846153846154, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI7110661, Length=195, Percent_Identity=35.3846153846154, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI194097365, Length=196, Percent_Identity=31.6326530612245, Blast_Score=117, Evalue=6e-27,
Organism=Escherichia coli, GI1789569, Length=204, Percent_Identity=47.0588235294118, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17554650, Length=194, Percent_Identity=34.5360824742268, Blast_Score=123, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17553474, Length=221, Percent_Identity=33.4841628959276, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71987561, Length=193, Percent_Identity=34.1968911917098, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17553860, Length=193, Percent_Identity=34.1968911917098, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71987550, Length=192, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319535, Length=201, Percent_Identity=35.3233830845771, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319796, Length=198, Percent_Identity=35.3535353535354, Blast_Score=118, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321302, Length=98, Percent_Identity=39.7959183673469, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=35.3211009174312, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI18859957, Length=195, Percent_Identity=30.7692307692308, Blast_Score=110, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=34.1968911917098, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=34.3434343434343, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24245; Mature: 24245

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAQTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDEQLGLIKPGHVVVDLG
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECC
SSPGAWSQYVRRRLSPDGAAVGQLNGVIIALDILPMEPIEGVTFLQGDFREEEVLARLQE
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHH
AVQGRPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFAVHHLKPEGALVVKLFHGSGY
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQLVQLFKDTFRVVKPMKPKASRDKSSETFLVGMGLKRQG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKAQTKSKKVNKAWLHDHVNDTYVKLAQKEGYRARAAYKLKEIDEQLGLIKPGHVVVDLG
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECC
SSPGAWSQYVRRRLSPDGAAVGQLNGVIIALDILPMEPIEGVTFLQGDFREEEVLARLQE
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHH
AVQGRPVDVVVSDMAPNLSGVESVDAVRIAHLIELAVDFAVHHLKPEGALVVKLFHGSGY
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQLVQLFKDTFRVVKPMKPKASRDKSSETFLVGMGLKRQG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA