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The map label for this gene is ynfL [H]

Identifier: 222110515

GI number: 222110515

Start: 1374218

End: 1375144

Strand: Direct

Name: ynfL [H]

Synonym: Dtpsy_1314

Alternate gene names: 222110515

Gene position: 1374218-1375144 (Clockwise)

Preceding gene: 222110514

Following gene: 222110516

Centisome position: 36.2

GC content: 59.01

Gene sequence:

>927_bases
GTGGAGTTACGCCACCTTCGATGTTTTACCGCGGTCGCGGAAGAGCTTCATTTTGCTCGGGCTGCTGAACGGTTACACAT
CGACCAATCGCCGCTATCTCGCACCATCAAGGAGCTGGAGGAAGACCTTGGTGCGCGCCTGTTCGTTCGTACTACCCGCA
GCACGCAATTGACCCGTGCGGGTCGATTGCTGTTGGAGCACGTACCGCGCATCTTCGCTGCGCTGGAGCAAGCACGCGAT
AGCGTCAGGTCTGCCTCCAATGGCTATCACGGGCAATTGCGCATTGCCTTGTCCGATGGCATCACGCCATCACGTTTACC
GGCGCTACTTGCGCAGTGCCGCGAAGAAGACCCCGAAGTCGAGGTTCGGTTGTTCGAGGTTTCGATGGCCCAGCAAATCA
AGGGCCTGCACGATGAGCTATACGACGCAGGATTTTCGATGGCCGACTACGTGGGCGATGGCATCGTGGTCACATCTGCC
TGGGAGGACGAATTGATGGTGGCAGTACCTGCCCGCCATCCCGTGCTTGCCTTCAAGCAGGTTCCGCTGGAGGAAGTGCT
GCACTATCCGCTGGTGTTGGGCGATCCGGCGATATGCGAAGGCCATGCGCGCCAGGTCGATCGCATCCTGCGCAGGCACG
AGCAGGAGCCGCTGATTGTTCAGCATGTTGCCACCTTTGACCTCATGATGACCTTGGTTTCCGCCGGATTGGCATTGGGC
CTGGCTGGTGCGGCGCACATCGGCTCCAGCCGCGAGCTCGGTGTCGTGGCTCGGCCTTTGGCGGGTAAACCACCGATGCT
GACGACCTATCTGCTGCGTCGTGATGCGGAGGTTCCAGAAATGTTGGCACGGTTCATCGAACGAGTGGCCTTCATTGACT
CGGAAAATTATCTGAGCACCGCCAAAAATTCCCGACCCACCCGATGA

Upstream 100 bases:

>100_bases
TCGGTTTGGAAGGACAGCGTTCTTGGTGTTCTCACATGCGTGGAGCATCCGGCTGCATCCCACGGGACGTGGACGATCTG
ACACGCTAGGAAGGGCAGAA

Downstream 100 bases:

>100_bases
AAGGACTGAAACCATGAGCAAGGTGATGCTGCTGATGCTGGCCGCCACACTGACTGCATGTGGCCCTTCTCAACCTTCGG
AAACGGTGGATGCACTCGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MELRHLRCFTAVAEELHFARAAERLHIDQSPLSRTIKELEEDLGARLFVRTTRSTQLTRAGRLLLEHVPRIFAALEQARD
SVRSASNGYHGQLRIALSDGITPSRLPALLAQCREEDPEVEVRLFEVSMAQQIKGLHDELYDAGFSMADYVGDGIVVTSA
WEDELMVAVPARHPVLAFKQVPLEEVLHYPLVLGDPAICEGHARQVDRILRRHEQEPLIVQHVATFDLMMTLVSAGLALG
LAGAAHIGSSRELGVVARPLAGKPPMLTTYLLRRDAEVPEMLARFIERVAFIDSENYLSTAKNSRPTR

Sequences:

>Translated_308_residues
MELRHLRCFTAVAEELHFARAAERLHIDQSPLSRTIKELEEDLGARLFVRTTRSTQLTRAGRLLLEHVPRIFAALEQARD
SVRSASNGYHGQLRIALSDGITPSRLPALLAQCREEDPEVEVRLFEVSMAQQIKGLHDELYDAGFSMADYVGDGIVVTSA
WEDELMVAVPARHPVLAFKQVPLEEVLHYPLVLGDPAICEGHARQVDRILRRHEQEPLIVQHVATFDLMMTLVSAGLALG
LAGAAHIGSSRELGVVARPLAGKPPMLTTYLLRRDAEVPEMLARFIERVAFIDSENYLSTAKNSRPTR
>Mature_308_residues
MELRHLRCFTAVAEELHFARAAERLHIDQSPLSRTIKELEEDLGARLFVRTTRSTQLTRAGRLLLEHVPRIFAALEQARD
SVRSASNGYHGQLRIALSDGITPSRLPALLAQCREEDPEVEVRLFEVSMAQQIKGLHDELYDAGFSMADYVGDGIVVTSA
WEDELMVAVPARHPVLAFKQVPLEEVLHYPLVLGDPAICEGHARQVDRILRRHEQEPLIVQHVATFDLMMTLVSAGLALG
LAGAAHIGSSRELGVVARPLAGKPPMLTTYLLRRDAEVPEMLARFIERVAFIDSENYLSTAKNSRPTR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=246, Percent_Identity=34.5528455284553, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=31.3207547169811, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=30, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1790208, Length=249, Percent_Identity=30.1204819277108, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787601, Length=292, Percent_Identity=24.6575342465753, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI145693105, Length=192, Percent_Identity=28.6458333333333, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI157672245, Length=275, Percent_Identity=29.8181818181818, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786713, Length=144, Percent_Identity=37.5, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790399, Length=199, Percent_Identity=25.1256281407035, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787806, Length=299, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=29.3388429752066, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788297, Length=149, Percent_Identity=32.8859060402685, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787949, Length=249, Percent_Identity=25.7028112449799, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787589, Length=147, Percent_Identity=31.9727891156463, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=27.4590163934426, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=31.1475409836066, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082132, Length=124, Percent_Identity=29.8387096774194, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994962, Length=145, Percent_Identity=31.0344827586207, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788481, Length=232, Percent_Identity=28.448275862069, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787530, Length=251, Percent_Identity=25.8964143426295, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34348; Mature: 34348

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRCFTAVAEELHFARAAERLHIDQSPLSRTIKELEEDLGARLFVRTTRSTQLTRA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
GRLLLEHVPRIFAALEQARDSVRSASNGYHGQLRIALSDGITPSRLPALLAQCREEDPEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCE
EVRLFEVSMAQQIKGLHDELYDAGFSMADYVGDGIVVTSAWEDELMVAVPARHPVLAFKQ
EEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCEEEEECCCCCHHHHHC
VPLEEVLHYPLVLGDPAICEGHARQVDRILRRHEQEPLIVQHVATFDLMMTLVSAGLALG
CCHHHHHHCCEEECCCHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAGAAHIGSSRELGVVARPLAGKPPMLTTYLLRRDAEVPEMLARFIERVAFIDSENYLST
HHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
AKNSRPTR
HCCCCCCC
>Mature Secondary Structure
MELRHLRCFTAVAEELHFARAAERLHIDQSPLSRTIKELEEDLGARLFVRTTRSTQLTRA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHH
GRLLLEHVPRIFAALEQARDSVRSASNGYHGQLRIALSDGITPSRLPALLAQCREEDPEV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCE
EVRLFEVSMAQQIKGLHDELYDAGFSMADYVGDGIVVTSAWEDELMVAVPARHPVLAFKQ
EEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCEEEEECCCCCHHHHHC
VPLEEVLHYPLVLGDPAICEGHARQVDRILRRHEQEPLIVQHVATFDLMMTLVSAGLALG
CCHHHHHHCCEEECCCHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LAGAAHIGSSRELGVVARPLAGKPPMLTTYLLRRDAEVPEMLARFIERVAFIDSENYLST
HHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHH
AKNSRPTR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]