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The map label for this gene is nusA [H]

Identifier: 222110449

GI number: 222110449

Start: 1308135

End: 1309619

Strand: Direct

Name: nusA [H]

Synonym: Dtpsy_1246

Alternate gene names: 222110449

Gene position: 1308135-1309619 (Clockwise)

Preceding gene: 222110448

Following gene: 222110450

Centisome position: 34.46

GC content: 61.75

Gene sequence:

>1485_bases
ATGAATCGCGAGTTGTTGATGTTGGTAGAAGCCATTTCGCGCGAAAAGAACGTGGAGCGCGATGTGGTCCTGGGCGCTGT
GGAGTCTGCTCTGGCACAGGCCACGAAGAAGCTCTACCAGGGTGAAGTGGACATCCGCGTGTCGATTGATCGCGACAGCG
GCGAATACGAGACTTTCCGTCGTTGGCTGGTGGTGCCCGACGATGCCGGATTGCAGAACCCCGACGCCGAAGAGCTGCTG
ATGGATGCCCGCGAACGGGTGGCCGATATCGAGGTGGGGGAGTACATCGAGGAAGCCGTCGAGTCGGTACCGATCGGCCG
CATCGGGGCCATGGCGGCCAAGCAGGTGATCCTGCAAAAGATCCGCGATGCCGAACGCGAGATGCTGCTCAACGATTTCA
TGTCACGCGGCGAGAAAATTTTCACCGGCACGGTGAAACGCATGGACAAGGGCGACATCATCGTCGAGAGCGGCCGGGTG
GAAGGGCGCCTGCGTCGCGGCGAGATGATCCCGAAGGAAAACCTGCGCAATGGGGACCGTGTGCGCGCCATGATCATGGA
GGTGGACCTCACGCTGCGCGGAGCGCCCATCATCCTCTCGCGCTCCGCGCCGGAGTTCATGATCGAGTTGTTCCGCAACG
AGGTGCCCGAGATCGAACAGGGTCTGCTGGAGATCAAAAGCTGTGCCCGTGACCCCGGCAGCCGCGCGAAGATCGCTGTG
CTCAGCCATGACAAGCGCGTGGACCCCATCGGCACCTGCGTGGGCGTGCGCGGAACGCGTGTGAACGCCGTGACCAACGA
ACTGGCAGGAGAGCGTGTGGACATCGTGCTGTGGTCCGACGACCCTGCGCAGTTCGTGATCGGCGCGCTTGCTCCGGCCA
ACGTGACTTCCATCGTGGTCGACGAAGAGAAGCACGCCATGGACGTCGTGGTGGACGAGGAAAACCTCGCCATCGCCATC
GGGCGCGGCGGCCAGAACGTGCGCCTGGCGTCCGACCTCACGGGCTGGAAGATCAACATCATGGATGCGGCCGAATCCGC
GCAGAAGCAGGCCAATGAAACCGATGCCGCGCGTCGCCTGTTCATGGAGAAGCTGGATGTGGATGAGGAGATCGCCAACA
TCCTGATCGAGGAGGGCTTTGCCAGCCTCGAGGAAGTGGCCTATGTGCCGCTGCAGGAAATGCTGGAGATCGAGAGCTTC
GACGAGGACACGGTGAACGAACTGCGCGCACGCGCCAAGGATGCGCTGCTCACCATGGAAATCGTTCGTGAAGAAAGCGT
GGAAAGCGTATCGCAAGACCTGCGTGACCTCGAAGGTCTGACGCCCGAACTGATCGCCAAGTTGGCGGAAGGCGGCGTGC
ATACGCGTGACGACCTGGCCGATTTGGCCATTGACGAACTGACCGAGCTGACCGGCCAGTCTGCCGAAGATGCGACCGCC
TTGATCATGAAGGCGCGCGAGCACTGGTTCACGGGGCAAGAGTAA

Upstream 100 bases:

>100_bases
ACGAGGGACCAAGCCCGGAGAGCCGGGCTGAAGGTTGCATAACCGGACTGTCCTCTGAACGGGCAGTTCGTCAGGAATTG
AGAAACAGGAGAGCCGTCGC

Downstream 100 bases:

>100_bases
GGGTCAGGGAGGTTCGAACACAATATGTCGAGTAACACTGTTGCTGAGTTCGCTGCAGAGCTCAAGAAATCGCCTGAAAC
CTTGCTGGACCAGCTGAAGT

Product: nusa antitermination factor

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNRELLMLVEAISREKNVERDVVLGAVESALAQATKKLYQGEVDIRVSIDRDSGEYETFRRWLVVPDDAGLQNPDAEELL
MDARERVADIEVGEYIEEAVESVPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRV
EGRLRRGEMIPKENLRNGDRVRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSDDPAQFVIGALAPANVTSIVVDEEKHAMDVVVDEENLAIAI
GRGGQNVRLASDLTGWKINIMDAAESAQKQANETDAARRLFMEKLDVDEEIANILIEEGFASLEEVAYVPLQEMLEIESF
DEDTVNELRARAKDALLTMEIVREESVESVSQDLRDLEGLTPELIAKLAEGGVHTRDDLADLAIDELTELTGQSAEDATA
LIMKAREHWFTGQE

Sequences:

>Translated_494_residues
MNRELLMLVEAISREKNVERDVVLGAVESALAQATKKLYQGEVDIRVSIDRDSGEYETFRRWLVVPDDAGLQNPDAEELL
MDARERVADIEVGEYIEEAVESVPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRV
EGRLRRGEMIPKENLRNGDRVRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSDDPAQFVIGALAPANVTSIVVDEEKHAMDVVVDEENLAIAI
GRGGQNVRLASDLTGWKINIMDAAESAQKQANETDAARRLFMEKLDVDEEIANILIEEGFASLEEVAYVPLQEMLEIESF
DEDTVNELRARAKDALLTMEIVREESVESVSQDLRDLEGLTPELIAKLAEGGVHTRDDLADLAIDELTELTGQSAEDATA
LIMKAREHWFTGQE
>Mature_494_residues
MNRELLMLVEAISREKNVERDVVLGAVESALAQATKKLYQGEVDIRVSIDRDSGEYETFRRWLVVPDDAGLQNPDAEELL
MDARERVADIEVGEYIEEAVESVPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRV
EGRLRRGEMIPKENLRNGDRVRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSDDPAQFVIGALAPANVTSIVVDEEKHAMDVVVDEENLAIAI
GRGGQNVRLASDLTGWKINIMDAAESAQKQANETDAARRLFMEKLDVDEEIANILIEEGFASLEEVAYVPLQEMLEIESF
DEDTVNELRARAKDALLTMEIVREESVESVSQDLRDLEGLTPELIAKLAEGGVHTRDDLADLAIDELTELTGQSAEDATA
LIMKAREHWFTGQE

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=494, Percent_Identity=51.8218623481781, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54902; Mature: 54902

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRELLMLVEAISREKNVERDVVLGAVESALAQATKKLYQGEVDIRVSIDRDSGEYETFR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHH
RWLVVPDDAGLQNPDAEELLMDARERVADIEVGEYIEEAVESVPIGRIGAMAAKQVILQK
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRVEGRLRRGEMIPKENLRNGDR
HHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEECCCCCCHHHCCCCCCHHHCCCCHH
VRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
HEEEHEEECEEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEE
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSDDPAQFVIGALAPANVTSIVV
EECCCCCCHHHHEECCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEE
DEEKHAMDVVVDEENLAIAIGRGGQNVRLASDLTGWKINIMDAAESAQKQANETDAARRL
ECCCCCEEEEEECCCEEEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHCCHHHHHHHH
FMEKLDVDEEIANILIEEGFASLEEVAYVPLQEMLEIESFDEDTVNELRARAKDALLTME
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IVREESVESVSQDLRDLEGLTPELIAKLAEGGVHTRDDLADLAIDELTELTGQSAEDATA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
LIMKAREHWFTGQE
HHHHHHHHCCCCCC
>Mature Secondary Structure
MNRELLMLVEAISREKNVERDVVLGAVESALAQATKKLYQGEVDIRVSIDRDSGEYETFR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHH
RWLVVPDDAGLQNPDAEELLMDARERVADIEVGEYIEEAVESVPIGRIGAMAAKQVILQK
EEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDAEREMLLNDFMSRGEKIFTGTVKRMDKGDIIVESGRVEGRLRRGEMIPKENLRNGDR
HHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCEEEECCCCCCHHHCCCCCCHHHCCCCHH
VRAMIMEVDLTLRGAPIILSRSAPEFMIELFRNEVPEIEQGLLEIKSCARDPGSRAKIAV
HEEEHEEECEEECCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEE
LSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIVLWSDDPAQFVIGALAPANVTSIVV
EECCCCCCHHHHEECCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCCCCEEEEE
DEEKHAMDVVVDEENLAIAIGRGGQNVRLASDLTGWKINIMDAAESAQKQANETDAARRL
ECCCCCEEEEEECCCEEEEEECCCCCEEEECCCCCCEEEEEHHHHHHHHHCCHHHHHHHH
FMEKLDVDEEIANILIEEGFASLEEVAYVPLQEMLEIESFDEDTVNELRARAKDALLTME
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IVREESVESVSQDLRDLEGLTPELIAKLAEGGVHTRDDLADLAIDELTELTGQSAEDATA
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
LIMKAREHWFTGQE
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]