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The map label for this gene is acrA [H]

Identifier: 222110361

GI number: 222110361

Start: 1212422

End: 1213729

Strand: Direct

Name: acrA [H]

Synonym: Dtpsy_1152

Alternate gene names: 222110361

Gene position: 1212422-1213729 (Clockwise)

Preceding gene: 222110360

Following gene: 222110362

Centisome position: 31.93

GC content: 71.48

Gene sequence:

>1308_bases
ATGCGTAAAAAAATCAGAATCATTGTGGTGGCAGGCGTCATCGCAGCCGTCGGGGGGATAGCGATCGCTGCCCGCGACGC
CAAGGACGAGGGCAAGGCCGCGGCGCCCCCGGCGGGCGCGCCGATGGCGCCGCAGGTGCCCGTGGCCGAGGTCATTACCC
GCACGGTCGCGCCATCGGCCGAGTACACCGGCTTCCTGGCCGCGCCCAAGACCGTCGAGCTGCGCTCGCGGGTCGGCGGC
GCCGTCGATGCGGTGAGCGTTCCCGAAGGTAGCCTCGTGCGCAAGGGCCAACTGCTGTTCCAGATCGACCCGAGGCCGTT
CCAGGTTGCGCTCGACACGGCCGTGGCGCAACTGCGTCAGGCCGAGGTGCTGGCCAGCCAGGCCCAGGCCGACTTTGACC
GCGCCGAGCGCCTGGTGGCGACCGGCGCCGTCGCGCGCAAGACCTATGACGATGCCGCCTCCGCACGCAACGCGCGCCAG
GCTCAGGTGCAGGCGGCTAAGGCCGCCGTTGCTGCGGCTCAGCTGGACCTGTCCTACGCCCGCGTCACTGCGCCCATCGC
CGGGCGCGTCGACCGCGTGCTCGTGACCGAGGGCAACTTGGTCAGCGGTGGCGGCGCCGGCGCGGCCACGCTGCTGACCA
CGATCGTATCCATCGACCCGTTGCACGTGTACTTCGACATTGATGAGGCCACCTATCTCAACGTCGTCAGCCGCTCGCGG
CCCGCTGCGGGCGCCGGTAGCAAGGCTTCGCTGCCCGTGCAGGTCGGGCTGACCACGGACAAGGGTTTCCCGCACAGGGG
CGCGCTCGACTTCGTGGGCAACACCATTGACCGAAGCACCGGCACGATCCGCGCGCGCGCCGTTGTGCCCAACCCGGATG
GGCGCATGGCACCCGGCATGTTCGCCCGGGCCAAGCTGTCCACCGGCGCGGCGCGCGAGGCCGTCCTGATCGACGATCAG
GCCGTGGGCACCGACCAAGGGCGCAACTACGTGCTGGTCGTGGGCGAGAACAACCAGGCCCAGTACCGGCCCATCGAACT
GGGGCCGGTGGTGGACGGGCTGCGCGTCATCAACGGCGGCCTGCAGGCCGGTGAGAAGATCATCATCAAGGGCCTCGTGC
GCCCCGGCATGGCGGTCACGCCGCGCATGGTGCCCATGCAGGCGCCCGCGGCACCGGCCGCCGGCGCGACCGGCGCGACC
AAGGCTGCGGCAAACGCTCCGGCTCCCGCTGCGGCGCCTGCCAAGGCCGGTGGCGCGGACCCCGCCAAGGCTGACGGCGC
GGCCGGCTCGGCGGAGGCCCGCAAATGA

Upstream 100 bases:

>100_bases
GGCTTGACCTTGCCGTCGTTGTAACGCCTAGCCTCCACACAAATGTCTGCAGAGCGCTTCGGAAGGGCCGGAGTCCTGCA
TCCAAGAAAGGGTCACTTCC

Downstream 100 bases:

>100_bases
ATTTCCCCCGCTTCTTCATCAACCGGCCGATCTTCGCCATCGTCCTGTCGGTGCTGATGCTGATCGCGGGCGCGATCAGC
TATTTCCAGCTCCCCCTGAG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRKKIRIIVVAGVIAAVGGIAIAARDAKDEGKAAAPPAGAPMAPQVPVAEVITRTVAPSAEYTGFLAAPKTVELRSRVGG
AVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTAVAQLRQAEVLASQAQADFDRAERLVATGAVARKTYDDAASARNARQ
AQVQAAKAAVAAAQLDLSYARVTAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPLHVYFDIDEATYLNVVSRSR
PAAGAGSKASLPVQVGLTTDKGFPHRGALDFVGNTIDRSTGTIRARAVVPNPDGRMAPGMFARAKLSTGAAREAVLIDDQ
AVGTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQAGEKIIIKGLVRPGMAVTPRMVPMQAPAAPAAGATGAT
KAAANAPAPAAAPAKAGGADPAKADGAAGSAEARK

Sequences:

>Translated_435_residues
MRKKIRIIVVAGVIAAVGGIAIAARDAKDEGKAAAPPAGAPMAPQVPVAEVITRTVAPSAEYTGFLAAPKTVELRSRVGG
AVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTAVAQLRQAEVLASQAQADFDRAERLVATGAVARKTYDDAASARNARQ
AQVQAAKAAVAAAQLDLSYARVTAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPLHVYFDIDEATYLNVVSRSR
PAAGAGSKASLPVQVGLTTDKGFPHRGALDFVGNTIDRSTGTIRARAVVPNPDGRMAPGMFARAKLSTGAAREAVLIDDQ
AVGTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQAGEKIIIKGLVRPGMAVTPRMVPMQAPAAPAAGATGAT
KAAANAPAPAAAPAKAGGADPAKADGAAGSAEARK
>Mature_435_residues
MRKKIRIIVVAGVIAAVGGIAIAARDAKDEGKAAAPPAGAPMAPQVPVAEVITRTVAPSAEYTGFLAAPKTVELRSRVGG
AVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTAVAQLRQAEVLASQAQADFDRAERLVATGAVARKTYDDAASARNARQ
AQVQAAKAAVAAAQLDLSYARVTAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPLHVYFDIDEATYLNVVSRSR
PAAGAGSKASLPVQVGLTTDKGFPHRGALDFVGNTIDRSTGTIRARAVVPNPDGRMAPGMFARAKLSTGAAREAVLIDDQ
AVGTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGGLQAGEKIIIKGLVRPGMAVTPRMVPMQAPAAPAAGATGAT
KAAANAPAPAAAPAKAGGADPAKADGAAGSAEARK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=33.7912087912088, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1786668, Length=401, Percent_Identity=33.9152119700748, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1789665, Length=386, Percent_Identity=34.1968911917098, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87082036, Length=357, Percent_Identity=30.2521008403361, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87081796, Length=333, Percent_Identity=28.5285285285285, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44188; Mature: 44188

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKIRIIVVAGVIAAVGGIAIAARDAKDEGKAAAPPAGAPMAPQVPVAEVITRTVAPSA
CCCEEEEEEEEHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
EYTGFLAAPKTVELRSRVGGAVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTAVAQLRQ
CCCEEEECCCHHHHHHHCCCCEEEEECCCCCEEECCCEEEEECCCCEEEHHHHHHHHHHH
AEVLASQAQADFDRAERLVATGAVARKTYDDAASARNARQAQVQAAKAAVAAAQLDLSYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHH
RVTAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPLHVYFDIDEATYLNVVSRSR
HEECCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHCCC
PAAGAGSKASLPVQVGLTTDKGFPHRGALDFVGNTIDRSTGTIRARAVVPNPDGRMAPGM
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCH
FARAKLSTGAAREAVLIDDQAVGTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGG
HEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHCC
LQAGEKIIIKGLVRPGMAVTPRMVPMQAPAAPAAGATGATKAAANAPAPAAAPAKAGGAD
HHCCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
PAKADGAAGSAEARK
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKKIRIIVVAGVIAAVGGIAIAARDAKDEGKAAAPPAGAPMAPQVPVAEVITRTVAPSA
CCCEEEEEEEEHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
EYTGFLAAPKTVELRSRVGGAVDAVSVPEGSLVRKGQLLFQIDPRPFQVALDTAVAQLRQ
CCCEEEECCCHHHHHHHCCCCEEEEECCCCCEEECCCEEEEECCCCEEEHHHHHHHHHHH
AEVLASQAQADFDRAERLVATGAVARKTYDDAASARNARQAQVQAAKAAVAAAQLDLSYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHH
RVTAPIAGRVDRVLVTEGNLVSGGGAGAATLLTTIVSIDPLHVYFDIDEATYLNVVSRSR
HEECCCCCCCEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHCCC
PAAGAGSKASLPVQVGLTTDKGFPHRGALDFVGNTIDRSTGTIRARAVVPNPDGRMAPGM
CCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCH
FARAKLSTGAAREAVLIDDQAVGTDQGRNYVLVVGENNQAQYRPIELGPVVDGLRVINGG
HEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHCC
LQAGEKIIIKGLVRPGMAVTPRMVPMQAPAAPAAGATGATKAAANAPAPAAAPAKAGGAD
HHCCCEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCC
PAKADGAAGSAEARK
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]