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The map label for this gene is rsuA [H]

Identifier: 222110316

GI number: 222110316

Start: 1156578

End: 1157315

Strand: Direct

Name: rsuA [H]

Synonym: Dtpsy_1103

Alternate gene names: 222110316

Gene position: 1156578-1157315 (Clockwise)

Preceding gene: 222110315

Following gene: 222110317

Centisome position: 30.46

GC content: 59.49

Gene sequence:

>738_bases
ATGCAGCTTCAGGACATTCTCTATTCGCAAGGATTCGGCACGCGCCGCATTTGCGCCGGCTTGATTCAGCAGGGCAGAGT
TGCCATGCATGCGACAGGCACCCTAGAGCCCACGATCTGCCAAGACGCGACGCAGGATATTGATGTCGAAAGTTTGGTGT
TGCGCGTTCAAGGAGTGGATTGGCCATACCAACCCAAGGCCTATGTCATGCTCCATAAGCCTGCAGGAACCGAGTGTTCG
CAGAAGCCATCGGCCTATCCCAGCATCTACACGTTGTTGCCCGCTCCGCTTAGGCAGAGGCCCAGCAAGGGTGCTGTGGA
TGGTGTGCAGGCCGTGGGGCGCTTGGACCAGGACACCACCGGTTTGCTGTTACTCAGTGACGATGGGCAATTCATCCACC
GCATGAGTTCGCCCAAAAAGCATGTGCCAAAGGTGTACCGTGTGACTGCCAAGCATCCCGTGACCAAGGAACAGGTCGAC
CGCTTGCTGGCAGGTGTGGTGCTCGATGATGATCCACGCCCTGTGCGTGCGGCTGCCTGCGCGCTAGTTGACGCGCATGT
GCTTGACCTGACCCTGACGGAAGGGAAGTACCACCAAGTGAAGCGCATGATCGCCGCGGTAGGCAATCGGGTGGAAGCCT
TGCATCGGTCGCACATCGGCCGTTTGGCATTGCCGGCGGATATGCAGCCGGGGGAGTGGCGTTGGCTCGGCGAAGAGGAA
CTCGCCCAGCTGCGGTAG

Upstream 100 bases:

>100_bases
CGTGTGAAAGTAGGTCATCGTCAGGCTCTTACAGCCCAAACCGCCCTCCCACTAAAACAGTGGGAGGGCGTTTTGCTTTG
CAGCGATAACGTCGTATTTC

Downstream 100 bases:

>100_bases
CGAAAGACAGACAAGTGGTCTTCGCCCGTCGCGATCCAATAGGCGATTGCGTCAACGTCGCGCACATCGCGTCCGTTGTC
TGCAAGTCACTTCTGTGAGT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MQLQDILYSQGFGTRRICAGLIQQGRVAMHATGTLEPTICQDATQDIDVESLVLRVQGVDWPYQPKAYVMLHKPAGTECS
QKPSAYPSIYTLLPAPLRQRPSKGAVDGVQAVGRLDQDTTGLLLLSDDGQFIHRMSSPKKHVPKVYRVTAKHPVTKEQVD
RLLAGVVLDDDPRPVRAAACALVDAHVLDLTLTEGKYHQVKRMIAAVGNRVEALHRSHIGRLALPADMQPGEWRWLGEEE
LAQLR

Sequences:

>Translated_245_residues
MQLQDILYSQGFGTRRICAGLIQQGRVAMHATGTLEPTICQDATQDIDVESLVLRVQGVDWPYQPKAYVMLHKPAGTECS
QKPSAYPSIYTLLPAPLRQRPSKGAVDGVQAVGRLDQDTTGLLLLSDDGQFIHRMSSPKKHVPKVYRVTAKHPVTKEQVD
RLLAGVVLDDDPRPVRAAACALVDAHVLDLTLTEGKYHQVKRMIAAVGNRVEALHRSHIGRLALPADMQPGEWRWLGEEE
LAQLR
>Mature_245_residues
MQLQDILYSQGFGTRRICAGLIQQGRVAMHATGTLEPTICQDATQDIDVESLVLRVQGVDWPYQPKAYVMLHKPAGTECS
QKPSAYPSIYTLLPAPLRQRPSKGAVDGVQAVGRLDQDTTGLLLLSDDGQFIHRMSSPKKHVPKVYRVTAKHPVTKEQVD
RLLAGVVLDDDPRPVRAAACALVDAHVLDLTLTEGKYHQVKRMIAAVGNRVEALHRSHIGRLALPADMQPGEWRWLGEEE
LAQLR

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=177, Percent_Identity=42.3728813559322, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1787524, Length=238, Percent_Identity=28.1512605042017, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081838, Length=148, Percent_Identity=34.4594594594595, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790453, Length=248, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27106; Mature: 27106

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLQDILYSQGFGTRRICAGLIQQGRVAMHATGTLEPTICQDATQDIDVESLVLRVQGVD
CCHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHCCCCCCCCHHHHEEEEECCC
WPYQPKAYVMLHKPAGTECSQKPSAYPSIYTLLPAPLRQRPSKGAVDGVQAVGRLDQDTT
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCC
GLLLLSDDGQFIHRMSSPKKHVPKVYRVTAKHPVTKEQVDRLLAGVVLDDDPRPVRAAAC
EEEEECCCCHHHHHHCCCHHHCCHHEEECCCCCCCHHHHHHHHHCCEECCCCCHHHHHHH
ALVDAHVLDLTLTEGKYHQVKRMIAAVGNRVEALHRSHIGRLALPADMQPGEWRWLGEEE
HHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEECCHHH
LAQLR
HHHCC
>Mature Secondary Structure
MQLQDILYSQGFGTRRICAGLIQQGRVAMHATGTLEPTICQDATQDIDVESLVLRVQGVD
CCHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHCCCCCCCCHHHHEEEEECCC
WPYQPKAYVMLHKPAGTECSQKPSAYPSIYTLLPAPLRQRPSKGAVDGVQAVGRLDQDTT
CCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCCCC
GLLLLSDDGQFIHRMSSPKKHVPKVYRVTAKHPVTKEQVDRLLAGVVLDDDPRPVRAAAC
EEEEECCCCHHHHHHCCCHHHCCHHEEECCCCCCCHHHHHHHHHCCEECCCCCHHHHHHH
ALVDAHVLDLTLTEGKYHQVKRMIAAVGNRVEALHRSHIGRLALPADMQPGEWRWLGEEE
HHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEECCHHH
LAQLR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]