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The map label for this gene is ogt [H]

Identifier: 222110233

GI number: 222110233

Start: 1059757

End: 1060287

Strand: Direct

Name: ogt [H]

Synonym: Dtpsy_1019

Alternate gene names: 222110233

Gene position: 1059757-1060287 (Clockwise)

Preceding gene: 222110232

Following gene: 222110234

Centisome position: 27.91

GC content: 67.8

Gene sequence:

>531_bases
ATGCTGTATGCGCAGACCGTGCCTACTCCCTTGGGCGAGATGCTGGCCGTGGCTTCAGACCGGGGGCTGTGTCTGCTGGA
GTTCGTGGGGCAGCCGGGTGTGGAGCGTGAGTTGGCCCAGGTGCAGGCTGCTCGCGGTGAGGTCCTGCGGGAGAAGGGCA
ATGCGGTCACCGTGCTGCTGGCCAGTGAGTTGGCGGAATACTTCGGGCGCCGCCGCCGCGTGTTCGGTGTGCCGCTGGAC
CTGGTGGGCACGCCATTCCAGATCGCCGCGTGGCAGGCGCTGCTGGCCATCCCTTACGGCGAGACGCGCAGCTATGCACA
GCAGGCGCGGACCATCGACCAGCCCACGGCCACGCGCGCCGTCGCTGCGGCCAACGGGCAGAACAAGGTCTCCATCGTCG
TGCCCTGCCACCGGGTGATCGGCAGCAACGGCAGCTTGACCGGCTTTGGCGGCGGCCTGCCGCGCAAGCGTGCGCTGCTG
GCGTTGGAAGATACTTCCGCGCAGCGCGATTGGTTGAACGAGACGCCATAA

Upstream 100 bases:

>100_bases
CCATCGCCGACACGGTGACGGAGATGAAGCTCATCAAGCATGCGTTCCAGGCGGGCATTCCCGCGCAGCGCGGCATCGAG
GACTGAGGGGGCGCCGCGCC

Downstream 100 bases:

>100_bases
CGGCCCCCTCGCCCACACCGCCGCCGAACGCGCGGCCTTTTATCGCGCCGTTGGCGCGCACCGCCAGCGCCGTCCAGAAC
CTCTGTCTGCAGCGCGAGCG

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MLYAQTVPTPLGEMLAVASDRGLCLLEFVGQPGVERELAQVQAARGEVLREKGNAVTVLLASELAEYFGRRRRVFGVPLD
LVGTPFQIAAWQALLAIPYGETRSYAQQARTIDQPTATRAVAAANGQNKVSIVVPCHRVIGSNGSLTGFGGGLPRKRALL
ALEDTSAQRDWLNETP

Sequences:

>Translated_176_residues
MLYAQTVPTPLGEMLAVASDRGLCLLEFVGQPGVERELAQVQAARGEVLREKGNAVTVLLASELAEYFGRRRRVFGVPLD
LVGTPFQIAAWQALLAIPYGETRSYAQQARTIDQPTATRAVAAANGQNKVSIVVPCHRVIGSNGSLTGFGGGLPRKRALL
ALEDTSAQRDWLNETP
>Mature_176_residues
MLYAQTVPTPLGEMLAVASDRGLCLLEFVGQPGVERELAQVQAARGEVLREKGNAVTVLLASELAEYFGRRRRVFGVPLD
LVGTPFQIAAWQALLAIPYGETRSYAQQARTIDQPTATRAVAAANGQNKVSIVVPCHRVIGSNGSLTGFGGGLPRKRALL
ALEDTSAQRDWLNETP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=165, Percent_Identity=29.6969696969697, Blast_Score=65, Evalue=3e-11,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=49.5238095238095, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1788542, Length=165, Percent_Identity=38.1818181818182, Blast_Score=97, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=92, Percent_Identity=39.1304347826087, Blast_Score=78, Evalue=8e-16,
Organism=Drosophila melanogaster, GI17137554, Length=114, Percent_Identity=36.8421052631579, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18914; Mature: 18914

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00374 MGMT ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYAQTVPTPLGEMLAVASDRGLCLLEFVGQPGVERELAQVQAARGEVLREKGNAVTVLL
CCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEH
ASELAEYFGRRRRVFGVPLDLVGTPFQIAAWQALLAIPYGETRSYAQQARTIDQPTATRA
HHHHHHHHHHCCEEECCCHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
VAAANGQNKVSIVVPCHRVIGSNGSLTGFGGGLPRKRALLALEDTSAQRDWLNETP
HEECCCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHEEEEEECCCCHHCCCCCCC
>Mature Secondary Structure
MLYAQTVPTPLGEMLAVASDRGLCLLEFVGQPGVERELAQVQAARGEVLREKGNAVTVLL
CCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEH
ASELAEYFGRRRRVFGVPLDLVGTPFQIAAWQALLAIPYGETRSYAQQARTIDQPTATRA
HHHHHHHHHHCCEEECCCHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
VAAANGQNKVSIVVPCHRVIGSNGSLTGFGGGLPRKRALLALEDTSAQRDWLNETP
HEECCCCCEEEEEEECHHHHCCCCCCCCCCCCCCCHHEEEEEECCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]