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The map label for this gene is mlaA [H]

Identifier: 222109934

GI number: 222109934

Start: 750920

End: 751672

Strand: Direct

Name: mlaA [H]

Synonym: Dtpsy_0719

Alternate gene names: 222109934

Gene position: 750920-751672 (Clockwise)

Preceding gene: 222109933

Following gene: 222109935

Centisome position: 19.78

GC content: 66.8

Gene sequence:

>753_bases
ATGACAAATCAACCTCGCTTCACGGGCTGGACTGCCCGCTGGGCGGCGCTGTTGCTCGGCGCGGCCCTCATGAGCGGCTG
CGCCACGGGCCCCAACGCCAACCCCGCAGATCCGTTCGAACCCTTCAACCGCGGCGTGCATTCGTTCAACGACAGTGTGG
ACAAGGCGGTGCTCAAACCCGTCGCCACCGTTTACCGCGACGTGACGCCGCAGCCGGTGCGTACGGGCGTGAGCAACTTC
TTTGGCAACCTGGGCGATGCCTGGTCGTTCGTCAACAACCTGCTGCAGTTCCGCGGGCTGGAGGCTTACGAGAGCCTCGT
GCGCTTCACCACCAACACCTTCTTCGGGCTGGGCGGCGTACTGGACATTGCCAGCGAGGCAGGCATCGAGCGGCACAAGC
AGGACTTTGGCCTGACGCTGGGCCGCTGGGGCGTGCCCACGGGGCCGTATCTCGTGCTGCCGCTGCTGGGGCCGTCCACC
GTGCGCGACACGGCCGCTTTGCCGGTAGACAGTTGGGGCAACCCGCTGGGCCAGCTCGACCCGGTGGAGGTCCGCAATTC
CCTGTACGGCCTGCGCCTTGTCGATCAGCGCGCTAGCTTGCTGCGCGCGGGCTCGGTGCTGGATACGGCGGCCCTGGACC
CCTACAGCTTCACGCGCGACGTGTACCTCAAGGTGCGTAGTGGCGTGTCAGGCGCACGCGACGAGGACGCCTATGAGGAA
AACGGGGGCAAGCTGCCCGACGACGCGTACTGA

Upstream 100 bases:

>100_bases
TGACCGCCACCCAATCCGCGGTAGTGCTGGAAAACCTGATCGGCCAATTCCTGTATGGCAAGGCCGAAGAAGGTGCTTCG
TCCAATGGAGGTACCAAGAA

Downstream 100 bases:

>100_bases
AAACCCGCAAAGGCCGGCCTGACAAGCACTCGGGCCCCTGGCTGACAGGCGCGGTTGCAGTTGCTTGCATCCCTGCACGG
CGGCGACGCATGTGCAGCGC

Product: vacj family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTNQPRFTGWTARWAALLLGAALMSGCATGPNANPADPFEPFNRGVHSFNDSVDKAVLKPVATVYRDVTPQPVRTGVSNF
FGNLGDAWSFVNNLLQFRGLEAYESLVRFTTNTFFGLGGVLDIASEAGIERHKQDFGLTLGRWGVPTGPYLVLPLLGPST
VRDTAALPVDSWGNPLGQLDPVEVRNSLYGLRLVDQRASLLRAGSVLDTAALDPYSFTRDVYLKVRSGVSGARDEDAYEE
NGGKLPDDAY

Sequences:

>Translated_250_residues
MTNQPRFTGWTARWAALLLGAALMSGCATGPNANPADPFEPFNRGVHSFNDSVDKAVLKPVATVYRDVTPQPVRTGVSNF
FGNLGDAWSFVNNLLQFRGLEAYESLVRFTTNTFFGLGGVLDIASEAGIERHKQDFGLTLGRWGVPTGPYLVLPLLGPST
VRDTAALPVDSWGNPLGQLDPVEVRNSLYGLRLVDQRASLLRAGSVLDTAALDPYSFTRDVYLKVRSGVSGARDEDAYEE
NGGKLPDDAY
>Mature_249_residues
TNQPRFTGWTARWAALLLGAALMSGCATGPNANPADPFEPFNRGVHSFNDSVDKAVLKPVATVYRDVTPQPVRTGVSNFF
GNLGDAWSFVNNLLQFRGLEAYESLVRFTTNTFFGLGGVLDIASEAGIERHKQDFGLTLGRWGVPTGPYLVLPLLGPSTV
RDTAALPVDSWGNPLGQLDPVEVRNSLYGLRLVDQRASLLRAGSVLDTAALDPYSFTRDVYLKVRSGVSGARDEDAYEEN
GGKLPDDAY

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=244, Percent_Identity=34.8360655737705, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 27085; Mature: 26953

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQPRFTGWTARWAALLLGAALMSGCATGPNANPADPFEPFNRGVHSFNDSVDKAVLKP
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VATVYRDVTPQPVRTGVSNFFGNLGDAWSFVNNLLQFRGLEAYESLVRFTTNTFFGLGGV
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LDIASEAGIERHKQDFGLTLGRWGVPTGPYLVLPLLGPSTVRDTAALPVDSWGNPLGQLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCCC
PVEVRNSLYGLRLVDQRASLLRAGSVLDTAALDPYSFTRDVYLKVRSGVSGARDEDAYEE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
NGGKLPDDAY
CCCCCCCCCC
>Mature Secondary Structure 
TNQPRFTGWTARWAALLLGAALMSGCATGPNANPADPFEPFNRGVHSFNDSVDKAVLKP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VATVYRDVTPQPVRTGVSNFFGNLGDAWSFVNNLLQFRGLEAYESLVRFTTNTFFGLGGV
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LDIASEAGIERHKQDFGLTLGRWGVPTGPYLVLPLLGPSTVRDTAALPVDSWGNPLGQLD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCCC
PVEVRNSLYGLRLVDQRASLLRAGSVLDTAALDPYSFTRDVYLKVRSGVSGARDEDAYEE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHH
NGGKLPDDAY
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]