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The map label for this gene is mlaE [H]

Identifier: 222109932

GI number: 222109932

Start: 749621

End: 750403

Strand: Direct

Name: mlaE [H]

Synonym: Dtpsy_0717

Alternate gene names: 222109932

Gene position: 749621-750403 (Clockwise)

Preceding gene: 222109931

Following gene: 222109933

Centisome position: 19.74

GC content: 67.82

Gene sequence:

>783_bases
ATGAGCTGGTGGCGTCCCACGCACGTAGGCTACGCCGTGCGCACGAAGCTGGCCGATGTGGGCATGGGCGCGCGCCTGTT
CGCGCGGCTGCTGGGGCTGTTCGGCGACGTGATGCGCCGCCCTGGCCTGGTGCGTGACCAGGTGCACTTCCTGGGCAACT
ATTCGCTGTCCATCATCGCCATGTCGGGCCTGTTTGTGGGCTTCGTGCTTGCGCTGCAGGGCTACAACGTGCTGCAGCTC
TATGGCTCGGCCAACTCACTGGGCCTGGTGGTCACGTTGGGCCTGGTGCGCGAGCTGGGGCCGGTGGTCACGGCGCTGCT
GTTTGCCGGACGCGCGGGCACGTCGCTCACGGCCGAGATCGGTTTGATGCGCGCCGGCGAACAGCTGTCGGCCATGGAGA
TGATGGCCGTGGACCCGGTGCGCCGCATCCTGGCGCCGCGCTTTTGGGGGGGCGTGATCGCCATGCCGCTGCTGGCACTG
GTGTTCAACGCGGTGGGTGTGATCGGTGGCTGGCTGGTCGGCGTGGTGCTCATCGGCGTGGACGGCGGGGCGTTCTGGGG
CCAGATGCAAAGCGGTGTCGACGTCTGGAAGGACGTGGGCAACGGCATCGTCAAGAGCCTCGCGTTCGGCGTGGCCGTCA
CCTTCATCGCGGTGCTGCAGGGCTTCGTGGCCAAGCCGACCCCGGAAGGCGTCTCGCGCGCCACCACGCGCACGGTGGTG
ATGGCGTCGCTGTCCGTGCTGGGGCTGGACTTCGTCCTCACCGCCTTGATGTTCAGTATCTGA

Upstream 100 bases:

>100_bases
CCGGGCCGGTGCCGTTCCATTACCCCGGCCCCAGCGTGGCCGACGATTTCGGCCCCTTGCGCTCGCCCAGCGTGGACGCC
CACGCCAGCGGAGGTACCCC

Downstream 100 bases:

>100_bases
CGGCGACCATGCGTCTGTCTTGAGGAAATTCACCATGCAGCATTCCAAAAACGATTTCTGGGTGGGGCTGTTCGTCCTGC
TGGGCGGAGCGGCGCTGGTC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSWWRPTHVGYAVRTKLADVGMGARLFARLLGLFGDVMRRPGLVRDQVHFLGNYSLSIIAMSGLFVGFVLALQGYNVLQL
YGSANSLGLVVTLGLVRELGPVVTALLFAGRAGTSLTAEIGLMRAGEQLSAMEMMAVDPVRRILAPRFWGGVIAMPLLAL
VFNAVGVIGGWLVGVVLIGVDGGAFWGQMQSGVDVWKDVGNGIVKSLAFGVAVTFIAVLQGFVAKPTPEGVSRATTRTVV
MASLSVLGLDFVLTALMFSI

Sequences:

>Translated_260_residues
MSWWRPTHVGYAVRTKLADVGMGARLFARLLGLFGDVMRRPGLVRDQVHFLGNYSLSIIAMSGLFVGFVLALQGYNVLQL
YGSANSLGLVVTLGLVRELGPVVTALLFAGRAGTSLTAEIGLMRAGEQLSAMEMMAVDPVRRILAPRFWGGVIAMPLLAL
VFNAVGVIGGWLVGVVLIGVDGGAFWGQMQSGVDVWKDVGNGIVKSLAFGVAVTFIAVLQGFVAKPTPEGVSRATTRTVV
MASLSVLGLDFVLTALMFSI
>Mature_259_residues
SWWRPTHVGYAVRTKLADVGMGARLFARLLGLFGDVMRRPGLVRDQVHFLGNYSLSIIAMSGLFVGFVLALQGYNVLQLY
GSANSLGLVVTLGLVRELGPVVTALLFAGRAGTSLTAEIGLMRAGEQLSAMEMMAVDPVRRILAPRFWGGVIAMPLLALV
FNAVGVIGGWLVGVVLIGVDGGAFWGQMQSGVDVWKDVGNGIVKSLAFGVAVTFIAVLQGFVAKPTPEGVSRATTRTVVM
ASLSVLGLDFVLTALMFSI

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=236, Percent_Identity=58.8983050847458, Blast_Score=255, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27514; Mature: 27383

Theoretical pI: Translated: 10.65; Mature: 10.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SWWRPTHVGYAVRTKLADVGMGARLFARLLGLFGDVMRRPGLVRDQVHFLGNYSLSIIA
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHH
MSGLFVGFVLALQGYNVLQLYGSANSLGLVVTLGLVRELGPVVTALLFAGRAGTSLTAEI
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GLMRAGEQLSAMEMMAVDPVRRILAPRFWGGVIAMPLLALVFNAVGVIGGWLVGVVLIGV
HHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGGAFWGQMQSGVDVWKDVGNGIVKSLAFGVAVTFIAVLQGFVAKPTPEGVSRATTRTVV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
MASLSVLGLDFVLTALMFSI
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]