Click here to switch to the map view.

The map label for this gene is narH [H]

Identifier: 222109648

GI number: 222109648

Start: 440939

End: 442462

Strand: Direct

Name: narH [H]

Synonym: Dtpsy_0428

Alternate gene names: 222109648

Gene position: 440939-442462 (Clockwise)

Preceding gene: 222109647

Following gene: 222109649

Centisome position: 11.61

GC content: 64.7

Gene sequence:

>1524_bases
ATGAAAATTCGCGCACAAATCGGCATGGTGCTGAACCTGGACAAGTGCATTGGTTGCCACACCTGTTCCGTCACCTGCAA
GAACGTCTGGACCAGCCGTCCCGGCGTGGAATACGCCTGGTTCAATAACGTCGAGACCAAGCCCGGCATCGGCTATCCCA
AGGAATGGGAAAACCAGGACAAGTGGAACGGCGGCTGGGTGCGCAACCCGGACGGCTCCATCGCCCCCCGCCAGGGCGGC
AAGTGGAAGCTGCTCATGCGCATCTTCGCCAACCCTAACCTGCCGCAGATCGACGACTACTACGAGCCCTTCACCTTCGA
CTACGACCACCTGCAGTCGGCGCCCGAGATGAAGGCCGCGCCCACGGCGCGCCCGCGCAGCCTGATCACGGGCCAGCGCA
TGGAAAAGATCGAATGGGGCCCGAACTGGGAGGAAATCCTGGGCGGCGAGTTCTCCAAGCGCAGCGCCGACGTGAACTTC
GAGCAGGTCCAGAAGGACATGTACGGCCAGTTCGAGAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAGCACTGCCT
GAACCCGGCGTGCGTGGCATCGTGCCCCTCGGGCTCGATCTACAAGCGCGAGGAAGACGGCATCGTGCTGATCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCGTCTCGGGCTGCCCCTACAAGAAGATCTATTACAACTGGCAGACCGGCAAGGCC
GAGAAGTGCATCTTCTGCTACCCGCGCATCGAGGCGGGGCAGCCCACGGTGTGCTCCGAGACCTGCGTGGGCCGCATCCG
CTACCTGGGCGTGCTGCTGTATGACGCCGACCGCATCCAGGAAGCCGCCAGCGTGGAGCGCGACCGCGACCTGTACGAGG
CGCAGCTGGACATCTTCCTGGACCCCAACGACCCGGACGTGATCCGCCAGGCCAAAGCCGACGGCATCTCCGACGCCTGG
CTGGAAGCCGCCAAGAACAGCCCCGTCTACAAGATGGCCGTGGACTGGAAGATCGCACTGCCGCTGCACCCCGAGTACCG
CACACTGCCCATGGTGTGGTACGTGCCGCCGCTGTCGCCCATCACCTCGGCGGCCAACGCCGGCCACCTGGGCGTGAATG
GCGAGATTCCCGACGTGTCGCAGCTGCGCATCCCCGTGCAGTACCTGGCCAACCTGCTCACCGCCGGCGACACCGGCCCC
GTGGTGCGGGCGCTGGAGCGCATGCTGGCCATGCGCGCCTACCAGCGCGGTATGCATGTGGACCGCCAGCAGAACCTGGC
CGTGCTGCAACAGGTGGGGCTCACGGTGCATGAAGTGGAAGACATGTACCAGGTCATGGCGATCGCCAACTACGAAGACC
GCTTCGTCATCCCCTCGGCGCACCGCGAGTACGCCGAGAACGCATTCGACCTGCGCGGCGGCTGCGGCTTCTCGTTCGGC
AATGGCTGCTCCGACGGCTCCACGGACGTGAGCATGTTCGGTGGCAAGAAGCGACGCACCATCCCCATCAAGGCCACGGT
CTGA

Upstream 100 bases:

>100_bases
CGACGAGTTCGTGCTGGTGCGCAAGATGCGCCGCGTCGACTGGATGGACGACGAGGCATCCACCAGCGCCGCCCACGCCT
GAGCGACAAGGAGAGACACG

Downstream 100 bases:

>100_bases
GGCGAAAGGAAGAATTGCCATGTTCCAGAAAACGCCTGAATCCCTGCGCCTGACGCTGCGTGCGCTGGCGCACCTGCTGG
GCTATCCCGATGCCGCGCTG

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGVEYAWFNNVETKPGIGYPKEWENQDKWNGGWVRNPDGSIAPRQGG
KWKLLMRIFANPNLPQIDDYYEPFTFDYDHLQSAPEMKAAPTARPRSLITGQRMEKIEWGPNWEEILGGEFSKRSADVNF
EQVQKDMYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQTGKA
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIQEAASVERDRDLYEAQLDIFLDPNDPDVIRQAKADGISDAW
LEAAKNSPVYKMAVDWKIALPLHPEYRTLPMVWYVPPLSPITSAANAGHLGVNGEIPDVSQLRIPVQYLANLLTAGDTGP
VVRALERMLAMRAYQRGMHVDRQQNLAVLQQVGLTVHEVEDMYQVMAIANYEDRFVIPSAHREYAENAFDLRGGCGFSFG
NGCSDGSTDVSMFGGKKRRTIPIKATV

Sequences:

>Translated_507_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGVEYAWFNNVETKPGIGYPKEWENQDKWNGGWVRNPDGSIAPRQGG
KWKLLMRIFANPNLPQIDDYYEPFTFDYDHLQSAPEMKAAPTARPRSLITGQRMEKIEWGPNWEEILGGEFSKRSADVNF
EQVQKDMYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQTGKA
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIQEAASVERDRDLYEAQLDIFLDPNDPDVIRQAKADGISDAW
LEAAKNSPVYKMAVDWKIALPLHPEYRTLPMVWYVPPLSPITSAANAGHLGVNGEIPDVSQLRIPVQYLANLLTAGDTGP
VVRALERMLAMRAYQRGMHVDRQQNLAVLQQVGLTVHEVEDMYQVMAIANYEDRFVIPSAHREYAENAFDLRGGCGFSFG
NGCSDGSTDVSMFGGKKRRTIPIKATV
>Mature_507_residues
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGVEYAWFNNVETKPGIGYPKEWENQDKWNGGWVRNPDGSIAPRQGG
KWKLLMRIFANPNLPQIDDYYEPFTFDYDHLQSAPEMKAAPTARPRSLITGQRMEKIEWGPNWEEILGGEFSKRSADVNF
EQVQKDMYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQTGKA
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIQEAASVERDRDLYEAQLDIFLDPNDPDVIRQAKADGISDAW
LEAAKNSPVYKMAVDWKIALPLHPEYRTLPMVWYVPPLSPITSAANAGHLGVNGEIPDVSQLRIPVQYLANLLTAGDTGP
VVRALERMLAMRAYQRGMHVDRQQNLAVLQQVGLTVHEVEDMYQVMAIANYEDRFVIPSAHREYAENAFDLRGGCGFSFG
NGCSDGSTDVSMFGGKKRRTIPIKATV

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=70.9486166007905, Blast_Score=779, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=504, Percent_Identity=70.8333333333333, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=167, Percent_Identity=32.3353293413174, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787122, Length=167, Percent_Identity=31.7365269461078, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI2367345, Length=142, Percent_Identity=27.4647887323944, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789370, Length=106, Percent_Identity=37.7358490566038, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=35.5555555555556, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57353; Mature: 57353

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGVEYAWFNNVETKPGIGYPKEWENQD
CEEHEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWVRNPDGSIAPRQGGKWKLLMRIFANPNLPQIDDYYEPFTFDYDHLQSAPEMKAA
CCCCCEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPRSLITGQRMEKIEWGPNWEEILGGEFSKRSADVNFEQVQKDMYGQFENTFMMYLP
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQTGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCC
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIQEAASVERDRDLYEAQLDIFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHEEEEEEEEE
DPNDPDVIRQAKADGISDAWLEAAKNSPVYKMAVDWKIALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCH
ITSAANAGHLGVNGEIPDVSQLRIPVQYLANLLTAGDTGPVVRALERMLAMRAYQRGMHV
HHHCCCCCCCCCCCCCCCHHHHEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
DRQQNLAVLQQVGLTVHEVEDMYQVMAIANYEDRFVIPSAHREYAENAFDLRGGCGFSFG
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCC
NGCSDGSTDVSMFGGKKRRTIPIKATV
CCCCCCCCCHHHCCCCCCEEEEEEECC
>Mature Secondary Structure
MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGVEYAWFNNVETKPGIGYPKEWENQD
CEEHEEECEEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWNGGWVRNPDGSIAPRQGGKWKLLMRIFANPNLPQIDDYYEPFTFDYDHLQSAPEMKAA
CCCCCEEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCC
PTARPRSLITGQRMEKIEWGPNWEEILGGEFSKRSADVNFEQVQKDMYGQFENTFMMYLP
CCCCCHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRGWRMCVSGCPYKKIYYNWQTGKA
HHHHHHCCCCEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCEEEEEECCCCC
EKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDADRIQEAASVERDRDLYEAQLDIFL
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHHEEEEEEEEE
DPNDPDVIRQAKADGISDAWLEAAKNSPVYKMAVDWKIALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCEEEEEEECCCCH
ITSAANAGHLGVNGEIPDVSQLRIPVQYLANLLTAGDTGPVVRALERMLAMRAYQRGMHV
HHHCCCCCCCCCCCCCCCHHHHEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
DRQQNLAVLQQVGLTVHEVEDMYQVMAIANYEDRFVIPSAHREYAENAFDLRGGCGFSFG
CHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCC
NGCSDGSTDVSMFGGKKRRTIPIKATV
CCCCCCCCCHHHCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]