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The map label for this gene is narQ [H]

Identifier: 222109643

GI number: 222109643

Start: 431593

End: 433509

Strand: Direct

Name: narQ [H]

Synonym: Dtpsy_0423

Alternate gene names: 222109643

Gene position: 431593-433509 (Clockwise)

Preceding gene: 222109642

Following gene: 222109644

Centisome position: 11.37

GC content: 67.4

Gene sequence:

>1917_bases
ATGCGCAACAAGTCCACGCTGTCCACCAAGCTGCTGGCCATGGGCACGGTGTTCCTGCTGGTGGCGCTGGCATCCATCGG
CTTCACGCTGTGGGTGACCTGGAAGTTGGAGGGGGGCGCCGCTGCGGTGAACGAGGCGGGCCGCCTGCGCATGAACATGC
TGCGCATGGTGCTGGCCCAGCAGAACGAGTCGCCCCAGGAATTCGCGCGGCTGGAAACGCGCTTCAACGAAGGGCTGGAG
TTGCTGCGCACGGGTGACCCCGCCCGGCCGCTGTTCGTGCCCTGGAGCGATGACACGCGCGAGCGCTACGAACACATCCG
GCGGCAGTGGCTGCTCATCGAAAACGACTGGAAAGCCGCCGCGCACTATGAAAGCACCGCCGCGGCCGTGGCGCGCGCCG
ATGCCTTCGTGCTGGACCTGGACAGCTTTGTCGAGGCCATCGAGATCCAGATCGTGCGCCTCACGGCGGTGCTGCATCTG
TTCCACCTGTTCATGGTGGCGTTGGCCATCGGCTCGGCTGTGGCCTTCATGGCGCTGAGCTACCTGCTCGTACTCAACCC
CGTCACGCGGCTGCAACAGGCCCTGGCGCGGTTGCGCAAGGGCGACCTGGGCACGCGCCTGGAGGTGGAAGCCGACGACG
AGTTCGGCCAGCTGACGGCGGGTTTCAACCTCATGGCCCATGCGCTGCAGACCTCGCATGAGGACCTGGAGCGCAAGGTA
CGCGAAAAGACCGCCACCATCGAAGTGCGCAACCAGCGCCTGGCGGCGTTGTATGCGGTGAGCGCCCTTGCGGCGGAGGC
GGGAAGCCTGGAAGAGTTGACCCAGGGTTTCGTGCAGCAGATCCGCCGCGTGGCGGGGTGCGACGCGGCGGCTGTGCGCT
GGTCGGACGAAGCCAACGAGCGCTACGTGCTGCTGGCCTCCGACGGGCTGCCCCAGTCGCTGGTGGAACATGAGCACTGT
GTGGTCACCGGCGCCTGCGAATGCGGACAGGCGCAGGGCCAGGCGCGCATGCGTGTGATTCCGATCATGCCTGCCGGGGG
CCTGCAGTTGCCGCATTGCCGCGAGGCCGGCTTCGAGACGGTGGTGAGTATCCCGGTGCAGATGCACCAGCGCCTGTTGG
GTGAGGTCACGCTGCTGTATCGCAGTCCGCTGGAGCTGTCGGCGGAGATGCGCGAGCTACTGTCCACCATGGCGCACCAC
TTGGCCACTTCCATGGAAAGCCTGCGCGCCTCGGCGCTGGAGCGCGAGGCAGCGGTGGCGCAGGAGCGTAGCCTCATTGC
GCGCGAATTGCACGACTCCATCGCGCAGTCGCTGGCCTTTCTCAAGATCCAAACCCAGCTGCTGCGCGACGCGGTGGCGA
AGGGCAACACCGAGGCGCGTGACCGCAGCATGGCGGAGCTGGATACCGGTGTGCGCGAATGCTATGCCGACGTGCGTGAG
CTGCTGGTGCACTTTCGCACGCGCACGCAGGACGAGGACATCGAAGCGGCGCTGCGCGCCACGCTCTCGAAGTTCGAACA
CCAGACCGGCGCGGCCACGGCGCTGAGCATGGCGGGGCACGGCCTGCCGCTGGCGCAGGACGTGCAGATCCAGGTGCTGC
ACATCCTGCAGGAGGCCTTGTCCAACGTGCGCAAGCACGCCGATGCACGCCGCGTGGAGCTGCGGGTGCAGCGTCACCCC
CGCTGGCGCTTCGAGGTGCAGGACGATGGCCGCGGGTTCGACCCGGACAGCGTGCCACCCGATTCGCTGCATGTGGGCCT
GGGCATCATGCGTGAACGCGCGCAGCGCATTGGTGCGCATCTGGAGCTGGTTTCGCGCCCCGGCGGCGGTGGCACCTGCG
TAATCCTGGAGCTGCCACCAACCACCCGCGAGGAGCCTGCGTTGCCCCTCTCCCTTGTTTCTTCCGCCGCCGCATGA

Upstream 100 bases:

>100_bases
GCGCGCGAAATCATGTTTTCGCTTGTATGCCGCGGCCGTAGGCTTGAGCAAACTTGACTTCCATCAAGACATTTCAAAGG
CGTGTGCCTACACTGCCGCC

Downstream 100 bases:

>100_bases
ATGCTCCCACCACGCTTTTCCTGATCGATGACCACACCTTGCTGCGCCGCGGACTGGTGGCGCTGCTGTCCCAGCAGCCG
GACCTGCGCGTGGTGGGCGA

Product: gaf sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 638; Mature: 638

Protein sequence:

>638_residues
MRNKSTLSTKLLAMGTVFLLVALASIGFTLWVTWKLEGGAAAVNEAGRLRMNMLRMVLAQQNESPQEFARLETRFNEGLE
LLRTGDPARPLFVPWSDDTRERYEHIRRQWLLIENDWKAAAHYESTAAAVARADAFVLDLDSFVEAIEIQIVRLTAVLHL
FHLFMVALAIGSAVAFMALSYLLVLNPVTRLQQALARLRKGDLGTRLEVEADDEFGQLTAGFNLMAHALQTSHEDLERKV
REKTATIEVRNQRLAALYAVSALAAEAGSLEELTQGFVQQIRRVAGCDAAAVRWSDEANERYVLLASDGLPQSLVEHEHC
VVTGACECGQAQGQARMRVIPIMPAGGLQLPHCREAGFETVVSIPVQMHQRLLGEVTLLYRSPLELSAEMRELLSTMAHH
LATSMESLRASALEREAAVAQERSLIARELHDSIAQSLAFLKIQTQLLRDAVAKGNTEARDRSMAELDTGVRECYADVRE
LLVHFRTRTQDEDIEAALRATLSKFEHQTGAATALSMAGHGLPLAQDVQIQVLHILQEALSNVRKHADARRVELRVQRHP
RWRFEVQDDGRGFDPDSVPPDSLHVGLGIMRERAQRIGAHLELVSRPGGGGTCVILELPPTTREEPALPLSLVSSAAA

Sequences:

>Translated_638_residues
MRNKSTLSTKLLAMGTVFLLVALASIGFTLWVTWKLEGGAAAVNEAGRLRMNMLRMVLAQQNESPQEFARLETRFNEGLE
LLRTGDPARPLFVPWSDDTRERYEHIRRQWLLIENDWKAAAHYESTAAAVARADAFVLDLDSFVEAIEIQIVRLTAVLHL
FHLFMVALAIGSAVAFMALSYLLVLNPVTRLQQALARLRKGDLGTRLEVEADDEFGQLTAGFNLMAHALQTSHEDLERKV
REKTATIEVRNQRLAALYAVSALAAEAGSLEELTQGFVQQIRRVAGCDAAAVRWSDEANERYVLLASDGLPQSLVEHEHC
VVTGACECGQAQGQARMRVIPIMPAGGLQLPHCREAGFETVVSIPVQMHQRLLGEVTLLYRSPLELSAEMRELLSTMAHH
LATSMESLRASALEREAAVAQERSLIARELHDSIAQSLAFLKIQTQLLRDAVAKGNTEARDRSMAELDTGVRECYADVRE
LLVHFRTRTQDEDIEAALRATLSKFEHQTGAATALSMAGHGLPLAQDVQIQVLHILQEALSNVRKHADARRVELRVQRHP
RWRFEVQDDGRGFDPDSVPPDSLHVGLGIMRERAQRIGAHLELVSRPGGGGTCVILELPPTTREEPALPLSLVSSAAA
>Mature_638_residues
MRNKSTLSTKLLAMGTVFLLVALASIGFTLWVTWKLEGGAAAVNEAGRLRMNMLRMVLAQQNESPQEFARLETRFNEGLE
LLRTGDPARPLFVPWSDDTRERYEHIRRQWLLIENDWKAAAHYESTAAAVARADAFVLDLDSFVEAIEIQIVRLTAVLHL
FHLFMVALAIGSAVAFMALSYLLVLNPVTRLQQALARLRKGDLGTRLEVEADDEFGQLTAGFNLMAHALQTSHEDLERKV
REKTATIEVRNQRLAALYAVSALAAEAGSLEELTQGFVQQIRRVAGCDAAAVRWSDEANERYVLLASDGLPQSLVEHEHC
VVTGACECGQAQGQARMRVIPIMPAGGLQLPHCREAGFETVVSIPVQMHQRLLGEVTLLYRSPLELSAEMRELLSTMAHH
LATSMESLRASALEREAAVAQERSLIARELHDSIAQSLAFLKIQTQLLRDAVAKGNTEARDRSMAELDTGVRECYADVRE
LLVHFRTRTQDEDIEAALRATLSKFEHQTGAATALSMAGHGLPLAQDVQIQVLHILQEALSNVRKHADARRVELRVQRHP
RWRFEVQDDGRGFDPDSVPPDSLHVGLGIMRERAQRIGAHLELVSRPGGGGTCVILELPPTTREEPALPLSLVSSAAA

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate/nitrite availability to the narL/narP proteins. NarQ probably activates narL and narP by phosphorylation. NarQ probably negatively regulates the narL protein by dephosphorylation [H]

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=625, Percent_Identity=26.08, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1787474, Length=252, Percent_Identity=31.7460317460317, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082311, Length=219, Percent_Identity=28.7671232876712, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 70584; Mature: 70584

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKSTLSTKLLAMGTVFLLVALASIGFTLWVTWKLEGGAAAVNEAGRLRMNMLRMVLAQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
QNESPQEFARLETRFNEGLELLRTGDPARPLFVPWSDDTRERYEHIRRQWLLIENDWKAA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHEEEEECCCCHH
AHYESTAAAVARADAFVLDLDSFVEAIEIQIVRLTAVLHLFHLFMVALAIGSAVAFMALS
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLLVLNPVTRLQQALARLRKGDLGTRLEVEADDEFGQLTAGFNLMAHALQTSHEDLERKV
HHHHHCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
REKTATIEVRNQRLAALYAVSALAAEAGSLEELTQGFVQQIRRVAGCDAAAVRWSDEANE
HHHHHHEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCC
RYVLLASDGLPQSLVEHEHCVVTGACECGQAQGQARMRVIPIMPAGGLQLPHCREAGFET
EEEEEECCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCHH
VVSIPVQMHQRLLGEVTLLYRSPLELSAEMRELLSTMAHHLATSMESLRASALEREAAVA
HHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QERSLIARELHDSIAQSLAFLKIQTQLLRDAVAKGNTEARDRSMAELDTGVRECYADVRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLVHFRTRTQDEDIEAALRATLSKFEHQTGAATALSMAGHGLPLAQDVQIQVLHILQEAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SNVRKHADARRVELRVQRHPRWRFEVQDDGRGFDPDSVPPDSLHVGLGIMRERAQRIGAH
HHHHHHCCHHHHHHEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHH
LELVSRPGGGGTCVILELPPTTREEPALPLSLVSSAAA
HEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MRNKSTLSTKLLAMGTVFLLVALASIGFTLWVTWKLEGGAAAVNEAGRLRMNMLRMVLAQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
QNESPQEFARLETRFNEGLELLRTGDPARPLFVPWSDDTRERYEHIRRQWLLIENDWKAA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHEEEEECCCCHH
AHYESTAAAVARADAFVLDLDSFVEAIEIQIVRLTAVLHLFHLFMVALAIGSAVAFMALS
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLLVLNPVTRLQQALARLRKGDLGTRLEVEADDEFGQLTAGFNLMAHALQTSHEDLERKV
HHHHHCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHH
REKTATIEVRNQRLAALYAVSALAAEAGSLEELTQGFVQQIRRVAGCDAAAVRWSDEANE
HHHHHHEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCC
RYVLLASDGLPQSLVEHEHCVVTGACECGQAQGQARMRVIPIMPAGGLQLPHCREAGFET
EEEEEECCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCHH
VVSIPVQMHQRLLGEVTLLYRSPLELSAEMRELLSTMAHHLATSMESLRASALEREAAVA
HHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QERSLIARELHDSIAQSLAFLKIQTQLLRDAVAKGNTEARDRSMAELDTGVRECYADVRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLVHFRTRTQDEDIEAALRATLSKFEHQTGAATALSMAGHGLPLAQDVQIQVLHILQEAL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
SNVRKHADARRVELRVQRHPRWRFEVQDDGRGFDPDSVPPDSLHVGLGIMRERAQRIGAH
HHHHHHCCHHHHHHEEECCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHH
LELVSRPGGGGTCVILELPPTTREEPALPLSLVSSAAA
HEEEECCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1528845; 1508040; 9205837; 9278503 [H]